BobuA.18901.c

Methyl-accepting chemotaxis protein (BB_0578)

CENTER ID: BobuA.18901.c
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18901.c.B1.GE38790 full length 1 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18901.c.B1.PS38077 full length 1 389

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.969
RefSeq: NP_212712.1
UniProt: O51525

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKFSFFKKK KKAVDLNLLN VVGNDKKDLS TYEYKAPVKE MLKGFYHTKA SISTLKVGFE SLQRILFSEI EDFDQIFSTF VEMTNNARNQ ISVIFSDLGK NNKEKLNQIS SVIVGIQGSL ETISNFLGAT NMISLNAKLE AARAKEYGKG FSVVADEIKR LSDQAKGVMN MISVKEIEEV SKDLISHNLK DLQIDIDKFF AEILEQLNYL ESIFKRFSRS QEEFSSLIES LESIDANMAY YSRNCDSLIG SDTFMLSNDE FLKELEFIIS DQFSWINNLR LLVEGQRTIF IQTDASKHGF GLFYKGLSPK NDAIRQLWEE VYIPYLNINK FAAEILIIFR VENRSDSGLR QAKDFLSQAE SLSEEIVRKL EHIKKMVIEL DNQGISIFS
NT Sequence
atgaaaaaat tttcgttttt taaaaaaaag aaaaaagctg ttgatttaaa tcttttaaat gttgttggta atgacaaaaa agatttgagt acttacgaat ataaggctcc tgttaaggaa atgctaaagg gattttatca taccaaagct tccatttcta ctttaaaagt aggatttgag tcattacaaa ggattttgtt ttctgaaata gaagattttg atcagatatt ttctactttt gttgagatga caaataatgc tcgtaatcag ataagcgtta tttttagtga tcttggtaaa aataataagg aaaaattaaa tcaaatatct tctgttattg ttggaattca aggcagctta gaaacaataa gtaatttttt aggtgctacc aatatgattt ctcttaatgc aaagcttgag gctgcaagag ctaaagaata tggtaaagga ttttctgttg ttgcagatga gatcaagcga ctttctgatc aggcaaaagg tgttatgaat atgatttctg taaaggaaat tgaggaggtt tctaaagatt tgatttctca taaccttaag gatttgcaga ttgatattga taagttcttt gccgagattc ttgaacagct taattatctt gagagcatat ttaagcgctt ttcaagaagt caagaggaat tttcttcttt gattgaaagt cttgagagta tagatgctaa tatggcttat tattcaagaa actgtgattc tttgattggc tctgatactt ttatgctgtc taatgatgaa tttttaaaag agctagaatt tattatctca gaccaatttt cttggattaa taatttgaga ctacttgttg aggggcaaag gacaatattc attcagacag atgcttccaa gcatgggttt ggattgtttt ataaaggatt gtctcccaag aatgatgcta tcaggcagtt gtgggaagaa gtatacattc cttatttaaa tattaataag tttgcagctg aaattttgat tatatttagg gtagagaatc gtagtgatag cggtttaagg caagccaaag attttttgtc tcaagctgag agtttatctg aagaaattgt tagaaaactt gagcacatta aaaagatggt aattgaattg gataatcaag gaattagtat tttttcttga
Details for BobuA.18901.c.B1.GE38790
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 46kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK KFSFFKKKKK AVDLNLLNVV GNDKKDLSTY EYKAPVKEML KGFYHTKASI STLKVGFESL QRILFSEIED FDQIFSTFVE MTNNARNQIS VIFSDLGKNN KEKLNQISSV IVGIQGSLET ISNFLGATNM ISLNAKLEAA RAKEYGKGFS VVADEIKRLS DQAKGVMNMI SVKEIEEVSK DLISHNLKDL QIDIDKFFAE ILEQLNYLES IFKRFSRSQE EFSSLIESLE SIDANMAYYS RNCDSLIGSD TFMLSNDEFL KELEFIISDQ FSWINNLRLL VEGQRTIFIQ TDASKHGFGL FYKGLSPKND AIRQLWEEVY IPYLNINKFA AEILIIFRVE NRSDSGLRQA KDFLSQAESL SEEIVRKLEH IKKMVIELD
Validated NT Sequence
attatccaat tcaattacca tctttttaat gtgctcaagt tttctaacaa tttcttcaga taaactctca gcttgagaca aaaaatcttt ggcttgcctt aaaccgctat cactacgatt ctctacccta aatataatca aaatttcagc tgcaaactta ttaatattta aataaggaat gtatacttct tcccacaact gcctgatagc atcattcttg ggagacaatc ctttataaaa caatccaaac ccatgcttgg aagcatctgt ctgaatgaat attgtccttt gcccctcaac aagtagtctc aaattattaa tccaagaaaa ttggtctgag ataataaatt ctagctcttt taaaaattca tcattagaca gcataaaagt atcagagcca atcaaagaat cacagtttct tgaataataa gccatattag catctatact ctcaagactt tcaatcaaag aagaaaattc ctcttgactt cttgaaaagc gcttaaatat gctctcaaga taattaagct gttcaagaat ctcggcaaag aacttatcaa tatcaatctg caaatcctta aggttatgag aaatcaaatc tttagaaacc tcctcaattt cctttacaga aatcatattc ataacacctt ttgcctgatc agaaagtcgc ttgatctcat ctgcaacaac agaaaatcct ttaccatatt ctttagctct tgcagcctca agctttgcat taagagaaat catattggta gcacctaaaa aattacttat tgtttctaag ctgccttgaa ttccaacaat aacagaagat atttgattta atttttcctt attattttta ccaagatcac taaaaataac gcttatctga ttacgagcat tatttgtcat ctcaacaaaa gtagaaaata tctgatcaaa atcttctatt tcagaaaaca aaatcctttg taatgactca aatcctactt ttaaagtaga aatggaagct ttggtatgat aaaatccctt tagcatttcc ttaacaggag ccttatattc gtaagtactc aaatcttttt tgtcattacc aacaacattt aaaagattta aatcaacagc ttttttcttt tttttaaaaa acgaaaattt tttcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMK KFSFFKKKKK AVDLNLLNVV GNDKKDLSTY EYKAPVKEML KGFYHTKASI STLKVGFESL QRILFSEIED FDQIFSTFVE MTNNARNQIS VIFSDLGKNN KEKLNQISSV IVGIQGSLET ISNFLGATNM ISLNAKLEAA RAKEYGKGFS VVADEIKRLS DQAKGVMNMI SVKEIEEVSK DLISHNLKDL QIDIDKFFAE ILEQLNYLES IFKRFSRSQE EFSSLIESLE SIDANMAYYS RNCDSLIGSD TFMLSNDEFL KELEFIISDQ FSWINNLRLL VEGQRTIFIQ TDASKHGFGL FYKGLSPKND AIRQLWEEVY IPYLNINKFA AEILIIFRVE NRSDSGLRQA KDFLSQAESL SEEIVRKLEH IKKMVIELDN QGISIFS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aattttcgtt ttttaaaaaa aagaaaaaag ctgttgattt aaatctttta aatgttgttg gtaatgacaa aaaagatttg agtacttacg aatataaggc tcctgttaag gaaatgctaa agggatttta tcataccaaa gcttccattt ctactttaaa agtaggattt gagtcattac aaaggatttt gttttctgaa atagaagatt ttgatcagat attttctact tttgttgaga tgacaaataa tgctcgtaat cagataagcg ttatttttag tgatcttggt aaaaataata aggaaaaatt aaatcaaata tcttctgtta ttgttggaat tcaaggcagc ttagaaacaa taagtaattt tttaggtgct accaatatga tttctcttaa tgcaaagctt gaggctgcaa gagctaaaga atatggtaaa ggattttctg ttgttgcaga tgagatcaag cgactttctg atcaggcaaa aggtgttatg aatatgattt ctgtaaagga aattgaggag gtttctaaag atttgatttc tcataacctt aaggatttgc agattgatat tgataagttc tttgccgaga ttcttgaaca gcttaattat cttgagagca tatttaagcg cttttcaaga agtcaagagg aattttcttc tttgattgaa agtcttgaga gtatagatgc taatatggct tattattcaa gaaactgtga ttctttgatt ggctctgata cttttatgct gtctaatgat gaatttttaa aagagctaga atttattatc tcagaccaat tttcttggat taataatttg agactacttg ttgaggggca aaggacaata ttcattcaga cagatgcttc caagcatggg tttggattgt tttataaagg attgtctccc aagaatgatg ctatcaggca gttgtgggaa gaagtataca ttccttattt aaatattaat aagtttgcag ctgaaatttt gattatattt agggtagaga atcgtagtga tagcggttta aggcaagcca aagatttttt gtctcaagct gagagtttat ctgaagaaat tgttagaaaa cttgagcaca ttaaaaagat ggtaattgaa ttggataatc aaggaattag tattttttct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BobuA.18901.c.B1.PS38077
PURIFICATION DATe: 10/12/2016
CONCENTRATION: 8.17mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK KFSFFKKKKK AVDLNLLNVV GNDKKDLSTY EYKAPVKEML KGFYHTKASI STLKVGFESL QRILFSEIED FDQIFSTFVE MTNNARNQIS VIFSDLGKNN KEKLNQISSV IVGIQGSLET ISNFLGATNM ISLNAKLEAA RAKEYGKGFS VVADEIKRLS DQAKGVMNMI SVKEIEEVSK DLISHNLKDL QIDIDKFFAE ILEQLNYLES IFKRFSRSQE EFSSLIESLE SIDANMAYYS RNCDSLIGSD TFMLSNDEFL KELEFIISDQ FSWINNLRLL VEGQRTIFIQ TDASKHGFGL FYKGLSPKND AIRQLWEEVY IPYLNINKFA AEILIIFRVE NRSDSGLRQA KDFLSQAESL SEEIVRKLEH IKKMVIELD
Validated NT Sequence
attatccaat tcaattacca tctttttaat gtgctcaagt tttctaacaa tttcttcaga taaactctca gcttgagaca aaaaatcttt ggcttgcctt aaaccgctat cactacgatt ctctacccta aatataatca aaatttcagc tgcaaactta ttaatattta aataaggaat gtatacttct tcccacaact gcctgatagc atcattcttg ggagacaatc ctttataaaa caatccaaac ccatgcttgg aagcatctgt ctgaatgaat attgtccttt gcccctcaac aagtagtctc aaattattaa tccaagaaaa ttggtctgag ataataaatt ctagctcttt taaaaattca tcattagaca gcataaaagt atcagagcca atcaaagaat cacagtttct tgaataataa gccatattag catctatact ctcaagactt tcaatcaaag aagaaaattc ctcttgactt cttgaaaagc gcttaaatat gctctcaaga taattaagct gttcaagaat ctcggcaaag aacttatcaa tatcaatctg caaatcctta aggttatgag aaatcaaatc tttagaaacc tcctcaattt cctttacaga aatcatattc ataacacctt ttgcctgatc agaaagtcgc ttgatctcat ctgcaacaac agaaaatcct ttaccatatt ctttagctct tgcagcctca agctttgcat taagagaaat catattggta gcacctaaaa aattacttat tgtttctaag ctgccttgaa ttccaacaat aacagaagat atttgattta atttttcctt attattttta ccaagatcac taaaaataac gcttatctga ttacgagcat tatttgtcat ctcaacaaaa gtagaaaata tctgatcaaa atcttctatt tcagaaaaca aaatcctttg taatgactca aatcctactt ttaaagtaga aatggaagct ttggtatgat aaaatccctt tagcatttcc ttaacaggag ccttatattc gtaagtactc aaatcttttt tgtcattacc aacaacattt aaaagattta aatcaacagc ttttttcttt tttttaaaaa acgaaaattt tttcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMK KFSFFKKKKK AVDLNLLNVV GNDKKDLSTY EYKAPVKEML KGFYHTKASI STLKVGFESL QRILFSEIED FDQIFSTFVE MTNNARNQIS VIFSDLGKNN KEKLNQISSV IVGIQGSLET ISNFLGATNM ISLNAKLEAA RAKEYGKGFS VVADEIKRLS DQAKGVMNMI SVKEIEEVSK DLISHNLKDL QIDIDKFFAE ILEQLNYLES IFKRFSRSQE EFSSLIESLE SIDANMAYYS RNCDSLIGSD TFMLSNDEFL KELEFIISDQ FSWINNLRLL VEGQRTIFIQ TDASKHGFGL FYKGLSPKND AIRQLWEEVY IPYLNINKFA AEILIIFRVE NRSDSGLRQA KDFLSQAESL SEEIVRKLEH IKKMVIELDN QGISIFS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA AATTTTCGTT TTTTAAAAAA AAGAAAAAAG CTGTTGATTT AAATCTTTTA AATGTTGTTG GTAATGACAA AAAAGATTTG AGTACTTACG AATATAAGGC TCCTGTTAAG GAAATGCTAA AGGGATTTTA TCATACCAAA GCTTCCATTT CTACTTTAAA AGTAGGATTT GAGTCATTAC AAAGGATTTT GTTTTCTGAA ATAGAAGATT TTGATCAGAT ATTTTCTACT TTTGTTGAGA TGACAAATAA TGCTCGTAAT CAGATAAGCG TTATTTTTAG TGATCTTGGT AAAAATAATA AGGAAAAATT AAATCAAATA TCTTCTGTTA TTGTTGGAAT TCAAGGCAGC TTAGAAACAA TAAGTAATTT TTTAGGTGCT ACCAATATGA TTTCTCTTAA TGCAAAGCTT GAGGCTGCAA GAGCTAAAGA ATATGGTAAA GGATTTTCTG TTGTTGCAGA TGAGATCAAG CGACTTTCTG ATCAGGCAAA AGGTGTTATG AATATGATTT CTGTAAAGGA AATTGAGGAG GTTTCTAAAG ATTTGATTTC TCATAACCTT AAGGATTTGC AGATTGATAT TGATAAGTTC TTTGCCGAGA TTCTTGAACA GCTTAATTAT CTTGAGAGCA TATTTAAGCG CTTTTCAAGA AGTCAAGAGG AATTTTCTTC TTTGATTGAA AGTCTTGAGA GTATAGATGC TAATATGGCT TATTATTCAA GAAACTGTGA TTCTTTGATT GGCTCTGATA CTTTTATGCT GTCTAATGAT GAATTTTTAA AAGAGCTAGA ATTTATTATC TCAGACCAAT TTTCTTGGAT TAATAATTTG AGACTACTTG TTGAGGGGCA AAGGACAATA TTCATTCAGA CAGATGCTTC CAAGCATGGG TTTGGATTGT TTTATAAAGG ATTGTCTCCC AAGAATGATG CTATCAGGCA GTTGTGGGAA GAAGTATACA TTCCTTATTT AAATATTAAT AAGTTTGCAG CTGAAATTTT GATTATATTT AGGGTAGAGA ATCGTAGTGA TAGCGGTTTA AGGCAAGCCA AAGATTTTTT GTCTCAAGCT GAGAGTTTAT CTGAAGAAAT TGTTAGAAAA CTTGAGCACA TTAAAAAGAT GGTAATTGAA TTGGATAATC AAGGAATTAG TATTTTTTCT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT