BobuA.18945.a

Flagellar biosynthesis protein FlhA (BB_0271)

CENTER ID: BobuA.18945.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18945.a.B2.GE38975 FlhA-like cytoplasmic domain 318 697
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.670
RefSeq: NP_212405.1
RefSeq: WP_010889723.1
UniProt: Q44909

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDARKNSIL GYLGLNNKSD LIISVGLIFV VAGFILPLPA VILDVLITVN LVISLLIILI VLYSKRSLDF SVFPTLLLVM TIFGLVLNIS STRLILTKGI NFDGQMIRTF GTFVVGSSGI QGLVIGFIIF IIIIAVQFIV ITKGATRVAE VAARFALDAL PGKQMAIDSA YSSGNLTEEE ATRQKNDLQS EVNFYGAMDG ASKFVSGNVK VGFLITLINI LGGLLVGITL QGLNFNEALN NYVSLTVGDG LVSQLPSLLI STSTGLIVTR SISKNSFGGE IFDQFTNHLG IYWIVSGFLL FLAFLPGFPT LILMFLSLSI AFLAYSLSGI RQKEEIDKKM KLEEEQASIY SDKDVSPVVP LDPLALEIGY NLVPIVDDTK TSELLDRIVK IRREIAFEFG IVVPKIRIVD NMRLEPNAYS FKLRGVEVGR GEIKLGKFLV INVGIDSGID GDLVKDPSFG LPSLWVNDDG RETAEKLGYT VVDPPSIIAT HMTELIKRHS YEILTRQDVQ NTLDVFKKDY GAIVEEVLKN FSVGEIQRVL QGLLKEQVSI RNLVTIFETI ADFTSITKDI FFLIEKCRQS VGRQITSGYL DLNSELNVIT LNPSFEQMII DSRVESNHDL ISSIDPNLKT KFIYELFKIV NEVQAEGFYP VVLSSESSRP IIKVITSREI PDLVVMSVLE VPQNIKVNVL KTVEVEE
NT Sequence
ttgttggatg ctagaaaaaa ttctatatta gggtatttgg ggcttaataa taagtctgat ttaataattt cggtcggttt gatatttgtt gttgccggtt ttattttgcc tcttcctgca gttattttgg atgttttgat tacggttaat ttagtaataa gtcttttaat tattttaatt gttctttatt ctaagagatc tctagatttc tctgtttttc ccacattact gcttgtgatg actatttttg gactcgttct taatatttct tctactaggt taattttaac caaaggtata aattttgatg ggcaaatgat aagaacattt gggacatttg ttgtgggtag ttctggaatt caaggacttg ttattggatt tataatattt ataataatca ttgctgttca atttattgta attaccaagg gagcaacaag ggtagctgaa gttgcagctc ggtttgctct tgatgcactt cctggcaagc aaatggcgat tgattctgcc tacagttctg gaaatttgac agaagaagag gctacaaggc aaaaaaacga tttacaatca gaagtcaatt tttatggtgc aatggacgga gcttctaaat ttgtatcagg aaacgttaag gttggatttt taataactct tataaatatt cttggtggtt tgttagtggg aataactttg caaggtctta actttaacga agctcttaac aattatgttt cattgacagt tggtgatggg cttgtgtctc aattgccatc tcttttaatt tcaacgtcta caggcttgat tgttactagg tcgatttcaa aaaatagttt tggcggcgag atttttgatc aattcacaaa tcatttaggc atttattgga ttgtttccgg gtttttgctt tttttagctt ttcttccggg atttccaacc ctaattctta tgtttttaag tttgtcaatt gcatttttgg cttattctct ttcaggaata agacaaaaag aagaaataga taaaaaaatg aaacttgaag aagagcaggc gtcaatttat tcagacaaag atgtttctcc tgttgttccg cttgatccat tagctcttga gattggatat aatcttgttc caatagttga tgatacaaaa acttctgagc tgcttgatcg tattgttaag ataaggcgtg agattgcatt tgaatttgga atagttgtgc ccaagattag aatagttgat aatatgcgac ttgagcccaa tgcttattct tttaaactaa gaggagttga agttgggcga ggcgagatta agcttggcaa atttttagtg ataaatgttg gaattgattc tggaatagat ggagatcttg ttaaagatcc ttcatttgga cttccttctc tttgggtaaa tgatgatggg cgagaaactg ctgaaaaatt aggatatact gttgtagatc ctccttcaat tattgctact catatgacag aacttattaa aagacattct tatgaaattt tgactcgtca agatgttcaa aatactcttg atgtttttaa aaaagattac ggagctattg ttgaagaggt tcttaagaat ttttcggttg gcgagattca aagggtttta caaggtcttt taaaagagca ggtttcaatt cgcaatttag ttacaatttt tgaaacaatt gcagatttca caagtattac taaggatata tttttcttga ttgagaaatg taggcaatcg gttggaaggc aaataactag tggatattta gatttaaatt ctgagcttaa tgtaataact ttaaacccca gttttgagca aatgataatt gattctcgtg tagaatccaa tcatgatctt ataagttcaa ttgatcctaa tttaaaaact aaatttattt atgaactttt caaaattgta aacgaagttc aagcagaagg tttttatcca gttgttctct caagcgaatc gtcaagacct ataataaaag tgataacaag tagagagatt ccagatcttg ttgttatgtc tgttttagag gttccccaaa atattaaagt taatgttctt aaaacagtag aggttgaaga ataa
Details for BobuA.18945.a.B2.GE38975
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 44kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML SIAFLAYSLS GIRQKEEIDK KMKLEEEQAS IYSDKDVSPV VPLDPLALEI GYNLVPIVDD TKTSELLDRI VKIRREIAFE FGIVVPKIRI VDNMRLEPNA YSFKLRGVEV GRGEIKLGKF LVINVGIDSG IDGDLVKDPS FGLPSLWVND DGRETAEKLG YTVVDPPSII ATHMTELIKR HSYEILTRQD VQNTLDVFKK DYGAIVEEVL KNFSVGEIQR VLQGLLKEQV SIRNLVTIFE TIADFTSITK DIFFLIEKCR QSVGRQITSG YLDLNSELNV ITLNPSFEQM IIDSRVESNH DLISSIEPNL KTKFIYELFK IVNEVQAEGF YPVVLSSESS RPIIKVITSR EIPDLVVMSV LEVPQNIKVN V
Validated NT Sequence
aagaacatta actttaatat tttggggaac ctctaaaaca gacataacaa caagatctgg aatctctcta cttgttatca cttttattat aggtcttgac gattcgcttg agagaacaac tggataaaaa ccttctgctt gaacttcgtt tacaattttg aaaagttcat aaataaattt agtttttaaa ttaggttcaa ttgaacttat aagatcatga ttggattcta cacgagaatc aattatcatt tgctcaaaac tggggtttaa agttattaca ttaagctcag aatttaaatc taaatatcca ctagttattt gccttccaac cgattgccta catttctcaa tcaagaaaaa tatatcctta gtaatacttg tgaaatctgc aattgtttca aaaattgtaa ctaaattgcg aattgaaacc tgctctttta aaagaccttg taaaaccctt tgaatctcgc caaccgaaaa attcttaaga acctcttcaa caatagctcc gtaatctttt ttaaaaacat caagagtatt ttgaacatct tgacgagtca aaatttcata agaatgtctt ttaataagtt ctgtcatatg agtagcaata attgaaggag gatctacaac agtatatcct aatttttcag cagtttctcg cccatcatca tttacccaaa gagaaggaag tccaaatgaa ggatctttaa caagatctcc atctattcca gaatcaattc caacatttat cactaaaaat ttgccaagct taatctcgcc tcgcccaact tcaactcctc ttagtttaaa agaataagca ttgggctcaa gtcgcatatt atcaactatt ctaatcttgg gcacaactat tccaaattca aatgcaatct cacgccttat cttaacaata cgatcaagca gctcagaagt ttttgtatca tcaactattg gaacaagatt atatccaatc tcaagagcta atggatcaag cggaacaaca ggagaaacat ctttgtctga ataaattgac gcctgctctt cttcaagttt cattttttta tctatttctt ctttttgtct tattcctgaa agagaataag ccaaaaatgc aattgacaac atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHLS IAFLAYSLSG IRQKEEIDKK MKLEEEQASI YSDKDVSPVV PLDPLALEIG YNLVPIVDDT KTSELLDRIV KIRREIAFEF GIVVPKIRIV DNMRLEPNAY SFKLRGVEVG RGEIKLGKFL VINVGIDSGI DGDLVKDPSF GLPSLWVNDD GRETAEKLGY TVVDPPSIIA THMTELIKRH SYEILTRQDV QNTLDVFKKD YGAIVEEVLK NFSVGEIQRV LQGLLKEQVS IRNLVTIFET IADFTSITKD IFFLIEKCRQ SVGRQITSGY LDLNSELNVI TLNPSFEQMI IDSRVESNHD LISSIDPNLK TKFIYELFKI VNEVQAEGFY PVVLSSESSR PIIKVITSRE IPDLVVMSVL EVPQNIKVNV LKTVEVEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtcaa ttgcattttt ggcttattct ctttcaggaa taagacaaaa agaagaaata gataaaaaaa tgaaacttga agaagagcag gcgtcaattt attcagacaa agatgtttct cctgttgttc cgcttgatcc attagctctt gagattggat ataatcttgt tccaatagtt gatgatacaa aaacttctga gctgcttgat cgtattgtta agataaggcg tgagattgca tttgaatttg gaatagttgt gcccaagatt agaatagttg ataatatgcg acttgagccc aatgcttatt cttttaaact aagaggagtt gaagttgggc gaggcgagat taagcttggc aaatttttag tgataaatgt tggaattgat tctggaatag atggagatct tgttaaagat ccttcatttg gacttccttc tctttgggta aatgatgatg ggcgagaaac tgctgaaaaa ttaggatata ctgttgtaga tcctccttca attattgcta ctcatatgac agaacttatt aaaagacatt cttatgaaat tttgactcgt caagatgttc aaaatactct tgatgttttt aaaaaagatt acggagctat tgttgaagag gttcttaaga atttttcggt tggcgagatt caaagggttt tacaaggtct tttaaaagag caggtttcaa ttcgcaattt agttacaatt tttgaaacaa ttgcagattt cacaagtatt actaaggata tatttttctt gattgagaaa tgtaggcaat cggttggaag gcaaataact agtggatatt tagatttaaa ttctgagctt aatgtaataa ctttaaaccc cagttttgag caaatgataa ttgattctcg tgtagaatcc aatcatgatc ttataagttc aattgatcct aatttaaaaa ctaaatttat ttatgaactt ttcaaaattg taaacgaagt tcaagcagaa ggtttttatc cagttgttct ctcaagcgaa tcgtcaagac ctataataaa agtgataaca agtagagaga ttccagatct tgttgttatg tctgttttag aggttcccca aaatattaaa gttaatgttc ttaaaacagt agaggttgaa gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat