BobuA.18963.a

Aminoacyl-histidine dipeptidase (BB_0608)

CENTER ID: BobuA.18963.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18963.a.B1.GE38989 full length 1 476
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.999
RefSeq: NP_212742.1
RefSeq: WP_010889778.1
UniProt: O51553

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIVIDSFKE ISKIPRCSKN IKGIINFIKQ RAKKFGYSFK EDRVGNIVVQ IKSNNNIDMH PIILQSHVDM VCEKNESSLH NFETDPIEIV EEDGYLKAVG TTLGADNGIG VAMMLGIMSE ANNFPHPDLE LLFTVDEEIG LIGALGLDSN LCSGKSLINL DGEEEGYFLV GCAGSRLVEI VFSPKYSLVT KKTKVEILFK GLKGGHSGAD IHLDLANSLK LMFFALFEIK ANLNFEIEGI FGGNSSNAIP IEAKALIFID DNDYDLLNKE LKLFVLKVKS AYSLEDEFDI IVNKREFSSV KVLDENSKNK LLNMGMGFLH GVQKVENYEN KLIKTSLNFS SLLRMQDDYI FTFLIRSLLD LDKEYVCNHL QSISDLSGAN LRVIYDDPSW QPDKNSNLLK HLQEVYKEMY LEDANVSTIH AGLETGIISS RLGGIESVTL GPWIEWPHTT RERVNISSTI RVYDFLKKSL ERFQKF
NT Sequence
atgagtattg taattgattc ttttaaagag atatcaaaaa ttccaagatg ttcaaaaaat attaaaggaa ttattaattt tattaagcag agagccaaaa aatttggtta ttcttttaaa gaagatcgtg ttggtaatat tgtagtgcag ataaaatcaa ataataatat tgatatgcat cctattattt tacaatctca tgttgatatg gtttgcgaaa agaatgaatc cagtttgcat aattttgaaa cagatccaat tgaaattgtt gaagaggatg gatatcttaa agcagtaggg accaccctcg gagctgataa tgggattgga gtagctatga tgttggggat tatgagtgaa gccaataatt ttcctcatcc cgatttagaa cttctattta ccgttgatga agaaataggt ttaataggtg ctcttggtct tgattctaat ttatgtagtg gtaaaagcct cattaatctt gatggagagg aagagggtta ttttttagtt ggttgtgcgg gatcaagact tgtagaaatt gttttttctc ctaagtatag ccttgtaacg aaaaaaacaa aggtagaaat tttgtttaaa gggcttaaag gtggccattc tggggcagat attcatttag atttggcaaa ttctttaaaa ttaatgtttt ttgctctttt tgaaattaag gcaaatctta attttgagat tgaaggcatt tttggtggaa atagtagcaa tgcaattcca attgaagcca aggctttaat ctttatagat gataatgatt atgatttatt aaataaagag cttaaactgt tcgtacttaa agttaaaagt gcatattctc ttgaagatga atttgatatt attgttaata aaagagaatt ttcatcagtt aaagttcttg atgaaaatag caaaaataag cttttaaata tgggaatggg atttttacat ggagttcaaa aagtagaaaa ttatgaaaat aagcttataa agacctcttt aaatttttca agtcttttga gaatgcaaga cgattatatt tttacttttt taataagatc tttattggat ttagacaaag agtatgtttg caatcattta caatcaataa gtgatttgtc gggagctaat ttgcgtgtaa tttatgatga tccttcttgg cagcctgata agaatagcaa tcttttaaaa catcttcaag aagtgtataa agagatgtat cttgaagatg ctaatgtttc cacaatacat gcaggtcttg aaacaggcat aatatcttct agattgggag gtatagaatc tgttaccctt gggccttgga ttgaatggcc tcatactact agggaaagag taaatatttc ttcaactatt agagtttatg attttttgaa aaaaagctta gaaagatttc aaaaatttta g
Details for BobuA.18963.a.B1.GE38989
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS IVIDSFKEIS KIPRCSKNIK GIINFIKQRA KKFGYSFKED RVGNIVVQIK SNNNIDMHPI ILQSHVDMVC EKNESSLHNF ETDPIEIVEE DGYLKAVGTT LGADNGIGVA MMLGIMSEAN NFPHPDLELL FTVDEEIGLI GALGLDSNLC SGKSLINLDG EEEGYFLVGC AGSRLVEIVF SPKYSLVTKK TKVEILFKGL KGGHSGADIH LDLANSLKLM FFALFEIKAN LNFEIEGIFG GNSSNAIPIE AKALIFIDDN DYDLLNKELK LFVLKVKSAY SLEDEFDIIV NKREFSSVKV LDENSKNKLL NMGMGFLHGV QKVENYENKL IKTSLNFSSL LRMQDDYIFT FLIRSLLDLD KEYVCNHLQS ISDLSGANLR VIYDDPSWQP DKNSNLLKHL QEVYKEMYLE DANVSTIHAG LETGIISSRL GGIESVTLGP WIEWPHTTRE RVNISSTIRV YDFLKK
Validated NT Sequence
ctttttttca aaaaatcata aactctaata gttgaagaaa tatttactct ttccctagta gtatgaggcc attcaatcca aggcccaagg gtaacagatt ctatacctcc caatctagaa gatattatgc ctgtttcaag acctgcatgt attgtggaaa cattagcatc ttcaagatac atctctttat acacttcttg aagatgtttt aaaagattgc tattcttatc aggctgccaa gaaggatcat cataaattac acgcaaatta gctcccgaca aatcacttat tgattgtaaa tgattgcaaa catactcttt gtctaaatcc aataaagatc ttattaaaaa agtaaaaata taatcgtctt gcattctcaa aagacttgaa aaatttaaag aggtctttat aagcttattt tcataatttt ctactttttg aactccatgt aaaaatccca ttcccatatt taaaagctta tttttgctat tttcatcaag aactttaact gatgaaaatt ctcttttatt aacaataata tcaaattcat cttcaagaga atatgcactt ttaactttaa gtacgaacag tttaagctct ttatttaata aatcataatc attatcatct ataaagatta aagccttggc ttcaattgga attgcattgc tactatttcc accaaaaatg ccttcaatct caaaattaag atttgcctta atttcaaaaa gagcaaaaaa cattaatttt aaagaatttg ccaaatctaa atgaatatct gccccagaat ggccaccttt aagcccttta aacaaaattt ctacctttgt ttttttcgtt acaaggctat acttaggaga aaaaacaatt tctacaagtc ttgatcccgc acaaccaact aaaaaataac cctcttcctc tccatcaaga ttaatgaggc ttttaccact acataaatta gaatcaagac caagagcacc tattaaacct atttcttcat caacggtaaa tagaagttct aaatcgggat gaggaaaatt attggcttca ctcataatcc ccaacatcat agctactcca atcccattat cagctccgag ggtggtccct actgctttaa gatatccatc ctcttcaaca atttcaattg gatctgtttc aaaattatgc aaactggatt cattcttttc gcaaaccata tcaacatgag attgtaaaat aataggatgc atatcaatat tattatttga ttttatctgc actacaatat taccaacacg atcttcttta aaagaataac caaatttttt ggctctctgc ttaataaaat taataattcc tttaatattt tttgaacatc ttggaatttt tgatatctct ttaaaagaat caattacaat actcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS IVIDSFKEIS KIPRCSKNIK GIINFIKQRA KKFGYSFKED RVGNIVVQIK SNNNIDMHPI ILQSHVDMVC EKNESSLHNF ETDPIEIVEE DGYLKAVGTT LGADNGIGVA MMLGIMSEAN NFPHPDLELL FTVDEEIGLI GALGLDSNLC SGKSLINLDG EEEGYFLVGC AGSRLVEIVF SPKYSLVTKK TKVEILFKGL KGGHSGADIH LDLANSLKLM FFALFEIKAN LNFEIEGIFG GNSSNAIPIE AKALIFIDDN DYDLLNKELK LFVLKVKSAY SLEDEFDIIV NKREFSSVKV LDENSKNKLL NMGMGFLHGV QKVENYENKL IKTSLNFSSL LRMQDDYIFT FLIRSLLDLD KEYVCNHLQS ISDLSGANLR VIYDDPSWQP DKNSNLLKHL QEVYKEMYLE DANVSTIHAG LETGIISSRL GGIESVTLGP WIEWPHTTRE RVNISSTIRV YDFLKKSLER FQKF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta ttgtaattga ttcttttaaa gagatatcaa aaattccaag atgttcaaaa aatattaaag gaattattaa ttttattaag cagagagcca aaaaatttgg ttattctttt aaagaagatc gtgttggtaa tattgtagtg cagataaaat caaataataa tattgatatg catcctatta ttttacaatc tcatgttgat atggtttgcg aaaagaatga atccagtttg cataattttg aaacagatcc aattgaaatt gttgaagagg atggatatct taaagcagta gggaccaccc tcggagctga taatgggatt ggagtagcta tgatgttggg gattatgagt gaagccaata attttcctca tcccgattta gaacttctat ttaccgttga tgaagaaata ggtttaatag gtgctcttgg tcttgattct aatttatgta gtggtaaaag cctcattaat cttgatggag aggaagaggg ttatttttta gttggttgtg cgggatcaag acttgtagaa attgtttttt ctcctaagta tagccttgta acgaaaaaaa caaaggtaga aattttgttt aaagggctta aaggtggcca ttctggggca gatattcatt tagatttggc aaattcttta aaattaatgt tttttgctct ttttgaaatt aaggcaaatc ttaattttga gattgaaggc atttttggtg gaaatagtag caatgcaatt ccaattgaag ccaaggcttt aatctttata gatgataatg attatgattt attaaataaa gagcttaaac tgttcgtact taaagttaaa agtgcatatt ctcttgaaga tgaatttgat attattgtta ataaaagaga attttcatca gttaaagttc ttgatgaaaa tagcaaaaat aagcttttaa atatgggaat gggattttta catggagttc aaaaagtaga aaattatgaa aataagctta taaagacctc tttaaatttt tcaagtcttt tgagaatgca agacgattat atttttactt ttttaataag atctttattg gatttagaca aagagtatgt ttgcaatcat ttacaatcaa taagtgattt gtcgggagct aatttgcgtg taatttatga tgatccttct tggcagcctg ataagaatag caatctttta aaacatcttc aagaagtgta taaagagatg tatcttgaag atgctaatgt ttccacaata catgcaggtc ttgaaacagg cataatatct tctagattgg gaggtataga atctgttacc cttgggcctt ggattgaatg gcctcatact actagggaaa gagtaaatat ttcttcaact attagagttt atgatttttt gaaaaaaagc ttagaaagat ttcaaaaatt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat