BobuA.18974.a

Outer surface protein C (PC) (BB_B19)

CENTER ID: BobuA.18974.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18974.a.B2.GE39000 mature protein 23 210
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1608
RefSeq: NP_047005.1
RefSeq: WP_010890595.1
UniProt: Q07337

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKNTLSAIL MTLFLFISCN NSGKDGNTSA NSADESVKGP NLTEISKKIT DSNAVLLAVK EVEALLSSID EIAAKAIGKK IHQNNGLDTE NNHNGSLLAG AYAISTLIKQ KLDGLKNEGL KEKIDAAKKC SETFTNKLKE KHTDLGKEGV TDADAKEAIL KTNGTKTKGA EELGKLFESV EVLSKAAKEM LANSVKELTS PVVAESPKKP
NT Sequence
atgaaaaaga atacattaag tgcaatatta atgactttat ttttatttat atcttgtaat aattcaggga aagatgggaa tacatctgca aattctgctg atgagtctgt taaagggcct aatcttacag aaataagtaa aaaaattacg gattctaatg cggttttact tgctgtgaaa gaggttgaag cgttgctgtc atctatagat gaaattgctg ctaaagctat tggtaaaaaa atacaccaaa ataatggttt ggataccgaa aataatcaca atggatcatt gttagcggga gcttatgcaa tatcaaccct aataaaacaa aaattagatg gattgaaaaa tgaaggatta aaggaaaaaa ttgatgcggc taagaaatgt tctgaaacat ttactaataa attaaaagaa aaacacacag atcttggtaa agaaggtgtt actgatgctg atgcaaaaga agccatttta aaaacaaatg gtactaaaac taaaggtgct gaagaacttg gaaaattatt tgaatcagta gaggtcttgt caaaagcagc taaagagatg cttgctaatt cagttaaaga gcttacaagc cctgttgtgg cagaaagtcc aaaaaaacct taa
Details for BobuA.18974.a.B2.GE39000
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 21kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG KDGNTSANSA DESVKGPNLT EISKKITDSN AVLLAEKEVE ALLSSIDEIA AKAIGKKIHQ NNGLDTENNH NGSLLAGAYA ISTLIKQKLD GLKNEGLKEK IDAAKKCSET FNNKLKEKHT DLGKEGVTDA DAKEAILKTN GTKTKGAEEL GKLFESVEVL SKAAKEMLAN SVKELTSPVV AESPKKP
Validated NT Sequence
atcttactca cttaaggttt ttttggactt tctgccacaa cagggcttgt aagctcttta actgaattag caagcatctc tttagctgct tttgacaaga cctctactga ttcaaataat tttccaagtt cttcagcacc tttagtttta gtaccatttg tttttaaaat ggcttctttt gcatcagcat cagtaacacc ttctttacca agatctgtgt gtttttcttt taatttatta ttaaatgttt cagaacattt cttagccgca tcaatttttt cctttaatcc ttcatttttc aatccatcta atttttgttt tattagggtt gatattgcat aagctcccgc taacaatgat ccattgtgat tattttcggt atccaaacca ttattttggt gtattttttt accaatagct ttagcagcaa tttcatctat agatgacagc aacgcttcaa cctctttctc agcaagtaaa accgcattag aatccgtaat ttttttactt atttctgtaa gattaggccc tttaacagac tcatcagcag aatttgcaga tgtattccca tctttcccca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHGK DGNTSANSAD ESVKGPNLTE ISKKITDSNA VLLAVKEVEA LLSSIDEIAA KAIGKKIHQN NGLDTENNHN GSLLAGAYAI STLIKQKLDG LKNEGLKEKI DAAKKCSETF TNKLKEKHTD LGKEGVTDAD AKEAILKTNG TKTKGAEELG KLFESVEVLS KAAKEMLANS VKELTSPVVA ESPKKP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggaaag atgggaatac atctgcaaat tctgctgatg agtctgttaa agggcctaat cttacagaaa taagtaaaaa aattacggat tctaatgcgg ttttacttgc tgtgaaagag gttgaagcgt tgctgtcatc tatagatgaa attgctgcta aagctattgg taaaaaaata caccaaaata atggtttgga taccgaaaat aatcacaatg gatcattgtt agcgggagct tatgcaatat caaccctaat aaaacaaaaa ttagatggat tgaaaaatga aggattaaag gaaaaaattg atgcggctaa gaaatgttct gaaacattta ctaataaatt aaaagaaaaa cacacagatc ttggtaaaga aggtgttact gatgctgatg caaaagaagc cattttaaaa acaaatggta ctaaaactaa aggtgctgaa gaacttggaa aattatttga atcagtagag gtcttgtcaa aagcagctaa agagatgctt gctaattcag ttaaagagct tacaagccct gttgtggcag aaagtccaaa aaaaccttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at