BobuA.19089.a

Uncharacterized protein (BB_0418)

CENTER ID: BobuA.19089.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.19089.a.B2.GE39462 mature protein 20 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.812
RefSeq: NP_212552.1
UniProt: O51379

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLIKKICFCL LLLQAKIILS QDQQNLEFNN EYFYFNFQSA YYPPHELGTN GSGFSQSFKH PNFQNIDPSL KIPVNYWGGI KLISYLGYYK NFKALNNPNS IFFKNNGIDI DLNIGLSPVI VSFKSTVSFT PIAFLNLYAS NELGIGWQAF GFKGVGVHIE NGKYSNAVEF YSEITAGGRF QFDLNAIFQG EWTHIITVIG NDFTYLINPH AKKDQLWKYK ADDGKNINGM LIKPYALLAY KMPIPLNTIG LLYEGKTQIG KERNISSWKN KGWGSDIFYH NISIVANFEI IKPLTLGLQF KLSTNPTYTS NTAGLADISK RIATGNSYFY YDSIGMSITY KY
NT Sequence
atgttaataa aaaaaatttg cttttgtttg ttacttttgc aagcaaaaat aattctttcc caagatcaac agaacttaga atttaataat gaatattttt attttaattt tcaatctgct tattatcctc cccacgagct gggtacaaat ggaagcggct tttcacaaag ctttaaacat cctaacttcc aaaatattga cccaagttta aaaattccag tcaattattg ggggggaata aaactaatat catatttggg ctactataaa aattttaaag ccctaaacaa tcccaattct atatttttta aaaataatgg tatagatata gatttaaata tcggattgtc tcctgtaatt gtctcattta aaagcacagt aagctttact cctattgctt ttttaaattt atatgcatca aatgaacttg gcataggttg gcaagctttt ggatttaaag gagtaggcgt acacattgaa aatgggaaat attcaaatgc tgttgaattt tattcagaaa taacagcggg gggacgattc caatttgact taaacgctat ttttcaagga gaatggacac acattattac agttattgga aatgatttta catacttaat caatccccat gcaaaaaaag atcaactttg gaaatataaa gcagatgatg gtaaaaatat aaatggcatg ctaataaaac cttacgctct tttggcttac aaaatgccaa tacctcttaa caccataggc ctgctttatg aaggaaaaac acaaattggc aaagaaagaa acataagctc ttggaaaaac aaaggctggg gaagtgatat tttttatcac aacatttcta ttgttgctaa ttttgaaatt atcaaacctt taacacttgg ccttcaattt aaattatcta caaatcccac atacacaagt aacacagccg gactggctga tatttcaaaa agaatagcaa ctggcaattc ttatttttat tacgattcta ttggaatgtc tataacttat aaatactaa
Details for BobuA.19089.a.B2.GE39462
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 38kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS QDQQNLEFNN EYFYFNFQSA YYPPHELGTN GSGFSQSFKH PNFQNIDPSL KIPVNYWGGI KLISYLGYYK NFKALNNPNS IFFKNNGIDI DLNIGLSPVI VSFKSTVSFT PIAFLNLYAS NELGIGWQAF GFKGVGVHIE NGKYSNAVEF YSEITAGGRF QFDLNAIFQG EWTHIITVIG NDFTYLINPH AKKDQLWKYK ADDGKNINGM LIKPYALLAY KMPIPLNTIG LLYEGKTQIG KERNISSWKN KGWGSDIFYH NISIVANFEI IKPLTLGLQF KLSTNPTYTS NTAGLADISK RIATGNSYFY YDSIGMSITY KY
Validated NT Sequence
ttactcactt agtatttata agttatagac attccaatag aatcgtaata aaaataagaa ttgccagttg ctattctttt tgaaatatca gccagtccgg ctgtgttact tgtgtatgtg ggatttgtag ataatttaaa ttgaaggcca agtgttaaag gtttgataat ttcaaaatta gcaacaatag aaatgttgtg ataaaaaata tcacttcccc agcctttgtt tttccaagag cttatgtttc tttctttgcc aatttgtgtt tttccttcat aaagcaggcc tatggtgtta agaggtattg gcattttgta agccaaaaga gcgtaaggtt ttattagcat gccatttata tttttaccat catctgcttt atatttccaa agttgatctt tttttgcatg gggattgatt aagtatgtaa aatcatttcc aataactgta ataatgtgtg tccattctcc ttgaaaaata gcgtttaagt caaattggaa tcgtcccccc gctgttattt ctgaataaaa ttcaacagca tttgaatatt tcccattttc aatgtgtacg cctactcctt taaatccaaa agcttgccaa cctatgccaa gttcatttga tgcatataaa tttaaaaaag caataggagt aaagcttact gtgcttttaa atgagacaat tacaggagac aatccgatat ttaaatctat atctatacca ttatttttaa aaaatataga attgggattg tttagggctt taaaattttt atagtagccc aaatatgata ttagttttat tcccccccaa taattgactg gaatttttaa acttgggtca atattttgga agttaggatg tttaaagctt tgtgaaaagc cgcttccatt tgtacccagc tcgtggggag gataataagc agattgaaaa ttaaaataaa aatattcatt attaaattct aagttctgtt gatcttggga catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHSQ DQQNLEFNNE YFYFNFQSAY YPPHELGTNG SGFSQSFKHP NFQNIDPSLK IPVNYWGGIK LISYLGYYKN FKALNNPNSI FFKNNGIDID LNIGLSPVIV SFKSTVSFTP IAFLNLYASN ELGIGWQAFG FKGVGVHIEN GKYSNAVEFY SEITAGGRFQ FDLNAIFQGE WTHIITVIGN DFTYLINPHA KKDQLWKYKA DDGKNINGML IKPYALLAYK MPIPLNTIGL LYEGKTQIGK ERNISSWKNK GWGSDIFYHN ISIVANFEII KPLTLGLQFK LSTNPTYTSN TAGLADISKR IATGNSYFYY DSIGMSITYK Y
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcccaag atcaacagaa cttagaattt aataatgaat atttttattt taattttcaa tctgcttatt atcctcccca cgagctgggt acaaatggaa gcggcttttc acaaagcttt aaacatccta acttccaaaa tattgaccca agtttaaaaa ttccagtcaa ttattggggg ggaataaaac taatatcata tttgggctac tataaaaatt ttaaagccct aaacaatccc aattctatat tttttaaaaa taatggtata gatatagatt taaatatcgg attgtctcct gtaattgtct catttaaaag cacagtaagc tttactccta ttgctttttt aaatttatat gcatcaaatg aacttggcat aggttggcaa gcttttggat ttaaaggagt aggcgtacac attgaaaatg ggaaatattc aaatgctgtt gaattttatt cagaaataac agcgggggga cgattccaat ttgacttaaa cgctattttt caaggagaat ggacacacat tattacagtt attggaaatg attttacata cttaatcaat ccccatgcaa aaaaagatca actttggaaa tataaagcag atgatggtaa aaatataaat ggcatgctaa taaaacctta cgctcttttg gcttacaaaa tgccaatacc tcttaacacc ataggcctgc tttatgaagg aaaaacacaa attggcaaag aaagaaacat aagctcttgg aaaaacaaag gctggggaag tgatattttt tatcacaaca tttctattgt tgctaatttt gaaattatca aacctttaac acttggcctt caatttaaat tatctacaaa tcccacatac acaagtaaca cagccggact ggctgatatt tcaaaaagaa tagcaactgg caattcttat ttttattacg attctattgg aatgtctata acttataaat actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat