BobuA.19038.a

M23 peptidase domain protein (BB_0262)

CENTER ID: BobuA.19038.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.19038.a.B3.GE39307 soluble domain #2 126 417
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.661
RefSeq: NP_212396.1
UniProt: O51277

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIIPKKKQRV EKRKKNFLFN SKKSVNFELK DFANISNIGK RRKKVFKIKN FFKKKINFFK KVSLFFYKLK IQNINHYEYK YYYKSLRDKV FDIFSVRFDY KLVFKLNAII FIFILTFYIN IFSYYGSYVF LNRLSLPKDY FIDTFLYYSD QDIAQISSYL PESNVSANVP GFKKNFVLKV FDHKIKPGET LSHVAARYQI TSETLISFNE IKDVRNIKPN SVIKVPNMKG IVYIVKKNDS ISSIASAYNV PKVDILDSNN LDNEVLFLGQ KLFIPGGRLP KDFLKEVLGE TFIYPVQGVI TSGYGYRPDP FTGVISFHNG IDIANLANTP IKASREGVVV TAGFNAGGYG KYIVISHSNG FQTLYAHLNS FAVKVGKKVS RGAVIGYMGS TGYSTGNHLH FTIFKNGKTE NPMKYLR
NT Sequence
atgattatac caaaaaaaaa gcaaagggta gaaaaacgta aaaaaaattt tttattcaat agcaaaaaga gtgttaattt tgaattaaaa gattttgcaa atatttctaa tattggtaaa agaagaaaaa aagtttttaa gattaagaac ttctttaaaa aaaaaatcaa tttttttaaa aaagtttctt tatttttcta taagcttaaa atacaaaata ttaatcatta tgaatataaa tattattaca agagcttgag agataaagtt tttgatatct ttagtgtaag attcgattat aaacttgttt ttaagcttaa tgcaataatc tttattttta tattaacatt ttatattaac attttttctt attatggctc gtatgttttt ttaaataggc tttctttgcc caaagattat tttattgata catttttata ttatagtgat caagatatag ctcaaattag tagttattta cctgaatcca atgtttctgc aaatgttcct ggatttaaaa aaaattttgt attaaaggta tttgatcata aaattaagcc tggagaaacg ctttcgcatg ttgcagctag atatcaaata accagtgaaa ctttaatttc ttttaatgaa attaaagatg taagaaatat taagccaaat tcagttatta aagttcccaa tatgaaagga attgtttata ttgttaaaaa aaatgactct atctcatcta tagctagtgc ttataatgtt cccaaggttg atattttaga ttctaataat cttgataatg aagttttatt tttagggcaa aagttgttta ttcctggggg aagattgccc aaagattttt taaaagaggt attaggggag acttttattt atcctgtgca gggtgttatt acttcggggt atggctatcg accagatccg tttacaggag ttattagttt tcacaatgga atagatattg caaatttagc taatacgcct attaaagcct caagagaagg tgttgttgta actgcaggat ttaatgcggg agggtatgga aaatatattg tcatttctca cagcaacgga ttccaaactt tatatgcaca tttgaattct tttgccgtta aggttggaaa aaaagtttca aggggagcgg taataggtta tatgggaagc actggctata gtacgggcaa tcatttgcat tttaccattt ttaaaaatgg taaaactgaa aatcctatga aatatttaag ataa
Details for BobuA.19038.a.B3.GE39307
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG SYVFLNRLSL PKDYFIDTFL YYSDQDIAQI SSYLPESNVS ANVPGFKKNF VLKVFDHKIK PGETLSHVAA RYQITSETLI SFNEIKDVRN IKPNSVIKVP NMKGIVYIVK KNDSISSIAS AYNVPKVDIL DSNNLDNEVL FLGQKLFIPG GRLPKDFLKE VLGETFIYPV QGVITSGYGY RPDPFTGVIS FHNGIDIANL ANTPIKASRE GVVVTAGFNA GGYGKYIVIS HSNGFQTLYA HLNSFAVKVG KKVSRGAVIG YMGSTGYSTG NHLHFTIFKN GKTENPMKYL R
Validated NT Sequence
atcttactca cttatcttaa atatttcata ggattttcag ttttaccatt tttaaaaatg gtaaaatgca aatgattgcc cgtactatag ccagtgcttc ccatataacc tattaccgct ccccttgaaa ctttttttcc aaccttaacg gcaaaagaat tcaaatgtgc atataaagtt tggaatccgt tgctgtgaga aatgacaata tattttccat accctcccgc attaaatcct gcagttacaa caacaccttc tcttgaggct ttaataggcg tattagctaa atttgcaata tctattccat tgtgaaaact aataactcct gtaaacggat ctggtcgata gccatacccc gaagtaataa caccctgcac aggataaata aaagtctccc ctaatacctc ttttaaaaaa tctttgggca atcttccccc aggaataaac aacttttgcc ctaaaaataa aacttcatta tcaagattat tagaatctaa aatatcaacc ttgggaacat tataagcact agctatagat gagatagagt catttttttt aacaatataa acaattcctt tcatattggg aactttaata actgaatttg gcttaatatt tcttacatct ttaatttcat taaaagaaat taaagtttca ctggttattt gatatctagc tgcaacatgc gaaagcgttt ctccaggctt aattttatga tcaaatacct ttaatacaaa atttttttta aatccaggaa catttgcaga aacattggat tcaggtaaat aactactaat ttgagctata tcttgatcac tataatataa aaatgtatca ataaaataat ctttgggcaa agaaagccta tttaaaaaaa catacgagcc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHGS YVFLNRLSLP KDYFIDTFLY YSDQDIAQIS SYLPESNVSA NVPGFKKNFV LKVFDHKIKP GETLSHVAAR YQITSETLIS FNEIKDVRNI KPNSVIKVPN MKGIVYIVKK NDSISSIASA YNVPKVDILD SNNLDNEVLF LGQKLFIPGG RLPKDFLKEV LGETFIYPVQ GVITSGYGYR PDPFTGVISF HNGIDIANLA NTPIKASREG VVVTAGFNAG GYGKYIVISH SNGFQTLYAH LNSFAVKVGK KVSRGAVIGY MGSTGYSTGN HLHFTIFKNG KTENPMKYLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctcgt atgttttttt aaataggctt tctttgccca aagattattt tattgataca tttttatatt atagtgatca agatatagct caaattagta gttatttacc tgaatccaat gtttctgcaa atgttcctgg atttaaaaaa aattttgtat taaaggtatt tgatcataaa attaagcctg gagaaacgct ttcgcatgtt gcagctagat atcaaataac cagtgaaact ttaatttctt ttaatgaaat taaagatgta agaaatatta agccaaattc agttattaaa gttcccaata tgaaaggaat tgtttatatt gttaaaaaaa atgactctat ctcatctata gctagtgctt ataatgttcc caaggttgat attttagatt ctaataatct tgataatgaa gttttatttt tagggcaaaa gttgtttatt cctgggggaa gattgcccaa agatttttta aaagaggtat taggggagac ttttatttat cctgtgcagg gtgttattac ttcggggtat ggctatcgac cagatccgtt tacaggagtt attagttttc acaatggaat agatattgca aatttagcta atacgcctat taaagcctca agagaaggtg ttgttgtaac tgcaggattt aatgcgggag ggtatggaaa atatattgtc atttctcaca gcaacggatt ccaaacttta tatgcacatt tgaattcttt tgccgttaag gttggaaaaa aagtttcaag gggagcggta ataggttata tgggaagcac tggctatagt acgggcaatc atttgcattt taccattttt aaaaatggta aaactgaaaa tcctatgaaa tatttaagat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat