BobuA.18861.a

protein-export membrane protein (secD) (BB_0652)

CENTER ID: BobuA.18861.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18861.a.B2.GE39301 soluble domain 27 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1043
RefSeq: NP_212786.1
UniProt: O51596

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKGSKLILI LLVTFFACLL IFPTLKWYFL MSVEDKKISS YSQEALRDYS KKKALNDLVK LKELYNKDPN SSIPASLSYL IPIAKNNYRS SMKIPPNIFT AKTLREGFLT DSDMGEVSLE IYRYYENIKK GKSRIIHLGL DLSGGMSVTI SLDYSSVEKK LGRSLTFAER EDAIYRIMQI LKDRVBRFGL TEPKIVREAG GNKIFLDIPG EKDESRVSTL LSGKGNLTFY VVDDESTSLL HRKILEAGSL FSIPEIQASM NLPDSKQIFP WYVKDSYGVD DESSVRYYVV DASPENSFDG AHIKDAGVSN DPRTGRDTVA FSLDVDGSEK FFKFTQKNVG KSLAVVMEGK IKSVAGIGYA ITGGNVSIQG DSFDKKEAQD LALVFKTAAF PVDIKIDDLR IIGPTLGART IDLGIKASAL ALCLVFLFIC VYYGLSGVVA GFSLVIYNVF LILAILSAFN FTLTLTSIAG LILTMGMAVD INIVIYERIK EEIREGRRFE NAFEAGFKKA FLSIMDANIT TFIAVLFLTL LGTGVIQGFA WSLSVGIVAS LFSSLIFSRF ILEFIISVRK SKFISISWSS KYAKSN
NT Sequence
atgaaaaaag gatctaagct tatattgata ttgttggtga cgttttttgc atgtctttta atatttccga ctttaaaatg gtatttttta atgagcgttg aggataaaaa aataagctca tattcacaag aggccttaag ggattattca aagaaaaaag ccctgaatga tcttgttaag cttaaggaac tgtataataa agatcccaat agcagtattc cagctagttt gtcttattta attccaatag caaaaaataa ttataggtct tcaatgaaaa ttccacctaa tatttttact gctaaaacct tgcgtgaagg atttttgact gattcggata tgggagaagt aagcttagag atttatagat attatgagaa cataaagaag ggcaaaagca gaataataca tcttgggctt gatttgtctg gagggatgag tgttaccatt tctcttgatt attcaagtgt tgaaaaaaaa ttaggtcgtt ctttgacttt tgctgagaga gaggatgcca tttatcgaat aatgcaaatt cttaaagata gggtaratag gttcgggctt acagagccta aaattgtaag agaagccggg ggaaataaaa ttttcttaga tattcctgga gaaaaagatg agagtagagt aagcactctt ttgagcggta aaggcaattt gactttttat gtggttgatg atgagtctac atctcttttg catagaaaaa tattagaagc gggctctctt ttttctattc ctgaaattca ggcaagtatg aaccttccag atagtaaaca aatttttcct tggtatgtta aagattctta tggtgtggat gatgagtcat cagttcgtta ttatgtagtt gatgcaagcc ctgaaaattc atttgatggt gctcacatta aagatgctgg ggtttctaac gatcctagaa caggccgaga tactgttgct tttagccttg atgttgatgg aagtgaaaaa ttttttaaat ttactcaaaa aaatgttgga aagtctttgg ccgttgttat ggaaggtaaa attaagtctg tggcaggaat tggatatgct atcactgggg gcaatgtttc aattcaaggt gactcttttg ataaaaaaga ggctcaagat cttgctttag tgtttaaaac tgcagctttt ccagttgata ttaaaataga tgatttgaga ataatagggc ctactcttgg tgctaggact attgatcttg gcattaaagc ttctgcgctt gctctttgtt tagttttttt gtttatatgt gtttattatg gtttgagtgg tgttgtagct ggattttcac ttgttattta taatgtattt ttaattttag cgatattgtc ggcctttaat tttactttaa ctttaacaag cattgcaggt cttattttga caatgggtat ggccgttgac ataaatatag ttatttatga gagaattaaa gaagaaatta gagaaggcag aagatttgaa aatgcttttg aagctggttt taaaaaggca tttttatcaa ttatggatgc aaatataaca acatttattg cggtgctttt tttaactctt cttgggacag gagttattca aggttttgct tggtctcttt ctgttggaat tgtggcatcc ctttttagta gtttgatttt ttcaagattt attttggaat ttatcatatc tgttagaaaa agcaaattta taagtatatc ttggagttca aaatatgcaa agagtaatta a
Details for BobuA.18861.a.B2.GE39301
HARVESTED ON: data unavailable
SEQUENCED ON: 7/6/2015
EXPECTED MW: 44kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMW YFLMSVEDKK ISSYSQEALR DYSKKKALND LVKLKELYNK DPNSSIPASL SYLIPIAKNN YRSSMKIPPN IFTAKTLREG FLTDSDMGEV SLEIYRYYEN IKKGKSRIIH LGLDLSGGMS VTISLDYSSV EKKLGRSLTF AEREDAIYRI MQILKDRVDR FGLTEPKIVR EAGGNKIFLD IPGEKDESRV STLLSGKGNL TFYVVDDEST SLLHRKILEA GSLFSIPEIQ ASMNLPDSKQ IFPWYVKDSY GVDDESSVRY YVVDASPENS FDGAHIKDAG VSNDPRTGRD TVAFSLDVDG SEKFFKFTQK NVGKSLAVVM EGKIKSVAGI GYAITGGNVS IQGDSFDKKE AQDLALVFKT AAFPVDIKID DLRIIG
Validated NT Sequence
aggccctatt attctcaaat catctatttt aatatcaact ggaaaagctg cagttttaaa cactaaagca agatcttgag cctctttttt atcaaaagag tcaccttgaa ttgaaacatt gcccccagtg atagcatatc caattcctgc cacagactta attttacctt ccataacaac ggccaaagac tttccaacat ttttttgagt aaatttaaaa aatttttcac ttccatcaac atcaaggcta aaagcaacag tatctcggcc tgttctagga tcgttagaaa ccccagcatc tttaatgtga gcaccatcaa atgaattttc agggcttgca tcaactacat aataacgaac tgatgactca tcatccacac cataagaatc tttaacatac caaggaaaaa tttgtttact atctggaagg ttcatacttg cctgaatttc aggaatagaa aaaagagagc ccgcttctaa tatttttcta tgcaaaagag atgtagactc atcatcaacc acataaaaag tcaaattgcc tttaccgctc aaaagagtgc ttactctact ctcatctttt tctccaggaa tatctaagaa aattttattt cccccggctt ctcttacaat tttaggctct gtaagcccga acctatctac cctatcttta agaatttgca ttattcgata aatggcatcc tctctctcag caaaagtcaa agaacgacct aatttttttt caacacttga ataatcaaga gaaatggtaa cactcatccc tccagacaaa tcaagcccaa gatgtattat tctgcttttg cccttcttta tgttctcata atatctataa atctctaagc ttacttctcc catatccgaa tcagtcaaaa atccttcacg caaggtttta gcagtaaaaa tattaggtgg aattttcatt gaagacctat aattattttt tgctattgga attaaataag acaaactagc tggaatactg ctattgggat ctttattata cagttcctta agcttaacaa gatcattcag ggcttttttc tttgaataat cccttaaggc ctcttgtgaa tatgagctta tttttttatc ctcaacgctc attaaaaaat accacatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHWY FLMSVEDKKI SSYSQEALRD YSKKKALNDL VKLKELYNKD PNSSIPASLS YLIPIAKNNY RSSMKIPPNI FTAKTLREGF LTDSDMGEVS LEIYRYYENI KKGKSRIIHL GLDLSGGMSV TISLDYSSVE KKLGRSLTFA EREDAIYRIM QILKDRVBRF GLTEPKIVRE AGGNKIFLDI PGEKDESRVS TLLSGKGNLT FYVVDDESTS LLHRKILEAG SLFSIPEIQA SMNLPDSKQI FPWYVKDSYG VDDESSVRYY VVDASPENSF DGAHIKDAGV SNDPRTGRDT VAFSLDVDGS EKFFKFTQKN VGKSLAVVME GKIKSVAGIG YAITGGNVSI QGDSFDKKEA QDLALVFKTA AFPVDIKIDD LRIIGPTLGA RTIDL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattggtatt ttttaatgag cgttgaggat aaaaaaataa gctcatattc acaagaggcc ttaagggatt attcaaagaa aaaagccctg aatgatcttg ttaagcttaa ggaactgtat aataaagatc ccaatagcag tattccagct agtttgtctt atttaattcc aatagcaaaa aataattata ggtcttcaat gaaaattcca cctaatattt ttactgctaa aaccttgcgt gaaggatttt tgactgattc ggatatggga gaagtaagct tagagattta tagatattat gagaacataa agaagggcaa aagcagaata atacatcttg ggcttgattt gtctggaggg atgagtgtta ccatttctct tgattattca agtgttgaaa aaaaattagg tcgttctttg acttttgctg agagagagga tgccatttat cgaataatgc aaattcttaa agatagggta rataggttcg ggcttacaga gcctaaaatt gtaagagaag ccgggggaaa taaaattttc ttagatattc ctggagaaaa agatgagagt agagtaagca ctcttttgag cggtaaaggc aatttgactt tttatgtggt tgatgatgag tctacatctc ttttgcatag aaaaatatta gaagcgggct ctcttttttc tattcctgaa attcaggcaa gtatgaacct tccagatagt aaacaaattt ttccttggta tgttaaagat tcttatggtg tggatgatga gtcatcagtt cgttattatg tagttgatgc aagccctgaa aattcatttg atggtgctca cattaaagat gctggggttt ctaacgatcc tagaacaggc cgagatactg ttgcttttag ccttgatgtt gatggaagtg aaaaattttt taaatttact caaaaaaatg ttggaaagtc tttggccgtt gttatggaag gtaaaattaa gtctgtggca ggaattggat atgctatcac tgggggcaat gtttcaattc aaggtgactc ttttgataaa aaagaggctc aagatcttgc tttagtgttt aaaactgcag cttttccagt tgatattaaa atagatgatt tgagaataat agggcctact cttggtgcta ggactattga tctttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat