SSGCID: Target Page

BobuA.18924.a

Putative ankyrin repeat protein (BB_B28)

CENTER ID:	BobuA.18924.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18924.a.B2.GE38907	soluble domain		64	414

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18924.a.B2.PS02415	soluble domain		64	414

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.1615
RefSeq:	NP_047014.1
UniProt:	O50999

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYYVLSKIF LYSGYLVIGF LSFTIFNKNL RNKIRDKLKN LYFLYYLTFF TLFIISSNLS YYFTEKQLLE DFNIFEKEFF EIHKINEQFF QKYLLNFPVP IRMELMSKFD PLYTVFNASF EKYAKNIGKS SYEIQTNYKN YIRTTNSEIK EKLEQIKENI TPIYNKYKLP ILNGENTEIS IDENGNIIPV IKNTNGQITE LLFYDQNYNL IPFKKFESYK VRFDLIPENK NINFKELINV YYLDEKNIIT PIEYYKNNID MSPYYIDLQE NKDDFLKAIK IKKEYGLYIE KKKQLQNLTE NDKLDDFKEF LLKNNNIFSL NTIFSNGNPI FTYAINVKAK SIINYLITKE FNINLTNQNS QTALHSAIIQ KYDLNFIKSL IEKGANPNIR DGDNKLPIDY SDKTSEIYKY LIGI

NT Sequence

atgagttatt atgtgctaag caaaatattt ctatattctg ggtaccttgt tattggattt ttatctttta caattttcaa taaaaattta agaaataaaa tcagagataa attaaaaaat ttatactttt tatattattt aacttttttt actcttttta ttattagttc aaatttatct tattacttca ccgaaaagca attattagaa gattttaata ttttcgaaaa agaatttttc gaaatacata aaataaacga acaatttttt caaaaatacc tactaaattt tccagtacca ataagaatgg aattgatgtc aaaatttgat ccactatata cagtgtttaa tgctagtttt gagaaatatg ctaaaaacat tggaaaaagt tcttatgaaa ttcaaacaaa ttataaaaat tatattagga caacaaattc agaaattaaa gaaaaactag aacaaataaa agaaaatatt acccctattt ataataaata taaattacct attctaaatg gagaaaatac agaaataagt attgatgaga atggaaatat tattcctgtt ataaaaaata caaacggaca aataacagaa ttactgtttt acgatcaaaa ttataattta atccctttta aaaaatttga aagctataaa gttagatttg atctaatccc agaaaataaa aacataaact tcaaagaact aataaacgtt tactatcttg atgaaaaaaa tattatcact cccatagaat attataaaaa taatatagac atgagccctt attacataga cttgcaagaa aataaagatg attttcttaa ggcaataaaa atcaaaaaag aatatggttt atacattgag aaaaaaaagc aactacaaaa tttaactgaa aatgataaac ttgatgattt taaagaattt ttattaaaaa ataataatat tttttcacta aatacaatat tttccaacgg caatccaata tttacttatg ccataaatgt aaaagcaaaa agtattataa attatttaat aacaaaagaa tttaatatta atttaacaaa tcaaaattct caaacagctc ttcatagtgc cataattcaa aaatatgatt taaattttat taaatcactt atcgaaaaag gcgctaatcc aaatatcaga gatggcgaca ataaactgcc catagattac tctgacaaaa ctagtgaaat ctataaatat ttaatcggta tttaa

Details for BobuA.18924.a.B2.GE38907

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	43kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMT EKQLLEDFNI FEKEFFEIHK INEQFFQKYL LNFPVPIRME LMSKFDPLYT VFNASFEKYA KNIGKSSYEI QTNYKNYIRT TNSEIKEKLE QIKENITPIY NKYKLPILNG ENTEISIDEK GNIIPVIKNT NGQITELLFY DQNYNLIPFK KFESYKVRFD LIPENKNINF KELINVYYLD EKNIITPIEY YKNNIDMSPY YIDLQENKDD FLKAIKIKKE YGLYIEKKKQ LQNLTENDKL DDFKEFLLKN NNIFSLNTIF SNGNPIFTYA INVKAKSIIN YLITKEFNIN LTNQNSQTAL HSAIIQKYDL NFIKSLIEKG ANPNIRDGDN KLPIDYSDKT SE

Validated NT Sequence

gatttcacta gttttgtcag agtaatctat gggcagttta ttgtcgccat ctctgatatt tggattagcg cctttttcga taagtgattt aataaaattt aaatcatatt tttgaattat ggcactatga agagctgttt gagaattttg atttgttaaa ttaatattaa attcttttgt tattaaataa tttataatac tttttgcttt tacatttatg gcataagtaa atattggatt gccgttggaa aatattgtat ttagtgaaaa aatattatta ttttttaata aaaattcttt aaaatcatca agtttatcat tttcagttaa attttgtagt tgcttttttt tctcaatgta taaaccatat tcttttttga tttttattgc cttaagaaaa tcatctttat tttcttgcaa gtctatgtaa taagggctca tgtctatatt atttttataa tattctatgg gagtgataat atttttttca tcaagatagt aaacgtttat tagttctttg aagtttatgt ttttattttc tgggattaga tcaaatctaa ctttatagct ttcaaatttt ttaaaaggga ttaaattata attttgatcg taaaacagta attctgttat ttgtccgttt gtatttttta taacaggaat aatatttcct ttctcatcaa tacttatttc tgtattttct ccatttagaa taggtaattt atatttatta taaatagggg taatattttc ttttatttgt tctagttttt ctttaatttc tgaatttgtt gtcctaatat aatttttata atttgtttga atttcataag aactttttcc aatgttttta gcatatttct caaaactagc attaaacact gtatatagtg gatcaaattt tgacatcaat tccattctta ttggtactgg aaaatttagt aggtattttt gaaaaaattg ttcgtttatt ttatgtattt cgaaaaattc tttttcgaaa atattaaaat cttctaataa ttgcttttcg gtcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHTE KQLLEDFNIF EKEFFEIHKI NEQFFQKYLL NFPVPIRMEL MSKFDPLYTV FNASFEKYAK NIGKSSYEIQ TNYKNYIRTT NSEIKEKLEQ IKENITPIYN KYKLPILNGE NTEISIDENG NIIPVIKNTN GQITELLFYD QNYNLIPFKK FESYKVRFDL IPENKNINFK ELINVYYLDE KNIITPIEYY KNNIDMSPYY IDLQENKDDF LKAIKIKKEY GLYIEKKKQL QNLTENDKLD DFKEFLLKNN NIFSLNTIFS NGNPIFTYAI NVKAKSIINY LITKEFNINL TNQNSQTALH SAIIQKYDLN FIKSLIEKGA NPNIRDGDNK LPIDYSDKTS EIYKYLIGI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataccgaaa agcaattatt agaagatttt aatattttcg aaaaagaatt tttcgaaata cataaaataa acgaacaatt ttttcaaaaa tacctactaa attttccagt accaataaga atggaattga tgtcaaaatt tgatccacta tatacagtgt ttaatgctag ttttgagaaa tatgctaaaa acattggaaa aagttcttat gaaattcaaa caaattataa aaattatatt aggacaacaa attcagaaat taaagaaaaa ctagaacaaa taaaagaaaa tattacccct atttataata aatataaatt acctattcta aatggagaaa atacagaaat aagtattgat gagaatggaa atattattcc tgttataaaa aatacaaacg gacaaataac agaattactg ttttacgatc aaaattataa tttaatccct tttaaaaaat ttgaaagcta taaagttaga tttgatctaa tcccagaaaa taaaaacata aacttcaaag aactaataaa cgtttactat cttgatgaaa aaaatattat cactcccata gaatattata aaaataatat agacatgagc ccttattaca tagacttgca agaaaataaa gatgattttc ttaaggcaat aaaaatcaaa aaagaatatg gtttatacat tgagaaaaaa aagcaactac aaaatttaac tgaaaatgat aaacttgatg attttaaaga atttttatta aaaaataata atattttttc actaaataca atattttcca acggcaatcc aatatttact tatgccataa atgtaaaagc aaaaagtatt ataaattatt taataacaaa agaatttaat attaatttaa caaatcaaaa ttctcaaaca gctcttcata gtgccataat tcaaaaatat gatttaaatt ttattaaatc acttatcgaa aaaggcgcta atccaaatat cagagatggc gacaataaac tgcccataga ttactctgac aaaactagtg aaatctataa atatttaatc ggtatttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BobuA.18924.a.B2.PS02415

PURIFICATION DATe:	6/4/2015
CONCENTRATION:	19.72mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATACCGAAA AGCAATTATT AGAAGATTTT AATATTTTCG AAAAAGAATT TTTCGAAATA CATAAAATAA ACGAACAATT TTTTCAAAAA TACCTACTAA ATTTTCCAGT ACCAATAAGA ATGGAATTGA TGTCAAAATT TGATCCACTA TATACAGTGT TTAATGCTAG TTTTGAGAAA TATGCTAAAA ACATTGGAAA AAGTTCTTAT GAAATTCAAA CAAATTATAA AAATTATATT AGGACAACAA ATTCAGAAAT TAAAGAAAAA CTAGAACAAA TAAAAGAAAA TATTACCCCT ATTTATAATA AATATAAATT ACCTATTCTA AATGGAGAAA ATACAGAAAT AAGTATTGAT GAGAATGGAA ATATTATTCC TGTTATAAAA AATACAAACG GACAAATAAC AGAATTACTG TTTTACGATC AAAATTATAA TTTAATCCCT TTTAAAAAAT TTGAAAGCTA TAAAGTTAGA TTTGATCTAA TCCCAGAAAA TAAAAACATA AACTTCAAAG AACTAATAAA CGTTTACTAT CTTGATGAAA AAAATATTAT CACTCCCATA GAATATTATA AAAATAATAT AGACATGAGC CCTTATTACA TAGACTTGCA AGAAAATAAA GATGATTTTC TTAAGGCAAT AAAAATCAAA AAAGAATATG GTTTATACAT TGAGAAAAAA AAGCAACTAC AAAATTTAAC TGAAAATGAT AAACTTGATG ATTTTAAAGA ATTTTTATTA AAAAATAATA ATATTTTTTC ACTAAATACA ATATTTTCCA ACGGCAATCC AATATTTACT TATGCCATAA ATGTAAAAGC AAAAAGTATT ATAAATTATT TAATAACAAA AGAATTTAAT ATTAATTTAA CAAATCAAAA TTCTCAAACA GCTCTTCATA GTGCCATAAT TCAAAAATAT GATTTAAATT TTATTAAATC ACTTATCGAA AAAGGCGCTA ATCCAAATAT CAGAGATGGC GACAATAAAC TGCCCATAGA TTACTCTGAC AAAACTAGTG AAATCTATAA ATATTTAATC GGTATTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T