BobuA.18983.a

Uncharacterized protein (BB_A30)

CENTER ID: BobuA.18983.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18983.a.B1.GE39004 full length 1 197
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1545
RefSeq: NP_045703.1
UniProt: O50923

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIFKNRKEEY IFLFKKGLKD LDIAQILGVS ESFVAKARNK YFKSGDSVCK KNSFSSDLAE ESTTKVSFNE DNFNNLVLLA TKKACELENA KNEAERLFYE IVCSFIDSHH RCKNLSLAAI KKEVLILDLK ISKLQSEISA NKKRGLKSQE SLERELADRI KKREEIEKKL ITICMREDFD TLVKLKSVLS SKNLKLE
NT Sequence
atgatattta aaaatagaaa agaagaatat atttttttat ttaaaaaagg tttaaaagat ttagatatcg ctcaaatttt aggtgttagt gaatcttttg tagcaaaagc ccgaaataaa tattttaaat ccggtgattc tgtttgtaaa aagaattctt tttctagcga cctggcagaa gaaagcacta ctaaagtgtc ttttaatgag gacaatttta acaatttagt gctgcttgct acaaaaaagg cctgtgagct tgaaaatgcc aaaaatgagg ctgaaaggtt attttacgag atagtttgtt cttttattga ttcccaccac agatgtaaaa atctcagttt agcggcaata aaaaaggaag tattaatttt ggatttaaaa attagcaaac ttcaaagcga gattagcgcc aataaaaaaa gagggcttaa aagccaagaa agccttgaaa gagagcttgc tgataggatt aaaaagagag aagagattga aaaaaaactt attactattt gcatgagaga ggattttgat actcttgtta agcttaaatc tgttttaagt agtaagaatt taaaattgga gtaa
Details for BobuA.18983.a.B1.GE39004
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 24kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI FKNRKEEYIF LFKKGLKDLD IAQILGVSES FVTKARNKYF KSGDSVCKKN SFSSDLAEES TTKVSFNEDN FNNLVLLATK KACELENAKN EAERLFYEIV CSFIDSHHRC KNLSLAAIKK EVLILDLKIS KLQSEIGANK KRGLKSQESL ERELADRIKK REEIEKKLIT ICMREDFDTL VKLKSVLSSK NLKLE
Validated NT Sequence
atcttactca cttactccaa ttttaaattc ttactactta aaacagattt aagcttaaca agagtatcaa aatcctctct catgcaaata gtaataagtt ttttttcaat ctcttctctc tttttaatcc tatcagcaag ctctctttca aggctttctt ggcttttaag ccctcttttt ttattggcgc caatctcgct ttgaagtttg ctaattttta aatccaaaat taatacttcc ttttttattg ccgctaaact gagattttta catctgtggt gggagtcaat aaaagaacaa actatctcgt aaaataacct ttcagcctca tttttggcat tttcaagctc acaggccttt tttgtagcaa gcagcactaa attgttaaaa ttgtcctcat taaaagacac tttagtagtg ctttcttctg ccaggtcgct agaaaaagaa ttctttttac aaacagaatc accggattta aaatatttat ttcgggcttt tgttacaaaa gattcactaa cacctaaaat ttgagcgata tctaaatctt ttaaaccttt tttaaataaa aaaatatatt cttcttttct atttttaaat atcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMI FKNRKEEYIF LFKKGLKDLD IAQILGVSES FVAKARNKYF KSGDSVCKKN SFSSDLAEES TTKVSFNEDN FNNLVLLATK KACELENAKN EAERLFYEIV CSFIDSHHRC KNLSLAAIKK EVLILDLKIS KLQSEISANK KRGLKSQESL ERELADRIKK REEIEKKLIT ICMREDFDTL VKLKSVLSSK NLKLE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatat ttaaaaatag aaaagaagaa tatatttttt tatttaaaaa aggtttaaaa gatttagata tcgctcaaat tttaggtgtt agtgaatctt ttgtagcaaa agcccgaaat aaatatttta aatccggtga ttctgtttgt aaaaagaatt ctttttctag cgacctggca gaagaaagca ctactaaagt gtcttttaat gaggacaatt ttaacaattt agtgctgctt gctacaaaaa aggcctgtga gcttgaaaat gccaaaaatg aggctgaaag gttattttac gagatagttt gttcttttat tgattcccac cacagatgta aaaatctcag tttagcggca ataaaaaagg aagtattaat tttggattta aaaattagca aacttcaaag cgagattagc gccaataaaa aaagagggct taaaagccaa gaaagccttg aaagagagct tgctgatagg attaaaaaga gagaagagat tgaaaaaaaa cttattacta tttgcatgag agaggatttt gatactcttg ttaagcttaa atctgtttta agtagtaaga atttaaaatt ggagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat