SSGCID: Target Page

BobuA.19108.a

peptidase (BB_0067)

CENTER ID:	BobuA.19108.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19108.a.B1.GE39578	full length		1	592

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19108.a.B1.PW37855	full length		1	592

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDINKRLALL RDHMRDNGVD AYLVAGYDPH FSEYSHERYS TRKFITGFSG SFGTVIITLS KAVLFTDGRY FLQAEQELKG TEIALIKLGV KGSPDIFTYI NLNLKESKLG IYSDEISIKF YKELSEKCKN THIKALNQDL IDLIWKSRPQ LEFSHVFELA DAEKNNKRAE KIKSIYLILE KNLADFYVIT ALDEIAWVLN LRGADVEESA LFYSFLLISR NKNRKNVLFV DXKKLDSGVK EALEMENFEI ESYSNFYCFL DKIKHEGKFF VSFYANVRVL KVLGETNIIL GESIISSLKA LKTDYELLKM KEAHIIDAIA LIKFLRKFKS LSKVELSELD EIDVADMLLH FRKLNKDFFS SSFDSIVGFR ENGALPHYKP KRGRKINTNG LLLIDSGGSY FGLGTTDVTR VFLIGDASDE EKRDYTLVLK AFIGLSSLKF PYGSSGAFLD GICRLPLLKN ELNFIHGTGH GVGFFLNVHE LPVSISPNSS YLFKGSEVVS IEPGLYRTFS HGIRIENLVF VRQAFTNDFG AFLEFENLTL VPFEKELIVK EMLSEDELNY INDYHECVFL TLKERFDNEG ELEFLAKLTS KI

NT Sequence

ttggatatta ataaaagatt agctttgctt agagatcata tgagagataa tggggttgat gcttatttgg tagcaggcta tgatcctcat tttagtgaat attctcatga aagatatagc actcgtaagt ttattacagg tttttctggt agctttggta cggtaattat aacattatca aaagctgttc tttttacaga tggtaggtat tttttacagg ccgagcaaga gcttaaggga actgaaattg cattaataaa gcttggagta aaaggatctc ctgatatttt tacgtatata aatttgaatc ttaaagaatc aaagcttgga atttattcag atgagattag tataaagttt tataaagagt tgtctgaaaa atgtaaaaat acacatatca aggctttaaa tcaagatcta attgatttaa tttggaaatc tagaccccag cttgagttta gccatgtatt tgaattggct gatgctgaga agaataacaa aagagctgaa aaaatcaaat ctatttattt gatcttagaa aagaatttgg cagattttta tgttattact gctcttgatg agattgcttg ggtgctgaat ttaagagggg cagatgttga agaatcagca ttattttatt cgtttctttt aatatctagg aataaaaatc gaaaaaatgt tctttttgtt gacayaaaaa aacttgattc gggtgttaaa gaagcacttg aaatggaaaa ttttgaaata gagtcttaca gcaattttta ctgttttttg gacaaaataa agcacgaagg taaatttttt gtttcatttt atgccaatgt tagggtttta aaagttcttg gtgagactaa tatcattttg ggagagagta ttataagtag tcttaaggca ttaaaaaccg attatgaact tcttaagatg aaagaagcgc atattattga tgctattgca ttgattaaat ttttacgtaa atttaaaagt cttagcaagg ttgaattgtc tgaattagat gaaatagatg ttgctgatat gcttttgcat tttagaaaat tgaataaaga tttttttagt tctagctttg actcaatagt tggttttaga gaaaatggag ctcttccgca ttataagccc aaaagaggta gaaaaataaa taccaatggt cttcttttga ttgattctgg gggttcatat tttggacttg gtacgacaga cgttacaaga gtttttttaa taggtgatgc ctctgatgaa gaaaagcgcg actatacttt ggttcttaag gctttcattg gcctttcttc tttaaagttt ccatatggat cttccggagc ttttcttgat ggaatttgta gattaccgct tttaaaaaat gaattgaatt ttattcatgg cactgggcat ggtgttgggt tttttcttaa tgttcatgaa cttccagttt ctattagtcc taattctagt tatcttttta aaggatctga ggtagtttca attgagcctg gtctttatag aacatttagt cacggaataa gaattgagaa tttagttttt gtaaggcaag cttttacaaa tgactttgga gcttttttag agtttgagaa tttaaccctt gttccttttg aaaaggaatt gatagtaaaa gaaatgctct cagaagatga gttaaattat atcaatgatt atcatgaatg tgtattttta acattaaaag agcgctttga taatgaagga gagttggaat ttttagcaaa gctaacaagt aaaatatga

Details for BobuA.19108.a.B1.GE39578

HARVESTED ON:	10/30/2015
SEQUENCED ON:	10/23/2015
EXPECTED MW:	69kDa
OBSERVED MW:	69kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMD INKRLALLRD HMRDNGVDAY LVAGYDPHFS EYSHERYSTR KFITGFSGSF GTVIITLSKA VLFTDGRYFL QAEQELKGTE IALIKLGVKG SPDIFTYINL NLKESKLGIY SDEISIKFYK ELSEKCKNTH IKALNQDLID LIWKSRPQLE FSHVFELADA EKNNKRAEKI KSIYLILEKN LADFYVITAL DEIAWVLNLR GADVEESALF YSFLLISRNK NRKNVLFVDX KKLDSGVKEA LEMENFEIES YSNFYCFLDK IKHEGKFFVS FYANVRVLKV LGETNIILGE SIISSLKALK TDYELLKMKE AHIIDAIALI KFLRKFKSLS KVELSELDEI DVADMLLHFR KLNKDFFSSS FDSIVGFREN GALPHYKPKR GRKINTNGLL LIDSGGSYFG LGTTDVTRVF LIGDASDEEK RDYTLVLKAF IGLSSLKFPY GSSGAFLDGI CRLPLLKNEL NFIHGTGHGV GFFLNVHELP VSISPNSSYL FKGSEVVSIE PGLYRTFSHG IRIENLVFVR QAFTNDFGAF LEFENLTLVP FEKELIVKEM LSEDELNYIN DYHECVFLTL KERFDNEGEL EFLAKLTSKI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggata ttaataaaag attagctttg cttagagatc atatgagaga taatggggtt gatgcttatt tggtagcagg ctatgatcct cattttagtg aatattctca tgaaagatat agcactcgta agtttattac aggtttttct ggtagctttg gtacggtaat tataacatta tcaaaagctg ttctttttac agatggtagg tattttttac aggccgagca agagcttaag ggaactgaaa ttgcattaat aaagcttgga gtaaaaggat ctcctgatat ttttacgtat ataaatttga atcttaaaga atcaaagctt ggaatttatt cagatgagat tagtataaag ttttataaag agttgtctga aaaatgtaaa aatacacata tcaaggcttt aaatcaagat ctaattgatt taatttggaa atctagaccc cagcttgagt ttagccatgt atttgaattg gctgatgctg agaagaataa caaaagagct gaaaaaatca aatctattta tttgatctta gaaaagaatt tggcagattt ttatgttatt actgctcttg atgagattgc ttgggtgctg aatttaagag gggcagatgt tgaagaatca gcattatttt attcgtttct tttaatatct aggaataaaa atcgaaaaaa tgttcttttt gttgacayaa aaaaacttga ttcgggtgtt aaagaagcac ttgaaatgga aaattttgaa atagagtctt acagcaattt ttactgtttt ttggacaaaa taaagcacga aggtaaattt tttgtttcat tttatgccaa tgttagggtt ttaaaagttc ttggtgagac taatatcatt ttgggagaga gtattataag tagtcttaag gcattaaaaa ccgattatga acttcttaag atgaaagaag cgcatattat tgatgctatt gcattgatta aatttttacg taaatttaaa agtcttagca aggttgaatt gtctgaatta gatgaaatag atgttgctga tatgcttttg cattttagaa aattgaataa agattttttt agttctagct ttgactcaat agttggtttt agagaaaatg gagctcttcc gcattataag cccaaaagag gtagaaaaat aaataccaat ggtcttcttt tgattgattc tgggggttca tattttggac ttggtacgac agacgttaca agagtttttt taataggtga tgcctctgat gaagaaaagc gcgactatac tttggttctt aaggctttca ttggcctttc ttctttaaag tttccatatg gatcttccgg agcttttctt gatggaattt gtagattacc gcttttaaaa aatgaattga attttattca tggcactggg catggtgttg ggttttttct taatgttcat gaacttccag tttctattag tcctaattct agttatcttt ttaaaggatc tgaggtagtt tcaattgagc ctggtcttta tagaacattt agtcacggaa taagaattga gaatttagtt tttgtaaggc aagcttttac aaatgacttt ggagcttttt tagagtttga gaatttaacc cttgttcctt ttgaaaagga attgatagta aaagaaatgc tctcagaaga tgagttaaat tatatcaatg attatcatga atgtgtattt ttaacattaa aagagcgctt tgataatgaa ggagagttgg aatttttagc aaagctaaca agtaaaatat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BobuA.19108.a.B1.PW37855

PURIFICATION DATe:	2/9/2016
CONCENTRATION:	27mg/ml
OBSERVED MW:	68kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGATA TTAATAAAAG ATTAGCTTTG CTTAGAGATC ATATGAGAGA TAATGGGGTT GATGCTTATT TGGTAGCAGG CTATGATCCT CATTTTAGTG AATATTCTCA TGAAAGATAT AGCACTCGTA AGTTTATTAC AGGTTTTTCT GGTAGCTTTG GTACGGTAAT TATAACATTA TCAAAAGCTG TTCTTTTTAC AGATGGTAGG TATTTTTTAC AGGCCGAGCA AGAGCTTAAG GGAACTGAAA TTGCATTAAT AAAGCTTGGA GTAAAAGGAT CTCCTGATAT TTTTACGTAT ATAAATTTGA ATCTTAAAGA ATCAAAGCTT GGAATTTATT CAGATGAGAT TAGTATAAAG TTTTATAAAG AGTTGTCTGA AAAATGTAAA AATACACATA TCAAGGCTTT AAATCAAGAT CTAATTGATT TAATTTGGAA ATCTAGACCC CAGCTTGAGT TTAGCCATGT ATTTGAATTG GCTGATGCTG AGAAGAATAA CAAAAGAGCT GAAAAAATCA AATCTATTTA TTTGATCTTA GAAAAGAATT TGGCAGATTT TTATGTTATT ACTGCTCTTG ATGAGATTGC TTGGGTGCTG AATTTAAGAG GGGCAGATGT TGAAGAATCA GCATTATTTT ATTCGTTTCT TTTAATATCT AGGAATAAAA ATCGAAAAAA TGTTCTTTTT GTTGACAYAA AAAAACTTGA TTCGGGTGTT AAAGAAGCAC TTGAAATGGA AAATTTTGAA ATAGAGTCTT ACAGCAATTT TTACTGTTTT TTGGACAAAA TAAAGCACGA AGGTAAATTT TTTGTTTCAT TTTATGCCAA TGTTAGGGTT TTAAAAGTTC TTGGTGAGAC TAATATCATT TTGGGAGAGA GTATTATAAG TAGTCTTAAG GCATTAAAAA CCGATTATGA ACTTCTTAAG ATGAAAGAAG CGCATATTAT TGATGCTATT GCATTGATTA AATTTTTACG TAAATTTAAA AGTCTTAGCA AGGTTGAATT GTCTGAATTA GATGAAATAG ATGTTGCTGA TATGCTTTTG CATTTTAGAA AATTGAATAA AGATTTTTTT AGTTCTAGCT TTGACTCAAT AGTTGGTTTT AGAGAAAATG GAGCTCTTCC GCATTATAAG CCCAAAAGAG GTAGAAAAAT AAATACCAAT GGTCTTCTTT TGATTGATTC TGGGGGTTCA TATTTTGGAC TTGGTACGAC AGACGTTACA AGAGTTTTTT TAATAGGTGA TGCCTCTGAT GAAGAAAAGC GCGACTATAC TTTGGTTCTT AAGGCTTTCA TTGGCCTTTC TTCTTTAAAG TTTCCATATG GATCTTCCGG AGCTTTTCTT GATGGAATTT GTAGATTACC GCTTTTAAAA AATGAATTGA ATTTTATTCA TGGCACTGGG CATGGTGTTG GGTTTTTTCT TAATGTTCAT GAACTTCCAG TTTCTATTAG TCCTAATTCT AGTTATCTTT TTAAAGGATC TGAGGTAGTT TCAATTGAGC CTGGTCTTTA TAGAACATTT AGTCACGGAA TAAGAATTGA GAATTTAGTT TTTGTAAGGC AAGCTTTTAC AAATGACTTT GGAGCTTTTT TAGAGTTTGA GAATTTAACC CTTGTTCCTT TTGAAAAGGA ATTGATAGTA AAAGAAATGC TCTCAGAAGA TGAGTTAAAT TATATCAATG ATTATCATGA ATGTGTATTT TTAACATTAA AAGAGCGCTT TGATAATGAA GGAGAGTTGG AATTTTTAGC AAAGCTAACA AGTAAAATAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT