BobuA.18906.a

Chemotaxis response regulator protein-glutamate methylesterase (EC 3.1.1.61) (BB_0568)

CENTER ID: BobuA.18906.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18906.a.B1.GE38893 full length 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.959
RefSeq: NP_212702.1
UniProt: Q45047

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKILVIDIQG LIKQVFVRAF SKDNDVEILN AGFNSLNLIN VFLQKFPDLV IIDENTARSN FGNSLNNVLN NISLPVVFIA QNEMLPNFGC LEQSKEKVKL IINKLNFKLT VDLFRSKYLA LIKLELKNLG KNKLISSFEV KRIQAPDFSS NSKVELRENN LNDSSIRKSY RVSDVINFAP KNDPDVIIKY QGLINKHKTG KIIVVGSSTG GTEALRIFLR SFKKDSPPII IVQHMPGGFT KSFAKNLNNE FNIDIKEAED GDILRPGLVI IANGSYHLIV KYSSGNYFVN LLDGPLVSRH KPSVNVLFRS AAMYAGSNAI GVILTGMGDD GAVCMLEMKK NGAYTIAQDQ ETSVVFGMPM EAIKIGAVDK ILPLSEIADH VLRRS
NT Sequence
atgaagatat tagtaattga tattcaaggt cttataaagc aggtttttgt tagagctttt tctaaagata atgatgttga gatattaaat gctggtttta attctttaaa tcttattaat gtatttttac aaaagtttcc agatttagtt attattgatg agaatacagc aagatctaat tttgggaatt ctttaaacaa tgtccttaat aatatatctc ttccggttgt atttattgct caaaatgaaa tgttaccaaa ttttggatgt cttgagcaaa gcaaggaaaa ggttaaatta ataataaata agcttaattt taagcttaca gttgatttat ttcgcagtaa gtatttagct ttaataaagc tggagttgaa aaatctaggc aaaaataaat taatatcttc ttttgaagtt aaaaggattc aagcacccga tttttctagt aattctaaag tagagctgag ggaaaataat ttaaatgatt caagtataag aaaaagttat agagtttctg atgttattaa ttttgctccc aaaaatgatc cggatgttat tattaaatat caaggcctta tcaataagca caaaactggt aaaattattg ttgtaggctc ttcaacgggc ggtacagagg ctttaagaat ttttttaagg tcttttaaaa aagattctcc cccaattatt attgttcagc atatgcctgg aggatttaca aaatcttttg caaaaaacct aaacaatgag tttaatattg atattaaaga agctgaggat ggagacattc ttcgtccagg tcttgtaata attgctaatg gaagttatca tttgattgta aaatatagta gcggaaatta ttttgtaaac ctattagatg gacctcttgt tagtagacat aagccttctg tgaatgtact ttttaggtct gctgcaatgt atgcaggctc taatgctatt ggagttatcc ttacaggtat gggagatgat ggtgctgttt gtatgcttga aatgaaaaaa aatggcgctt atactattgc ccaagatcaa gaaacctctg ttgtttttgg tatgccaatg gaagctataa aaataggggc tgtagacaaa atccttcctt taagcgaaat agcagatcat gttctaagga gatcttaa
Details for BobuA.18906.a.B1.GE38893
HARVESTED ON: 1/29/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 44kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK ILVIDIQGLI KQVFVRAFSK DNDVEILNAG FNSLNLINVF LQKFPDLVII DENTARSNFG NSLNNVLNNI SLPVVFIAQN EMLPNFGCLE QSKEKVKLII NKLNFKLTVD LFRSKYLALI KLELKNLGKN KLISSFEVKR IQAPDFSSNS KVELRENNLN DSSIRKSYRV SDVINFAPKN DPDVIIKYQG LINKHKTGKI IVVGSSTGGT EALRIFLRSF KKDSPPIIIV QHMPGGFTKS FAKNLNNEFN IDIKEAEDGD ILRPGLVIIA NGSYHLIVKY SSGNYFVNLL DGPLVSRHKP SVNVLFRSAA MYAGSNAIGV ILTGTGDDGA VCMLEMKKNG AYTIAQDQET SVVFGMPMEA IKIGAVDKIL PLSEXXXHV
Validated NT Sequence
aaacatgatn nnnnatttcg cttaaaggaa ggattttgtc tacagcccct atttttatag cttccattgg cataccaaaa acaacagagg tttcttgatc ttgggcaata gtataagcgc catttttttt catttcaagc atacaaacag caccatcatc tcccgtacct gtaaggataa ctccaatagc attagagcct gcatacattg cagcagacct aaaaagtaca ttcacagaag gcttatgtct actaacaaga ggtccatcta ataggtttac aaaataattt ccgctactat attttacaat caaatgataa cttccattag caattattac aagacctgga cgaagaatgt ctccatcctc agcttcttta atatcaatat taaactcatt gtttaggttt tttgcaaaag attttgtaaa tcctccaggc atatgctgaa caataataat tgggggagaa tcttttttaa aagaccttaa aaaaattctt aaagcctctg taccgcccgt tgaagagcct acaacaataa ttttaccagt tttgtgctta ttgataaggc cttgatattt aataataaca tccggatcat ttttgggagc aaaattaata acatcagaaa ctctataact ttttcttata cttgaatcat ttaaattatt ttccctcagc tctactttag aattactaga aaaatcgggt gcttgaatcc ttttaacttc aaaagaagat attaatttat ttttgcctag atttttcaac tccagcttta ttaaagctaa atacttactg cgaaataaat caactgtaag cttaaaatta agcttattta ttattaattt aaccttttcc ttgctttgct caagacatcc aaaatttggt aacatttcat tttgagcaat aaatacaacc ggaagagata tattattaag gacattgttt aaagaattcc caaaattaga tcttgctgta ttctcatcaa taataactaa atctggaaac ttttgtaaaa atacattaat aagatttaaa gaattaaaac cagcatttaa tatctcaaca tcattatctt tagaaaaagc tctaacaaaa acctgcttta taagaccttg aatatcaatt actaatatct tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMK ILVIDIQGLI KQVFVRAFSK DNDVEILNAG FNSLNLINVF LQKFPDLVII DENTARSNFG NSLNNVLNNI SLPVVFIAQN EMLPNFGCLE QSKEKVKLII NKLNFKLTVD LFRSKYLALI KLELKNLGKN KLISSFEVKR IQAPDFSSNS KVELRENNLN DSSIRKSYRV SDVINFAPKN DPDVIIKYQG LINKHKTGKI IVVGSSTGGT EALRIFLRSF KKDSPPIIIV QHMPGGFTKS FAKNLNNEFN IDIKEAEDGD ILRPGLVIIA NGSYHLIVKY SSGNYFVNLL DGPLVSRHKP SVNVLFRSAA MYAGSNAIGV ILTGMGDDGA VCMLEMKKNG AYTIAQDQET SVVFGMPMEA IKIGAVDKIL PLSEIADHVL RRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga tattagtaat tgatattcaa ggtcttataa agcaggtttt tgttagagct ttttctaaag ataatgatgt tgagatatta aatgctggtt ttaattcttt aaatcttatt aatgtatttt tacaaaagtt tccagattta gttattattg atgagaatac agcaagatct aattttggga attctttaaa caatgtcctt aataatatat ctcttccggt tgtatttatt gctcaaaatg aaatgttacc aaattttgga tgtcttgagc aaagcaagga aaaggttaaa ttaataataa ataagcttaa ttttaagctt acagttgatt tatttcgcag taagtattta gctttaataa agctggagtt gaaaaatcta ggcaaaaata aattaatatc ttcttttgaa gttaaaagga ttcaagcacc cgatttttct agtaattcta aagtagagct gagggaaaat aatttaaatg attcaagtat aagaaaaagt tatagagttt ctgatgttat taattttgct cccaaaaatg atccggatgt tattattaaa tatcaaggcc ttatcaataa gcacaaaact ggtaaaatta ttgttgtagg ctcttcaacg ggcggtacag aggctttaag aattttttta aggtctttta aaaaagattc tcccccaatt attattgttc agcatatgcc tggaggattt acaaaatctt ttgcaaaaaa cctaaacaat gagtttaata ttgatattaa agaagctgag gatggagaca ttcttcgtcc aggtcttgta ataattgcta atggaagtta tcatttgatt gtaaaatata gtagcggaaa ttattttgta aacctattag atggacctct tgttagtaga cataagcctt ctgtgaatgt actttttagg tctgctgcaa tgtatgcagg ctctaatgct attggagtta tccttacagg tatgggagat gatggtgctg tttgtatgct tgaaatgaaa aaaaatggcg cttatactat tgcccaagat caagaaacct ctgttgtttt tggtatgcca atggaagcta taaaaatagg ggctgtagac aaaatccttc ctttaagcga aatagcagat catgttctaa ggagatcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat