BobuA.18911.a

Flagellar biosynthetic protein FlhB (BB_0272)

CENTER ID: BobuA.18911.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18911.a.B2.GE38897 type III secretion system substrate exporter domain 229 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.671
RefSeq: NP_212406.1
RefSeq: WP_002657716.1
UniProt: Q44760

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKDEFLIKS WYIPLDFFSA DDEGRTELPT DQKKQKAREE GRVLKSTEIN TAVSLLLLFA LFFFMLSYFA LDLIAVFKEQ AIKLPEVMRM SVYTMGFAYI RSIMGYVVLF FFASLAVNFF VNIIQVGFFI TFKSLEPRWD KISFNFSRWA KNSFFSAGAF FNLFKSLLKV VIICLIYYFI IENNIGKISK LSEYTLQSGI SIVLVIAYKI CFFSVMFLAI VGVFDYLFQR SQYIESLKMT KEEVKQERKE MEGDPLLRSR IKERMRVILS TNLRVAIPQA DVVITNPEHF AVAIKWDSET MLAPKVLAKG QDEIALTIKK IARENNVPLM ENKLLARALY ANVKVNEEIP REYWEIVSKI LVRVYSITKK FN
NT Sequence
atgataaaag atgaattttt gattaaaagt tggtatattc cccttgattt tttttccgca gatgatgagg gaagaaccga attacctact gaccagaaaa agcaaaaagc aagagaagaa ggccgggtat taaagtctac tgaaattaat actgccgtta gtcttttgtt gttatttgca ttgttttttt tcatgctttc ttattttgct ttagatttaa tagctgtttt taaagagcag gccatcaagc ttcctgaagt tatgcgtatg agtgtttata ctatgggttt tgcatatatt agatctatca tggggtatgt cgttttgttt tttttcgcat ctttagctgt taattttttt gttaatatta ttcaagtagg cttttttatt acttttaaat ctttggagcc aaggtgggat aaaattagtt ttaatttttc cagatgggca aaaaattctt ttttttcagc aggggctttt ttcaatttgt ttaaaagttt gttaaaagtt gttataatat gcttgatata ttattttatt atagaaaaca atataggcaa aatttctaag ctttcggagt atacacttca atctggaatt tctattgtgt tagtgattgc ctataagata tgtttttttt cagtaatgtt tttggcaatt gtaggggtgt ttgattattt gtttcaaaga tctcagtaca ttgagagttt gaaaatgaca aaagaagagg taaagcagga aagaaaggaa atggaaggtg atcctttact tcgatctaga ataaaagaga gaatgagggt tattttaagt accaatttaa gagtagctat tcctcaagca gatgtagtaa ttacaaatcc agaacatttt gcagttgcta ttaaatggga tagcgaaaca atgttagctc caaaggtgct tgcaaaaggt caagatgaaa tagctctcac aattaaaaaa attgcaagag aaaataatgt tcctttaatg gaaaataagc tccttgcaag agctctttat gctaatgtta aggttaatga agagattcca agagaatatt gggagattgt ttcaaaaatt cttgtgagag tatattctat tactaaaaag tttaattag
Details for BobuA.18911.a.B2.GE38897
HARVESTED ON: data unavailable
SEQUENCED ON: 1/15/2015
EXPECTED MW: 18kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMQ RSQYIESLKM TKEEVKQERK EMEGDPLLRS RIKERMRVIL STNLRVAIPQ ADVVITNPEH FAVAIKWDSE TMLAPKVLAK GQDEIALTIK KIARENNVPL MENKLLARAL YANVKVNEGI PREYWEIVSK ILVRVYSITK KFIK
Validated NT Sequence
atcttactca cttaataaac tttttagtaa tagaatatac tctcacaaga atttttgaaa caatctccca atattctctt ggaatccctt cattaacctt aacattagca taaagagctc ttgcaaggag cttattttcc attaaaggaa cattattttc tcttgcaatt tttttaattg tgagagctat ttcatcttga ccttttgcaa gcacctttgg agctaacatt gtttcgctat cccatttaat agcaactgca aaatgttctg gatttgtaat tactacatct gcttgaggaa tagctactct taaattggta cttaaaataa ccctcattct ctcttttatt ctagatcgaa gtaaaggatc accttccatt tcctttcttt cctgctttac ctcttctttt gtcattttca aactctcaat gtactgagat ctttgcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHQR SQYIESLKMT KEEVKQERKE MEGDPLLRSR IKERMRVILS TNLRVAIPQA DVVITNPEHF AVAIKWDSET MLAPKVLAKG QDEIALTIKK IARENNVPLM ENKLLARALY ANVKVNEEIP REYWEIVSKI LVRVYSITKK FN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaaagat ctcagtacat tgagagtttg aaaatgacaa aagaagaggt aaagcaggaa agaaaggaaa tggaaggtga tcctttactt cgatctagaa taaaagagag aatgagggtt attttaagta ccaatttaag agtagctatt cctcaagcag atgtagtaat tacaaatcca gaacattttg cagttgctat taaatgggat agcgaaacaa tgttagctcc aaaggtgctt gcaaaaggtc aagatgaaat agctctcaca attaaaaaaa ttgcaagaga aaataatgtt cctttaatgg aaaataagct ccttgcaaga gctctttatg ctaatgttaa ggttaatgaa gagattccaa gagaatattg ggagattgtt tcaaaaattc ttgtgagagt atattctatt actaaaaagt ttaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat