SSGCID: Target Page

BobuA.18918.a

Outer membrane efflux protein (BB_0142)

CENTER ID:	BobuA.18918.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18918.a.B2.GE38903	mature protein		26	428

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.541
RefSeq:	NP_212276.2
UniProt:	O51167

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIFIIRGLQI KKIFLIFITV SFSFAEIIQI SPKQAVNMAL ENSLDSENAL YKENIKKLYK NNAWNVFVPN VNLSSTLSRN PSALSELERD YWGFGFGVGI NLSLSPSVLK RMQLVMLEYE SAKIERESAV RNIKLNVLKS YNQLIALKST LKVFESQIQN SKLKFEQARI AYNNGLISEI DFLDAQLKYK KSQPDLDGHI INFEKSKEIF KLLIGLDHDQ DFEIIGELPD ETIDFSLFNE ALNFNESLEI KDLNMRLKMT EQLIDSLWLD TYLPSLSLSF SYSPYKSFHE NSKGFSTGFL ASFSLNYGLT EIFPFSKSFT KIQDNNYQLK ILQNNVEGKI RNLKSSIVQK RKDIRRYKAI LDASKINVEL ANKNYQMAFN AFNSGVMDLS KLNDIELVYK QSDLKFIEDK LNYANSILEY KNLINSLD

NT Sequence

ttgattttta ttataagggg gcttcaaatt aaaaaaattt ttttaatttt tattactgtc tctttttctt ttgctgaaat tattcaaata tcccccaagc aagctgtaaa tatggcttta gaaaatagct tggattcaga aaatgctttg tacaaagaaa atataaaaaa actttataaa aataatgcat ggaatgtttt tgttccaaat gttaacctta gctctacact tagtagaaat ccttctgctt tgagtgagct tgaaagagat tattggggtt ttgggtttgg agttgggatt aatctttctt tgtccccttc tgttttaaag agaatgcaac ttgttatgtt ggaatatgaa agcgcaaaaa tagaaaggga gagtgctgtt cgtaatatca agctaaatgt tcttaagtct tacaatcaat tgatagcttt aaagagcact ttgaaagttt ttgagagtca aatacaaaat agtaaactta aatttgaaca agctagaatt gcttataaca atggactaat atcagaaata gatttccttg atgctcagct taagtataaa aaatctcaac cagatttgga tggtcatatt attaattttg aaaaatcaaa agaaattttc aaattattaa taggattaga tcatgatcaa gattttgaaa ttattggaga attgccagac gaaacaatag atttttctct atttaatgag gcattaaatt ttaatgaatc attagagatt aaggatttga atatgcgctt aaaaatgacc gagcagctca ttgattcgct ttggttagat acttatttgc caagcctttc tttgtcattt tcttactctc cttataaatc atttcatgaa aattccaagg gcttttcaac tggattttta gcatccttta gtttaaatta tggtttaact gaaatatttc cattttcaaa gagttttaca aaaatacaag ataacaacta tcagctaaaa atactacaaa acaatgttga aggtaaaatc aggaatttaa aatctagtat tgtgcaaaaa agaaaagata ttagaagata taaagcgatt cttgatgctt ctaaaattaa tgtagaatta gcgaataaaa attatcaaat ggcatttaat gcttttaatt ccggggtcat ggatctttct aaattaaatg atattgaact tgtttataag cagagcgatt taaagttcat tgaagataaa ttaaattatg ccaattctat acttgaatat aaaaatttaa taaattcatt agactaa

Details for BobuA.18918.a.B2.GE38903

HARVESTED ON:	1/29/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHME IIQISPKQAV NMALENSLDS ENALYKENIK KLYKNNAWNV FVPNVNLSST LSRNPSALSE LERDYWGFGF GVGINLSLSP SVLKRMQLVM LEYESAKIER ESAVRNIKLN VLKSYNQLIA LKSTLKVFES QIQNSKLKFE QARIAYNNGL ISEIDFLDAQ LKYKKSQPDL DGHIINFEKS KEIFKLLIGL DHDQDFEIIG ELPDETIDFS LFNEALNFNE SLEIKDLNMR LKMTEQLIDS LWLDTYLPSL SLSFSYSPYK SFHENSKGFS TGFLASFSLN YGLTEIFPFS KSFTKIQDNN YQLKILQNNV EGKIRNLKSS IVQKRKDIRR YKAILDASKI NVELANKNYQ MAFNAFNSGV MDLSKLNDIE LVYKQSDLKF IEDKLNYANS ILEY

Validated NT Sequence

ttatattcaa gtatagaatt ggcataattt aatttatctt caatgaactt taaatcgctc tgcttataaa caagttcaat atcatttaat ttagaaagat ccatgacccc ggaattaaaa gcattaaatg ccatttgata atttttattc gctaattcta cattaatttt agaagcatca agaatcgctt tatatcttct aatatctttt cttttttgca caatactaga ttttaaattc ctgattttac cttcaacatt gttttgtagt atttttagct gatagttgtt atcttgtatt tttgtaaaac tctttgaaaa tggaaatatt tcagttaaac cataatttaa actaaaggat gctaaaaatc cagttgaaaa gcccttggaa ttttcatgaa atgatttata aggagagtaa gaaaatgaca aagaaaggct tggcaaataa gtatctaacc aaagcgaatc aatgagctgc tcggtcattt ttaagcgcat attcaaatcc ttaatctcta atgattcatt aaaatttaat gcctcattaa atagagaaaa atctattgtt tcgtctggca attctccaat aatttcaaaa tcttgatcat gatctaatcc tattaataat ttgaaaattt cttttgattt ttcaaaatta ataatatgac catccaaatc tggttgagat tttttatact taagctgagc atcaaggaaa tctatttctg atattagtcc attgttataa gcaattctag cttgttcaaa tttaagttta ctattttgta tttgactctc aaaaactttc aaagtgctct ttaaagctat caattgattg taagacttaa gaacatttag cttgatatta cgaacagcac tctccctttc tatttttgcg ctttcatatt ccaacataac aagttgcatt ctctttaaaa cagaagggga caaagaaaga ttaatcccaa ctccaaaccc aaaaccccaa taatctcttt caagctcact caaagcagaa ggatttctac taagtgtaga gctaaggtta acatttggaa caaaaacatt ccatgcatta tttttataaa gtttttttat attttctttg tacaaagcat tttctgaatc caagctattt tctaaagcca tatttacagc ttgcttgggg gatatttgaa taatttccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHEI IQISPKQAVN MALENSLDSE NALYKENIKK LYKNNAWNVF VPNVNLSSTL SRNPSALSEL ERDYWGFGFG VGINLSLSPS VLKRMQLVML EYESAKIERE SAVRNIKLNV LKSYNQLIAL KSTLKVFESQ IQNSKLKFEQ ARIAYNNGLI SEIDFLDAQL KYKKSQPDLD GHIINFEKSK EIFKLLIGLD HDQDFEIIGE LPDETIDFSL FNEALNFNES LEIKDLNMRL KMTEQLIDSL WLDTYLPSLS LSFSYSPYKS FHENSKGFST GFLASFSLNY GLTEIFPFSK SFTKIQDNNY QLKILQNNVE GKIRNLKSSI VQKRKDIRRY KAILDASKIN VELANKNYQM AFNAFNSGVM DLSKLNDIEL VYKQSDLKFI EDKLNYANSI LEYKNLINSL D

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaatta ttcaaatatc ccccaagcaa gctgtaaata tggctttaga aaatagcttg gattcagaaa atgctttgta caaagaaaat ataaaaaaac tttataaaaa taatgcatgg aatgtttttg ttccaaatgt taaccttagc tctacactta gtagaaatcc ttctgctttg agtgagcttg aaagagatta ttggggtttt gggtttggag ttgggattaa tctttctttg tccccttctg ttttaaagag aatgcaactt gttatgttgg aatatgaaag cgcaaaaata gaaagggaga gtgctgttcg taatatcaag ctaaatgttc ttaagtctta caatcaattg atagctttaa agagcacttt gaaagttttt gagagtcaaa tacaaaatag taaacttaaa tttgaacaag ctagaattgc ttataacaat ggactaatat cagaaataga tttccttgat gctcagctta agtataaaaa atctcaacca gatttggatg gtcatattat taattttgaa aaatcaaaag aaattttcaa attattaata ggattagatc atgatcaaga ttttgaaatt attggagaat tgccagacga aacaatagat ttttctctat ttaatgaggc attaaatttt aatgaatcat tagagattaa ggatttgaat atgcgcttaa aaatgaccga gcagctcatt gattcgcttt ggttagatac ttatttgcca agcctttctt tgtcattttc ttactctcct tataaatcat ttcatgaaaa ttccaagggc ttttcaactg gatttttagc atcctttagt ttaaattatg gtttaactga aatatttcca ttttcaaaga gttttacaaa aatacaagat aacaactatc agctaaaaat actacaaaac aatgttgaag gtaaaatcag gaatttaaaa tctagtattg tgcaaaaaag aaaagatatt agaagatata aagcgattct tgatgcttct aaaattaatg tagaattagc gaataaaaat tatcaaatgg catttaatgc ttttaattcc ggggtcatgg atctttctaa attaaatgat attgaacttg tttataagca gagcgattta aagttcattg aagataaatt aaattatgcc aattctatac ttgaatataa aaatttaata aattcattag actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat