BobuA.18357.a

Probable M18 family aminopeptidase 2 (EC 3.4.11.-) (BB_0627)

CENTER ID: BobuA.18357.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18357.a.B1.GE38701 full length 1 423
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1017
RefSeq: YP_008686584.1
UniProt: O51572

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVXXKTLEPK FFQSLLDNSP TPYHLVNYIE EKLINYFNAQ QLKLNEKWKI KTGSYYIKKE GTSLIAFNID VKKKYEPFLI AAAHTDSPGL KLKIDATEKV SGVFYNHIEV YGSPIISTWI DRDLSLAGIV YFKKNENIES KLINIENIGI IPNLAIHLNR QINEGFKYNA HDNLTVISST KKAIKDNILE QLGIECENFL SCDLIFTESQ PSKIIGTEGE FLASKNLDNK SGCHAIMNSY VHTSNDKNKI AVFFDNEEVG SLTSRGADSN FLSEVLERID IALDLTREEH LIKTNKSFNI SIDSVHGIHP GYTSKHDPNY QANLGKGVVV KNSANFRYAT TSTGFAKLKN LAIKNNIKIQ EIIMKANVPS GTTIGPISNA RTGIETIDIG TPMWAMHSLR ETVSIADHIE AIKLLRAFFE KGI
NT Sequence
atggtgamtg mtaaaacact agaaccaaaa ttttttcaaa gtctgctaga caatagccct accccttatc acttagtaaa ctatattgaa gagaaattaa taaattattt taatgcacaa caattaaaac ttaatgaaaa atggaaaatt aaaacaggat catattacat aaaaaaagaa ggaactagcc ttattgcctt taatattgat gtcaaaaaaa aatatgaacc gtttctaata gcagcagcac atacagacag tccgggatta aaattaaaaa tagacgcaac agaaaaagta agtggtgtgt tttataacca tattgaagtt tatggtagtc caataatttc tacttggatt gacagagact taagcttagc aggaattgta tatttcaaaa aaaatgagaa tattgaatca aaattaatca acattgaaaa cataggaatt attccaaacc ttgcaatcca tttaaaccga caaattaacg aaggatttaa atacaatgct catgacaatt taacagtaat cagtagcact aaaaaagcaa taaaagataa tatcttagaa caacttggaa tagagtgtga aaattttcta tcttgtgatt taatattcac agaatcacaa ccttctaaaa taataggaac tgaaggagaa tttttggctt ctaaaaatct tgacaacaaa tcgggatgcc atgcaatcat gaattcttat gttcatacaa gcaatgataa aaataaaata gctgtatttt ttgataacga agaagtaggt tctttaacat caagaggcgc tgattcaaat tttttatcag aagttttaga aagaatcgat attgctcttg atttaaccag agaagagcat ttaataaaaa caaacaaatc atttaatatc tcaattgaca gcgttcacgg cattcatccg ggttatacat ctaaacatga tccaaactat caagcaaatc taggtaaggg cgtagttgta aaaaatagtg ctaatttcag atatgcaaca acttcaacag gatttgcaaa attaaaaaat ttggctatta aaaacaatat taagattcaa gaaataataa tgaaagcaaa tgttccttca ggcacaacaa ttggtccaat ctcaaatgca agaacaggaa tagaaactat tgacattgga acaccaatgt gggcaatgca ttccctgcgc gaaacagtat caatagctga ccacatagaa gcaattaaat tgctaagggc tttctttgaa aaaggaattt aa
Details for BobuA.18357.a.B1.GE38701
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV TAKTLEPKFF QSLLDNSPTP YHLVNYIEEK LINYFNAQQL KLNEKWKIKT GSYYIKKEGT SLIAFNIDVK KKYEPFLIAA AHTDSPGLKL KIDATEKVSG VFYNHIEVYG SPIISTWIDR DLSLAGIVYF KKNENIESKL INIENIGIIP NLAIHLNRQI NEGFKYNAHD NLTVISSTKK AIKDNILEQL GIECENFLSC DLIFTESQPS KIIGTEGEFL ASKNLDNKSG CHAIMNSYVH TSNDKNKIAV FFDNEEVGSL TSRGADSNFL SEVLERIDIA LDLTREEHLI KTNKSFNISI DSVHGIHPGY TSKHDPNYQA NLGKGVVVKN SANFRYATTS TGFAKLKNLA IKNNIKIQEI IMKANVPSGT TIGPISNART GIETIDIGTP MWAMHSLRET VSIADHIEAI KLL
Validated NT Sequence
ccttagcaat ttaattgctt ctatgtggtc agctattgat actgtttcgc gcagggaatg cattgcccac attggtgttc caatgtcaat agtttctatt cctgttcttg catttgagat tggaccaatt gttgtgcctg aaggaacatt tgctttcatt attatttctt gaatcttaat attgttttta atagccaaat tttttaattt tgcaaatcct gttgaagttg ttgcatatct gaaattagca ctatttttta caactacgcc cttacctaga tttgcttgat agtttggatc atgtttagat gtataacccg gatgaatgcc gtgaacgctg tcaattgaga tattaaatga tttgtttgtt tttattaaat gctcttctct ggttaaatca agagcaatat cgattctttc taaaacttct gataaaaaat ttgaatcagc gcctcttgat gttaaagaac ctacttcttc gttatcaaaa aatacagcta ttttattttt atcattgctt gtatgaacat aagaattcat gattgcatgg catcccgatt tgttgtcaag atttttagaa gccaaaaatt ctccttcagt tcctattatt ttagaaggtt gtgattctgt gaatattaaa tcacaagata gaaaattttc acactctatt ccaagttgtt ctaagatatt atcttttatt gcttttttag tgctactgat tactgttaaa ttgtcatgag cattgtattt aaatccttcg ttaatttgtc ggtttaaatg gattgcaagg tttggaataa ttcctatgtt ttcaatgttg attaattttg attcaatatt ctcatttttt ttgaaatata caattcctgc taagcttaag tctctgtcaa tccaagtaga aattattgga ctaccataaa cttcaatatg gttataaaac acaccactta ctttttctgt tgcgtctatt tttaatttta atcccggact gtctgtatgt gctgctgcta ttagaaacgg ttcatatttt tttttgacat caatattaaa ggcaataagg ctagttcctt ctttttttat gtaatatgat cctgttttaa ttttccattt ttcattaagt tttaattgtt gtgcattaaa ataatttatt aatttctctt caatatagtt tactaagtga taaggggtag ggctattgtc tagcagactt tgaaaaaatt ttggttctag tgttttagca gtcaccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMV XXKTLEPKFF QSLLDNSPTP YHLVNYIEEK LINYFNAQQL KLNEKWKIKT GSYYIKKEGT SLIAFNIDVK KKYEPFLIAA AHTDSPGLKL KIDATEKVSG VFYNHIEVYG SPIISTWIDR DLSLAGIVYF KKNENIESKL INIENIGIIP NLAIHLNRQI NEGFKYNAHD NLTVISSTKK AIKDNILEQL GIECENFLSC DLIFTESQPS KIIGTEGEFL ASKNLDNKSG CHAIMNSYVH TSNDKNKIAV FFDNEEVGSL TSRGADSNFL SEVLERIDIA LDLTREEHLI KTNKSFNISI DSVHGIHPGY TSKHDPNYQA NLGKGVVVKN SANFRYATTS TGFAKLKNLA IKNNIKIQEI IMKANVPSGT TIGPISNART GIETIDIGTP MWAMHSLRET VSIADHIEAI KLLRAFFEKG I
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga mtgmtaaaac actagaacca aaattttttc aaagtctgct agacaatagc cctacccctt atcacttagt aaactatatt gaagagaaat taataaatta ttttaatgca caacaattaa aacttaatga aaaatggaaa attaaaacag gatcatatta cataaaaaaa gaaggaacta gccttattgc ctttaatatt gatgtcaaaa aaaaatatga accgtttcta atagcagcag cacatacaga cagtccggga ttaaaattaa aaatagacgc aacagaaaaa gtaagtggtg tgttttataa ccatattgaa gtttatggta gtccaataat ttctacttgg attgacagag acttaagctt agcaggaatt gtatatttca aaaaaaatga gaatattgaa tcaaaattaa tcaacattga aaacatagga attattccaa accttgcaat ccatttaaac cgacaaatta acgaaggatt taaatacaat gctcatgaca atttaacagt aatcagtagc actaaaaaag caataaaaga taatatctta gaacaacttg gaatagagtg tgaaaatttt ctatcttgtg atttaatatt cacagaatca caaccttcta aaataatagg aactgaagga gaatttttgg cttctaaaaa tcttgacaac aaatcgggat gccatgcaat catgaattct tatgttcata caagcaatga taaaaataaa atagctgtat tttttgataa cgaagaagta ggttctttaa catcaagagg cgctgattca aattttttat cagaagtttt agaaagaatc gatattgctc ttgatttaac cagagaagag catttaataa aaacaaacaa atcatttaat atctcaattg acagcgttca cggcattcat ccgggttata catctaaaca tgatccaaac tatcaagcaa atctaggtaa gggcgtagtt gtaaaaaata gtgctaattt cagatatgca acaacttcaa caggatttgc aaaattaaaa aatttggcta ttaaaaacaa tattaagatt caagaaataa taatgaaagc aaatgttcct tcaggcacaa caattggtcc aatctcaaat gcaagaacag gaatagaaac tattgacatt ggaacaccaa tgtgggcaat gcattccctg cgcgaaacag tatcaatagc tgaccacata gaagcaatta aattgctaag ggctttcttt gaaaaaggaa tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat