BobuA.18975.a

Methyl-accepting chemotaxis protein (BB_0597)

CENTER ID: BobuA.18975.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18975.a.B3.GE39002 soluble domain N-term 39 323
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.988
RefSeq: NP_212731.1
UniProt: O51543

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDENLIDVN LKNTKRFLYL VLFGFLFLNF LFIGYAYMNH KNEYLDRFKF DSKLFLNSVS AVIKAKYSES SRFLEELIRD SYRFGILVNS SKSFLLSSSL KLGDSLDENS DLFLRSREFN SIDKIFKTIP LAEDSLEGIF YIPIGKNVLI SNSNFSSLGL KDVRLDPIYS VPVEKNSKYY SRYMQIDGKI YSIISFPVRD SVSTLGVIGI LICFDESLDI IENQLYSSLK FGSKNYNFFM LDRNYMPIFS NLNNLQAKSF STAYSENFLS KVIAYAKKDS SSSQYTFNYE RDFYSLNFVK TDDFLTQGLI LNVNSIPIMF KSNWVIFVAF LLLSFAIIFY LCNTFVFSLI NDFNRIVDYQ KSKSDPFSLE SPLEVKYSSS IISYISSKLD NLSSKSNESF EKIKFYSEDL NEYLEQIETA ISNTESIDSS ILVYEQLRDT FSRFEKSIVD ILKGFESIAD PINDHNKYIS EISSNFEESV SFFYSIDKNL EIFNKVATIN STDIENIKSK VFDLNIVFEN VNKNFADLLS QTNSLQSVNK LLVSISAQTN MLAMNAAIEA AKAGDAGKSF AVVAEEIRKL AINSGKYSKT IKDELKTVDS IIAVINSEID TIYKNFIDIQ DNVDNNFSRH EKVDLTLAKH FKEIGEFKER YLSHDTKIRD AKNMYKEIFN NHYFISGKFN NFSQDLKEFK VSKMNLDAVS SLQEYSSLVK SSKDKILKTK ELIQKINDEI KDILF
NT Sequence
atgacagatg agaatttaat tgatgttaat ctgaaaaata ctaaacgatt tctttatcta gttttatttg gattcctttt tttaaatttt ttattcatag gctatgctta tatgaatcat aaaaatgaat atttggatcg ctttaaattt gattctaagt tgtttttaaa cagtgtgtct gctgttatta aagccaaata ttcggaatcc tctaggtttc ttgaggagct tataagagat agctataggt ttgggatatt ggtgaattct tcaaaaagct ttcttttgtc ttcaagttta aaattaggtg atagtttgga tgaaaatagt gatttgtttt taaggtcaag ggaatttaac tctatagata aaatttttaa aactattcct ttagcagaag attcacttga aggtatattt tatattccta tagggaaaaa tgttttaata tcaaattcaa atttttcatc tttaggctta aaagatgtta gattggatcc aatttattct gttcctgtag aaaaaaattc taagtattat tcaagatata tgcaaataga tgggaaaatt tattctataa taagttttcc agttagagat tctgtttcaa cattgggtgt gatagggatt ttaatatgct ttgatgagtc gttagatatt attgaaaatc agttgtattc ttctcttaaa tttggtagta aaaattataa tttttttatg cttgacagaa attacatgcc cattttttca aaccttaata atcttcaggc caaatctttt tctacagctt atagtgagaa ttttttgagt aaagttatag cttatgctaa aaaagattct tctagctctc agtacacttt taattatgaa agagattttt attctttaaa ctttgtaaaa accgatgatt ttttgactca ggggcttatt ttaaatgtca attccattcc tattatgttt aaatcaaatt gggttatatt tgttgcattt ttattattgt cttttgcaat tattttttat ttatgcaata cttttgtttt ttcattaatt aatgatttta acagaattgt tgactatcaa aaatcaaaaa gcgatccttt tagtcttgaa tctcccttag aggttaagta ttcttcatct attatttctt atattagttc aaagctagat aatctgtctt ctaagagtaa tgaatctttt gagaagataa aattttattc tgaagatttg aatgaatatt tggaacaaat agaaactgct atatcaaata ctgagagtat agattctagc attttagttt acgaacaact aagagatact ttttctagat ttgaaaaatc aattgttgat attttaaaag gctttgaatc tattgctgat ccgattaatg atcacaataa atatatatca gaaatctctt caaattttga agagagtgtt agttttttct atagtataga taaaaattta gaaattttta ataaggttgc tactataaat tctactgata ttgaaaatat taaaagtaag gtttttgatt taaatattgt ttttgaaaat gtgaataaaa attttgcaga tcttttgtct caaacaaata gtttgcaaag tgtaaataaa cttttagttt caatttcagc tcagaccaat atgcttgcta tgaatgcagc aattgaagca gcaaaagcag gtgatgcagg taaaagtttt gcagttgttg ctgaggagat tagaaagctt gctattaatt ctggaaaata ttctaaaacc attaaagatg aacttaaaac ggtcgacagc attattgcag taattaattc agagattgat acaatttata aaaatttcat agacattcaa gataatgtgg acaacaattt ttcaagacac gagaaagtag atcttactct tgctaagcat tttaaagaaa ttggcgagtt taaagaaagg tatttgtctc acgatactaa gatcagagat gctaagaata tgtataaaga aatatttaat aatcattatt ttattagtgg caagtttaac aactttagtc aagatttaaa agagtttaaa gtttctaaga tgaatttaga tgcggtaagt tctcttcaag aatattcatc tttagtaaag tcttctaagg ataagatatt aaagacaaag gaattgattc aaaagattaa tgatgagatt aaagatattc ttttttag
Details for BobuA.18975.a.B3.GE39002
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN HKNEYLDRFK FDSKLFLNSV SAVIKAKYSE SSRFLEELIR DSYRFGILVN SSKSFLLSSS LKLGDSLDEN SDLFLRSREF NSIDKIFKTI PLAEDSLEGI FYIPIGKNVL ISNSNFSSLG LKDVRLDPIY SVPVEKNSKY YSRYMQIDGK IYSIISFPVR DSVSTLGVIG ILICFDESLD IIENQLYSSL KFGSKNYNFF MLDRNYMPIF SNLNNLQAKS FSTAYSENFL SKVIAYAKKD SSSSQYTFNY ERDFYSLNFV KTDDFLTQGL ILNVNSXXXM FKSN
Validated NT Sequence
ttactcactt aatttgattt aaacatnnnn ngnnnngaat tgacatttaa aataagcccc tgagtcaaaa aatcatcggt ttttacaaag tttaaagaat aaaaatctct ttcataatta aaagtgtact gagagctaga agaatctttt ttagcataag ctataacttt actcaaaaaa ttctcactat aagctgtaga aaaagatttg gcctgaagat tattaaggtt tgaaaaaatg ggcatgtaat ttctgtcaag cataaaaaaa ttataatttt tactaccaaa tttaagagaa gaatacaact gattttcaat aatatctaac gactcatcaa agcatattaa aatccctatc acacccaatg ttgaaacaga atctctaact ggaaaactta ttatagaata aattttccca tctatttgca tatatcttga ataatactta gaattttttt ctacaggaac agaataaatt ggatccaatc taacatcttt taagcctaaa gatgaaaaat ttgaatttga tattaaaaca tttttcccta taggaatata aaatatacct tcaagtgaat cttctgctaa aggaatagtt ttaaaaattt tatctataga gttaaattcc cttgacctta aaaacaaatc actattttca tccaaactat cacctaattt taaacttgaa gacaaaagaa agctttttga agaattcacc aatatcccaa acctatagct atctcttata agctcctcaa gaaacctaga ggattccgaa tatttggctt taataacagc agacacactg tttaaaaaca acttagaatc aaatttaaag cgatccaaat attcattttt atgattcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHNH KNEYLDRFKF DSKLFLNSVS AVIKAKYSES SRFLEELIRD SYRFGILVNS SKSFLLSSSL KLGDSLDENS DLFLRSREFN SIDKIFKTIP LAEDSLEGIF YIPIGKNVLI SNSNFSSLGL KDVRLDPIYS VPVEKNSKYY SRYMQIDGKI YSIISFPVRD SVSTLGVIGI LICFDESLDI IENQLYSSLK FGSKNYNFFM LDRNYMPIFS NLNNLQAKSF STAYSENFLS KVIAYAKKDS SSSQYTFNYE RDFYSLNFVK TDDFLTQGLI LNVNSIPIMF KSN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatcata aaaatgaata tttggatcgc tttaaatttg attctaagtt gtttttaaac agtgtgtctg ctgttattaa agccaaatat tcggaatcct ctaggtttct tgaggagctt ataagagata gctataggtt tgggatattg gtgaattctt caaaaagctt tcttttgtct tcaagtttaa aattaggtga tagtttggat gaaaatagtg atttgttttt aaggtcaagg gaatttaact ctatagataa aatttttaaa actattcctt tagcagaaga ttcacttgaa ggtatatttt atattcctat agggaaaaat gttttaatat caaattcaaa tttttcatct ttaggcttaa aagatgttag attggatcca atttattctg ttcctgtaga aaaaaattct aagtattatt caagatatat gcaaatagat gggaaaattt attctataat aagttttcca gttagagatt ctgtttcaac attgggtgtg atagggattt taatatgctt tgatgagtcg ttagatatta ttgaaaatca gttgtattct tctcttaaat ttggtagtaa aaattataat ttttttatgc ttgacagaaa ttacatgccc attttttcaa accttaataa tcttcaggcc aaatcttttt ctacagctta tagtgagaat tttttgagta aagttatagc ttatgctaaa aaagattctt ctagctctca gtacactttt aattatgaaa gagattttta ttctttaaac tttgtaaaaa ccgatgattt tttgactcag gggcttattt taaatgtcaa ttccattcct attatgttta aatcaaattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat