BobuA.18987.a

Telomere resolvase ResT (EC 3.1.22.-) (BB_B03)

CENTER ID: BobuA.18987.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18987.a.B3.GE40900 ResT pdb_2V6E 9 435
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1592
RefSeq: NP_046989.1
UniProt: O50979

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPKVKIKND FEIFRKELEI LYKKYLNNEL SYLKLKEKLK ILAENHKAIL FRKDKFTNRS IILNLSKTRK IIKEYINLSV IERIRRDNTF LFFWKSRRIK ELKNIGIKDR KKIEELIFSN QMNDEKSYFQ YFIDLFVTPK WLNDYAHKYK IEKINSYRKE QIFVKINLNT YIEIIKLLLN QSRDIRLKFY GVLMAIGRRP VEVMKLSQFY IADKNHIRME FIAKKRENNI VNEVVFPVFA DPELIINSIK EIRYMEQTEN LTKEIISSNL AYSYNRLFRQ IFNNIFAPEE SVYFCRAIYC KFSYLAFAPK NMEMNYWITK VLGHEPNDIT TAFHYNRYVL DNLDDKADNS LLTLLNQRIY TYVRRKATYS TLTMDRLESL IKEHHIFDDN YIKTLIVIKN LMLKDNLETL AMVRGLNVKI RKAFKATYGY NYNYIKLTEY LSIIFNYKL
NT Sequence
atgcctccaa aagtgaagat aaaaaatgat tttgaaatat ttagaaaaga attagaaatt ctatataaaa aatatctcaa taacgagctt tcatatttaa aattgaaaga aaaactaaaa attcttgcag agaatcacaa agctattctt tttagaaaag ataaatttac aaatcgttca ataattttaa atctttcaaa gactcgtaaa ataattaaag aatatattaa tctttcagta attgaaagga ttagaagaga taatactttt ttattttttt ggaaatcaag aagaataaaa gaattaaaaa atataggaat taaagatcga aaaaaaatag aagagttgat attttcaaat caaatgaatg atgagaagtc ctattttcaa tattttatag atttgtttgt aactccaaaa tggttaaatg attatgctca taaatataaa attgaaaaaa ttaatagtta taggaaagag cagatatttg ttaaaattaa tttaaatacc tatattgaaa taattaagct tctactgaat caaagtcgag atattagatt aaaattttat ggagttttaa tggcaatagg acgtcgtcct gttgaagtaa tgaagctttc acaattttat atcgcagata aaaatcatat tcgtatggag tttattgcaa aaaagcgaga aaataatatt gttaatgaag ttgtttttcc agtttttgca gatcctgaat taataattaa ttccataaaa gaaatacgct atatggagca gactgagaat cttactaaag agataatctc ttctaatctt gcatacagtt ataatagatt gtttcgtcaa atttttaata atatttttgc tcctgaagag tctgtctatt tttgcagagc aatttattgt aaattttctt atcttgcatt tgcgcctaaa aacatggaaa tgaattattg gataacaaaa gttttggggc acgagccaaa tgacataaca acggcctttc attataatcg gtatgtttta gataatttag acgataaggc agataatagc ttattaacat tgcttaatca aagaatttat acatatgtta gacgtaaagc tacttattct actcttacaa tggatcgttt agaaagtttg ataaaagaac atcatatatt tgatgataat tatattaaaa cgttgattgt aattaaaaat ttgatgttaa aggataattt agaaactttg gcaatggtta gaggattgaa tgttaaaatt cgcaaagctt ttaaagccac atatggatac aattataact atataaaact tacagaatac ttatcaataa tttttaatta taagctatag
Details for BobuA.18987.a.B3.GE40900
HARVESTED ON: 12/20/2016
SEQUENCED ON: 12/27/2016
EXPECTED MW: 53kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN DFEIFRKKLE ILYKKYLNNE LSYLKLKEKL KILAENHKAI LFRKDKFTNR SIILNLSKTR KIIKEYINLS VIERIRRDNT FLFFWKSRRI KELKNIGIKD RKKIEELIFS NQMNDEKSYF QYFIDLFVTP KWLNDYAHKY KIEKINSYRK EQIFVKINLN TYIEIIKLLL NQSRDIRLKF YGVLMAIGRR PVEVMKLSQF YIADKNHIRM EFIAKKRENN IVNEVVFPVF ADPELIINSI KEIRYMEQTE NLTKEIISSN LAYSYNRLFR QIFNNIFAPE ESVYFCRAIY CKFSYLAFAP KNMEMNYWIT KVLGHEPNDI TTAFHYNRYV LDNLDDKADN SLLTLLNQRI YTYVRRKATY STLTMDRLES LIKEHHIFDD NYIKTLIVIK NLMLKDNLET LAMVRGLNVK IRKAFKATY
Validated NT Sequence
catatgtggc tttaaaagct ttgcgaattt taacattcaa tcctctaacc attgccaaag tttctaaatt atcctttaac atcaaatttt taattacaat caacgtttta atataattat catcaaatat atgatgttct tttatcaaac tttctaaacg atccattgta agagtagaat aagtagcttt acgtctaaca tatgtataaa ttctttgatt aagcaatgtt aataagctat tatctgcctt atcgtctaaa ttatctaaaa cataccgatt ataatgaaag gccgttgtta tgtcatttgg ctcgtgcccc aaaacttttg ttatccaata attcatttcc atgtttttag gcgcaaatgc aagataagaa aatttacaat aaattgctct gcaaaaatag acagactctt caggagcaaa aatattatta aaaatttgac gaaacaatct attataactg tatgcaagat tagaagagat tatctcttta gtaagattct cagtctgctc catatagcgt atttctttta tggaattaat tattaattca ggatctgcaa aaactggaaa aacaacttca ttaacaatat tattttctcg cttttttgca ataaactcca tacgaatatg atttttatct gcgatataaa attgtgaaag cttcattact tcaacaggac gacgtcctat tgccattaaa actccataaa attttaatct aatatctcga ctttgattca gtagaagctt aattatttca atataggtat ttaaattaat tttaacaaat atctgctctt tcctataact attaattttt tcaattttat atttatgagc ataatcattt aaccattttg gagttacaaa caaatctata aaatattgaa aataggactt ctcatcattc atttgatttg aaaatatcaa ctcttctatt ttttttcgat ctttaattcc tatatttttt aattctttta ttcttcttga tttccaaaaa aataaaaaag tattatctct tctaatcctt tcaattactg aaagattaat atattcttta attattttac gagtctttga aagatttaaa attattgaac gatttgtaaa tttatctttt ctaaaaagaa tagctttgtg attctctgca agaattttta gtttttcttt caattttaaa tatgaaagct cgttattgag atatttttta tatagaattt ctaatttttt tctaaatatt tcaaaatcat tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHND FEIFRKELEI LYKKYLNNEL SYLKLKEKLK ILAENHKAIL FRKDKFTNRS IILNLSKTRK IIKEYINLSV IERIRRDNTF LFFWKSRRIK ELKNIGIKDR KKIEELIFSN QMNDEKSYFQ YFIDLFVTPK WLNDYAHKYK IEKINSYRKE QIFVKINLNT YIEIIKLLLN QSRDIRLKFY GVLMAIGRRP VEVMKLSQFY IADKNHIRME FIAKKRENNI VNEVVFPVFA DPELIINSIK EIRYMEQTEN LTKEIISSNL AYSYNRLFRQ IFNNIFAPEE SVYFCRAIYC KFSYLAFAPK NMEMNYWITK VLGHEPNDIT TAFHYNRYVL DNLDDKADNS LLTLLNQRIY TYVRRKATYS TLTMDRLESL IKEHHIFDDN YIKTLIVIKN LMLKDNLETL AMVRGLNVKI RKAFKATYGY NYNYI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatgatt ttgaaatatt tagaaaagaa ttagaaattc tatataaaaa atatctcaat aacgagcttt catatttaaa attgaaagaa aaactaaaaa ttcttgcaga gaatcacaaa gctattcttt ttagaaaaga taaatttaca aatcgttcaa taattttaaa tctttcaaag actcgtaaaa taattaaaga atatattaat ctttcagtaa ttgaaaggat tagaagagat aatacttttt tatttttttg gaaatcaaga agaataaaag aattaaaaaa tataggaatt aaagatcgaa aaaaaataga agagttgata ttttcaaatc aaatgaatga tgagaagtcc tattttcaat attttataga tttgtttgta actccaaaat ggttaaatga ttatgctcat aaatataaaa ttgaaaaaat taatagttat aggaaagagc agatatttgt taaaattaat ttaaatacct atattgaaat aattaagctt ctactgaatc aaagtcgaga tattagatta aaattttatg gagttttaat ggcaatagga cgtcgtcctg ttgaagtaat gaagctttca caattttata tcgcagataa aaatcatatt cgtatggagt ttattgcaaa aaagcgagaa aataatattg ttaatgaagt tgtttttcca gtttttgcag atcctgaatt aataattaat tccataaaag aaatacgcta tatggagcag actgagaatc ttactaaaga gataatctct tctaatcttg catacagtta taatagattg tttcgtcaaa tttttaataa tatttttgct cctgaagagt ctgtctattt ttgcagagca atttattgta aattttctta tcttgcattt gcgcctaaaa acatggaaat gaattattgg ataacaaaag ttttggggca cgagccaaat gacataacaa cggcctttca ttataatcgg tatgttttag ataatttaga cgataaggca gataatagct tattaacatt gcttaatcaa agaatttata catatgttag acgtaaagct acttattcta ctcttacaat ggatcgttta gaaagtttga taaaagaaca tcatatattt gatgataatt atattaaaac gttgattgta attaaaaatt tgatgttaaa ggataattta gaaactttgg caatggttag aggattgaat gttaaaattc gcaaagcttt taaagccaca tatggataca attataacta tatatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat