BobuA.19042.a

basic membrane protein D (bmpD) (BB_0385)

CENTER ID: BobuA.19042.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.19042.a.B2.GE39310 mature protein 20 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.780
RefSeq: NP_212519.2
UniProt: P0CL55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKKVYYFLI FLFIVACSSS DDGKSEAKTV SLIVDGAFDD KGFNESSSKA IRKLKADLNI NIIEKASTGN SYLGDIANLE DGNSNLIWGI GFRLSDILFQ RASENVSVNY AIIEGVYDEI QIPKNLLNIS FRSEEVAFLA GYFASKASKT GKIGFVGGVR GKVLESFMYG YEAGAKYANS NIKVVSQYVG TFGDFGLGRS TASNMYRDGV DIIFAAAGLS GIGVIEAAKE LGPDHYIIGV DQDQSYLAPN NVIVSAVKKV DSLMYSLTKK YLETGVLDGG KTMFLGLKED GLGLVLNENL KSNYSEIYNK SLKIGQSIMN GIIKVPYDKV SYDNFVLQME N
NT Sequence
atgttaaaaa aagtttatta ttttttaatt tttttattta ttgttgcttg ttctagctct gatgatggca agtcggaggc aaaaacagtt tcgcttatag ttgatggtgc ttttgatgat aaaggattta atgaaagttc ttctaaggcg ataagaaaat taaaggcaga tttaaatata aatataattg aaaaagcatc tacaggcaat tcttatttag gagatattgc aaacttagaa gatggtaatt caaatttgat ttggggaatt gggtttagat tgtcagacat tctttttcaa agagctagcg agaatgtttc tgttaattat gcaatcatag aaggggttta tgatgaaatt caaataccca aaaatcttct taatattagt tttagatccg aagaggtggc ttttttagca ggatactttg cgtcgaaggc ttctaaaacg ggtaagattg gatttgttgg aggagtgagg ggaaaagttt tagaatcttt tatgtatgga tatgaagctg gtgctaagta tgcaaactct aatattaaag tggtctctca atacgttggt acatttggag actttggact tggtcgttca acggcatcta atatgtatcg agatggggtt gatatcatat ttgcagctgc agggctttct ggtatagggg taattgaggc cgcaaaagag ttggggcccg atcattatat tattggagtc gatcaggatc aatcatatct tgctcctaac aatgttattg tttctgctgt aaaaaaagtt gattcattga tgtatagttt aacaaaaaag tatttagaaa ctggagtttt ggatggtggc aagaccatgt ttttagggct taaagaagat ggtcttggtt tagttttaaa tgaaaactta aaatcaaatt attctgagat ttataacaaa tcattgaaaa ttgggcaaag tataatgaat ggtataataa aagtgcctta tgacaaggta tcttatgata actttgtttt gcaaatggaa aattaa
Details for BobuA.19042.a.B2.GE39310
HARVESTED ON: data unavailable
SEQUENCED ON: 7/6/2015
EXPECTED MW: 36kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 91
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHMSD DGKSEAKTVS LIVDGAFDDK GFNESSSKAI RKLKADLNIN IIEKASTGNS YLGDIANLED GNSNLIWGIG FRLSDILFQR ASENVSVNYA IIEGVYDEIQ IPKNLLNISF RSEEVAFLAG YFASKASKTG KIGFVGGVRG KVLESFMYGY EAGAKYANSN IKVVSQYVGT FGDFGLGRST ASNMYRDGVD IIFAAAGLSG KGVIEAEKSW GPIIILLESI RINHILLLTM LLFLL
Validated NT Sequence
cacttaattt tccatttgca aaacaaagtt atcataagat accttgtcat aaggcacttt tattatacca ttcattatac tttgcccaat tttcaatgat ttgttataaa tctcagaata atttgatttt aagttttcat ttaaaactaa accaagacca tcttctttaa gccctaaaaa catggtcttg ccaccatcca aaactccagt ttctaaatac ttttttgtta aactatacat caatgaatca acttttttta cagcagaaac aataacattg ttaggagcaa gatatgattg atcctgatcg actccaataa tataatgatc gggccccaac tcttttcggc ctcaattacc cctttaccag aaagccctgc agctgcaaat atgatatcaa ccccatctcg atacatatta gatgccgttg aacgaccaag tccaaagtct ccaaatgtac caacgtattg agagaccact ttaatattag agtttgcata cttagcacca gcttcatatc catacataaa agattctaaa acttttcccc tcactcctcc aacaaatcca atcttacccg ttttagaagc cttcgacgca aagtatcctg ctaaaaaagc cacctcttcg gatctaaaac taatattaag aagatttttg ggtatttgaa tttcatcata aaccccttct atgattgcat aattaacaga aacattctcg ctagctcttt gaaaaagaat gtctgacaat ctaaacccaa ttccccaaat caaatttgaa ttaccatctt ctaagtttgc aatatctcct aaataagaat tgcctgtaga tgctttttca attatattta tatttaaatc tgcctttaat tttcttatcg ccttagaaga actttcatta aatcctttat catcaaaagc accatcaact ataagcgaaa ctgtttttgc ctccgacttg ccatcatcag acatatggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHSD DGKSEAKTVS LIVDGAFDDK GFNESSSKAI RKLKADLNIN IIEKASTGNS YLGDIANLED GNSNLIWGIG FRLSDILFQR ASENVSVNYA IIEGVYDEIQ IPKNLLNISF RSEEVAFLAG YFASKASKTG KIGFVGGVRG KVLESFMYGY EAGAKYANSN IKVVSQYVGT FGDFGLGRST ASNMYRDGVD IIFAAAGLSG IGVIEAAKEL GPDHYIIGVD QDQSYLAPNN VIVSAVKKVD SLMYSLTKKY LETGVLDGGK TMFLGLKEDG LGLVLNENLK SNYSEIYNKS LKIGQSIMNG IIKVPYDKVS YDNFVLQMEN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctgatg atggcaagtc ggaggcaaaa acagtttcgc ttatagttga tggtgctttt gatgataaag gatttaatga aagttcttct aaggcgataa gaaaattaaa ggcagattta aatataaata taattgaaaa agcatctaca ggcaattctt atttaggaga tattgcaaac ttagaagatg gtaattcaaa tttgatttgg ggaattgggt ttagattgtc agacattctt tttcaaagag ctagcgagaa tgtttctgtt aattatgcaa tcatagaagg ggtttatgat gaaattcaaa tacccaaaaa tcttcttaat attagtttta gatccgaaga ggtggctttt ttagcaggat actttgcgtc gaaggcttct aaaacgggta agattggatt tgttggagga gtgaggggaa aagttttaga atcttttatg tatggatatg aagctggtgc taagtatgca aactctaata ttaaagtggt ctctcaatac gttggtacat ttggagactt tggacttggt cgttcaacgg catctaatat gtatcgagat ggggttgata tcatatttgc agctgcaggg ctttctggta taggggtaat tgaggccgca aaagagttgg ggcccgatca ttatattatt ggagtcgatc aggatcaatc atatcttgct cctaacaatg ttattgtttc tgctgtaaaa aaagttgatt cattgatgta tagtttaaca aaaaagtatt tagaaactgg agttttggat ggtggcaaga ccatgttttt agggcttaaa gaagatggtc ttggtttagt tttaaatgaa aacttaaaat caaattattc tgagatttat aacaaatcat tgaaaattgg gcaaagtata atgaatggta taataaaagt gccttatgac aaggtatctt atgataactt tgttttgcaa atggaaaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat