BobuA.18849.a

valyl-tRNAsynthase (valS) (BB_0738)

CENTER ID: BobuA.18849.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18849.a.B1.GE39232 full length 1 875
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1129
RefSeq: NP_212872.1
UniProt: O51680

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNCRPLEKYD PKAFEDEIYT KWLKNNVFLP DNSLFEKFSM VAPPPNVTGV LHMGHALNFV LQDVLVRYKR MKRHNTLWLF GTDHAGIATQ AVFERHLKKI GKSKDDFERE ELVQEIFKLK DRHRGIIVNQ INKLGASYDH SRERFTLDEN LCKAVNKVFK DLYFKGLIYR GEYLVNLDPG SGSVVSDEEI EYKEVDGKLY FVKYFIDNSS FIEVATTRPE TMFGDTAIAV NPNDERYKSL VGKEVTIPLT TKKIKVIADF YVDSAFGTGA LKVTPAHDPN DFEISKRHNI SKVNILTQDG KLNKNVPLQY QGLSAKDARF KIETELMEKG FLQDVKKHKQ QVGHCYRSGE VIEPYLSTQW FVRMKPLADK ALKALENGEL KFYPKKWENT YKYWLSNIRD WCISRQLVWG HRIPVWYNVD TSELIVSDTD PSLDEKNMGK RFVQDPDVLD TWFSSWLWPF SSLGWPNVDV DFKNYYPTNT LITAYDIIFF WVARMVMAGL EFTGQVPFKD VYITPLLRDK QGKKMSKSLG NGIDPLDIIN EYGSDSLRFT LSFLSVQGQD LNIDAKDFMF GAKFANKVFN ASKFILLNLK NRKILNDLKF NDIDKWLLTS LNSTILGVES SFANYKYNEA SKFVYEFFWN DFCDWYIEIS KIDLNNENVD IQNMAISKLL FFLKKSLLIL HPFIPFVTEK IYSEFAEKED ILALNEYPNF DIANNFQEEF EIFKVLKTFI IAIRTLKSEF NIPASVEIDV ALKFDADFKY EAYFKANESI VKRMINFKNI FYNENYDGML GVAAVGFEIY ADVKSLIDKT KELIRLEKQL EKYKMLNISV SKKLENENFL MNAPKEIVES EKLKFVEFSS LINKINNYII NLKNL
NT Sequence
atgaattgta gacctcttga aaaatatgat cctaaggcat ttgaagatga aatttacact aagtggctta aaaacaatgt ttttttgcca gataattctt tatttgaaaa atttagtatg gttgcgcctc ctcctaatgt tactggcgtg ttgcacatgg ggcatgctct taattttgtt ttgcaagatg ttcttgtaag gtataaaaga atgaaaagac acaatacttt gtggcttttt ggcacagatc atgcaggaat agcaacgcag gctgtttttg aaagacatct taaaaagatt ggtaaaagca aagatgattt tgaaagagaa gagcttgttc aagaaatttt taaattaaaa gataggcata gagggataat tgttaatcag ataaacaaac ttggggcgtc ttatgatcac tcaagagaaa ggtttactct tgatgagaat ctttgtaagg ctgttaacaa ggtttttaag gacttgtatt ttaaggggtt gatttatagg ggtgagtatc ttgttaatct tgatcctgga tctgggagtg ttgttagcga tgaagagatt gaatacaaag aagttgatgg taagctttat tttgttaagt attttattga taactcttct tttattgagg ttgcaacaac taggcctgag acaatgtttg gggatactgc tattgctgtt aatcctaatg atgagagata taagtcttta gttggcaaag aagttacaat tcctttgaca actaaaaaga taaaagttat tgcagatttt tatgttgaca gcgcttttgg cactggggct ttaaaagtta ctcctgcaca cgatcctaat gattttgaaa tttcaaaaag gcacaatatt tctaaggtca atattttaac tcaagatgga aaacttaata aaaatgttcc tttgcaatac caaggattaa gtgcgaaaga tgcaagattt aaaattgaaa cagaattaat ggaaaagggt tttttgcaag atgttaagaa gcataaacaa caggttgggc attgttatcg gtcaggtgag gttattgaac cttatttgtc tactcagtgg tttgtgagaa tgaagccttt agcagacaaa gctttaaagg ctttagagaa tggcgaatta aaattttatc ctaaaaagtg ggaaaataca tataaatatt ggttatcaaa tattagagat tggtgtatat caagacagct tgtttgggga catagaatac cggtttggta caatgttgat acatctgaac ttattgttag tgatactgat ccttctttag atgaaaagaa tatggggaag aggtttgttc aagatccaga tgttcttgat acttggtttt cttcttggct atggcccttt tcttcgcttg gatggcccaa tgttgatgtt gattttaaaa attattatcc aacaaatacc ttgataacag cttacgatat aatatttttt tgggttgcaa gaatggtgat ggcaggatta gaatttacag ggcaagttcc tttcaaagat gtttatataa cacctctttt gcgtgacaaa caaggtaaaa aaatgtcaaa gtctttaggc aatggaatag accctcttga tattattaat gagtatggaa gtgattcttt gcggtttact ttatcctttt tgtctgttca gggtcaagat ttaaatattg acgctaaaga ttttatgttt ggggctaaat ttgcaaacaa agtttttaat gcttccaaat ttattctttt aaatttaaaa aatagaaaaa tattaaatga tttgaaattt aacgacattg acaaatggct gcttacaagc ttaaattcga ctattcttgg tgtagagtct tcttttgcaa attataaata taacgaagct tcaaaatttg tttatgagtt tttttggaat gatttttgtg attggtatat tgaaattagc aaaattgatt taaataatga aaatgttgat attcaaaata tggctatttc taagttgcta ttttttctta aaaaatcatt gttaattttg catccgttta ttccttttgt tacagaaaaa atttattctg aatttgcaga aaaggaagat attttagctt taaatgaata tccaaatttt gatattgcca ataattttca agaagaattt gaaattttta aagtattgaa aacttttatt atagctatta gaacacttaa gagtgaattt aatatacctg ctagtgttga aattgatgtt gctttgaagt ttgatgctga ttttaaatat gaggcatact ttaaggctaa tgaaagcatt gtaaaaagaa tgattaattt taaaaatata ttttacaatg aaaattatga tggcatgctt ggtgtagctg cagttggttt tgaaatttat gcagatgtta agtcattgat agataaaacc aaagagttga taaggcttga aaagcagctt gaaaagtata aaatgctcaa tatttctgtt tcaaagaaac ttgaaaatga aaatttttta atgaatgctc ccaaggaaat tgttgaatct gaaaaattaa aatttgtaga attttcttct ttaattaata aaataaataa ttatattatt aatttaaaaa atctgtaa
Details for BobuA.18849.a.B1.GE39232
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 103kDa
OBSERVED MW: 103kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN CRPLEKYDPK AFEDEIYTKW LKNNVFLPDN SLFEKFSMVA PPPNVTGVLH MGHALNFVLQ DVLVRYKRMK RHNTLWLFGT DHAGIATQAV FERHLKKIGK SKDDFEREEL VQEIFKLKDR HRGIIVNQIN KLGASYDHSR ERFTLDENLC KAVNKVFKDL YFKGLIYRGE YLVNLDPGSG SVVSDEEIEY KEVDGKLYFV KYFIDNSSFI EVATTRPETM FGDTAIAVNP NDERYKSLVG KEVTIPLTTK KIKVIADFYV DSAFGTGALK VTPAHDPNDF EISKRHNISK VNILTQDGKL NKNVPLQYQG LSAKDARFKI ETELMEKGFL QDVKKHKQQV GHCYRSGEVI EPYLSTQWFV RMKPLADKAL KALENGELKF YPKKWENTYK YWLSNIRDWC ISRQLVWGHR IPVWYNVDTS ELIVSDTDPS LDEKNMGKRF VQDPDVLDTW FSSWLWPFSS LGWPNVDVDF KNYYPTNTLI TAYDIIFFWV ARMVMAGLEF TGQVPFKDVY ITPLLRDKQG KKMSKSLGNG IDPLDIINEY GSDSLRFTLS FLSVQGQDLN IDAKDFMFGA KFANKVFNAS KFILLNLKNR KILNDLKFND IDKWLLTSLN STILGVESSF ANYKYNEASK FVYEFFWNDF CDWYIEISKI DLNNENVDIQ NMAISKLLFF LKKSLLILHP FIPFVTEKIY SEFAEKEDIL ALNEYPNFDI ANNFQEEFEI FKVLKTFIIA IRTLKSEFNI PASVEIDVAL KFDADFKYEA YFKANESIVK RMINFKNIFY NENYDGMLGV AAVGFEIYAD VKSLIDKTKE LIRLEKQLEK YKMLNISVSK KLENENFLMN APKEIVESEK LKFVEFSSLI NKINNYIINL KNL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt gtagacctct tgaaaaatat gatcctaagg catttgaaga tgaaatttac actaagtggc ttaaaaacaa tgtttttttg ccagataatt ctttatttga aaaatttagt atggttgcgc ctcctcctaa tgttactggc gtgttgcaca tggggcatgc tcttaatttt gttttgcaag atgttcttgt aaggtataaa agaatgaaaa gacacaatac tttgtggctt tttggcacag atcatgcagg aatagcaacg caggctgttt ttgaaagaca tcttaaaaag attggtaaaa gcaaagatga ttttgaaaga gaagagcttg ttcaagaaat ttttaaatta aaagataggc atagagggat aattgttaat cagataaaca aacttggggc gtcttatgat cactcaagag aaaggtttac tcttgatgag aatctttgta aggctgttaa caaggttttt aaggacttgt attttaaggg gttgatttat aggggtgagt atcttgttaa tcttgatcct ggatctggga gtgttgttag cgatgaagag attgaataca aagaagttga tggtaagctt tattttgtta agtattttat tgataactct tcttttattg aggttgcaac aactaggcct gagacaatgt ttggggatac tgctattgct gttaatccta atgatgagag atataagtct ttagttggca aagaagttac aattcctttg acaactaaaa agataaaagt tattgcagat ttttatgttg acagcgcttt tggcactggg gctttaaaag ttactcctgc acacgatcct aatgattttg aaatttcaaa aaggcacaat atttctaagg tcaatatttt aactcaagat ggaaaactta ataaaaatgt tcctttgcaa taccaaggat taagtgcgaa agatgcaaga tttaaaattg aaacagaatt aatggaaaag ggttttttgc aagatgttaa gaagcataaa caacaggttg ggcattgtta tcggtcaggt gaggttattg aaccttattt gtctactcag tggtttgtga gaatgaagcc tttagcagac aaagctttaa aggctttaga gaatggcgaa ttaaaatttt atcctaaaaa gtgggaaaat acatataaat attggttatc aaatattaga gattggtgta tatcaagaca gcttgtttgg ggacatagaa taccggtttg gtacaatgtt gatacatctg aacttattgt tagtgatact gatccttctt tagatgaaaa gaatatgggg aagaggtttg ttcaagatcc agatgttctt gatacttggt tttcttcttg gctatggccc ttttcttcgc ttggatggcc caatgttgat gttgatttta aaaattatta tccaacaaat accttgataa cagcttacga tataatattt ttttgggttg caagaatggt gatggcagga ttagaattta cagggcaagt tcctttcaaa gatgtttata taacacctct tttgcgtgac aaacaaggta aaaaaatgtc aaagtcttta ggcaatggaa tagaccctct tgatattatt aatgagtatg gaagtgattc tttgcggttt actttatcct ttttgtctgt tcagggtcaa gatttaaata ttgacgctaa agattttatg tttggggcta aatttgcaaa caaagttttt aatgcttcca aatttattct tttaaattta aaaaatagaa aaatattaaa tgatttgaaa tttaacgaca ttgacaaatg gctgcttaca agcttaaatt cgactattct tggtgtagag tcttcttttg caaattataa atataacgaa gcttcaaaat ttgtttatga gtttttttgg aatgattttt gtgattggta tattgaaatt agcaaaattg atttaaataa tgaaaatgtt gatattcaaa atatggctat ttctaagttg ctattttttc ttaaaaaatc attgttaatt ttgcatccgt ttattccttt tgttacagaa aaaatttatt ctgaatttgc agaaaaggaa gatattttag ctttaaatga atatccaaat tttgatattg ccaataattt tcaagaagaa tttgaaattt ttaaagtatt gaaaactttt attatagcta ttagaacact taagagtgaa tttaatatac ctgctagtgt tgaaattgat gttgctttga agtttgatgc tgattttaaa tatgaggcat actttaaggc taatgaaagc attgtaaaaa gaatgattaa ttttaaaaat atattttaca atgaaaatta tgatggcatg cttggtgtag ctgcagttgg ttttgaaatt tatgcagatg ttaagtcatt gatagataaa accaaagagt tgataaggct tgaaaagcag cttgaaaagt ataaaatgct caatatttct gtttcaaaga aacttgaaaa tgaaaatttt ttaatgaatg ctcccaagga aattgttgaa tctgaaaaat taaaatttgt agaattttct tctttaatta ataaaataaa taattatatt attaatttaa aaaatctgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat