SSGCID: Target Page

BobuA.19107.a

glycyl-tRNAsynthase (glyS) (BB_0371)

CENTER ID:	BobuA.19107.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19107.a.B1.GE39236	full length		1	445

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.766
RefSeq:	NP_212505.1
UniProt:	O51344

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVRMEDIISL AKRKGFVFQS SEVYGGLSGA WDYGPLGVEL KKNIKKEWWK SMVYLHENIV GLDSAIFMRP EIWRASGHVD GFSDSMVDCK DCKSRFRADF IDLSKNCPNC KVGNNFTSPR SFNLMFKTHI GVVEDSSSEV YLRPETAQGI FVNFRNVLDS SRLKIPFGIA QVGKAFRNEI VTKNFIFRTC EFEQMEMQFF VHPKQIDEWF CYWQQNRMNF FIETLKISPD RLRFKAHDST QLAHYAKAAF DIEYEFPFGF QEVEGIHNRG NYDLTQHAKF SNKPKVFEYH DLLTKEKYVP YVIETSAGLT RSVLMTLCDA YSEEELSDGD KRIVLRLHPK LAPYKIAIFP LVKKVELTEI ARRIYMELCD DFHIFYDDSG TIGKRYRRQD EIGTPYCVTI DYNTIEDETV TVRERNSMTQ KRIFINDLYS YIKTEILNYK EDFNK

NT Sequence

atggttagaa tggaagatat tatttctctt gcaaaaagaa aaggatttgt atttcagtct tcagaggttt acgggggcct ttcaggagct tgggattatg gtcctttggg ggttgagctt aaaaagaata taaagaaaga gtggtggaag agcatggtgt acttgcatga aaatattgta ggtttagata gtgctatttt tatgcgccct gaaatttgga gagcatctgg tcatgttgat ggtttttcgg attctatggt tgattgcaaa gattgtaaaa gtagatttag agctgatttt attgatttgt caaaaaattg tccgaattgc aaagttggaa ataattttac ctccccaaga agttttaatt taatgtttaa gacccacatt ggagtagtgg aggatagttc tagtgaagtt tatttaaggc ctgagacagc acaaggaatt tttgttaatt ttagaaatgt tttggattct tcaaggctta agattccttt tgggattgct caggtaggta aagcgtttag aaatgagata gttactaaaa attttatatt tagaacttgt gagtttgagc aaatggaaat gcagtttttt gttcatccca agcaaataga cgagtggttt tgttattggc agcaaaatag aatgaatttt tttatagaaa ctcttaaaat tagtcccgat agattaagat ttaaggcgca tgattcaacg cagcttgctc attatgcaaa agctgcattt gatattgagt atgaatttcc gtttggattt caggaagtag aaggaattca caatagaggt aattatgatt taactcagca cgctaaattt tctaataagc ccaaagtatt tgagtatcat gatttgttga caaaagagaa atatgtgcct tatgttattg agacttctgc tggtcttaca aggtctgttt taatgactct ttgtgatgct tattctgagg aagagctctc agatggagac aagcgtattg ttttacgctt acatcccaag ttggctcctt acaagattgc tatatttcct cttgttaaaa aagttgagct tactgagatt gctagaagga tttatatgga gctttgcgat gattttcata tattttacga tgatagtgga acaataggta aaaggtatag acgtcaagac gaaataggaa ctccttattg cgtaacaata gattacaata cgattgagga tgagacagtt actgttagag aaagaaatag catgactcag aagagaattt ttattaatga tttatattca tacattaaaa cagagatttt aaattacaaa gaggatttta ataaatga

Details for BobuA.19107.a.B1.GE39236

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	53kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMV RMEDIISLAK RKGFVFQSSE VYGGLSGAWD YGPLGVELKK NIKKEWWKSM VYLHENIVGL DSAIFMRPEI WRASGHVDGF SDSMVDCKDC KSRFRADFID LSKNCPNCKV GNNFTSPRSF NLMFKTHIGV VEDSSSEVYL RPETAQGIFV NFRNVLDSSR LKIPFGIAQV GKAFRNEIVT KNFIFRTCEF EQMEMQFFVH PKQIDEWFCY WQQNRMNFFI ETLKISPDRL RFKAHDSTQL AHYAKAAFDI EYEFPFGFQE VEGIHNRGNY DLTQHAKFSN KPKVFEYHDL LTKEKYVPYV IETSAGLTRS VLMTLCDAYS EEELSDGDKR IVLRLHPKLA PYKIAIFPLV KKVELTEIAR RIYMELCDDF HIFYDDSGTI GKRYRRQDEI GTPYCVTIDY NTIEDETVTV RERNSMTQKR IFINDLYSYI KTEIL

Validated NT Sequence

atttaaaatc tctgttttaa tgtatgaata taaatcatta ataaaaattc tcttctgagt catgctattt ctttctctaa cagtaactgt ctcatcctca atcgtattgt aatctattgt tacgcaataa ggagttccta tttcgtcttg acgtctatac cttttaccta ttgttccact atcatcgtaa aatatatgaa aatcatcgca aagctccata taaatccttc tagcaatctc agtaagctca acttttttaa caagaggaaa tatagcaatc ttgtaaggag ccaacttggg atgtaagcgt aaaacaatac gcttgtctcc atctgagagc tcttcctcag aataagcatc acaaagagtc attaaaacag accttgtaag accagcagaa gtctcaataa cataaggcac atatttctct tttgtcaaca aatcatgata ctcaaatact ttgggcttat tagaaaattt agcgtgctga gttaaatcat aattacctct attgtgaatt ccttctactt cctgaaatcc aaacggaaat tcatactcaa tatcaaatgc agcttttgca taatgagcaa gctgcgttga atcatgcgcc ttaaatctta atctatcggg actaatttta agagtttcta taaaaaaatt cattctattt tgctgccaat aacaaaacca ctcgtctatt tgcttgggat gaacaaaaaa ctgcatttcc atttgctcaa actcacaagt tctaaatata aaatttttag taactatctc atttctaaac gctttaccta cctgagcaat cccaaaagga atcttaagcc ttgaagaatc caaaacattt ctaaaattaa caaaaattcc ttgtgctgtc tcaggcctta aataaacttc actagaacta tcctccacta ctccaatgtg ggtcttaaac attaaattaa aacttcttgg ggaggtaaaa ttatttccaa ctttgcaatt cggacaattt tttgacaaat caataaaatc agctctaaat ctacttttac aatctttgca atcaaccata gaatccgaaa aaccatcaac atgaccagat gctctccaaa tttcagggcg cataaaaata gcactatcta aacctacaat attttcatgc aagtacacca tgctcttcca ccactctttc tttatattct ttttaagctc aacccccaaa ggaccataat cccaagctcc tgaaaggccc ccgtaaacct ctgaagactg aaatacaaat ccttttcttt ttgcaagaga aataatatct tccattctaa ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtta gaatggaaga tattatttct cttgcaaaaa gaaaaggatt tgtatttcag tcttcagagg tttacggggg cctttcagga gcttgggatt atggtccttt gggggttgag cttaaaaaga atataaagaa agagtggtgg aagagcatgg tgtacttgca tgaaaatatt gtaggtttag atagtgctat ttttatgcgc cctgaaattt ggagagcatc tggtcatgtt gatggttttt cggattctat ggttgattgc aaagattgta aaagtagatt tagagctgat tttattgatt tgtcaaaaaa ttgtccgaat tgcaaagttg gaaataattt tacctcccca agaagtttta atttaatgtt taagacccac attggagtag tggaggatag ttctagtgaa gtttatttaa ggcctgagac agcacaagga atttttgtta attttagaaa tgttttggat tcttcaaggc ttaagattcc ttttgggatt gctcaggtag gtaaagcgtt tagaaatgag atagttacta aaaattttat atttagaact tgtgagtttg agcaaatgga aatgcagttt tttgttcatc ccaagcaaat agacgagtgg ttttgttatt ggcagcaaaa tagaatgaat ttttttatag aaactcttaa aattagtccc gatagattaa gatttaaggc gcatgattca acgcagcttg ctcattatgc aaaagctgca tttgatattg agtatgaatt tccgtttgga tttcaggaag tagaaggaat tcacaataga ggtaattatg atttaactca gcacgctaaa ttttctaata agcccaaagt atttgagtat catgatttgt tgacaaaaga gaaatatgtg ccttatgtta ttgagacttc tgctggtctt acaaggtctg ttttaatgac tctttgtgat gcttattctg aggaagagct ctcagatgga gacaagcgta ttgttttacg cttacatccc aagttggctc cttacaagat tgctatattt cctcttgtta aaaaagttga gcttactgag attgctagaa ggatttatat ggagctttgc gatgattttc atatatttta cgatgatagt ggaacaatag gtaaaaggta tagacgtcaa gacgaaatag gaactcctta ttgcgtaaca atagattaca atacgattga ggatgagaca gttactgtta gagaaagaaa tagcatgact cagaagagaa tttttattaa tgatttatat tcatacatta aaacagagat tttaaattac aaagaggatt ttaataaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat