SSGCID: Target Page

BobuA.19087.a

spoIIIJ-associated protein (jag) (BB_0443)

CENTER ID:	BobuA.19087.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.19087.a.B1.GE39460	full length		1	242

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.834
RefSeq:	NP_212577.1
RefSeq:	WP_010889751.1
UniProt:	O51399

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYEFYGKTE QEAIKKAMRD LELKEGEFDV EILDKERVGF LFKKEMIKIR VSPHAKEVKK DDFEIKIGDE ICDKILEFVK EMLIKMGYSV HLTIEPKEGG YVKISIETDS PNILIGREGK NLDSLQLLTN VYASKLIGEN GAFNRVILDI GDYRERFKSR FINLAINSFH KVKRTRRSIL LPSMNPFERR IVHTTLNRYS DIKTESEGDG NIKRIRVSYV RNNRYGINNS RGYQKRDIGF KK

NT Sequence

atgagctatg aattttacgg aaaaaccgag caagaagcaa taaagaaagc aatgagagat ctagaattaa aagaaggcga gtttgatgta gaaattttag ataaagaaag ggttggattt ttatttaaga aagaaatgat taaaatacgg gtttctcctc atgcaaagga ggttaaaaaa gacgattttg aaattaaaat tggtgatgaa atttgtgata aaattttaga atttgtaaaa gaaatgctaa ttaaaatggg atattctgta catttgacaa tagagcccaa agaaggggga tatgttaaga tttctattga aacagatagt ccaaatattt tgattggacg agagggtaaa aatttagatt ctttgcagct tttaacaaat gtttatgcct ctaagcttat tggtgaaaat ggggctttta atagggttat attggatatt ggagattata gagaacggtt taaatctagg tttattaatt tagcaataaa ttcttttcat aaagtcaaaa gaaccaggcg ctctattttg ttgccatcaa tgaatccttt tgaaagaagg attgttcata caactttaaa tcgttatagt gacataaaaa ctgaaagtga gggagatgga aatataaaaa gaataagagt ctcttatgtt agaaataata ggtatggtat caacaattct cgtggttatc agaaaagaga tattggtttt aaaaaatag

Details for BobuA.19087.a.B1.GE39460

HARVESTED ON:	9/10/2015
SEQUENCED ON:	9/16/2015
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS YEFYGKTEQE AIKKAMRDLE LKEGEFDVEI LDKERVGFLF KKEMIKIRVS PHAKEVKKDD FEIKIGDEIC DKILEFVKEM LIKMGYSVHL TIEPKEGGYV KISIETDSPN ILIGREGKNL DSLQLLTNVY ASKLIGENGA FNRVILDIGD YRERFKSRFI NLAINSFHKV KRTRRSILLP SMNPFERRIV HTTLNRYSDI KTESEGDGNI KRIRVSYVRN NRYGINNSRG YQKRDIGLKN K

Validated NT Sequence

atcttactca cttatttttt aaaccaatat ctcttttctg ataaccacga gaattgttga taccatacct attatttcta acataagaga ctcttattct ttttatattt ccatctccct cactttcagt ttttatgtca ctataacgat ttaaagttgt atgaacaatc cttctttcaa aaggattcat tgatggcaac aaaatagagc gcctggttct tttgacttta tgaaaagaat ttattgctaa attaataaac ctagatttaa accgttctct ataatctcca atatccaata taaccctatt aaaagcccca ttttcaccaa taagcttaga ggcataaaca tttgttaaaa gctgcaaaga atctaaattt ttaccctctc gtccaatcaa aatatttgga ctatctgttt caatagaaat cttaacatat cccccttctt tgggctctat tgtcaaatgt acagaatatc ccattttaat tagcatttct tttacaaatt ctaaaatttt atcacaaatt tcatcaccaa ttttaatttc aaaatcgtct tttttaacct cctttgcatg aggagaaacc cgtattttaa tcatttcttt cttaaataaa aatccaaccc tttctttatc taaaatttct acatcaaact cgccttcttt taattctaga tctctcattg ctttctttat tgcttcttgc tcggtttttc cgtaaaattc atagctcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct atgaatttta cggaaaaacc gagcaagaag caataaagaa agcaatgaga gatctagaat taaaagaagg cgagtttgat gtagaaattt tagataaaga aagggttgga tttttattta agaaagaaat gattaaaata cgggtttctc ctcatgcaaa ggaggttaaa aaagacgatt ttgaaattaa aattggtgat gaaatttgtg ataaaatttt agaatttgta aaagaaatgc taattaaaat gggatattct gtacatttga caatagagcc caaagaaggg ggatatgtta agatttctat tgaaacagat agtccaaata ttttgattgg acgagagggt aaaaatttag attctttgca gcttttaaca aatgtttatg cctctaagct tattggtgaa aatggggctt ttaatagggt tatattggat attggagatt atagagaacg gtttaaatct aggtttatta atttagcaat aaattctttt cataaagtca aaagaaccag gcgctctatt ttgttgccat caatgaatcc ttttgaaaga aggattgttc atacaacttt aaatcgttat agtgacataa aaactgaaag tgagggagat ggaaatataa aaagaataag agtctcttat gttagaaata ataggtatgg tatcaacaat tctcgtggtt atcagaaaag agatattggt tttaaaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat