SSGCID: Target Page

BobuA.18901.a

Methyl-accepting chemotaxis protein (BB_0680)

CENTER ID:	BobuA.18901.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18901.a.B2.GE38786	soluble domain N-term		34	353

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.1071
RefSeq:	NP_212814.1
RefSeq:	WP_010889797.1
UniProt:	O51623

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLLKLKYRFV GFLLLFLIFI LLLFSTIFNF VLCGYLEDYY KQLTRAQVRR AAFSLQSFLD TLHVIINGAA SNLALETISE FAMSENRGKD FSESELIDLR KNPKFVIDSV KVSKKYRQYL YNFMANLKND TLFEEFAFFD FEGRVIVSTR HENNMDFGHS EANTNYFKKA VEDYRQNQLK FIGWYSNLSE GISAEVAIRS KQSEKKAFAI IVPVYSPEDK LVCGYLAGYL LNDIVADSFD RFRFGFYKRG NFIYVDPNNI AVNPFEEYNE TSRVSSKFLN VLKDVFSKPP FPSNIASEVS VYTIDRILLS EMGEDCYYAM LPISSKLGEK SGVLIARLPY KDIYGVISSL RFQYILYSVL GIIALSIVLS IRIDRIISFR LNAIRVLVQD MVKGNLDKDY ALDDDENTLD ELGMLSLQVV KMKKAISVAI SSVLRNISYV NKASLEVASS SQNLSSSALQ QASALEEMSA NVEQIASGVN MSANNSYETE QIALKTNENS QIGGRAVEES VIAMQDIVEK VSVIEEIARK TNLLALNAAI EAARAGDEGK GFAVVASEIR KLADLSKISA LEIGELVEDN SKVATEAGVI FKEMLPEIEE TANLVKKISE GSSKQSDQIA QFKMALDQVG EVVQSSASSS EQLSSMSDKM LEKSKELRKS VLFFKIKDSK IENPENDDYD FRLIDCPENS FKDENQNLKS NGISTSNASG HNNYSLDIES ESSVRTINKR VDPKKAIDIA DKDLNFDDDF SEF

NT Sequence

atgttattga agcttaaata caggtttgtt ggatttttat tattgttttt aatttttata ctgctacttt tttccacgat ttttaatttt gttttatgcg gttatttaga agattattat aagcagctta caagggcgca agtaagaaga gcagcttttt ctttgcaatc ttttttagac accctgcatg tcataatcaa tggtgcagct tctaatttgg cacttgaaac catatcagaa tttgcaatgt ctgagaatag aggaaaagat ttctctgagt cggaattgat agatttaaga aaaaatccaa aatttgttat tgactctgta aaggtgagca aaaaatatcg acaatactta tacaatttta tggccaatct taaaaatgat accctttttg aagaattcgc tttttttgat tttgaaggga gagtaattgt tagcacaaga catgagaata atatggattt tggtcattct gaggctaata ccaattattt taaaaaagct gttgaggatt ataggcaaaa ccaattaaaa tttataggtt ggtattcaaa tctttctgaa ggaatatccg cagaagttgc tattaggtct aaacaaagcg aaaaaaaggc ttttgcaata attgtacctg tatattcccc agaagataaa cttgtttgtg ggtatttggc cggatatttg cttaatgata ttgtggcaga tagttttgat agatttagat tcggttttta taaaagaggc aattttattt atgtggatcc caacaatata gcagttaatc cttttgaaga atataatgaa accagcaggg ttagttctaa atttttgaat gttcttaaag atgttttctc taagccccct tttccatcaa acattgccag tgaagtgtcg gtttacacta ttgatagaat acttttgtcc gaaatgggag aagattgtta ttatgcaatg ttgcccataa gtagtaaatt gggagaaaag agtggagtac ttattgctag gcttccttat aaggatattt acggagtaat atctagtcta agatttcagt atattttata ttcagtctta ggcattatag cattaagtat tgttctttca attagaatag acaggattat tagttttcgt ttaaacgcaa ttagagttct agttcaagat atggttaagg gcaatttaga taaagattat gctcttgatg atgatgaaaa tactcttgat gaacttggca tgttaagtct tcaggttgtt aaaatgaaaa aagctatttc tgtagcaatt tctagtgttt tgagaaatat tagctatgta aataaggcaa gtttagaagt tgccagttca agtcaaaatt taagctctag tgcattgcaa caggcatctg ctcttgaaga aatgtcagct aatgttgagc aaatagcctc aggtgtcaac atgagcgcca ataattctta tgaaacagaa caaatagctt taaagacgaa tgaaaattct cagataggtg gtagggccgt tgaagaatct gttattgcta tgcaagacat tgtggagaaa gttagtgtta ttgaagagat agctagaaaa accaatttac ttgctttgaa tgcggctatt gaagctgcaa gagcaggaga tgagggaaag ggatttgctg ttgtggccag tgagattaga aagttggctg atttgagtaa aatttctgct cttgagattg gagagttagt tgaagataac tctaaggtag caactgaagc gggagtgatc tttaaagaaa tgctacccga aattgaagaa acggctaatc ttgttaagaa gatttcagaa ggtagctcta agcaaagcga tcagattgct caatttaaaa tggctttaga tcaggttgga gaagttgttc aatcttcagc ttcaagcagt gagcagcttt ctagtatgtc cgataaaatg ttagaaaagt ctaaggaact tagaaaatct gtattatttt tcaaaattaa agattctaaa attgaaaatc cagaaaatga tgattatgat ttcaggttaa tagattgtcc tgaaaattct tttaaagatg aaaatcaaaa tttgaaaagc aatggaattt ctacttcaaa tgccagtggg cataataatt attctttaga tattgagagc gaatcttctg taagaactat taataagcga gttgatccta aaaaagctat cgatattgct gataaggatt taaattttga tgatgatttt tcagagtttt ag

Details for BobuA.18901.a.B2.GE38786

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG YLEDYYKQLT RAQVRRAAFS LQSFLDTLHV IINGAASNLA LETISEFAMS ENRGKDFSES ELIDLRKNPK FVIDSVKVSK KYRQYLYNFM ANLKNDTLFE EFAFFDFEGR VIVSTRHENN MDFGHSEANT NYFKKAVEDY RQNQLKFIGW YSNLSEGISA EVAIRSKQSE KKAFAIIVPA YSPEDKLVCG YLAGYLLNDI VADSFDRFRF GFYKRGNFIY VDPNNIAVNP FEEYNETSRV SSKFLNVLKD VFSKPPFPSN IASEVSVYTI DRILLSEMGE DCYYAMLPIS SKLGEKSGVL IARLPYKDIY GVIS

Validated NT Sequence

tngatattac tccgtaaata tccttataag gaagcctagc aataagtact ccactctttt ctcccaattt actacttatg ggcaacattg cataataaca atcttctccc atttcggaca aaagtattct atcaatagtg taaaccgaca cttcactggc aatgtttgat ggaaaagggg gcttagagaa aacatcttta agaacattca aaaatttaga actaaccctg ctggtttcat tatattcttc aaaaggatta actgctatat tgttgggatc cacataaata aaattgcctc ttttataaaa accgaatcta aatctatcaa aactatctgc cacaatatca ttaagcaaat atccggccaa atacccacaa acaagtttat cttctgggga atatgcaggt acaattattg caaaagcctt tttttcgctt tgtttagacc taatagcaac ttctgcggat attccttcag aaagatttga ataccaacct ataaatttta attggttttg cctataatcc tcaacagctt ttttaaaata attggtatta gcctcagaat gaccaaaatc catattattc tcatgtcttg tgctaacaat tactctccct tcaaaatcaa aaaaagcgaa ttcttcaaaa agggtatcat ttttaagatt ggccataaaa ttgtataagt attgtcgata ttttttgctc acctttacag agtcaataac aaattttgga ttttttctta aatctatcaa ttccgactca gagaaatctt ttcctctatt ctcagacatt gcaaattctg atatggtttc aagtgccaaa ttagaagctg caccattgat tatgacatgc agggtgtcta aaaaagattg caaagaaaaa gctgctcttc ttacttgcgc ccttgtaagc tgcttataat aatcttctaa ataacccata tggtggtggt ggtggtgagc catggtatat ct

Expected Protein Sequence

MAHHHHHHGY LEDYYKQLTR AQVRRAAFSL QSFLDTLHVI INGAASNLAL ETISEFAMSE NRGKDFSESE LIDLRKNPKF VIDSVKVSKK YRQYLYNFMA NLKNDTLFEE FAFFDFEGRV IVSTRHENNM DFGHSEANTN YFKKAVEDYR QNQLKFIGWY SNLSEGISAE VAIRSKQSEK KAFAIIVPVY SPEDKLVCGY LAGYLLNDIV ADSFDRFRFG FYKRGNFIYV DPNNIAVNPF EEYNETSRVS SKFLNVLKDV FSKPPFPSNI ASEVSVYTID RILLSEMGED CYYAMLPISS KLGEKSGVLI ARLPYKDIYG VISSLRFQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggttatt tagaagatta ttataagcag cttacaaggg cgcaagtaag aagagcagct ttttctttgc aatctttttt agacaccctg catgtcataa tcaatggtgc agcttctaat ttggcacttg aaaccatatc agaatttgca atgtctgaga atagaggaaa agatttctct gagtcggaat tgatagattt aagaaaaaat ccaaaatttg ttattgactc tgtaaaggtg agcaaaaaat atcgacaata cttatacaat tttatggcca atcttaaaaa tgataccctt tttgaagaat tcgctttttt tgattttgaa gggagagtaa ttgttagcac aagacatgag aataatatgg attttggtca ttctgaggct aataccaatt attttaaaaa agctgttgag gattataggc aaaaccaatt aaaatttata ggttggtatt caaatctttc tgaaggaata tccgcagaag ttgctattag gtctaaacaa agcgaaaaaa aggcttttgc aataattgta cctgtatatt ccccagaaga taaacttgtt tgtgggtatt tggccggata tttgcttaat gatattgtgg cagatagttt tgatagattt agattcggtt tttataaaag aggcaatttt atttatgtgg atcccaacaa tatagcagtt aatccttttg aagaatataa tgaaaccagc agggttagtt ctaaattttt gaatgttctt aaagatgttt tctctaagcc cccttttcca tcaaacattg ccagtgaagt gtcggtttac actattgata gaatactttt gtccgaaatg ggagaagatt gttattatgc aatgttgccc ataagtagta aattgggaga aaagagtgga gtacttattg ctaggcttcc ttataaggat atttacggag taatatctag tctaagattt cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat