BobuA.18901.b

Methyl-accepting chemotaxis protein (BB_0681)

CENTER ID: BobuA.18901.b
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18901.b.B2.GE38788 soluble domain N-term 31 319
BobuA.18901.b.B3.GE38789 MCP signalling (HAMP) domain 341 633
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1072
RefSeq: NP_212815.2
UniProt: O51624

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLKARMLLL VLILIAFFIS ILFFAFGMLI NSKLVDQQFN LMINLIESIK SSFNLYISSM EEKVRVSSMY FNSAEKFNEA SKIKSKRLSF ISDQSEILIQ TGSNMMVTDK EGKIVFTTAV KDNSDFGKSI GDREYFTKLK ESNSIVYNSF VMLADPGSIE ESLLKDISKI KNKKGQIPYI LIGMPLRDFE TDNIFGYFMF LYSMDYIYRS FRGINFGILS SGRALAYDTT GRLLVHHVVL PGDILTDISA SYSNIIKKTS EDLLQKNKEI STVYYYDPKS NKKYVGISQK VLLNLSNNKF ILLMRTSEDD FYYMSRATTI ILAISFVFTL LMLAIATLYL VKKLSSSLNK ILEYSERLAS GNFTADINFG KWDTVELYSL YEGLEQLRTN FSSVAKGVIE NLDYLYENAI QIANASQNLS SGAVEQASTL EQMTANIEQI SQGVSENTEN AATTEKIAVN TNERTKEGHK SVVKAIEAMT VITEKIGIID EITRQTNLLA LNASIEAARV GEKGKGFEVV AAEVRKLADQ SKESAREIID IANRSLTVAS RAGENFEQIV PGMEQTARLV KNISNESYKQ SVQIEQFKNA IEQVSQLVQT TASSSEELSA MSEKMLESVK DLKESVDYFK IEK
NT Sequence
atgaagctta aagctaggat gttgctactt gttcttattc tgatagcatt ctttatatca attttgtttt ttgcttttgg aatgcttatt aatagtaaat tggtggatca acagtttaat cttatgataa atcttattga aagcattaaa agttctttta atctttacat ctcttcaatg gaagagaaag ttagggttag ttccatgtat ttcaactctg ctgaaaaatt taatgaggct agtaaaatta aatccaaaag gttgagcttt atttcagatc aatctgaaat tcttattcaa accggtagta atatgatggt tacagacaaa gaaggtaaaa tagtgtttac tacggcggtt aaggataata gtgattttgg caaatctatt ggggatagag aatattttac aaaacttaag gagtctaata gtattgttta caattccttt gtcatgttgg cagatcccgg gtctattgag gagtctttac ttaaagatat ttccaagata aaaaataaaa aaggtcagat tccttacata ttaataggta tgccattaag agattttgaa acagataaca tttttggtta ttttatgttt ctttattcaa tggattatat atataggtct tttagaggga ttaattttgg aatactctct agcggtcgtg cgctagctta tgatactacg ggtagattgt tggttcatca tgtagtattg ccaggtgata ttttgactga tattagtgct tcttattcca atattattaa gaaaacatct gaagatttgt tgcaaaagaa taaagaaatt tcaactgttt attattatga tcctaaaagc aataagaaat atgtgggaat tagtcaaaag gtgttattaa acttgtctaa taataaattt attcttttaa tgagaacttc agaggacgat ttttattaca tgtcacgagc tacaactata atcttagcaa ttagttttgt atttacatta cttatgcttg ctattgcaac tctttatctt gtgaaaaagt taagctcttc tttgaataag atactggaat attctgagag acttgcttct ggtaatttta ctgctgatat taattttggc aaatgggata ctgtagagct ttacagtttg tacgaagggc ttgagcagtt gagaaccaat ttttcttcag ttgcaaaagg agttattgaa aatctagatt atctttatga aaatgcaatt caaatagcaa atgcaagcca gaatttaagt tctggcgctg ttgagcaggc ttctacttta gagcaaatga cagcaaatat tgagcaaatt tcacaaggtg tttctgagaa tactgaaaat gcagctacta ctgaaaaaat tgctgttaat actaatgaaa ggactaaaga ggggcataaa tctgttgtta aggctattga ggcaatgact gtaattactg aaaaaattgg aattattgat gagataacaa ggcaaaccaa tttgcttgct ttaaatgcct cgattgaagc tgcacgagtg ggagaaaagg gcaagggatt tgaagtggta gctgctgagg ttagaaagct tgcagatcaa agcaaagaat cagcaagaga gattattgat attgcaaaca gaagtttaac tgttgcaagt cgtgctgggg aaaattttga acaaatagtt cctggtatgg aacaaacagc cagacttgta aaaaatattt ctaatgaaag ttataagcaa agtgttcaaa tagagcaatt taaaaatgca atagagcagg ttagtcagtt agtccaaact acagcctcaa gcagtgaaga gctttctgca atgtctgaaa agatgttaga gagtgtaaaa gatttaaaag aatctgttga ttattttaag atcgaaaagt aa
Details for BobuA.18901.b.B2.GE38788
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 34kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN SKLVDQQFNL MINLIESIKS SFNLYISSME EKVRVSSMYF NSAEKFNEAS KIKSKRLSFI SDQSEILIQT GSNMMVTDKE GKIVFTTAVK DNSDFGKSIG DREYFTKLKE SNSIVYNSFV MLADPGSIEE SLLKDISKIK NKKGQIPYIL IGMPLRDFET DNIFGYFMFL YSMDYIYRSF RGINFGILSS GRALAYDTTG RLLVHHVVLP GDILTDISAS YSNIIKKTSE DLLQKNKEIS TVYYYDPKSN KKYVGISQKV LLNLSNNKFI LLMRTSEDDF XXXSRATT
Validated NT Sequence
tcacttaagt tgtagctcgt ganannnann aaaaatcgtc ctctgaagtt ctcattaaaa gaataaattt attattagac aagtttaata acaccttttg actaattccc acatatttct tattgctttt aggatcataa taataaacag ttgaaatttc tttattcttt tgcaacaaat cttcagatgt tttcttaata atattggaat aagaagcact aatatcagtc aaaatatcac ctggcaatac tacatgatga accaacaatc tacccgtagt atcataagct agcgcacgac cgctagagag tattccaaaa ttaatccctc taaaagacct atatatataa tccattgaat aaagaaacat aaaataacca aaaatgttat ctgtttcaaa atctcttaat ggcataccta ttaatatgta aggaatctga ccttttttat tttttatctt ggaaatatct ttaagtaaag actcctcaat agacccggga tctgccaaca tgacaaagga attgtaaaca atactattag actccttaag ttttgtaaaa tattctctat ccccaataga tttgccaaaa tcactattat ccttaaccgc cgtagtaaac actattttac cttctttgtc tgtaaccatc atattactac cggtttgaat aagaatttca gattgatctg aaataaagct caaccttttg gatttaattt tactagcctc attaaatttt tcagcagagt tgaaatacat ggaactaacc ctaactttct cttccattga agagatgtaa agattaaaag aacttttaat gctttcaata agatttatca taagattaaa ctgttgatcc accaatttac tattcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHNS KLVDQQFNLM INLIESIKSS FNLYISSMEE KVRVSSMYFN SAEKFNEASK IKSKRLSFIS DQSEILIQTG SNMMVTDKEG KIVFTTAVKD NSDFGKSIGD REYFTKLKES NSIVYNSFVM LADPGSIEES LLKDISKIKN KKGQIPYILI GMPLRDFETD NIFGYFMFLY SMDYIYRSFR GINFGILSSG RALAYDTTGR LLVHHVVLPG DILTDISASY SNIIKKTSED LLQKNKEIST VYYYDPKSNK KYVGISQKVL LNLSNNKFIL LMRTSEDDFY YMSRATT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatagta aattggtgga tcaacagttt aatcttatga taaatcttat tgaaagcatt aaaagttctt ttaatcttta catctcttca atggaagaga aagttagggt tagttccatg tatttcaact ctgctgaaaa atttaatgag gctagtaaaa ttaaatccaa aaggttgagc tttatttcag atcaatctga aattcttatt caaaccggta gtaatatgat ggttacagac aaagaaggta aaatagtgtt tactacggcg gttaaggata atagtgattt tggcaaatct attggggata gagaatattt tacaaaactt aaggagtcta atagtattgt ttacaattcc tttgtcatgt tggcagatcc cgggtctatt gaggagtctt tacttaaaga tatttccaag ataaaaaata aaaaaggtca gattccttac atattaatag gtatgccatt aagagatttt gaaacagata acatttttgg ttattttatg tttctttatt caatggatta tatatatagg tcttttagag ggattaattt tggaatactc tctagcggtc gtgcgctagc ttatgatact acgggtagat tgttggttca tcatgtagta ttgccaggtg atattttgac tgatattagt gcttcttatt ccaatattat taagaaaaca tctgaagatt tgttgcaaaa gaataaagaa atttcaactg tttattatta tgatcctaaa agcaataaga aatatgtggg aattagtcaa aaggtgttat taaacttgtc taataataaa tttattcttt taatgagaac ttcagaggac gatttttatt acatgtcacg agctacaact tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BobuA.18901.b.B3.GE38789
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 33kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV KKLSSSLNKI LEYSERLASG NFTADINFGK WDTVELYSLY EGLEQLRTNF SSVAKGVIEN LDYLYENAIQ IANASQNLSS GAVEQASTLE QMTANIEQIS QGVSENTENA ATTEKIAVNT NERTKEGHKS VVKAIEAMTV ITEKIGIIDE ITRQTNLLAL NASIEAARVG EKGKGFEVVA AEVRKLADQS KESAREIIDI ANRSLTVASR AGENFEQIVP GMEQTARLVK NISNESYKQS VQIEQFKNAI EQVSQLVQTT ASSSEELSAM SEKMLESVKD LKESXXXFKI EK
Validated NT Sequence
cacttacttt tcgatcttaa aannnnnnan agattctttt aaatctttta cactttctaa catcttttca gacattgcag aaagctcttc actgcttgag gctgtagttt ggactaactg actaacctgc tctattgcat ttttaaattg ctctatttga acactttgct tataactttc attagaaata ttttttacaa gtctggctgt ttgttccata ccaggaacta tttgttcaaa attttcccca gcacgacttg caacagttaa acttctgttt gcaatatcaa taatctctct tgctgattct ttgctttgat ctgcaagctt tctaacctca gcagctacca cttcaaatcc cttgcccttt tctcccactc gtgcagcttc aatcgaggca tttaaagcaa gcaaattggt ttgccttgtt atctcatcaa taattccaat tttttcagta attacagtca ttgcctcaat agccttaaca acagatttat gcccctcttt agtcctttca ttagtattaa cagcaatttt ttcagtagta gctgcatttt cagtattctc agaaacacct tgtgaaattt gctcaatatt tgctgtcatt tgctctaaag tagaagcctg ctcaacagcg ccagaactta aattctggct tgcatttgct atttgaattg cattttcata aagataatct agattttcaa taactccttt tgcaactgaa gaaaaattgg ttctcaactg ctcaagccct tcgtacaaac tgtaaagctc tacagtatcc catttgccaa aattaatatc agcagtaaaa ttaccagaag caagtctctc agaatattcc agtatcttat tcaaagaaga gcttaacttt ttcaccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHVK KLSSSLNKIL EYSERLASGN FTADINFGKW DTVELYSLYE GLEQLRTNFS SVAKGVIENL DYLYENAIQI ANASQNLSSG AVEQASTLEQ MTANIEQISQ GVSENTENAA TTEKIAVNTN ERTKEGHKSV VKAIEAMTVI TEKIGIIDEI TRQTNLLALN ASIEAARVGE KGKGFEVVAA EVRKLADQSK ESAREIIDIA NRSLTVASRA GENFEQIVPG MEQTARLVKN ISNESYKQSV QIEQFKNAIE QVSQLVQTTA SSSEELSAMS EKMLESVKDL KESVDYFKIE K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaaa agttaagctc ttctttgaat aagatactgg aatattctga gagacttgct tctggtaatt ttactgctga tattaatttt ggcaaatggg atactgtaga gctttacagt ttgtacgaag ggcttgagca gttgagaacc aatttttctt cagttgcaaa aggagttatt gaaaatctag attatcttta tgaaaatgca attcaaatag caaatgcaag ccagaattta agttctggcg ctgttgagca ggcttctact ttagagcaaa tgacagcaaa tattgagcaa atttcacaag gtgtttctga gaatactgaa aatgcagcta ctactgaaaa aattgctgtt aatactaatg aaaggactaa agaggggcat aaatctgttg ttaaggctat tgaggcaatg actgtaatta ctgaaaaaat tggaattatt gatgagataa caaggcaaac caatttgctt gctttaaatg cctcgattga agctgcacga gtgggagaaa agggcaaggg atttgaagtg gtagctgctg aggttagaaa gcttgcagat caaagcaaag aatcagcaag agagattatt gatattgcaa acagaagttt aactgttgca agtcgtgctg gggaaaattt tgaacaaata gttcctggta tggaacaaac agccagactt gtaaaaaata tttctaatga aagttataag caaagtgttc aaatagagca atttaaaaat gcaatagagc aggttagtca gttagtccaa actacagcct caagcagtga agagctttct gcaatgtctg aaaagatgtt agagagtgta aaagatttaa aagaatctgt tgattatttt aagatcgaaa agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat