BobuA.18931.b

Uncharacterized protein (BB_R06)

CENTER ID: BobuA.18931.b
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18931.b.B1.GE39303 full length 1 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18931.b.B1.PW37785 full length 1 319

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.93
RefSeq: NP_051253.2
UniProt: Q9S0G3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MELFDENYYA KAVANIIGEV KDPIMYKWFS PDQIEDVDLQ MGYQRTVKWD AFLNANPTTI ANEVNTISTI GFSSEVVRLN YLKLQYKFRH LKQASEKFYT SDSYLGDINN NLLPFSQAYK LASSEIIKLI NHFVLTGTVS IQKDGKNQKR LLPNMYGLLN MPHQVKEEVE SANKDKMDKI FEKIEAGLSK LELGDEFSTP MMVIVDPTTS LKLVKPYAAA QGAASSCEKW EDVLIQTIKA INNREDVYIE TSNLLKHQIL IYPLNPELIK FKPSKYMLPT PNEQVDKDST DIAHSYIDFV LGGLLATRKT ILQVHIKQS
NT Sequence
atggaattat ttgatgaaaa ttattatgca aaagctgtgg ctaatattat aggggaagtt aaagatccta taatgtataa atggttttcg cctgatcaaa ttgaagatgt tgatcttcag atgggctacc aaagaactgt aaagtgggat gcgtttttaa atgctaatcc tacaacgatt gctaatgaag ttaatactat ttcaactatt ggatttagtt ctgaagtggt aagacttaat tacttaaaat tacagtacaa atttaggcac ttaaagcagg cgtctgaaaa attttacact tcagactcat atcttggtga cattaataac aacttacttc ctttctctca agcctataag cttgcaagta gcgagattat taaacttatt aatcactttg tactaacagg cactgtttca attcaaaaag atggaaaaaa tcaaaaacgt ctacttccca atatgtatgg acttcttaat atgccacatc aagtaaaaga agaggttgaa agtgctaata aggacaaaat ggataaaata tttgaaaaaa ttgaggcggg actttcaaag ttagagctag gagacgaatt ttctacacct atgatggtaa tagttgaccc tacaacttcg ctcaaactcg taaagcccta cgcagcagca cagggtgcag caagtagctg cgaaaaatgg gaggatgttt tgattcaaac tattaaggct attaataata gagaagatgt ctacattgaa acttcaaact tgctaaaaca tcaaatactt atttacccat taaacccaga gcttattaag tttaaaccta gcaagtatat gttgcctaca ccaaatgaac aagtggataa agattcaact gatattgctc attcatacat tgattttgtt ttaggagggc tacttgctac tagaaaaact attttgcaag tacatattaa acaaagttaa
Details for BobuA.18931.b.B1.GE39303
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHME LFDENYYAKA AADIIGEVKD PIMYKWFSPD QIEDVDLQMG YQRTVKWDAF LNANPTTIAN EVNTISTIGF SSEVVRLNYL KLQYKFRHLK QASEKFYTSD SYLGDINNNL LPFSQAYKLA SSEIIKLINH FVLTGTVSTQ KDGKNQKRLL PNMYGLLNMP HQVKEEVASG DKDKMDKIFE KIEAGLSKLE LGDEFSTPMM VIVDPTTSLK LVKPYAAAQG AASSCEKWED VLIQTIKAIN NREDVYIETS NLLKHQILIY PLNPELIKFK PSKYMLPTPN EQVDKDSTDI AHSYIDFVLG GLLATRKTXX ASTY
Validated NT Sequence
tcttactcac ttaactttgt ttaatatgta cttgcnnnnn nagtttttct agtagcaagt aaccctccta aaacaaaatc aatgtatgaa tgagcaatat cagttgaatc tttatccact tgttcatttg gtgtaggtaa catatacttg ctaggtttaa acttaataag ctctgggttt aatgggtaaa taagtatttg atgttttagc aagtttgaag tttcaatgta gacatcttct ctattattaa tagccttgat agtttgaatc aaaacatcct cccatttttc gcagctactt gctgcaccct gtgctgctgc gtatggcttt acgagtttga gcgaagttgt agggtcaact attaccatca taggtgtaga aaattcgtct cctagctcta actttgaaag tcccgcctca attttttcaa atattttatc cattttatct ttatcaccac tagcaacttc ttcttttact tgatgtggca tattaagaag tccatacata ttgggaagta gacgtttttg attttttcca tctttttgag ttgaaacagt gcctgttagt acaaagtgat taataagttt aataatctcg ctacttgcaa gcttataggc ttgagagaaa ggaagtaaat tgttattaat gtcaccaaga tatgagtctg aagtgtaaaa tttttcagac gcctgcttta agtgcctaaa tttgtactgt aattttaagt aattaagtct taccacttca gaactaaatc caatagttga aatagtatta acctcattag caatcgttgt aggattagca tttaaaaacg catcccactt tacagttctt tggtagccca tctgaagatc aacatcttca atttgatcag gcgaaaacca tttatacatt ataggatctt taacttctcc tataatatct gccgcagctt ttgcataata attttcatca aataattcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHME LFDENYYAKA VANIIGEVKD PIMYKWFSPD QIEDVDLQMG YQRTVKWDAF LNANPTTIAN EVNTISTIGF SSEVVRLNYL KLQYKFRHLK QASEKFYTSD SYLGDINNNL LPFSQAYKLA SSEIIKLINH FVLTGTVSIQ KDGKNQKRLL PNMYGLLNMP HQVKEEVESA NKDKMDKIFE KIEAGLSKLE LGDEFSTPMM VIVDPTTSLK LVKPYAAAQG AASSCEKWED VLIQTIKAIN NREDVYIETS NLLKHQILIY PLNPELIKFK PSKYMLPTPN EQVDKDSTDI AHSYIDFVLG GLLATRKTIL QVHIKQS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat tatttgatga aaattattat gcaaaagctg tggctaatat tataggggaa gttaaagatc ctataatgta taaatggttt tcgcctgatc aaattgaaga tgttgatctt cagatgggct accaaagaac tgtaaagtgg gatgcgtttt taaatgctaa tcctacaacg attgctaatg aagttaatac tatttcaact attggattta gttctgaagt ggtaagactt aattacttaa aattacagta caaatttagg cacttaaagc aggcgtctga aaaattttac acttcagact catatcttgg tgacattaat aacaacttac ttcctttctc tcaagcctat aagcttgcaa gtagcgagat tattaaactt attaatcact ttgtactaac aggcactgtt tcaattcaaa aagatggaaa aaatcaaaaa cgtctacttc ccaatatgta tggacttctt aatatgccac atcaagtaaa agaagaggtt gaaagtgcta ataaggacaa aatggataaa atatttgaaa aaattgaggc gggactttca aagttagagc taggagacga attttctaca cctatgatgg taatagttga ccctacaact tcgctcaaac tcgtaaagcc ctacgcagca gcacagggtg cagcaagtag ctgcgaaaaa tgggaggatg ttttgattca aactattaag gctattaata atagagaaga tgtctacatt gaaacttcaa acttgctaaa acatcaaata cttatttacc cattaaaccc agagcttatt aagtttaaac ctagcaagta tatgttgcct acaccaaatg aacaagtgga taaagattca actgatattg ctcattcata cattgatttt gttttaggag ggctacttgc tactagaaaa actattttgc aagtacatat taaacaaagt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BobuA.18931.b.B1.PW37785
PURIFICATION DATe: 9/16/2015
CONCENTRATION: 5.5mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME LFDENYYAKA AADIIGEVKD PIMYKWFSPD QIEDVDLQMG YQRTVKWDAF LNANPTTIAN EVNTISTIGF SSEVVRLNYL KLQYKFRHLK QASEKFYTSD SYLGDINNNL LPFSQAYKLA SSEIIKLINH FVLTGTVSTQ KDGKNQKRLL PNMYGLLNMP HQVKEEVASG DKDKMDKIFE KIEAGLSKLE LGDEFSTPMM VIVDPTTSLK LVKPYAAAQG AASSCEKWED VLIQTIKAIN NREDVYIETS NLLKHQILIY PLNPELIKFK PSKYMLPTPN EQVDKDSTDI AHSYIDFVLG GLLATRKTXX ASTY
Validated NT Sequence
tcttactcac ttaactttgt ttaatatgta cttgcnnnnn nagtttttct agtagcaagt aaccctccta aaacaaaatc aatgtatgaa tgagcaatat cagttgaatc tttatccact tgttcatttg gtgtaggtaa catatacttg ctaggtttaa acttaataag ctctgggttt aatgggtaaa taagtatttg atgttttagc aagtttgaag tttcaatgta gacatcttct ctattattaa tagccttgat agtttgaatc aaaacatcct cccatttttc gcagctactt gctgcaccct gtgctgctgc gtatggcttt acgagtttga gcgaagttgt agggtcaact attaccatca taggtgtaga aaattcgtct cctagctcta actttgaaag tcccgcctca attttttcaa atattttatc cattttatct ttatcaccac tagcaacttc ttcttttact tgatgtggca tattaagaag tccatacata ttgggaagta gacgtttttg attttttcca tctttttgag ttgaaacagt gcctgttagt acaaagtgat taataagttt aataatctcg ctacttgcaa gcttataggc ttgagagaaa ggaagtaaat tgttattaat gtcaccaaga tatgagtctg aagtgtaaaa tttttcagac gcctgcttta agtgcctaaa tttgtactgt aattttaagt aattaagtct taccacttca gaactaaatc caatagttga aatagtatta acctcattag caatcgttgt aggattagca tttaaaaacg catcccactt tacagttctt tggtagccca tctgaagatc aacatcttca atttgatcag gcgaaaacca tttatacatt ataggatctt taacttctcc tataatatct gccgcagctt ttgcataata attttcatca aataattcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHME LFDENYYAKA VANIIGEVKD PIMYKWFSPD QIEDVDLQMG YQRTVKWDAF LNANPTTIAN EVNTISTIGF SSEVVRLNYL KLQYKFRHLK QASEKFYTSD SYLGDINNNL LPFSQAYKLA SSEIIKLINH FVLTGTVSIQ KDGKNQKRLL PNMYGLLNMP HQVKEEVESA NKDKMDKIFE KIEAGLSKLE LGDEFSTPMM VIVDPTTSLK LVKPYAAAQG AASSCEKWED VLIQTIKAIN NREDVYIETS NLLKHQILIY PLNPELIKFK PSKYMLPTPN EQVDKDSTDI AHSYIDFVLG GLLATRKTIL QVHIKQS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAT TATTTGATGA AAATTATTAT GCAAAAGCTG TGGCTAATAT TATAGGGGAA GTTAAAGATC CTATAATGTA TAAATGGTTT TCGCCTGATC AAATTGAAGA TGTTGATCTT CAGATGGGCT ACCAAAGAAC TGTAAAGTGG GATGCGTTTT TAAATGCTAA TCCTACAACG ATTGCTAATG AAGTTAATAC TATTTCAACT ATTGGATTTA GTTCTGAAGT GGTAAGACTT AATTACTTAA AATTACAGTA CAAATTTAGG CACTTAAAGC AGGCGTCTGA AAAATTTTAC ACTTCAGACT CATATCTTGG TGACATTAAT AACAACTTAC TTCCTTTCTC TCAAGCCTAT AAGCTTGCAA GTAGCGAGAT TATTAAACTT ATTAATCACT TTGTACTAAC AGGCACTGTT TCAATTCAAA AAGATGGAAA AAATCAAAAA CGTCTACTTC CCAATATGTA TGGACTTCTT AATATGCCAC ATCAAGTAAA AGAAGAGGTT GAAAGTGCTA ATAAGGACAA AATGGATAAA ATATTTGAAA AAATTGAGGC GGGACTTTCA AAGTTAGAGC TAGGAGACGA ATTTTCTACA CCTATGATGG TAATAGTTGA CCCTACAACT TCGCTCAAAC TCGTAAAGCC CTACGCAGCA GCACAGGGTG CAGCAAGTAG CTGCGAAAAA TGGGAGGATG TTTTGATTCA AACTATTAAG GCTATTAATA ATAGAGAAGA TGTCTACATT GAAACTTCAA ACTTGCTAAA ACATCAAATA CTTATTTACC CATTAAACCC AGAGCTTATT AAGTTTAAAC CTAGCAAGTA TATGTTGCCT ACACCAAATG AACAAGTGGA TAAAGATTCA ACTGATATTG CTCATTCATA CATTGATTTT GTTTTAGGAG GGCTACTTGC TACTAGAAAA ACTATTTTGC AAGTACATAT TAAACAAAGT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT