BobuA.19088.a

Uncharacterized protein (BB_0707)

CENTER ID: BobuA.19088.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.19088.a.B1.GE39461 full length 1 590
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1098
RefSeq: NP_212841.2
UniProt: O51650

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGNNNSVYN SIGQLSKETK DRLMNDIRKS LGLNSSDFVL PNSSNEPYVD SQLEKFLSGR LMEESFLIRM WLTILNFFQK DKSKEDIYKA YVIKNLENQI NEMCKNPVID FKRECLHVGF VEMFFSVCRY SMELKEFFRK LEKGGIVVEQ TILEIIQSKV LNSKNNLEDF LDEGEYELFL KKEKTQNDLE ESLKVKINEY INSIPSSTYK IVSDMFEFYY VFNSLAFFPY KSFFSFFNVD LLDSAENISI VDFEVGFGTS FSSVSKYLNS FFDILYTLKD IEIDEDIVKS IISNYFSISD DISNSEIISR EDHISRVEYV FKNVLSIVSK IVGLSRTLPY LDIFRVYCKN PVASPKKYVP FFDVKNFYEN ILFLNVMGQA IKKRDSSLKV LVIKEIKNLV RDPSLILNLK GEIFNELNLG RYSFKKLYFL NDFFKNIYDV RMMEVLRTIN NVVLINNHDL RNIYVDIENN ITALKKEIYN IYFEIDYKNE EFERYKRDGK IDNSYKERIL KWCLTESVSI DKVSSRFVDY FIELKKRCLS LLENHNVFIR KSLTVSYKSM VTENKTITLA DVIGNLLEII NQALFIIKNL
NT Sequence
atgagtggta ataacaatag cgtatacaac tctataggtc aactttcaaa agagactaaa gataggttga tgaatgatat aagaaaaagc ttaggtttga attcttccga ttttgttttg cccaatagta gtaatgagcc ttatgttgat tctcagcttg aaaaatttct ttcggggcgc ttaatggaag aatcatttct tattagaatg tggttaacta ttttaaattt ttttcagaaa gataaaagca aagaagatat atataaagct tatgttataa aaaacctgga aaatcaaatt aatgaaatgt gtaaaaatcc agtaatagat tttaagagag aatgtttgca tgttggtttt gtggaaatgt tttttagtgt ttgccgatat tcaatggaat tgaaagaatt ttttaggaaa ttagaaaaag gtggtattgt tgttgagcaa actattttag aaattattca aagcaaagtt cttaactcta agaacaattt ggaagatttt ttagatgaag gtgaatatga gcttttttta aaaaaagaaa aaacccaaaa cgatttagaa gaatctctta aggtcaagat aaatgaatat attaattcta ttccatctag tacttacaaa atcgtctcgg atatgtttga gttttattat gtttttaata gtttggcgtt tttcccttac aaatcttttt tttcattttt taatgtagat cttttagata gtgctgagaa tattagcatt gttgactttg aggttggatt tggaaccagc ttttcaagtg ttagtaagta tttgaattct ttttttgaca ttttgtatac attaaaagat attgagatag atgaggatat tgttaaaagc attatatcga attatttttc aatatctgat gatattagta attcagagat aatttcaaga gaagatcata tatctcgggt agaatatgta tttaaaaatg ttttatctat tgtatctaag attgttggct tgtcgaggac tttgccttat ttagatattt ttagagtata ttgcaaaaat cctgttgcat ctcctaaaaa atatgtaccc ttttttgatg taaaaaattt ttatgagaac attttatttt taaatgttat gggtcaggct attaaaaagc gtgatagctc tttaaaagtt cttgttatca aagaaataaa aaatcttgtt agagatccta gtttaatttt aaatttaaaa ggcgagattt ttaatgaatt aaatcttggg cgttatagtt ttaaaaagtt atattttctt aatgattttt ttaaaaacat atatgatgtt agaatgatgg aagttttaag aactataaat aatgtcgtat taatcaataa tcatgatttg agaaatatat atgttgacat tgaaaataat attactgctt tgaaaaaaga aatttataat atttactttg agattgatta taaaaatgaa gagtttgaaa ggtataaaag agacggtaaa attgataact cttataagga aaggattttg aagtggtgct taactgaatc cgtttctatt gataaggttt caagtagatt tgtggattat tttattgagc ttaaaaagag gtgcttgtct ttgttggaaa atcataatgt ttttattcga aaatctttaa cggtttctta caaatcaatg gtaactgaaa ataaaacaat tacattagcc gatgttattg gcaatctttt ggaaatcata aatcaagcac tttttataat aaagaattta taa
Details for BobuA.19088.a.B1.GE39461
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 93
PERCENT COVERAGE: 51
Validated AA Sequence
MAHHHHHHMS GNNNSVYNSI GQLSKETKDR LMNDIRKSLG LNSSDFVLPN SSNEPYVDSQ LEKFLSGRLM EESFLIRMWL TILNFFQKDK SKEDIYKAYV IKNLENQINE MCKNPVIDFK RECLHVGFVE MFFSVCRYSM ELKEFFRKLE KGGIVVEQTI LEIIQSKVLN SKNNLEDFLD EGEYELFLKK EKTQNDLEES LKVKINEYIN SIPSSTYKIV SDMFEFYYVF NSLAFFPYKS FFSFFNVDLL XSAENISIVD FEVGFGTSFS SVSKYLNSXX DILYTXXXXX XXXDIXXXXI SXXXXNI
Validated NT Sequence
gcttgattta tgatttccaa aagattgcca ataacatcgg ctaatgtaat tgttttattt tcagttacca ttgatttgta agaaaccgtt aaagattttc gaataaaaac attatgattt tccaacaaag acaagcacct ctttttaagc tcaataaaat aatccacaaa tctacttgaa accttatcaa tagaaacgga ttcagttaag caccacttca aaatcctttc cttataagag ttatcaattt taccgtctct tttatacctt tcaaactctt catttttata atcaatctca aagtaaatat tataaatttc ttttttcaaa gcagtaatat tattttcaat gtcaacatat atatttctca aatcatgatt attgattaat acgacattat ttatagttct taaaacttcc atcattctaa catcatatat gtttttaaaa aaatcattaa gaaaatataa ctttttaaaa ctataacgcc caagatttaa ttcattaaaa atctcgcctt ttaaatttaa aattaaacta ggatctctaa caagattttt tatttctttg ataacaagaa cttttaaaga gctatcacgc tttttaatag cctgacccat aacatttaaa aataaaatgt tctcataaaa attttttaca tcaaaaaagg gtacatattt tttaggagat gcaacaggat ttttgcaata tactctaaaa atatctaaat aaggcaaagt cctcgacaag ccaacaatct tanntacnat agataaaaca tttttaaata catattctac cngagatata tgatcttctc ttgaaattat ctctnnnnnn ctaatatcat cagatattga naannnnnnn ngatataann nnnnnnnnaa tatcnnnnnn nnnnncaann nnnnnnnntg tatacaaaat gtcannnaan gaattcaaat acttactaac acttgaaaag ctggttccaa atccaacctc aaagtcaaca atgctaatat tctcagcact anctaaaaga tctacattaa aaaatgaaaa aaaagatttg taagggaaaa acgccaaact attaaaaaca taataaaact caaacatatc cgagacgatt ttgtaagtac tagatggaat agaattaata tattcattta tcttgacctt aagagattct tctaaatcgt tttgggtttt ttcttttttt aaaaaaagct catattcacc ttcatctaaa aaatcttcca aattgttctt agagttaaga actttgcttt gaataatttc taaaatagtt tgctcaacaa caataccacc tttttctaat ttcctaaaaa attctttcaa ttccattgaa tatcggcaaa cactaaaaaa catttccaca aaaccaacat gcaaacattc tctcttaaaa tctattactg gatttttaca catttcatta atttgatttt ccaggttttt tataacataa gctttatata tatcttcttt gcttttatct ttctgaaaaa aatttaaaat agttaaccac attctaataa gaaatgattc ttccattaag cgccccgaaa gaaatttttc aagctgagaa tcaacataag gctcattact actattgggc aaaacaaaat cggaagaatt caaacctaag ctttttctta tatcattcat caacctatct ttagtctctt ttgaaagttg acctatagag ttgtatacgc tattgttatt accactcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS GNNNSVYNSI GQLSKETKDR LMNDIRKSLG LNSSDFVLPN SSNEPYVDSQ LEKFLSGRLM EESFLIRMWL TILNFFQKDK SKEDIYKAYV IKNLENQINE MCKNPVIDFK RECLHVGFVE MFFSVCRYSM ELKEFFRKLE KGGIVVEQTI LEIIQSKVLN SKNNLEDFLD EGEYELFLKK EKTQNDLEES LKVKINEYIN SIPSSTYKIV SDMFEFYYVF NSLAFFPYKS FFSFFNVDLL DSAENISIVD FEVGFGTSFS SVSKYLNSFF DILYTLKDIE IDEDIVKSII SNYFSISDDI SNSEIISRED HISRVEYVFK NVLSIVSKIV GLSRTLPYLD IFRVYCKNPV ASPKKYVPFF DVKNFYENIL FLNVMGQAIK KRDSSLKVLV IKEIKNLVRD PSLILNLKGE IFNELNLGRY SFKKLYFLND FFKNIYDVRM MEVLRTINNV VLINNHDLRN IYVDIENNIT ALKKEIYNIY FEIDYKNEEF ERYKRDGKID NSYKERILKW CLTESVSIDK VSSRFVDYFI ELKKRCLSLL ENHNVFIRKS LTVSYKSMVT ENKTITLADV IGNLLEIINQ ALFIIKNL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg gtaataacaa tagcgtatac aactctatag gtcaactttc aaaagagact aaagataggt tgatgaatga tataagaaaa agcttaggtt tgaattcttc cgattttgtt ttgcccaata gtagtaatga gccttatgtt gattctcagc ttgaaaaatt tctttcgggg cgcttaatgg aagaatcatt tcttattaga atgtggttaa ctattttaaa tttttttcag aaagataaaa gcaaagaaga tatatataaa gcttatgtta taaaaaacct ggaaaatcaa attaatgaaa tgtgtaaaaa tccagtaata gattttaaga gagaatgttt gcatgttggt tttgtggaaa tgttttttag tgtttgccga tattcaatgg aattgaaaga attttttagg aaattagaaa aaggtggtat tgttgttgag caaactattt tagaaattat tcaaagcaaa gttcttaact ctaagaacaa tttggaagat tttttagatg aaggtgaata tgagcttttt ttaaaaaaag aaaaaaccca aaacgattta gaagaatctc ttaaggtcaa gataaatgaa tatattaatt ctattccatc tagtacttac aaaatcgtct cggatatgtt tgagttttat tatgttttta atagtttggc gtttttccct tacaaatctt ttttttcatt ttttaatgta gatcttttag atagtgctga gaatattagc attgttgact ttgaggttgg atttggaacc agcttttcaa gtgttagtaa gtatttgaat tctttttttg acattttgta tacattaaaa gatattgaga tagatgagga tattgttaaa agcattatat cgaattattt ttcaatatct gatgatatta gtaattcaga gataatttca agagaagatc atatatctcg ggtagaatat gtatttaaaa atgttttatc tattgtatct aagattgttg gcttgtcgag gactttgcct tatttagata tttttagagt atattgcaaa aatcctgttg catctcctaa aaaatatgta cccttttttg atgtaaaaaa tttttatgag aacattttat ttttaaatgt tatgggtcag gctattaaaa agcgtgatag ctctttaaaa gttcttgtta tcaaagaaat aaaaaatctt gttagagatc ctagtttaat tttaaattta aaaggcgaga tttttaatga attaaatctt gggcgttata gttttaaaaa gttatatttt cttaatgatt tttttaaaaa catatatgat gttagaatga tggaagtttt aagaactata aataatgtcg tattaatcaa taatcatgat ttgagaaata tatatgttga cattgaaaat aatattactg ctttgaaaaa agaaatttat aatatttact ttgagattga ttataaaaat gaagagtttg aaaggtataa aagagacggt aaaattgata actcttataa ggaaaggatt ttgaagtggt gcttaactga atccgtttct attgataagg tttcaagtag atttgtggat tattttattg agcttaaaaa gaggtgcttg tctttgttgg aaaatcataa tgtttttatt cgaaaatctt taacggtttc ttacaaatca atggtaactg aaaataaaac aattacatta gccgatgtta ttggcaatct tttggaaatc ataaatcaag cactttttat aataaagaat ttatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat