BobuA.18991.a

Uncharacterized protein (BB_B26)

CENTER ID: BobuA.18991.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18991.a.B1.GE39010 full length 1 231
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1613
RefSeq: NP_047012.1
UniProt: O50997

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNENKFSSCI ENSKAYSDYI TSKHKILLIP VPIVKIAMGE DLKIFEISFQ DKNKDFPGYI QLNKKSLYIN ENMSLENKRF TIAKYLGHYL MHQDQIKNLS KNENYYNNIQ DSQMVTEANI FAANILIPTT TLKLKLSQYK SKEYPQKTIA KEFQVTENII YLKLSIINDL VKIDKIKKSK KFLKIKNTKE KIEANIYLHN TDKIKESIAL DLEKYELEKK ERIKKIFEDL E
NT Sequence
atgaatgaaa acaaattcag ctcttgtatt gaaaattcta aagcctattc ggattatata acatcaaaac ataaaatatt acttattcct gttcctatag taaaaattgc catgggagaa gatcttaaaa ttttcgaaat aagttttcaa gataagaata aagattttcc aggatatatt caattaaata aaaaatcttt atatataaat gaaaacatga gccttgaaaa taaaagattt acaatagcaa aatacttggg gcattatttg atgcatcaag atcaaataaa aaatctatct aaaaatgaaa actattataa caacattcaa gatagtcaaa tggtaacaga agctaacata tttgcagcaa acatattgat cccaacaacc acattaaaat taaaattatc tcaatataaa tctaaagagt atccccaaaa aacaatagca aaagaattcc aagtaactga aaatataatt tatttaaaat taagcataat taatgatctt gtcaaaatag ataaaataaa aaagagtaaa aaatttttaa aaattaaaaa cacaaaagaa aaaatagaag ccaacatcta cctccacaat acagataaaa ttaaagaatc aatagctctt gatttggaaa aatatgaact tgaaaaaaaa gaacgaatta aaaaaatatt tgaagatcta gagtaa
Details for BobuA.18991.a.B1.GE39010
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN ENKFSSCIEN SKAYSDYITS KHKILLIPVP IVKIAMGEDL KIFEISFQDK NKDFPGYIQL NKKSLYINEN MSLENKRFTI AKYLGHYLMH QDQIKNLSKN ENYYNNIQDS QMVTEANIFA ANILIPTTTL KLKLTQYKSK EYPQKTIAKE FQVTENIIYL KLSIINDLVK IDKIKKSKKF LKIKNTKEKI EANIYLHNTD KIKESIALDL EKYELEKKER IKKIFEDLE
Validated NT Sequence
atcttactca cttactctag atcttcaaat atttttttaa ttcgttcttt tttttcaagt tcatattttt ccaaatcaag agctattgat tctttaattt tatctgtatt gtggaggtag atgttggctt ctattttttc ttttgtgttt ttaattttta aaaatttttt actctttttt attttatcta ttttgacaag atcattaatt atgcttaatt ttaaataaat tatattttca gttacttgga attcttttgc tattgttttt tggggatact ctttagattt atattgagtt aattttaatt ttaatgtggt tgttgggatc aatatgtttg ctgcaaatat gttagcttct gttaccattt gactatcttg aatgttgtta taatagtttt catttttaga tagatttttt atttgatctt gatgcatcaa ataatgcccc aagtattttg ctattgtaaa tcttttattt tcaaggctca tgttttcatt tatatataaa gattttttat ttaattgaat atatcctgga aaatctttat tcttatcttg aaaacttatt tcgaaaattt taagatcttc tcccatggca atttttacta taggaacagg aataagtaat attttatgtt ttgatgttat ataatccgaa taggctttag aattttcaat acaagagctg aatttgtttt cattcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMN ENKFSSCIEN SKAYSDYITS KHKILLIPVP IVKIAMGEDL KIFEISFQDK NKDFPGYIQL NKKSLYINEN MSLENKRFTI AKYLGHYLMH QDQIKNLSKN ENYYNNIQDS QMVTEANIFA ANILIPTTTL KLKLSQYKSK EYPQKTIAKE FQVTENIIYL KLSIINDLVK IDKIKKSKKF LKIKNTKEKI EANIYLHNTD KIKESIALDL EKYELEKKER IKKIFEDLE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg aaaacaaatt cagctcttgt attgaaaatt ctaaagccta ttcggattat ataacatcaa aacataaaat attacttatt cctgttccta tagtaaaaat tgccatggga gaagatctta aaattttcga aataagtttt caagataaga ataaagattt tccaggatat attcaattaa ataaaaaatc tttatatata aatgaaaaca tgagccttga aaataaaaga tttacaatag caaaatactt ggggcattat ttgatgcatc aagatcaaat aaaaaatcta tctaaaaatg aaaactatta taacaacatt caagatagtc aaatggtaac agaagctaac atatttgcag caaacatatt gatcccaaca accacattaa aattaaaatt atctcaatat aaatctaaag agtatcccca aaaaacaata gcaaaagaat tccaagtaac tgaaaatata atttatttaa aattaagcat aattaatgat cttgtcaaaa tagataaaat aaaaaagagt aaaaaatttt taaaaattaa aaacacaaaa gaaaaaatag aagccaacat ctacctccac aatacagata aaattaaaga atcaatagct cttgatttgg aaaaatatga acttgaaaaa aaagaacgaa ttaaaaaaat atttgaagat ctagagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t