SSGCID: Target Page

BobuA.19099.a

PTS system, glu-specific IIA (crr) (BB_0559)

CENTER ID:	BobuA.19099.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19099.a.B1.GE39467	full length		1	189

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.950
RefSeq:	NP_212693.1
UniProt:	Q44840

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGFLDFFKKT ATLDLIAPIS GKVMSIDKVP DEAFAEKIVG DGIAILPTSN ELLAPCDGKI GKIFKTNHAF SLETKEGVEI FVHFGINTLN LNGKGFTRVA EEGINVKQGE VIIRLDLEYL KEHSESVITP VVIANSDEVS SIEYSFGRLE NDSEYILSSS TVLTEEIRHK ISQTKPVIAG KDLVLRVKK

NT Sequence

atggggtttt tagatttttt taaaaaaacc gctacattgg atttgattgc tccgattagt ggaaaagtta tgtcaattga taaggttccc gatgaagctt ttgctgaaaa aatagttggc gatggaattg caattcttcc aacaagcaat gagttgttgg cgccttgtga tgggaaaata ggtaaaattt ttaaaaccaa tcatgccttt agccttgaaa ctaaagaggg cgttgaaatt tttgtccatt ttggaattaa tactcttaat ttaaatggta agggttttac aagagttgct gaagagggca ttaatgttaa acaaggtgaa gttattatta ggcttgatct tgaatattta aaagagcatt cagaatccgt tattactccg gttgttattg caaattctga tgaagtttca agtatagaat attcttttgg aaggcttgaa aatgattctg aatatatttt atcatcttca actgtcttga cagaagaaat taggcataaa atatctcaaa caaagcctgt tatagcgggc aaagatttgg tgttgcgagt taaaaagtaa

Details for BobuA.19099.a.B1.GE39467

HARVESTED ON:	data unavailable
SEQUENCED ON:	9/16/2015
EXPECTED MW:	22kDa
OBSERVED MW:	data unavailable
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Data Unavailable
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG FLDFFKKTAT LDLIAPISGK VMSIDKVPDE AFAEKIVGDG IAILPTSNEL LAPCDGKIGK IFKTNHAFSL ETKEGVEIFV HFGINTLNLN GKGFTRVAEE GINVKQGEVI IRLDLEYLKE HSESVITPVV IANSDEVSSI EYSFGRLEND SEYILSSSTV LTEEIRHKIS QTKPVIAGKD LVLRVKK

Validated NT Sequence

atcttactca cttacttttt aactcgcaac accaaatctt tgcccgctat aacaggcttt gtttgagata ttttatgcct aatttcttct gtcaagacag ttgaagatga taaaatatat tcagaatcat tttcaagcct tccaaaagaa tattctatac ttgaaacttc atcagaattt gcaataacaa ccggagtaat aacggattct gaatgctctt ttaaatattc aagatcaagc ctaataataa cttcaccttg tttaacatta atgccctctt cagcaactct tgtaaaaccc ttaccattta aattaagagt attaattcca aaatggacaa aaatttcaac gccctcttta gtttcaaggc taaaggcatg attggtttta aaaattttac ctattttccc atcacaaggc gccaacaact cattgcttgt tggaagaatt gcaattccat cgccaactat tttttcagca aaagcttcat cgggaacctt atcaattgac ataacttttc cactaatcgg agcaatcaaa tccaatgtag cggttttttt aaaaaaatct aaaaacccca tatggtggtg gtggtggtga gccatggt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggt ttttagattt ttttaaaaaa accgctacat tggatttgat tgctccgatt agtggaaaag ttatgtcaat tgataaggtt cccgatgaag cttttgctga aaaaatagtt ggcgatggaa ttgcaattct tccaacaagc aatgagttgt tggcgccttg tgatgggaaa ataggtaaaa tttttaaaac caatcatgcc tttagccttg aaactaaaga gggcgttgaa atttttgtcc attttggaat taatactctt aatttaaatg gtaagggttt tacaagagtt gctgaagagg gcattaatgt taaacaaggt gaagttatta ttaggcttga tcttgaatat ttaaaagagc attcagaatc cgttattact ccggttgtta ttgcaaattc tgatgaagtt tcaagtatag aatattcttt tggaaggctt gaaaatgatt ctgaatatat tttatcatct tcaactgtct tgacagaaga aattaggcat aaaatatctc aaacaaagcc tgttatagcg ggcaaagatt tggtgttgcg agttaaaaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat