BobuA.18905.b

Uncharacterized protein (BB_K47)

CENTER ID: BobuA.18905.b
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18905.b.B2.GE38803 mature protein 18 328
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1465
RefSeq: NP_045618.1
RefSeq: WP_010890350.1
UniProt: H7C7M2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLCLIKIFI IPNLVFSSLF LFESCSGFLS KKSIEQFALA LKDHQENKNT TNTSVDKNSK EIESPKDVTS SNKKTYDPIL QVGSNQHMSD DPGANNKESL PNSSPAIIQN DSHAQNNVKM EENKSATPQH DPIEQSNFKN SLTTTSKTPA IPSEEEIKAN LDEFAQEEYE QTSLSEIKNA TQIVNHANPE NKLNNTLLEF EKDYETLSNL LFSNLDASPL NRKIKTIMPK LQEMRSFMEQ ATNSWVSAKG MLDEAKDKLA ESIYKRLYNG NSYRFGGSFN GRDMQHAKNL AYRAIDFASA CIEYTQKAID YLQQGNSCKK EIENIFKL
NT Sequence
atgcgattat gtttaataaa aatttttatt atacctaatt tagtatttag ttctcttttt ttatttgaaa gttgttctgg ttttctatct aaaaaatcta tagaacagtt tgcattagca ttaaaagatc atcaagaaaa taaaaatact actaatactt cagtagataa aaatagtaag gaaattgaat ctcctaaaga cgttacatca tcaaataaaa aaacttatga tccaatctta caagtaggtt ctaatcaaca tatgtcagat gatcctggtg ctaataataa agaatcccta ccaaattcaa gtccagcaat aatacaaaat gactcgcatg ctcaaaataa tgtaaagatg gaagaaaata aatcagctac tccacaacat gatccaattg aacaaagtaa ttttaaaaat agccttacta caacaagtaa aactcctgct attccttcag aagaagaaat taaagctaac ttagatgaat ttgcacaaga agagtatgag caaacatctc tttcagaaat taaaaatgcc acgcaaattg ttaatcatgc taatcctgaa aacaaattaa acaatacact ccttgagttt gaaaaagatt atgaaacttt atcaaacttg ttattctcta atttagacgc atctcctttg aatagaaaaa taaagactat tatgcctaaa ttacaagaaa tgcgttcttt tatggagcaa gcaactaatt cttgggtatc tgctaaaggc atgctagatg aggctaagga taaactagca gaatctattt ataaaagact atacaatggc aattcatacc ggttcggtgg cagttttaac ggacgtgata tgcaacatgc aaaaaattta gcatacagag ctatagactt tgcttctgca tgcattgaat atacacaaaa agctattgat tatcttcaac agggaaattc ttgcaaaaaa gaaatagaaa atatattcaa gctttaa
Details for BobuA.18905.b.B2.GE38803
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 36kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LFLFESCSGF LSKKSIEQFA LALKDHQENK NTTNTSVDKN SKEIESPKDV TSSNKKTYDP ILQVGSNQHM SDDPGANNKE SLPNSSPAII QNDSHAQNNV KMEENKSATP QHDPIEQSNF KNSLTTTSKT PAIPSEEEIK ANLDEFAQEE YEQTSLSEIK NATQIVNHAN PENKLNNTLL EFEKDYETLS NLLFSNLDAS PLNRKIKTIM PKLQEMRSFM EQATNSWVSA KGMLDEAKDK LAESIYKRLY NGNSYRFGGS FNGRDMQHAK NLAYRAIDFA SACIEYTQKA IDYLQQGNSC KKEIENIFKL
Validated NT Sequence
tcttactcac ttaaagcttg aatatatttt ctatttcttt tttgcaagaa tttccctgtt gaagataatc aatagctttt tgtgtatatt caatgcatgc agaagcaaag tctatagctc tgtatgctaa attttttgca tgttgcatat cacgtccgtt aaaactgcca ccgaaccggt atgaattgcc attgtatagt cttttataaa tagattctgc tagtttatcc ttagcctcat ctagcatgcc tttagcagat acccaagaat tagttgcttg ctccataaaa gaacgcattt cttgtaattt aggcataata gtctttattt ttctattcaa aggagatgcg tctaaattag agaataacaa gtttgataaa gtttcataat ctttttcaaa ctcaaggagt gtattgttta atttgttttc aggattagca tgattaacaa tttgcgtggc atttttaatt tctgaaagag atgtttgctc atactcttct tgtgcaaatt catctaagtt agctttaatt tcttcttctg aaggaatagc aggagtttta cttgttgtag taaggctatt tttaaaatta ctttgttcaa ttggatcatg ttgtggagta gctgatttat tttcttccat ctttacatta ttttgagcat gcgagtcatt ttgtattatt gctggacttg aatttggtag ggattcttta ttattagcac caggatcatc tgacatatgt tgattagaac ctacttgtaa gattggatca taagtttttt tatttgatga tgtaacgtct ttaggagatt caatttcctt actattttta tctactgaag tattagtagt atttttattt tcttgatgat cttttaatgc taatgcaaac tgttctatag attttttaga tagaaaacca gaacaacttt caaataaaaa aagagacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHSL FLFESCSGFL SKKSIEQFAL ALKDHQENKN TTNTSVDKNS KEIESPKDVT SSNKKTYDPI LQVGSNQHMS DDPGANNKES LPNSSPAIIQ NDSHAQNNVK MEENKSATPQ HDPIEQSNFK NSLTTTSKTP AIPSEEEIKA NLDEFAQEEY EQTSLSEIKN ATQIVNHANP ENKLNNTLLE FEKDYETLSN LLFSNLDASP LNRKIKTIMP KLQEMRSFME QATNSWVSAK GMLDEAKDKL AESIYKRLYN GNSYRFGGSF NGRDMQHAKN LAYRAIDFAS ACIEYTQKAI DYLQQGNSCK KEIENIFKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctcttt ttttatttga aagttgttct ggttttctat ctaaaaaatc tatagaacag tttgcattag cattaaaaga tcatcaagaa aataaaaata ctactaatac ttcagtagat aaaaatagta aggaaattga atctcctaaa gacgttacat catcaaataa aaaaacttat gatccaatct tacaagtagg ttctaatcaa catatgtcag atgatcctgg tgctaataat aaagaatccc taccaaattc aagtccagca ataatacaaa atgactcgca tgctcaaaat aatgtaaaga tggaagaaaa taaatcagct actccacaac atgatccaat tgaacaaagt aattttaaaa atagccttac tacaacaagt aaaactcctg ctattccttc agaagaagaa attaaagcta acttagatga atttgcacaa gaagagtatg agcaaacatc tctttcagaa attaaaaatg ccacgcaaat tgttaatcat gctaatcctg aaaacaaatt aaacaataca ctccttgagt ttgaaaaaga ttatgaaact ttatcaaact tgttattctc taatttagac gcatctcctt tgaatagaaa aataaagact attatgccta aattacaaga aatgcgttct tttatggagc aagcaactaa ttcttgggta tctgctaaag gcatgctaga tgaggctaag gataaactag cagaatctat ttataaaaga ctatacaatg gcaattcata ccggttcggt ggcagtttta acggacgtga tatgcaacat gcaaaaaatt tagcatacag agctatagac tttgcttctg catgcattga atatacacaa aaagctattg attatcttca acagggaaat tcttgcaaaa aagaaataga aaatatattc aagctttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t