SSGCID: Target Page

BobuA.18992.a

Uncharacterized protein BB_0173

CENTER ID:	BobuA.18992.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18992.a.B2.GE39082	trimmed VWFA domain		82	341
BobuA.18992.a.B3.GE39083	soluble domain		85	317

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.570
UniProt:	O51195

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

LKNLIEVLML TFNEPLYLFL LVIFPLIIYF NHFLKNRGGK IKFPISLYGN FNSLKLKDYR LNLMYFFTYS FLYLAAMVMV FALAGPSVSK KKMIHLSAGA DIVIVLDISP SMGAVEFSSK NRLEFSKELI RGFISQREND NIGLVAFAKD ASIVVPITTD REFFNKKLDD IYIMDLGNGS ALGLGISIAL SHLKHSEALK RSIVVLTDGV VNSDEIXKDQ VINLAQGLNV KIYSIGIGSS EEFSVEFKLR SGKFYQGSFK EVYDPSMLVE ISNKTGGLFY SVNDDFSFQF AIQDFSKKEN LERKIKIAVD NKDIYKEFLV LAFCLLLVYF IFSKIFLKEI L

NT Sequence

ttgaagaatt taatagaggt gctaatgtta acatttaatg agcctttata tttgttttta ttagtaattt ttcctttaat aatttacttt aatcattttt taaaaaatag aggaggcaag ataaagtttc caatatcgct ttacggcaat ttcaactctt taaaacttaa ggattataga ttaaatttga tgtatttttt tacttactct tttttatatt tagctgcaat ggttatggtg tttgctttag caggtccttc agtttcaaaa aagaagatga tacatcttag tgctggtgct gacattgtca ttgttcttga catatcgcct agtatggggg ctgttgagtt ttcttccaaa aatagacttg aattttcaaa agaattgatt agaggtttta tttctcaacg tgaaaatgat aatattggtt tggtagcttt tgcaaaagat gcttcaatag tagtgcctat aacaacagat agggaatttt ttaataaaaa gctagatgat atttatatta tggatcttgg caatggttct gctttagggc taggtatttc tattgcgctg tctcatttaa agcattctga ggctcttaaa agatcaatag tggttttaac agatggggtt gttaattcag atgagattya taaagatcaa gtgatcaatc ttgctcaagg tttaaatgtt aagatttatt ctattggtat tggaagttct gaagaattta gtgttgagtt taaattaaga tctggaaaat tttatcaggg aagttttaaa gaggtttatg atcctagtat gcttgtggag atttcaaata aaacgggggg gcttttttat tcggttaatg atgatttttc ttttcaattt gcaattcaag atttttcaaa aaaggaaaat ttggagagaa aaattaaaat agctgtggac aataaagata tttataaaga atttttagtt ttagcgtttt gcttattgct tgtttatttt attttttcaa aaattttctt aaaagagata ctatga

Details for BobuA.18992.a.B2.GE39082

HARVESTED ON:	3/31/2015
SEQUENCED ON:	3/27/2015
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA LAGPSVSKKK MIHLSAGADI VIVLDISPSM GAVEFSSKNR LEFSKELIRG FISQRENDNI GLVAFAKDAS IVVPITKDRE FFNKKLDDIY IMDLGNGSAL GLGISIALSH LKHSEALKRS IVVLTDGVVN SDEIYKDQVI NLAQGLNVKI YSIGIGSSEE FSVEFKLRSG KFYQRSFKEV YDPSMLVEIS NKTGGLFYSV NDDFSFQFAI QDFSKKENLE RKIKIAVDNK DIYKEFLVLA FCLLLVYFIF SKIFLKEIL

Validated NT Sequence

atcttactca cttatagtat ctcttttaag aaaatttttg aaaaaataaa ataaacaagc aataagcaaa acgctaaaac taaaaattct ttataaatat ctttattgtc cacagctatt ttaatttttc tctccaaatt ttcctttttt gaaaaatctt gaattgcaaa ttgaaaagaa aaatcatcat taaccgaata aaaaagcccc cccgttttat ttgaaatctc cacaagcata ctaggatcat aaacctcttt aaaacttctc tgataaaatt ttccagatct taatttaaac tcaacactaa attcttcaga acttccaata ccaatagaat aaatcttaac atttaaacct tgagcaagat tgatcacttg atctttataa atctcatctg aattaacaac cccatctgtt aaaaccacta ttgatctttt aagagcctca gaatgcttta aatgagacag cgcaatagaa atacctagcc ctaaagcaga accattgcca agatccataa tataaatatc atctagcttt ttattaaaaa attccctatc ttttgttata ggcactacta ttgaagcatc ttttgcaaaa gctaccaaac caatattatc attttcacgt tgagaaataa aacctctaat caattctttt gaaaattcaa gtctattttt ggaagaaaac tcaacagccc ccatactagg cgatatgtca agaacaatga caatgtcagc accagcacta agatgtatca tcttcttttt tgaaactgaa ggacctgcta aagccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHAL AGPSVSKKKM IHLSAGADIV IVLDISPSMG AVEFSSKNRL EFSKELIRGF ISQRENDNIG LVAFAKDASI VVPITTDREF FNKKLDDIYI MDLGNGSALG LGISIALSHL KHSEALKRSI VVLTDGVVNS DEIXKDQVIN LAQGLNVKIY SIGIGSSEEF SVEFKLRSGK FYQGSFKEVY DPSMLVEISN KTGGLFYSVN DDFSFQFAIQ DFSKKENLER KIKIAVDNKD IYKEFLVLAF CLLLVYFIFS KIFLKEIL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctttag caggtccttc agtttcaaaa aagaagatga tacatcttag tgctggtgct gacattgtca ttgttcttga catatcgcct agtatggggg ctgttgagtt ttcttccaaa aatagacttg aattttcaaa agaattgatt agaggtttta tttctcaacg tgaaaatgat aatattggtt tggtagcttt tgcaaaagat gcttcaatag tagtgcctat aacaacagat agggaatttt ttaataaaaa gctagatgat atttatatta tggatcttgg caatggttct gctttagggc taggtatttc tattgcgctg tctcatttaa agcattctga ggctcttaaa agatcaatag tggttttaac agatggggtt gttaattcag atgagattya taaagatcaa gtgatcaatc ttgctcaagg tttaaatgtt aagatttatt ctattggtat tggaagttct gaagaattta gtgttgagtt taaattaaga tctggaaaat tttatcaggg aagttttaaa gaggtttatg atcctagtat gcttgtggag atttcaaata aaacgggggg gcttttttat tcggttaatg atgatttttc ttttcaattt gcaattcaag atttttcaaa aaaggaaaat ttggagagaa aaattaaaat agctgtggac aataaagata tttataaaga atttttagtt ttagcgtttt gcttattgct tgtttatttt attttttcaa aaattttctt aaaagagata ctatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BobuA.18992.a.B3.GE39083

HARVESTED ON:	3/31/2015
SEQUENCED ON:	3/27/2015
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG PSVSKKKMIH LSAGADIVIV LDISPSMGAV EFSSKNRLEF SKELIRGFIS QRENDNIGLV AFAKDASIVV PITTDREFFN KKLDDIYIMD LGNGSALGLG ISIALSHLKH SEALKRSIVV LTDGVVNSDE IYKDQVINLA QGLNVKIYSI GIGSSEEFSV EFKLRSGKFY QGSFKEVYDP SMLVEISNKT GGLFYSVNDD FSFQFAIQDF SKKENLERKI KIAVDNKDIY KE

Validated NT Sequence

atcttactca cttattcttt ataaatatct ttattgtcca cagctatttt aatttttctc tccaaatttt ccttttttga aaaatcttga attgcaaatt gaaaagaaaa atcatcatta accgaataaa aaagcccccc cgttttattt gaaatctcca caagcatact aggatcataa acctctttaa aacttccctg ataaaatttt ccagatctta atttaaactc aacactaaat tcttcagaac ttccaatacc aatagaataa atcttaacat ttaaaccttg agcaagattg atcacttgat ctttataaat ctcatctgaa ttaacaaccc catctgttaa aaccactatt gatcttttaa gagcctcaga atgctttaaa tgagacagcg caatagaaat acctagccct aaagcagaac cattgccaag atccataata taaatatcat ctagcttttt attaaaaaat tccctatctg ttgttatagg cactactatt gaagcatctt ttgcaaaagc taccaaacca atattatcat tttcacgttg agaaataaaa cctctaatca attcttttga aaattcaagt ctatttttgg aagaaaactc aacagccccc atactaggcg atatgtcaag aacaatgaca atgtcagcac cagcactaag atgtatcatc ttcttttttg aaactgaagg acccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHGP SVSKKKMIHL SAGADIVIVL DISPSMGAVE FSSKNRLEFS KELIRGFISQ RENDNIGLVA FAKDASIVVP ITTDREFFNK KLDDIYIMDL GNGSALGLGI SIALSHLKHS EALKRSIVVL TDGVVNSDEI XKDQVINLAQ GLNVKIYSIG IGSSEEFSVE FKLRSGKFYQ GSFKEVYDPS MLVEISNKTG GLFYSVNDDF SFQFAIQDFS KKENLERKIK IAVDNKDIYK E

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtcctt cagtttcaaa aaagaagatg atacatctta gtgctggtgc tgacattgtc attgttcttg acatatcgcc tagtatgggg gctgttgagt tttcttccaa aaatagactt gaattttcaa aagaattgat tagaggtttt atttctcaac gtgaaaatga taatattggt ttggtagctt ttgcaaaaga tgcttcaata gtagtgccta taacaacaga tagggaattt tttaataaaa agctagatga tatttatatt atggatcttg gcaatggttc tgctttaggg ctaggtattt ctattgcgct gtctcattta aagcattctg aggctcttaa aagatcaata gtggttttaa cagatggggt tgttaattca gatgagatty ataaagatca agtgatcaat cttgctcaag gtttaaatgt taagatttat tctattggta ttggaagttc tgaagaattt agtgttgagt ttaaattaag atctggaaaa ttttatcagg gaagttttaa agaggtttat gatcctagta tgcttgtgga gatttcaaat aaaacggggg ggctttttta ttcggttaat gatgattttt cttttcaatt tgcaattcaa gatttttcaa aaaaggaaaa tttggagaga aaaattaaaa tagctgtgga caataaagat atttataaag aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat