BobuA.18845.a

DNA polymerase III subunit alpha (BB_0579)

CENTER ID: BobuA.18845.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18845.a.B2.GE39299 structural domain (2HPI_A) 1 890
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.970
RefSeq: NP_212713.1
UniProt: O51526

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFRSRFIHL HVHSDYSLLD GAAKISDIIS KAKKCNMSHI ALTDHGNLFG AIKFYKEAKK AGIKPIIGIE AYMAKTSKFL KKQDDLGKMS YHLILLAKNE LGYKNLLKLT SISYLEGFYY RPRIDKDDLE KYSEGLICTS ACIGGLIPRL ILANRFEDAK NEILWFKKVF GNDFYLELQR HGIKDQDIVN ERLVKYSREL GVPLTAANDS HYVNREDATA QDIIVCIGTG AKKSDENRLK METNEFYIKS QEEMCELFND LPEALENTVR IAEKCDDFKI TFPGPILPDY QIPVEFNTLG EYLEHLTLEG LKFRYKNLTS KIKDRAFYEL SVIIGMGFEG YFLIVWDFIK FAHDNDIPVG AGRGSGAGSI VAYALRITDI DPLKYNLLFE RFLNPERISM PDFDIDFCFE GRDEIIKYVT NKYGEDKVAQ IITFGTLKPK AVVKDVARVL DIPFAESNEL TKFIPDGPKV SLKEVLDDNS LKECFTSKPV YKELMNAALV LEGMNRHAST HAAGIVISKT PLTDYVPLYK DYKQGSVSTQ YTMDLLEECG LVKMDFLGLK TLTLIKNAEN LIRSVNPDFK IKNIPDNDVK TFNMLGEGRS ASVFQFESEG MQQILKDAKP DSIEDLIALN ALYRPGPMQF IPQFIAAKKG VKRIKYPHPD LKEVLRPTYG VIVYQEQVME VAKIIGGFSL GKADILRRAM GKKKEDEMNE MKVDFLRGAI EKGYDKEIAS EIFELLKPFS GYGFNKSHAA AYSLIAYQTA YLKANYPEYF MAANLTNEIN NNDKLSYYIE ESKAIGINVL KPDINRSFRE FRVTDSGISY GLNGIKNLGG IVVDLIIDER EKNGKYSSFE DFIRRVDDKV INKKFLESAI KSGLFDSLDQ NRKTLFENLD HLIEVVSEDK NNKKLGQNSL FGALESQDPI QQSFNYQTFK EYSYSELLGF EKELLGFYVS GHPLDPYKKA IDSFSSLNVL TDLAAKKDSI VQFSGILNSV KVIQTKRNNA KMAFGVIEDF KGAIDIVVFT ESYERYRNFL LEGNVIGVVG RLTFNRDKFS IVVEKVVNIE RLSEYKINNI HIKFLNNKLN DLQLLNSLKE SISNFEDNSG FSNVYFYLRE NGKDLKLKMN SILNFVPDED KLDKLRKCVI VEDVWVD
NT Sequence
ttgttaaaaa tcaagtttta ttttgataaa ataattttag gtatgagttt taggtctagg tttattcatc ttcatgttca ttcagattat tctcttttgg atggagctgc aaaaatatca gatattatat caaaagcaaa aaaatgcaat atgtcgcata ttgcattaac agatcatggc aatctttttg gagctattaa attttacaaa gaagctaaaa aagcaggaat taagccaata attggcattg aagcctatat ggcaaaaact tctaagtttt taaaaaaaca ggatgatctt ggaaaaatgt cttaccattt aattttgctt gccaagaatg agttgggtta taaaaaccta ttaaagttaa caagtatttc ttatcttgaa gggttttatt atcgtccaag gatagataaa gatgaccttg aaaaatattc agaaggtttg atttgtactt cagcttgcat tggggggctc attccaagac ttattttggc caatagattt gaagatgcca agaatgaaat tctttggttt aaaaaagttt ttggcaatga tttttatctt gagcttcaaa ggcatggtat taaagatcaa gacattgtaa atgaaaggct ggttaagtat tctagagaac ttggagttcc tttaacagca gctaatgatt ctcattatgt taacagagaa gatgcaactg ctcaagacat cattgtttgt attggtactg gtgctaagaa aagcgatgag aatagattaa aaatggaaac caatgaattt tatattaaat ctcaagagga aatgtgtgaa ctttttaatg atttgcccga agctttagaa aatactgtaa ggattgcaga aaagtgtgat gactttaaaa taacttttcc aggtcctatt ttgcctgatt atcaaattcc tgttgaattt aatactcttg gtgaatattt ggaacatctc acacttgagg ggttgaaatt tagatataaa aatttgacaa gcaaaataaa agatagagct ttttatgaat tgagcgtaat aattggaatg ggctttgaag gctatttttt gattgtttgg gattttatta aatttgctca tgataacgat attcctgttg gagctgggcg tggttctggt gctggttcaa ttgtggctta tgctcttagg attactgata ttgatccttt aaagtataat ttgctttttg agagattttt aaatcctgag cgtatttcta tgcctgattt tgatattgat ttttgttttg aaggcagaga tgagattata aaatatgtta ccaacaaata tggagaagat aaggtagctc aaataattac ttttggaacc ttaaagccta aggctgtagt taaggatgtg gctagggttt tagatattcc atttgctgaa tcaaatgaac ttactaagtt tattcctgat ggtcctaaag tttctttaaa agaggtttta gatgacaatt ctttgaaaga gtgttttact agcaagcctg tttataaaga attaatgaat gccgcattgg ttcttgaggg aatgaataga catgcttcaa ctcatgctgc aggaattgtg atttctaaaa cccctttaac cgactatgtg cctctttata aggattataa gcaaggttct gtttctactc aatacacaat ggatttgctt gaagaatgtg ggcttgttaa gatggatttt cttggtttga aaacattgac gttaataaaa aatgcagaaa atcttattag aagtgtaaat ccagatttta aaataaaaaa tattccagat aatgatgtta agacttttaa tatgctagga gaaggaagaa gtgcgtctgt ttttcagttt gaatctgaag gaatgcagca aattctaaaa gacgcaaagc ccgatagcat tgaagattta atagctttaa atgctcttta taggccaggt cctatgcaat ttattcctca atttattgct gctaaaaaag gtgttaagag aattaaatat cctcatcccg atttaaagga agttttaaga ccaacttatg gggttattgt ttatcaagaa caagtaatgg aagttgcaaa aataattgga ggcttttctc ttggcaaggc cgatatttta agacgtgcta tgggtaaaaa gaaagaagac gagatgaatg aaatgaaagt cgacttctta agaggcgcta ttgagaaagg atatgacaaa gaaattgcta gtgaaatttt tgaactttta aagccctttt ctgggtatgg atttaacaaa tcgcatgcag cggcgtattc tttaatagca tatcaaaccg cttatcttaa ggctaattat cctgaatatt ttatggctgc caatttgacc aatgaaatta ataataatga taagctttct tattacatcg aagagtcaaa agctataggc ataaacgttc tcaagcccga tataaatcga tcatttaggg aatttcgtgt aactgattct gggatttctt atgggcttaa tgggattaaa aatcttggag gaattgttgt tgatttaata attgatgaga gagaaaaaaa cggcaaatat agttcttttg aagattttat aagacgtgta gatgataaag taattaataa gaaattttta gaatctgcaa taaaatctgg actttttgat agtttggatc aaaatagaaa aactttattt gaaaatcttg atcatttgat tgaagttgtt tcagaagata aaaataataa aaaacttggt caaaacagct tatttggtgc tcttgaaagt caagatccaa ttcagcaaag ttttaattat caaactttta aagagtattc ttattctgag cttttaggat ttgaaaaaga gcttttagga ttttatgtgt cgggtcatcc tcttgatcct tataaaaagg caattgacag tttttccagt ttaaatgttt taacagatct tgctgccaaa aaagatagca ttgttcaatt ttctggaatt ttaaattcag taaaagttat tcaaactaaa agaaataatg caaaaatggc tttcggcgtt atagaagatt ttaaaggcgc aatagatatt gtagttttta cagaaagcta tgaaagatat agaaattttt tacttgaagg caatgttatt ggggttgtag gtaggcttac gtttaacaga gataaatttt caattgtggt tgaaaaagtt gtaaatattg agagactttc cgaatataaa ataaataata ttcatattaa gtttttaaat aataaattaa atgacctaca attacttaat tctttaaagg aaagtataag taattttgag gacaattctg gattttcaaa tgtttatttt tatttaaggg aaaatggtaa agatttgaaa ttaaaaatga attcaatttt aaattttgtg ccagatgaag ataagcttga taagttgaga aagtgtgtga tagttgaaga tgtttgggtt gattag
Details for BobuA.18845.a.B2.GE39299
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 102kDa
OBSERVED MW: 102kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS FRSRFIHLHV HSDYSLLDGA AKISDIISKA KKCNMSHIAL TDHGNLFGAI KFYKEAKKAG IKPIIGIEAY MAKTSKFLKK QDDLGKMSYH LILLAKNELG YKNLLKLTSI SYLEGFYYRP RIDKDDLEKY SEGLICTSAC IGGLIPRLIL ANRFEDAKNE ILWFKKVFGN DFYLELQRHG IKDQDIVNER LVKYSRELGV PLTAANDSHY VNREDATAQD IIVCIGTGAK KSDENRLKME TNEFYIKSQE EMCELFNDLP EALENTVRIA EKCDDFKITF PGPILPDYQI PVEFNTLGEY LEHLTLEGLK FRYKNLTSKI KDRAFYELSV IIGMGFEGYF LIVWDFIKFA HDNDIPVGAG RGSGAGSIVA YALRITDIDP LKYNLLFERF LNPERISMPD FDIDFCFEGR DEIIKYVTNK YGEDKVAQII TFGTLKPKAV VKDVARVLDI PFAESNELTK FIPDGPKVSL KEVLDDNSLK ECFTSKPVYK ELMNAALVLE GMNRHASTHA AGIVISKTPL TDYVPLYKDY KQGSVSTQYT MDLLEECGLV KMDFLGLKTL TLIKNAENLI RSVNPDFKIK NIPDNDVKTF NMLGEGRSAS VFQFESEGMQ QILKDAKPDS IEDLIALNAL YRPGPMQFIP QFIAAKKGVK RIKYPHPDLK EVLRPTYGVI VYQEQVMEVA KIIGGFSLGK ADILRRAMGK KKEDEMNEMK VDFLRGAIEK GYDKEIASEI FELLKPFSGY GFNKSHAAAY SLIAYQTAYL KANYPEYFMA ANLTNEINNN DKLSYYIEES KAIGINVLKP DINRSFREFR VTDSGISYGL NGIKNLGGIV VDLIIDEREK NGKYSSFEDF IRRVDDKVIN KKFLESAIKS GLFDSLDQNR KTLFENLD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgttaa aaatcaagtt ttattttgat aaaataattt taggtatgag ttttaggtct aggtttattc atcttcatgt tcattcagat tattctcttt tggatggagc tgcaaaaata tcagatatta tatcaaaagc aaaaaaatgc aatatgtcgc atattgcatt aacagatcat ggcaatcttt ttggagctat taaattttac aaagaagcta aaaaagcagg aattaagcca ataattggca ttgaagccta tatggcaaaa acttctaagt ttttaaaaaa acaggatgat cttggaaaaa tgtcttacca tttaattttg cttgccaaga atgagttggg ttataaaaac ctattaaagt taacaagtat ttcttatctt gaagggtttt attatcgtcc aaggatagat aaagatgacc ttgaaaaata ttcagaaggt ttgatttgta cttcagcttg cattgggggg ctcattccaa gacttatttt ggccaataga tttgaagatg ccaagaatga aattctttgg tttaaaaaag tttttggcaa tgatttttat cttgagcttc aaaggcatgg tattaaagat caagacattg taaatgaaag gctggttaag tattctagag aacttggagt tcctttaaca gcagctaatg attctcatta tgttaacaga gaagatgcaa ctgctcaaga catcattgtt tgtattggta ctggtgctaa gaaaagcgat gagaatagat taaaaatgga aaccaatgaa ttttatatta aatctcaaga ggaaatgtgt gaacttttta atgatttgcc cgaagcttta gaaaatactg taaggattgc agaaaagtgt gatgacttta aaataacttt tccaggtcct attttgcctg attatcaaat tcctgttgaa tttaatactc ttggtgaata tttggaacat ctcacacttg aggggttgaa atttagatat aaaaatttga caagcaaaat aaaagataga gctttttatg aattgagcgt aataattgga atgggctttg aaggctattt tttgattgtt tgggatttta ttaaatttgc tcatgataac gatattcctg ttggagctgg gcgtggttct ggtgctggtt caattgtggc ttatgctctt aggattactg atattgatcc tttaaagtat aatttgcttt ttgagagatt tttaaatcct gagcgtattt ctatgcctga ttttgatatt gatttttgtt ttgaaggcag agatgagatt ataaaatatg ttaccaacaa atatggagaa gataaggtag ctcaaataat tacttttgga accttaaagc ctaaggctgt agttaaggat gtggctaggg ttttagatat tccatttgct gaatcaaatg aacttactaa gtttattcct gatggtccta aagtttcttt aaaagaggtt ttagatgaca attctttgaa agagtgtttt actagcaagc ctgtttataa agaattaatg aatgccgcat tggttcttga gggaatgaat agacatgctt caactcatgc tgcaggaatt gtgatttcta aaaccccttt aaccgactat gtgcctcttt ataaggatta taagcaaggt tctgtttcta ctcaatacac aatggatttg cttgaagaat gtgggcttgt taagatggat tttcttggtt tgaaaacatt gacgttaata aaaaatgcag aaaatcttat tagaagtgta aatccagatt ttaaaataaa aaatattcca gataatgatg ttaagacttt taatatgcta ggagaaggaa gaagtgcgtc tgtttttcag tttgaatctg aaggaatgca gcaaattcta aaagacgcaa agcccgatag cattgaagat ttaatagctt taaatgctct ttataggcca ggtcctatgc aatttattcc tcaatttatt gctgctaaaa aaggtgttaa gagaattaaa tatcctcatc ccgatttaaa ggaagtttta agaccaactt atggggttat tgtttatcaa gaacaagtaa tggaagttgc aaaaataatt ggaggctttt ctcttggcaa ggccgatatt ttaagacgtg ctatgggtaa aaagaaagaa gacgagatga atgaaatgaa agtcgacttc ttaagaggcg ctattgagaa aggatatgac aaagaaattg ctagtgaaat ttttgaactt ttaaagccct tttctgggta tggatttaac aaatcgcatg cagcggcgta ttctttaata gcatatcaaa ccgcttatct taaggctaat tatcctgaat attttatggc tgccaatttg accaatgaaa ttaataataa tgataagctt tcttattaca tcgaagagtc aaaagctata ggcataaacg ttctcaagcc cgatataaat cgatcattta gggaatttcg tgtaactgat tctgggattt cttatgggct taatgggatt aaaaatcttg gaggaattgt tgttgattta ataattgatg agagagaaaa aaacggcaaa tatagttctt ttgaagattt tataagacgt gtagatgata aagtaattaa taagaaattt ttagaatctg caataaaatc tggacttttt gattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat