SSGCID: Target Page

BobuA.18708.a

isoleucyl-tRNAsynthase (ileS) (BB_0833)

CENTER ID:	BobuA.18708.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18708.a.B2.GE39231	structural domain (1ILE_A)		1	821

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.1225
RefSeq:	NP_212967.1
UniProt:	O51773

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFKKVENKAN FPKIEEKILK FWNDNKIFEK SIKQREGCEE FTFYDGPPFA TGLPHFGHFV PNTIKDIIPR YQTMQGKYVK RNFGWDTHGL PVEYEVEKKL GISGKYEIEN YGIENFNKEC RKIVLRYTEE WKNIILRLGR WVDFEKGYKT MDISFMESVW WVFKNLYEKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK EVNDPSLTIK FKIKDKNEYL LVWTTTPWTL PSNLGIAVGQ EIEYSKIFDK TKEEILILGS KKLNSYYDDE NSYTIIEKFK GSKLEGIEYE PIFNYFLEQK DKGAFKVHTA DYVTTDDGTG IVHIAPFGEE DYRILKKHTN VDIIDPLDAE CKFTNQVKDF KGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNC PIIYRPISSW FVNVEKIKTK LLEVNEKINW MPAHLKKGRF GKWLENAKDW AISRNRFWGN PIPIWICSKT GKKICIGSKK ELENLSGQKI EDLHKDQIDK ITWPSKDGGK FIRTSEVLDC WFESGAMPYA SNHYPFTNEI NFKNIFPADF IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSFK NYTDPMQVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI WNAYSFFTTY AIIDKFKPPK NLSLAKNNNL DKWIISELES LKKILNTEID KYNLTKSIES LLEFIDKLNN WYIRRSRRRF WKSENDKDKN DAYETLYYAI KTLMILLAPF IPFITEEIYQ NLKTDEDKQS IHLNDYPKAN ENFINKTIEE KINLARKITS MARSLRSLHN IKIRMPISTI YIVTKNQNEQ NMLMEMQEII LDEINAKEMK IKANEEELIT YKAKANFKEL GKKLGKDMKA VSTEISKLKN EDIIKIINGT SYEIKVANAK HYLSLNDIIL EREEKENLKV INEESITIGI DSLITKELYL EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENNETLKEML NKFEKYIKTE TLALNIILNK SKLEKKINLA DDIFTLIGIE KC

NT Sequence

atgtttaaaa aagtagaaaa caaggcaaat tttcctaaaa tagaagaaaa aatattaaaa ttttggaatg acaataagat ctttgaaaaa tcaataaagc agagagaagg atgtgaagaa tttacatttt atgacggacc gccttttgca acaggacttc ctcattttgg acattttgtt ccaaacacaa taaaagacat aattccaaga tatcaaacaa tgcaaggcaa gtatgttaaa agaaattttg gatgggatac tcacggacta cctgttgaat acgaagtaga aaaaaaattg ggaatttctg gaaaatacga aatagaaaat tatggcattg aaaattttaa caaagaatgc agaaaaatag tacttagata tacagaagaa tggaaaaata taatcttgag acttggacga tgggtagatt ttgaaaaggg ttacaaaacc atggatataa gcttcatgga atccgtgtgg tgggtattta aaaatcttta tgaaaaaggt ttaatctacg aaagttacta tgtactaccc tattccccaa agcttgcaac tccgctttca aatttcgaag tgaatcttgg agaatataaa gaagtcaatg acccatcatt aacaataaaa tttaaaataa aagataaaaa cgaatactta ctagtgtgga caaccacccc ctggacattg ccctcaaacc ttggaattgc agtaggacaa gaaatagaat attctaaaat ttttgacaaa acaaaagaag agattttaat acttggatca aaaaagctta atagctatta cgatgatgaa aattcatata ctattataga aaaattcaaa ggcagcaagc ttgaaggcat agaatatgaa cctattttta actacttttt agaacaaaaa gataaggggg ctttcaaggt acacacagct gattatgtta caactgacga tggaacagga attgttcata ttgctccttt tggagaagaa gactacagaa tactcaaaaa acacacaaat gtcgatataa tagacccctt agatgctgaa tgtaaattta caaatcaggt aaaagatttt aaaggacttt ttgtaaaaga tgctgataaa aaaataatag aaaacctaaa attacgcaat tttttattca aaagagaaaa ttatctacac aggtatccat tttgttatag aacaaactgc ccaattattt acagaccaat aagttcgtgg tttgtaaatg tagaaaaaat aaaaaccaaa cttttagagg taaatgaaaa aattaattgg atgccagccc atttaaaaaa aggaagattt ggaaaatggt tagaaaatgc aaaagattgg gcaataagca gaaacagatt ttggggaaat ccaattccaa tttggatatg ctcaaaaaca ggaaaaaaaa tttgcattgg atcaaaaaaa gagcttgaaa acctatctgg ccaaaaaatc gaagacttac ataaagacca aatagataaa ataacctggc caagcaaaga cggtggcaaa tttatcagaa caagcgaggt tctcgattgt tggtttgaat ctggagcaat gccttacgca agcaaccatt atccattcac aaatgaaatt aattttaaaa atatatttcc tgctgacttt attgcagaag gtctagatca aacaagagga tggttttata ctcttacaat cctgggaact gctctttttg aaaacacagc attcaaaaac gttattgtaa atggacttgt gctttcaagc gatggaagaa aaatgtcaaa atcctttaaa aattatacag acccaatgca agtaataaac accttcggag ctgatgcttt aaggctttat ttaataatga gccctgtagt taaagctgat gatttaaaat atagcgacaa tggagtaaga gacgttctta aaaatataat aatacccatt tggaacgctt attcattttt cacaacttat gcaataattg ataaattcaa acctccaaaa aatctcagcc tggctaaaaa caataacctt gacaaatgga tcataagcga acttgaaagt ctaaaaaaaa tactaaatac agaaatagac aaatacaatc taacaaaatc aatagaatct ttacttgaat ttatagataa attaaacaat tggtacataa gaagatcaag gcgaagattt tggaaatcag aaaacgataa agacaaaaat gatgcctacg aaacattata ttatgcaatc aaaactttaa tgattttact tgcacctttt attccattta taacagaaga gatttatcaa aatttaaaaa ctgatgaaga caaacaatca atacacctta acgattatcc aaaagcaaat gaaaatttca ttaacaaaac aattgaagag aaaataaatc tcgcaagaaa aataacttca atggcaagat cactcagatc attgcacaat ataaaaatac gcatgcctat tagtacgata tatatcgtca caaaaaatca aaatgaacaa aatatgctaa tggaaatgca agaaataata ttagatgaaa taaatgcaaa agaaatgaaa ataaaagcta acgaagagga gcttataact tacaaagcaa aagcaaactt taaagaactt gggaaaaagc ttggaaaaga tatgaaagcg gtatctactg aaattagcaa gctaaaaaat gaagacataa taaaaataat aaatggaaca tcctacgaga taaaagtagc caatgcaaag cattatttat cattaaatga tataatatta gaaagagaag aaaaagagaa cttaaaagta ataaatgaag aatccattac aataggaata gactcactaa tcactaaaga gttgtacttg gaagggctga caagagaatt tgtaaggcaa atacaaaatt taagaaaaga aaaaaatttt gatgttagcg atagaataaa tttatacata gaaaataatg aaactttgaa agaaatgcta aataaatttg aaaaatacat taaaactgaa acattagcct taaatatcat attaaacaaa agtaagctag aaaaaaaaat aaaccttgcc gatgacatat ttacactaat aggaattgaa aaatgttaa

Details for BobuA.18708.a.B2.GE39231

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/5/2015
EXPECTED MW:	98kDa
OBSERVED MW:	57kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMF KKVENKANFP KIEEKILKFW NDNKIFEKSI KQREGCEEFT FYDGPPFATG LPHFGHFVPN TIKDIIPRYQ TMQGKYVKRN FGWDTHGLPV EYEVEKKLGI SGKYEIENYG IENFNKECRK IVLRYTEEWK NIILRLGRWV DFEKGYKTMD ISFMESVWWV FKNLYEKGLI YESYYVLPYS PKLATPLSNF EVNLGEYKEV NDPSLTIKFK IKDKNEYLLV WTTTPWTLPS NLGIAVGQEI EYSKIFDKTK EEILILGSKK LNSYYDDENS YTIIEKFKGS KLEGIEYEPI FNYFLEQKDK GAFKVHTADY VTTDDGTGIV HIAPFGEEDY RILKKHTNVD IIDPLDAECK FTNQVKDFKG LFVKDADKKI IENLKLRNFL FKRENYLHRY PFCYRTNCPI IYRPISSWFV NVEKIKTKLL EVNEKINWMP AHLKKGRFGK WLENAKDWAI SRNRFWGNPI PIWICSKTGK KICIGSKKEL ENLSGQKIED LHKDQIDKIT WPSKDGGKFI RTSEVLDCWF ESGAMPYASN HYPFTNEINF KNIFPADFIA EGLDQTRGWF YTLTILGTAL FENTAFKNVI VNGLVLSSDG RKMSKSFKNY TDPMQVINTF GADALRLYLI MSPVVKADDL KYSDNGVRDV LKNIIIPIWN AYSFFTTYAI IDKFKPPKNL SLAKNNNLDK WIISELESLK KILNTEIDKY NLTKSIESLL EFIDKLNNWY IRRSRRRFWK SENDKDKNDA YETLYYAIKT LMILLAPFIP FITEEIYQNL KTDEDKQSIH LNDYPKANEN FINKTIEEKI NLARKITSMA RSLRSLHNI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta aaaaagtaga aaacaaggca aattttccta aaatagaaga aaaaatatta aaattttgga atgacaataa gatctttgaa aaatcaataa agcagagaga aggatgtgaa gaatttacat tttatgacgg accgcctttt gcaacaggac ttcctcattt tggacatttt gttccaaaca caataaaaga cataattcca agatatcaaa caatgcaagg caagtatgtt aaaagaaatt ttggatggga tactcacgga ctacctgttg aatacgaagt agaaaaaaaa ttgggaattt ctggaaaata cgaaatagaa aattatggca ttgaaaattt taacaaagaa tgcagaaaaa tagtacttag atatacagaa gaatggaaaa atataatctt gagacttgga cgatgggtag attttgaaaa gggttacaaa accatggata taagcttcat ggaatccgtg tggtgggtat ttaaaaatct ttatgaaaaa ggtttaatct acgaaagtta ctatgtacta ccctattccc caaagcttgc aactccgctt tcaaatttcg aagtgaatct tggagaatat aaagaagtca atgacccatc attaacaata aaatttaaaa taaaagataa aaacgaatac ttactagtgt ggacaaccac cccctggaca ttgccctcaa accttggaat tgcagtagga caagaaatag aatattctaa aatttttgac aaaacaaaag aagagatttt aatacttgga tcaaaaaagc ttaatagcta ttacgatgat gaaaattcat atactattat agaaaaattc aaaggcagca agcttgaagg catagaatat gaacctattt ttaactactt tttagaacaa aaagataagg gggctttcaa ggtacacaca gctgattatg ttacaactga cgatggaaca ggaattgttc atattgctcc ttttggagaa gaagactaca gaatactcaa aaaacacaca aatgtcgata taatagaccc cttagatgct gaatgtaaat ttacaaatca ggtaaaagat tttaaaggac tttttgtaaa agatgctgat aaaaaaataa tagaaaacct aaaattacgc aattttttat tcaaaagaga aaattatcta cacaggtatc cattttgtta tagaacaaac tgcccaatta tttacagacc aataagttcg tggtttgtaa atgtagaaaa aataaaaacc aaacttttag aggtaaatga aaaaattaat tggatgccag cccatttaaa aaaaggaaga tttggaaaat ggttagaaaa tgcaaaagat tgggcaataa gcagaaacag attttgggga aatccaattc caatttggat atgctcaaaa acaggaaaaa aaatttgcat tggatcaaaa aaagagcttg aaaacctatc tggccaaaaa atcgaagact tacataaaga ccaaatagat aaaataacct ggccaagcaa agacggtggc aaatttatca gaacaagcga ggttctcgat tgttggtttg aatctggagc aatgccttac gcaagcaacc attatccatt cacaaatgaa attaatttta aaaatatatt tcctgctgac tttattgcag aaggtctaga tcaaacaaga ggatggtttt atactcttac aatcctggga actgctcttt ttgaaaacac agcattcaaa aacgttattg taaatggact tgtgctttca agcgatggaa gaaaaatgtc aaaatccttt aaaaattata cagacccaat gcaagtaata aacaccttcg gagctgatgc tttaaggctt tatttaataa tgagccctgt agttaaagct gatgatttaa aatatagcga caatggagta agagacgttc ttaaaaatat aataataccc atttggaacg cttattcatt tttcacaact tatgcaataa ttgataaatt caaacctcca aaaaatctca gcctggctaa aaacaataac cttgacaaat ggatcataag cgaacttgaa agtctaaaaa aaatactaaa tacagaaata gacaaataca atctaacaaa atcaatagaa tctttacttg aatttataga taaattaaac aattggtaca taagaagatc aaggcgaaga ttttggaaat cagaaaacga taaagacaaa aatgatgcct acgaaacatt atattatgca atcaaaactt taatgatttt acttgcacct tttattccat ttataacaga agagatttat caaaatttaa aaactgatga agacaaacaa tcaatacacc ttaacgatta tccaaaagca aatgaaaatt tcattaacaa aacaattgaa gagaaaataa atctcgcaag aaaaataact tcaatggcaa gatcactcag atcattgcac aatatatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t