BobuA.18681.a

prolyl-tRNAsynthase (proS) (BB_0402)

CENTER ID: BobuA.18681.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18681.a.B1.GE39230 full length 1 488
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.796
RefSeq: NP_212536.1
UniProt: O51363

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDFIASKED DYSKWYLDIV QKAKLADYSP VKGCMVIMPY GYSIWSKIQS ILDKKFKETG HENAYFPMLI PYSFLEREKD HIDGFSPEFA IIKDAGGESL AEPLVLRPTS ETIIWNMYSK WIKSYRDLPL KINQWANVVR WEKRTRPFLR TTEFLWQEGH TAHATEEEAL EETLLILDVY KRFIEDYLAI PVFCGKKSEK EKFAGAVSTY SIEALMQDKK ALQAATSHYL GLNFAKAFDV KFQDKDGKMR HVFASSWGVS TRLIGALIMV HSDEKGLVLP PRIAPIEIIV IPIFKKEDEI NKKILDYSDC VVDALKKAEF RVEIDKDVRS SPGFRFSSAE FKGIPIRLEV GINDVLLNSV TISRRDKDRK FKYQISLDSL ISKVKVELDL MQKDLFQRAL NFRILNTKEI FRSSKDSYET FKAYVNDYSG FVLSCWCGSL NCENIIKNET KATIRCIPDD FKARDLTGMT CIYCSSKAKY FVLFAKSY
NT Sequence
atgagtgatt ttatagcatc aaaagaagat gattattcta aatggtattt agatatagtc cagaaagcaa aacttgctga ttacagtcct gtaaaaggat gtatggtaat tatgccttat gggtattcta tttggagtaa aattcagagt atacttgata aaaaatttaa agagacggga cacgagaatg catatttccc tatgcttatt ccttatagtt ttttagaaag agaaaaggat catattgatg gattttcacc cgagtttgct attattaagg atgctggtgg agagagtttg gcagagcctt tggttttaag gcctacctct gagacaatta tttggaatat gtatagtaag tggattaagt cttacagaga tcttcctctt aaaattaatc aatgggcaaa tgttgttcgt tgggaaaaga gaacaaggcc ttttttgcgc actaccgaat ttctatggca agaaggacat actgctcatg ctaccgaaga ggaggcatta gaagaaactt tacttatttt agatgtatat aaaagattca tagaagacta tttggccatt ccggtgtttt gtggtaaaaa atctgaaaag gaaaaatttg cgggggctgt ttctacttat tcaattgagg cattaatgca agataaaaaa gcgcttcaag ccgctacatc ccattattta ggtttaaatt ttgcaaaggc atttgatgta aaatttcaag acaaagatgg caagatgcgg catgtatttg ctagtagctg gggtgtttct accagattga ttggtgcttt gattatggtt cattctgatg agaaaggttt agttttgccg cctcgcattg ctccaataga aattattgtt attcctattt ttaaaaaaga agatgagatt aataaaaaaa ttttagatta ttctgattgt gttgtggatg ctttaaaaaa agcagaattt agggttgaaa ttgataagga cgttagaagt tctccgggat ttagattttc atctgccgag tttaaaggaa ttccaatacg ccttgaagtg gggataaatg atgtcctttt aaattccgtt actatttcaa gaagagataa agacagaaaa tttaagtatc aaatatcact tgattctctt ataagcaagg ttaaggtaga gcttgatttg atgcaaaaag atttatttca aagagcatta aattttagga tcttgaatac taaggagatt tttagaagca gtaaggatag ttatgagaca ttcaaagcct atgtgaatga ttattctgga tttgtgcttt cttgttggtg tggcagtttg aattgtgaaa atattattaa aaatgaaact aaagccacaa taagatgtat ccccgatgat tttaaggcca gagatttaac aggcatgact tgtatttatt gttcatctaa agctaaatat tttgttttat ttgccaaatc ctattaa
Details for BobuA.18681.a.B1.GE39230
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS DFIASKEDDY SKWYLDIVQK AKLADYSPVK GCMVIMPYGY SIWSKIQSIL DKKFKETGHE NAYFPMLIPY SFLEREKDHI DGFSPEFAII KDAGGESLAE PLVLRPTSET IIWNMYSKWI KSYRDLPLKI NQWANVVRWE KRTRPFLRTT EFLWQEGHTA HATEEEALEE TLLILDVYKR FIEDYLAIPV FCGKKSEKEK FAGAVSTYSI EALMQDKKAL QAATSHYLGL NFAKAFDVKF QDKDGKMRHV FASSWGVSTR LIGALIMVHS DEKGLVLPPR IAPIEIIVIP IFKKEDEINK KILDYSDCVV DALKKAEFRV EIDKDVRSSP GFRFSSAEFK GIPIRLEVGI NDVLLNSVTI SRRDKDRKFK YQISLDSLIS KVKVELDLMQ KDLFQRALNF RILNTKEIFR SSKDSYETFK AYVNDYSGFV LSCWCGSLNC ENIIKNETKA TIRCIPDDFK ARDLXXMTCI YCSSK
Validated NT Sequence
gctttagatg aacaataaat acaagtcatg cnnnttaaat ctctggcctt aaaatcatcg gggatacatc ttattgtggc tttagtttca tttttaataa tattttcaca attcaaactg ccacaccaac aagaaagcac aaatccagaa taatcattca cataggcttt gaatgtctca taactatcct tactgcttct aaaaatctcc ttagtattca agatcctaaa atttaatgct ctttgaaata aatctttttg catcaaatca agctctacct taaccttgct tataagagaa tcaagtgata tttgatactt aaattttctg tctttatctc ttcttgaaat agtaacggaa tttaaaagga catcatttat ccccacttca aggcgtattg gaattccttt aaactcggca gatgaaaatc taaatcccgg agaacttcta acgtccttat caatttcaac cctaaattct gcttttttta aagcatccac aacacaatca gaataatcta aaattttttt attaatctca tcttcttttt taaaaatagg aataacaata atttctattg gagcaatgcg aggcggcaaa actaaacctt tctcatcaga atgaaccata atcaaagcac caatcaatct ggtagaaaca ccccagctac tagcaaatac atgccgcatc ttgccatctt tgtcttgaaa ttttacatca aatgcctttg caaaatttaa acctaaataa tgggatgtag cggcttgaag cgctttttta tcttgcatta atgcctcaat tgaataagta gaaacagccc ccgcaaattt ttccttttca gattttttac cacaaaacac cggaatggcc aaatagtctt ctatgaatct tttatataca tctaaaataa gtaaagtttc ttctaatgcc tcctcttcgg tagcatgagc agtatgtcct tcttgccata gaaattcggt agtgcgcaaa aaaggccttg ttctcttttc ccaacgaaca acatttgccc attgattaat tttaagagga agatctctgt aagacttaat ccacttacta tacatattcc aaataattgt ctcagaggta ggccttaaaa ccaaaggctc tgccaaactc tctccaccag catccttaat aatagcaaac tcgggtgaaa atccatcaat atgatccttt tctctttcta aaaaactata aggaataagc atagggaaat atgcattctc gtgtcccgtc tctttaaatt ttttatcaag tatactctga attttactcc aaatagaata cccataaggc ataattacca tacatccttt tacaggactg taatcagcaa gttttgcttt ctggactata tctaaatacc atttagaata atcatcttct tttgatgcta taaaatcact catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS DFIASKEDDY SKWYLDIVQK AKLADYSPVK GCMVIMPYGY SIWSKIQSIL DKKFKETGHE NAYFPMLIPY SFLEREKDHI DGFSPEFAII KDAGGESLAE PLVLRPTSET IIWNMYSKWI KSYRDLPLKI NQWANVVRWE KRTRPFLRTT EFLWQEGHTA HATEEEALEE TLLILDVYKR FIEDYLAIPV FCGKKSEKEK FAGAVSTYSI EALMQDKKAL QAATSHYLGL NFAKAFDVKF QDKDGKMRHV FASSWGVSTR LIGALIMVHS DEKGLVLPPR IAPIEIIVIP IFKKEDEINK KILDYSDCVV DALKKAEFRV EIDKDVRSSP GFRFSSAEFK GIPIRLEVGI NDVLLNSVTI SRRDKDRKFK YQISLDSLIS KVKVELDLMQ KDLFQRALNF RILNTKEIFR SSKDSYETFK AYVNDYSGFV LSCWCGSLNC ENIIKNETKA TIRCIPDDFK ARDLTGMTCI YCSSKAKYFV LFAKSY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg attttatagc atcaaaagaa gatgattatt ctaaatggta tttagatata gtccagaaag caaaacttgc tgattacagt cctgtaaaag gatgtatggt aattatgcct tatgggtatt ctatttggag taaaattcag agtatacttg ataaaaaatt taaagagacg ggacacgaga atgcatattt ccctatgctt attccttata gttttttaga aagagaaaag gatcatattg atggattttc acccgagttt gctattatta aggatgctgg tggagagagt ttggcagagc ctttggtttt aaggcctacc tctgagacaa ttatttggaa tatgtatagt aagtggatta agtcttacag agatcttcct cttaaaatta atcaatgggc aaatgttgtt cgttgggaaa agagaacaag gccttttttg cgcactaccg aatttctatg gcaagaagga catactgctc atgctaccga agaggaggca ttagaagaaa ctttacttat tttagatgta tataaaagat tcatagaaga ctatttggcc attccggtgt tttgtggtaa aaaatctgaa aaggaaaaat ttgcgggggc tgtttctact tattcaattg aggcattaat gcaagataaa aaagcgcttc aagccgctac atcccattat ttaggtttaa attttgcaaa ggcatttgat gtaaaatttc aagacaaaga tggcaagatg cggcatgtat ttgctagtag ctggggtgtt tctaccagat tgattggtgc tttgattatg gttcattctg atgagaaagg tttagttttg ccgcctcgca ttgctccaat agaaattatt gttattccta tttttaaaaa agaagatgag attaataaaa aaattttaga ttattctgat tgtgttgtgg atgctttaaa aaaagcagaa tttagggttg aaattgataa ggacgttaga agttctccgg gatttagatt ttcatctgcc gagtttaaag gaattccaat acgccttgaa gtggggataa atgatgtcct tttaaattcc gttactattt caagaagaga taaagacaga aaatttaagt atcaaatatc acttgattct cttataagca aggttaaggt agagcttgat ttgatgcaaa aagatttatt tcaaagagca ttaaatttta ggatcttgaa tactaaggag atttttagaa gcagtaagga tagttatgag acattcaaag cctatgtgaa tgattattct ggatttgtgc tttcttgttg gtgtggcagt ttgaattgtg aaaatattat taaaaatgaa actaaagcca caataagatg tatccccgat gattttaagg ccagagattt aacaggcatg acttgtattt attgttcatc taaagctaaa tattttgttt tatttgccaa atcctattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at