BobuA.18967.a
Outer surface protein (BB_A66)
| CENTER ID: | BobuA.18967.a |
| ORGANISM: | Borrelia burgdorferi B31 |
| ASSOCIATED DISEASE: | Lyme disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.18967.a.B2.GE38993 | mature protein | 24 | 411 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5IZT
DEPOSITED: 3/25/2016
DETERMINATION: XRay
CLONE: BobuA.18967.a.B2.GE38993
PROTEIN: BobuA.18967.a.B2.PW37767
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|224326.49.peg.1579 |
| RefSeq: | NP_045739.1 |
| UniProt: | O50955 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIKPLIQLK LLGLFLFSCT IDANLNEDYK NKVKGILNKA ADDQETTSAD TNSNAAKNIP IADNDKVAAE LKKQSQAAKT VAAAPNKGSQ NQPQTTPNKG SQNQQAAPSP QLQSLSFSAD LSNLPKTTAA RAASLTKQRI PIQAVTTVPG NTRTFNSRNS GLPTFALNYS FSQPTRQQTN SSSAVQTTTS SGSKLQTLKN ELIRAISEEK NKTQNNFGFR ETYDQFKMKD SAFELLDVIS SAKVYDRSYA PQLNSNTPEA ENERNKFYAL MDFDQYKIEQ FGSIMEALYN ENQNHSLIRE LMISGLGTQI SFELALEEIN KKIEIFNQDY LNAKINSFDF TMKLKELKSK LNQILDKRKE WSRQADGLIA NASSNSSLSD SKSLAEYIKK RYLDNMQNAR QSVLEAYISI M
NT Sequence
ttgaaaatca aaccattaat acaattaaag cttttgggat tgtttttatt ttcttgcacg attgatgcca atctaaacga agattataaa aacaaagtaa aaggaatact taataaggca gcagatgatc aagaaacaac aagtgctgat acaaactcaa atgctgcaaa aaatatacca attgctgaca atgacaaagt agcagcagag ctaaaaaaac aatcgcaagc ggcaaaaaca gtagcagcag cgccaaataa aggatcgcaa aatcaaccac aaacaacacc aaataaagga tcgcaaaatc aacaagcagc accaagccca caattgcaaa gcttaagttt tagcgcagat ctgtcaaatc tgccaaaaac aacagcagca agagctgcat cactaacaaa acaaagaata cctattcaag ccgttacaac cgtacccgga aatacaagaa cttttaacag cagaaatagc gggctgccaa catttgcctt aaactacagc tttagtcaac caacaagaca gcaaacaaat tcaagctctg ctgtacaaac aacaacatct tctggcagta aattacaaac attaaaaaac gaacttatta gagcaatttc tgaagaaaaa aataaaacac aaaacaactt tggatttaga gaaacttacg atcaattcaa aatgaaagat tctgcatttg aactgctaga tgttatttca agtgctaaag tttatgacag aagttacgcg ccacagctta actctaacac accagaagca gaaaatgaaa gaaataaatt ctatgcgctt atggattttg atcaatacaa aattgaacaa tttggatcaa taatggaagc tctttacaat gagaatcaaa atcacagctt aataagagaa ttaatgatat cagggcttgg aacacaaatt tctttcgaac ttgcactaga agaaataaat aaaaaaattg aaatctttaa ccaggattac ttaaacgcta aaatcaacag ttttgatttc accatgaagc taaaagagct taaatcaaaa ctaaatcaaa tattagataa aagaaaagaa tggtcaagac aagctgatgg gcttattgct aatgctagct ctaattcaag tttaagcgat tccaaatcct tagcagagta cattaaaaaa agatacctag ataatatgca aaatgctcgc cagtctgttc ttgaagcata cattagtata atgtaa
Details for BobuA.18967.a.B2.GE38993
| HARVESTED ON: | 3/4/2015 |
| SEQUENCED ON: | 3/4/2015 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMN LNEDYKNKVK GILNKAADDQ ETTSADTNSN AAKNIPTADK VAAELKKQSQ AAKTVAAAPN KGSQNQPQTT PNKGSQNQQA APSPQLQSLS FSADLSNLPK TTAARAASLT KQRIPIQAVT TVPGNTRTFN SRNSGLPTFA LNYSFSQPTR QQTNSSFAVQ TTTSSGSKLQ TLKNELIRAI SEEKNKTQNN FGFRETYDQF KMKDSAFELL DVISSAKVYD RSYAPQLNSN TPEAENERNK FYALMDFDQY KIEQFGSIME TLYNENQNHS LIRELMISGL GTQISFELAL EEINKKIEIF NQDYLNAKIN SFDFTMKLKE LKSKLNQILD KRKEWSRQAD GLIANASSNS SLSDSKSLAE YIKKRYLDNM QNARQSV
Validated NT Sequence
agaacagact ggcgagcatt ttgcatatta tctaggtatc tttttttaat gtactctgct aaggatttgg aatcgcttaa acttgaatta gagctagcat tagcaataag cccatcagct tgtcttgacc attcttttct tttatctaat atttgattta gttttgattt aagctctttt agcttcatgg tgaaatcaaa actgttgatt ttagcgttta agtaatcctg gttaaagatt tcaatttttt tatttatttc ttctagtgca agttcgaaag aaatttgtgt tccaagccct gatatcatta attctcttat taagctgtga ttttgattct cattgtaaag agtttccatt attgatccaa attgttcaat tttgtattga tcaaaatcca taagcgcata gaatttattt ctttcatttt ctgcttctgg tgtgttagag ttaagctgtg gcgcgtaact tctgtcataa actttagcac ttgaaataac atctagcagt tcaaatgcag aatctttcat tttgaattga tcgtaagttt ctctaaatcc aaagttgttt tgtgttttat ttttttcttc agaaattgct ctaataagtt cgttttttaa tgtttgtaat ttactgccag aagatgttgt tgtttgtaca gcaaagcttg aatttgtttg ctgtcttgtt ggttgactaa agctgtagtt taaggcaaat gttggcagcc cgctatttct gctgttaaaa gttcttgtat ttccgggtac ggttgtaacg gcttgaatag gtattctttg ttttgttagt gatgcagctc ttgctgctgt tgtttttggc agatttgaca gatctgcgct aaaacttagg ctttgcaatt gtgggcttgg tgctgcttgt tgattttgcg atcctttatt tggtgttgtt tgtggttgat tttgcgatcc tttatttggc gctgctgcta ctgtttttgc cgcttgcgat tgttttttta gctctgctgc tactttgtca gcagttggta tattttttgc agcatttgag tttgtatcag cacttgttgt ttcttgatca tctgctgcct tattaagtat tccttttact ttgtttttat aatcttcgtt tagattcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHNL NEDYKNKVKG ILNKAADDQE TTSADTNSNA AKNIPIADND KVAAELKKQS QAAKTVAAAP NKGSQNQPQT TPNKGSQNQQ AAPSPQLQSL SFSADLSNLP KTTAARAASL TKQRIPIQAV TTVPGNTRTF NSRNSGLPTF ALNYSFSQPT RQQTNSSSAV QTTTSSGSKL QTLKNELIRA ISEEKNKTQN NFGFRETYDQ FKMKDSAFEL LDVISSAKVY DRSYAPQLNS NTPEAENERN KFYALMDFDQ YKIEQFGSIM EALYNENQNH SLIRELMISG LGTQISFELA LEEINKKIEI FNQDYLNAKI NSFDFTMKLK ELKSKLNQIL DKRKEWSRQA DGLIANASSN SSLSDSKSLA EYIKKRYLDN MQNARQSVLE AYISIM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatctaa acgaagatta taaaaacaaa gtaaaaggaa tacttaataa ggcagcagat gatcaagaaa caacaagtgc tgatacaaac tcaaatgctg caaaaaatat accaattgct gacaatgaca aagtagcagc agagctaaaa aaacaatcgc aagcggcaaa aacagtagca gcagcgccaa ataaaggatc gcaaaatcaa ccacaaacaa caccaaataa aggatcgcaa aatcaacaag cagcaccaag cccacaattg caaagcttaa gttttagcgc agatctgtca aatctgccaa aaacaacagc agcaagagct gcatcactaa caaaacaaag aatacctatt caagccgtta caaccgtacc cggaaataca agaactttta acagcagaaa tagcgggctg ccaacatttg ccttaaacta cagctttagt caaccaacaa gacagcaaac aaattcaagc tctgctgtac aaacaacaac atcttctggc agtaaattac aaacattaaa aaacgaactt attagagcaa tttctgaaga aaaaaataaa acacaaaaca actttggatt tagagaaact tacgatcaat tcaaaatgaa agattctgca tttgaactgc tagatgttat ttcaagtgct aaagtttatg acagaagtta cgcgccacag cttaactcta acacaccaga agcagaaaat gaaagaaata aattctatgc gcttatggat tttgatcaat acaaaattga acaatttgga tcaataatgg aagctcttta caatgagaat caaaatcaca gcttaataag agaattaatg atatcagggc ttggaacaca aatttctttc gaacttgcac tagaagaaat aaataaaaaa attgaaatct ttaaccagga ttacttaaac gctaaaatca acagttttga tttcaccatg aagctaaaag agcttaaatc aaaactaaat caaatattag ataaaagaaa agaatggtca agacaagctg atgggcttat tgctaatgct agctctaatt caagtttaag cgattccaaa tccttagcag agtacattaa aaaaagatac ctagataata tgcaaaatgc tcgccagtct gttcttgaag catacattag tataatgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at