SSGCID: Target Page

BobuA.18548.a

Borrelia ORF-A superfamily (BB_F26)

CENTER ID:	BobuA.18548.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18548.a.B1.GE38705	full length		1	366

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.1315
RefSeq:	NP_045459.1
UniProt:	O51031

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKSIKTSTNK YQHKLIILIS TLNYVNSNLE QYTQNDILYY FNNNIRRNCQ KEVKLKTLQN YLYKLEKVFK VTSNYHRHLG INMGTEVHYK LKYPKKVCYH LINKHFKEIK KKKYKSRVDS YFNKNCIENS SVKNRECFYN ISNNKEEKRN SRKSIEKLQI RKYIEKCNFK SNIFSLILNL GLKKDATIEV CKMIKKAENL IEKNIFEKLN VVKVIEVNKL KNKQQKLKRI LKEVKIKLEN ENYKSEQLEI EIKDIYEQYK NKPHFIIEKN KYEDLEKITE KLKNKFKQEK INIKENDTNI RNNIFNILIE QLRHKIKIEI LIPILKDYLD KQKRLEYSKV FSNYYYYEIL ETVEPNKWCS QLEACK

NT Sequence

atgaaaagta taaaaacctc tacaaataaa taccaacaca aattaatcat tctaatatca acactgaatt atgtaaattc aaatcttgaa caatacactc aaaatgacat actttattac tttaataata atataagaag aaattgtcaa aaagaagtta agctaaaaac actacaaaac tatctttaca aattagaaaa ggtatttaaa gtaactagta actaccacag acatttaggt attaacatgg ggaccgaagt tcattacaaa ctaaaatatc ccaaaaaagt atgttaccat ttaattaata aacattttaa agagataaaa aagaaaaaat ataaaagccg tgttgattca tattttaata agaattgcat tgaaaatagt agtgtaaaaa atagggagtg tttttataat atatctaata ataaagaaga aaaaagaaac agccgtaaat ccatagaaaa gttacaaatc agaaaataca ttgagaaatg caattttaaa tcaaatatat tctctcttat tttaaatttg ggattaaaaa aagatgctac aattgaagta tgtaaaatga taaaaaaggc agaaaattta attgaaaaaa acatttttga aaaattaaac gttgtcaagg taatagaagt aaataagctt aaaaacaagc aacaaaaatt gaaaagaata ctaaaagaag taaaaattaa attagaaaac gaaaattata aaagcgagca attagaaata gaaataaaag atatatatga gcaatataag aataaaccac attttatcat agaaaaaaat aagtacgaag atttagagaa gataacagaa aaacttaaaa ataagtttaa acaagaaaaa ataaatataa aagaaaatga cacaaatatt agaaataata tctttaatat acttattgaa caattaagac ataaaataaa gattgaaatt ttaataccga ttttaaaaga ttatttggat aagcaaaaaa gattagaata tagcaaggtc tttagcaatt attactatta cgagatctta gagacagtag agcctaataa atggtgttca caattagagg catgtaaata a

Details for BobuA.18548.a.B1.GE38705

HARVESTED ON:	12/9/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	46kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMK SIKTSTNKYQ HKLIILISTL NYVNSNLEQY TQNDILYYFN NNIRRNCQKE VKLKTLQNYL YKLEKVFKVT SNYHRHLGIN MGTEVHYKLK YPKKVCYHLI NKHFKEIKKK KYKSRVDSYF NKNCIENSSV KNRECFYNIS NNKEEKRNSR KSIEKLQIRK YIEKCNFKSN IFSLILNLGL KKDATIEVCK MIKKAENLIE KNIFEKLNVV KVIEVNKLKN KQQKLKRILK EVKIKLENEN YKSEQLEIEI KDIYEQYKNK PHFIIEKNKY EDLEKITEKL KNKFKQEKIN IKENDTNIRN NIFNILIEQL RHKIKIEILI PILKDYLDKQ KRLEYSKVFS NYYYYEILET VEPNKW

Validated NT Sequence

acaccattta ttaggctcta ctgtctctaa gatctcgtaa tagtaataat tgctaaagac cttgctatat tctaatcttt tttgcttatc caaataatct tttaaaatcg gtattaaaat ttcaatcttt attttatgtc ttaattgttc aataagtata ttaaagatat tatttctaat atttgtgtca ttttctttta tatttatttt ttcttgttta aacttatttt taagtttttc tgttatcttc tctaaatctt cgtacttatt tttttctatg ataaaatgtg gtttattctt atattgctca tatatatctt ttatttctat ttctaattgc tcgcttttat aattttcgtt ttctaattta atttttactt cttttagtat tcttttcaat ttttgttgct tgtttttaag cttatttact tctattacct tgacaacgtt taatttttca aaaatgtttt tttcaattaa attttctgcc ttttttatca ttttacatac ttcaattgta gcatcttttt ttaatcccaa atttaaaata agagagaata tatttgattt aaaattgcat ttctcaatgt attttctgat ttgtaacttt tctatggatt tacggctgtt tcttttttct tctttattat tagatatatt ataaaaacac tccctatttt ttacactact attttcaatg caattcttat taaaatatga atcaacacgg cttttatatt ttttcttttt tatctcttta aaatgtttat taattaaatg gtaacatact tttttgggat attttagttt gtaatgaact tcggtcccca tgttaatacc taaatgtctg tggtagttac tagttacttt aaataccttt tctaatttgt aaagatagtt ttgtagtgtt tttagcttaa cttctttttg acaatttctt cttatattat tattaaagta ataaagtatg tcattttgag tgtattgttc aagatttgaa tttacataat tcagtgttga tattagaatg attaatttgt gttggtattt atttgtagag gtttttatac ttttcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa gtataaaaac ctctacaaat aaataccaac acaaattaat cattctaata tcaacactga attatgtaaa ttcaaatctt gaacaataca ctcaaaatga catactttat tactttaata ataatataag aagaaattgt caaaaagaag ttaagctaaa aacactacaa aactatcttt acaaattaga aaaggtattt aaagtaacta gtaactacca cagacattta ggtattaaca tggggaccga agttcattac aaactaaaat atcccaaaaa agtatgttac catttaatta ataaacattt taaagagata aaaaagaaaa aatataaaag ccgtgttgat tcatatttta ataagaattg cattgaaaat agtagtgtaa aaaataggga gtgtttttat aatatatcta ataataaaga agaaaaaaga aacagccgta aatccataga aaagttacaa atcagaaaat acattgagaa atgcaatttt aaatcaaata tattctctct tattttaaat ttgggattaa aaaaagatgc tacaattgaa gtatgtaaaa tgataaaaaa ggcagaaaat ttaattgaaa aaaacatttt tgaaaaatta aacgttgtca aggtaataga agtaaataag cttaaaaaca agcaacaaaa attgaaaaga atactaaaag aagtaaaaat taaattagaa aacgaaaatt ataaaagcga gcaattagaa atagaaataa aagatatata tgagcaatat aagaataaac cacattttat catagaaaaa aataagtacg aagatttaga gaagataaca gaaaaactta aaaataagtt taaacaagaa aaaataaata taaaagaaaa tgacacaaat attagaaata atatctttaa tatacttatt gaacaattaa gacataaaat aaagattgaa attttaatac cgattttaaa agattatttg gataagcaaa aaagattaga atatagcaag gtctttagca attattacta ttacgagatc ttagagacag tagagcctaa taaatggtgt tcacaattag aggcatgtaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat