SSGCID: Target Page

BobuA.18857.a

translation initiation factor 3 (infC) (BB_0190)

CENTER ID:	BobuA.18857.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18857.a.B1.GE39300	full length		1	186

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.587
RefSeq:	NP_212324.1
UniProt:	O51208

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MINRNANRDR DRSRSNDKEL KINYRIKARE VRVIFENGTQ EVLSIEDAIK KAKEAGLDLV EVSPNVSPPV CKIIDYGKYK FHQEKRQKEQ KKNQKVIKLK EVRMQPKIDA HDLDFKSKNI LGFLKDGNKV KVTIRFRGRE LAHTYLGYGI LNSILEKVGD VNYVLESAAK MEGKTMFLIV APKFKK

NT Sequence

atgataaata gaaatgccaa tagagatagg gataggtcca gatcaaatga caaggaatta aaaattaatt acagaattaa ggctcgcgag gtaagagtta tttttgaaaa tggaacacaa gaggttttat cgattgaaga tgctattaaa aaagcaaaag aagctggact tgatttggtt gaggtttctc ctaatgtatc tcctccggtt tgcaagatta ttgattatgg aaaatataaa ttccatcaag aaaagcgtca aaaagagcaa aagaaaaatc aaaaagtaat taagcttaag gaagtcagaa tgcagcccaa gatagatgct catgaccttg attttaaatc aaaaaacatt ttaggttttt taaaagatgg caataaggtt aaagttacaa taagatttag aggtcgtgaa cttgctcaca catatttagg atatggtatc ttaaatagca ttcttgaaaa agtaggggat gtgaattatg ttttggaatc tgcggctaaa atggaaggca aaacaatgtt tttaattgta gcgcctaagt ttaaaaaata a

Details for BobuA.18857.a.B1.GE39300

HARVESTED ON:	data unavailable
SEQUENCED ON:	7/6/2015
EXPECTED MW:	23kDa
OBSERVED MW:	0kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Data Unavailable
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI NRNANRDRDR SRSNDKELKI NYRIKAREVR VIFENGTQEV LSIEDAIKKA KEAGLDLVEV SPNVSPPVCK IIDYGKYKFH QEKRQKEQKK NQKVIKLKEV RMQPKIDAHD LDFKSKNILG FLKDGNKVKV TIRFRGRELA HTYLGYGILN SILEKVGDVN YVLESAAKME GKTMFLIVAP KFKK

Validated NT Sequence

atcttactca cttatttttt aaacttaggc gctacaatta aaaacattgt tttgccttcc attttagccg cagattccaa aacataattc acatccccta ctttttcaag aatgctattt aagataccat atcctaaata tgtgtgagca agttcacgac ctctaaatct tattgtaact ttaaccttat tgccatcttt taaaaaacct aaaatgtttt ttgatttaaa atcaaggtca tgagcatcta tcttgggctg cattctgact tccttaagct taattacttt ttgatttttc ttttgctctt tttgacgctt ttcttgatgg aatttatatt ttccataatc aataatcttg caaaccggag gagatacatt aggagaaacc tcaaccaaat caagtccagc ttcttttgct tttttaatag catcttcaat cgataaaacc tcttgtgttc cattttcaaa aataactctt acctcgcgag ccttaattct gtaattaatt tttaattcct tgtcatttga tctggaccta tccctatctc tattggcatt tctatttatc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgataa atagaaatgc caatagagat agggataggt ccagatcaaa tgacaaggaa ttaaaaatta attacagaat taaggctcgc gaggtaagag ttatttttga aaatggaaca caagaggttt tatcgattga agatgctatt aaaaaagcaa aagaagctgg acttgatttg gttgaggttt ctcctaatgt atctcctccg gtttgcaaga ttattgatta tggaaaatat aaattccatc aagaaaagcg tcaaaaagag caaaagaaaa atcaaaaagt aattaagctt aaggaagtca gaatgcagcc caagatagat gctcatgacc ttgattttaa atcaaaaaac attttaggtt ttttaaaaga tggcaataag gttaaagtta caataagatt tagaggtcgt gaacttgctc acacatattt aggatatggt atcttaaata gcattcttga aaaagtaggg gatgtgaatt atgttttgga atctgcggct aaaatggaag gcaaaacaat gtttttaatt gtagcgccta agtttaaaaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat