BobuA.19051.a

melvalonate kinase (BB_0688)

CENTER ID: BobuA.19051.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.19051.a.B2.GE39234 soluble domain 40 297
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1079
RefSeq: NP_212822.1
UniProt: O51631

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRIRKPAKI LFLGEHSAVY GFPVIGATVP IYMDLIYSVS KNWKYLGKPS TRLNSLISFI VSNYSKVNPI EFDIISEIPI GVGLGSSASL SLCFAEYITS HFEYKDCNKI LLANQIENIF HGKSSGMDIR LIDLNGTFYL EKKENVLHSK KIKDSGFYFL IGAIKRDLTT KEIVVNLKKD LLSNAYLFVF IEKLGLAVSN SYASFQNKDV YSLANEMNIA QCCLKRLGLS NDTLDWLISE GIKLGALSGK LSGAGKGGAF IFLFESLIKA NIVQKELNNM LKDSKIDLLL KLKVIET
NT Sequence
atgctaagaa taagaaagcc tgctaaaata ttgttcttag gcgaacatag cgctgtttat gggttcccag ttattggcgc tacagttcca atttatatgg atctgattta tagtgtatct aaaaattgga aatatttagg aaaacccagt acaagattaa atagccttat aagttttatt gtttcaaatt atagcaaagt taatcctatt gagtttgata taatttctga aattcctatt ggagttggtc ttggttcttc tgctagtctt agtttatgct ttgcagaata tattacaagt cattttgaat ataaggattg taataaaatt ttgttggcaa atcaaattga aaacattttt catggcaaat cttctggaat ggatattaga ctaatcgatc ttaatggaac tttttattta gagaaaaaag aaaatgtttt acattcaaag aaaataaaag attctggttt ttattttcta ataggagcaa taaaaagaga tttaacaact aaagaaatag ttgttaattt gaaaaaagat ctattatcaa atgcttattt atttgttttt attgaaaagc ttggccttgc cgtaagcaat tcttatgctt cttttcagaa taaggatgtg tattctttag ctaatgaaat gaatattgca caatgttgtt taaagcgttt ggggttgtct aatgatactc ttgattggct gataagtgag ggaatcaaat tgggcgctct ttctggtaag ttaagtggcg ctggtaaagg aggagcattt atttttcttt ttgaaagcct aataaaagct aatatagtgc aaaaagaatt aaataacatg cttaaagatt ctaaaataga tttgctttta aagctaaagg taattgagac ttag
Details for BobuA.19051.a.B2.GE39234
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS KNWKYLGKPS TRLNSLISFI VSNYSKVNPI EFDIISEIPI GVGLGSSASL SLCFAEYITS HFEYKDCNKI LLANQIENIF HGKSSGMDIR LIDLNGTFYL EKKENVLHSK KIKDYGFYFL IGAIKRDLTT KEIVVNLKED LLSNAYLFVF IEKLGLAVSN SYASFQNKDV YSLANEMNIA QCCLKRLGLS NDTLDWLISE GIKLGALSGK LSGAGKGGAF IFLFESLIKA YIVQKELNNM LKDSKIDLLL KLKVIET
Validated NT Sequence
atcttactca cttaagtctc aattaccttt agctttaaaa gcaaatctat tttagaatct ttaagcatgt tatttaattc tttttgcact atataagctt ttattaggct ttcaaaaaga aaaataaatg ctcctccttt accagcgcca cttaacttac cagaaagagc gcccaatttg attccctcac ttatcagcca atcaagagta tcattagaca accccaaacg ctttaaacaa cattgtgcaa tattcatttc attagctaaa gaatacacat ccttattctg aaaagaagca taagaattgc ttacggcaag gccaagcttt tcaataaaaa caaataaata agcatttgat aatagatctt ctttcaaatt aacaactatt tctttagttg ttaaatctct ttttattgct cctattagaa aataaaaacc ataatctttt attttctttg aatgtaaaac attttctttt ttctctaaat aaaaagttcc attaagatcg attagtctaa tatccattcc agaagatttg ccatgaaaaa tgttttcaat ttgatttgcc aacaaaattt tattacaatc cttatattca aaatgacttg taatatattc tgcaaagcat aaactaagac tagcagaaga accaagacca actccaatag gaatttcaga aattatatca aactcaatag gattaacttt gctataattt gaaacaataa aacttataag gctatttaat cttgtactgg gttttcctaa atatttccaa tttttagaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHSK NWKYLGKPST RLNSLISFIV SNYSKVNPIE FDIISEIPIG VGLGSSASLS LCFAEYITSH FEYKDCNKIL LANQIENIFH GKSSGMDIRL IDLNGTFYLE KKENVLHSKK IKDSGFYFLI GAIKRDLTTK EIVVNLKKDL LSNAYLFVFI EKLGLAVSNS YASFQNKDVY SLANEMNIAQ CCLKRLGLSN DTLDWLISEG IKLGALSGKL SGAGKGGAFI FLFESLIKAN IVQKELNNML KDSKIDLLLK LKVIET
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctaaaa attggaaata tttaggaaaa cccagtacaa gattaaatag ccttataagt tttattgttt caaattatag caaagttaat cctattgagt ttgatataat ttctgaaatt cctattggag ttggtcttgg ttcttctgct agtcttagtt tatgctttgc agaatatatt acaagtcatt ttgaatataa ggattgtaat aaaattttgt tggcaaatca aattgaaaac atttttcatg gcaaatcttc tggaatggat attagactaa tcgatcttaa tggaactttt tatttagaga aaaaagaaaa tgttttacat tcaaagaaaa taaaagattc tggtttttat tttctaatag gagcaataaa aagagattta acaactaaag aaatagttgt taatttgaaa aaagatctat tatcaaatgc ttatttattt gtttttattg aaaagcttgg ccttgccgta agcaattctt atgcttcttt tcagaataag gatgtgtatt ctttagctaa tgaaatgaat attgcacaat gttgtttaaa gcgtttgggg ttgtctaatg atactcttga ttggctgata agtgagggaa tcaaattggg cgctctttct ggtaagttaa gtggcgctgg taaaggagga gcatttattt ttctttttga aagcctaata aaagctaata tagtgcaaaa agaattaaat aacatgctta aagattctaa aatagatttg cttttaaagc taaaggtaat tgagacttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at