BobuA.18305.a

ribonuclease III (rnc) (BB_0705)

CENTER ID: BobuA.18305.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18305.a.B1.GE39294 full length 1 245
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18305.a.B1.PW37784 full length 1 245

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1096
RefSeq: NP_212839.2
UniProt: O51648

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKKSSDFCL CNERKSQLSK FLENLSIDFS NFDLLNTALC HSSYSNELDQ KSSNNERLEF LGDSVLNLII TDHLYKTYPN KSEGELSKAR SYIVSEDSLS NIAREINLGS YILLGRGEES NDGRNKKGIL ADAIEAFVGA IYLDSGFSRA TEFVVGLFDM YIRLMFNRGD FKDYKSLLQE YVQKKYKISP SYKLDKEIGP DHDKVFCVEL YVGENFISNG KGKSKKEAEM RAAEVALKAM ENINL
NT Sequence
atgaaaaaaa aatcttctga tttttgtttg tgtaatgaaa gaaaatctca attgagtaaa tttttggaaa atttgagcat tgactttagt aattttgatt tattgaatac agcattgtgt cattcgtcgt attctaatga gttggatcaa aaatctagta ataatgagag attagaattt ttgggagatt ctgtgcttaa tttgattatt acagatcatc tttataaaac ttatccaaat aaaagtgaag gagagctcag taaggccaga tcttatattg ttagtgaaga ttccctatct aatattgcta gagagattaa tcttggttct tatattttgc taggtagagg ggaggagagt aatgatggtc gaaataaaaa aggcattctt gcagatgcta ttgaagcttt tgtaggcgct atttatcttg atagtgggtt ttcaagagca acagaatttg tggttggact ttttgatatg tatataagat tgatgtttaa taggggggat tttaaagatt ataagagttt gttgcaagaa tatgttcaaa agaaatataa aatctcgcca agttataagc tagacaagga aataggtcca gaccatgata aagttttttg tgtggaactt tatgttggag aaaattttat atcaaatgga aagggcaaat ctaaaaaaga agccgaaatg agagcggctg aagtagcttt aaaagctatg gaaaacatta acctttaa
Details for BobuA.18305.a.B1.GE39294
HARVESTED ON: 7/10/2015
SEQUENCED ON: 7/6/2015
EXPECTED MW: 29kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMK KKSSDFCLCN ERKSQLSKFL ENLSIDFSNF DLLNTALCHS SYSNELDQKS SNNERLEFLG DSVLNLIITD HLYKTYPNKS EGELSKARSY IVSEDSLSNI AREINLGSYI LLGRGEECND GRNKKGILAD AIEAFVGAIY LDSGFSRATE FVVGLFDMYI RLMFNRGDFK DYKSLLQEYV QKKYKISPS
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa aaaaaatctt ctgatttttg tttgtgtaat gaaagaaaat ctcaattgag taaatttttg gaaaatttga gcattgactt tagtaatttt gatttattga atacagcatt gtgtcattcg tcgtattcta atgagttgga tcaaaaatct agtaataatg agagattaga atttttggga gattctgtgc ttaatttgat tattacagat catctttata aaacttatcc aaataaaagt gaaggagagc tcagtaaggc cagatcttat attgttagtg aagattccct atctaatatt gctagagaga ttaatcttgg ttcttatatt ttgctaggta gaggggagga gtgtaatgat ggtcgaaata aaaaaggcat tcttgcagat gctattgaag cttttgtagg cgctatttat cttgatagtg ggttttcaag agcaacagaa tttgtggttg gactttttga tatgtatata agattgatgt ttaatagggg ggattttaaa gattataaga gtttgttgca agaatatgtt caaaagaaat ataaaatctc gccaagttaa gctagacaag gaaataggtc cagaccatga taaagttttt tgtgtggaac tttatgttgg agaaaatttt atatcaaatg gaaagggcaa atctaaaaaa gaagccgaaa tgagagcggc tgaagtagct ttaaaagcta tggaaaacat taacctttaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMK KKSSDFCLCN ERKSQLSKFL ENLSIDFSNF DLLNTALCHS SYSNELDQKS SNNERLEFLG DSVLNLIITD HLYKTYPNKS EGELSKARSY IVSEDSLSNI AREINLGSYI LLGRGEESND GRNKKGILAD AIEAFVGAIY LDSGFSRATE FVVGLFDMYI RLMFNRGDFK DYKSLLQEYV QKKYKISPSY KLDKEIGPDH DKVFCVELYV GENFISNGKG KSKKEAEMRA AEVALKAMEN INL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aaaaatcttc tgatttttgt ttgtgtaatg aaagaaaatc tcaattgagt aaatttttgg aaaatttgag cattgacttt agtaattttg atttattgaa tacagcattg tgtcattcgt cgtattctaa tgagttggat caaaaatcta gtaataatga gagattagaa tttttgggag attctgtgct taatttgatt attacagatc atctttataa aacttatcca aataaaagtg aaggagagct cagtaaggcc agatcttata ttgttagtga agattcccta tctaatattg ctagagagat taatcttggt tcttatattt tgctaggtag aggggaggag agtaatgatg gtcgaaataa aaaaggcatt cttgcagatg ctattgaagc ttttgtaggc gctatttatc ttgatagtgg gttttcaaga gcaacagaat ttgtggttgg actttttgat atgtatataa gattgatgtt taataggggg gattttaaag attataagag tttgttgcaa gaatatgttc aaaagaaata taaaatctcg ccaagttata agctagacaa ggaaataggt ccagaccatg ataaagtttt ttgtgtggaa ctttatgttg gagaaaattt tatatcaaat ggaaagggca aatctaaaaa agaagccgaa atgagagcgg ctgaagtagc tttaaaagct atggaaaaca ttaacctttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BobuA.18305.a.B1.PW37784
PURIFICATION DATe: 9/16/2015
CONCENTRATION: 5mg/ml
OBSERVED MW: 25kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 32
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 78
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK KKSSDFCLCN ERKSQLSKFL ENLSIDFSNF DLLNTALCHS SYSNELDQKS SNNERLEFLG DSVLNLIITD HLYKTYPNKS EGELSKARSY IVSEDSLSNI AREINLGSYI LLGRGEECND GRNKKGILAD AIEAFVGAIY LDSGFSRATE FVVGLFDMYI RLMFNRGDFK DYKSLLQEYV QKKYKISPS
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa aaaaaatctt ctgatttttg tttgtgtaat gaaagaaaat ctcaattgag taaatttttg gaaaatttga gcattgactt tagtaatttt gatttattga atacagcatt gtgtcattcg tcgtattcta atgagttgga tcaaaaatct agtaataatg agagattaga atttttggga gattctgtgc ttaatttgat tattacagat catctttata aaacttatcc aaataaaagt gaaggagagc tcagtaaggc cagatcttat attgttagtg aagattccct atctaatatt gctagagaga ttaatcttgg ttcttatatt ttgctaggta gaggggagga gtgtaatgat ggtcgaaata aaaaaggcat tcttgcagat gctattgaag cttttgtagg cgctatttat cttgatagtg ggttttcaag agcaacagaa tttgtggttg gactttttga tatgtatata agattgatgt ttaatagggg ggattttaaa gattataaga gtttgttgca agaatatgtt caaaagaaat ataaaatctc gccaagttaa gctagacaag gaaataggtc cagaccatga taaagttttt tgtgtggaac tttatgttgg agaaaatttt atatcaaatg gaaagggcaa atctaaaaaa gaagccgaaa tgagagcggc tgaagtagct ttaaaagcta tggaaaacat taacctttaa gtgagtaaga t
Expressed Protein Sequence
MAHHHHHHMK KKSSDFCLCN ERKSQLSKFL ENLSIDFSNF DLLNTALCHS SYSNELDQKS SNNERLEFLG DSVLNLIITD HLYKTYPNKS EGELSKARSY IVSEDSLSNI AREINLGSYI LLGRGEESND GRNKKGILAD AIEAFVGAIY LDSGFSRATE FVVGLFDMYI RLMFNRGDFK DYKSLLQEYV QKKYKISPSY KLDKEIGPDH DKVFCVELYV GENFISNGKG KSKKEAEMRA AEVALKAMEN INL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA AAAAATCTTC TGATTTTTGT TTGTGTAATG AAAGAAAATC TCAATTGAGT AAATTTTTGG AAAATTTGAG CATTGACTTT AGTAATTTTG ATTTATTGAA TACAGCATTG TGTCATTCGT CGTATTCTAA TGAGTTGGAT CAAAAATCTA GTAATAATGA GAGATTAGAA TTTTTGGGAG ATTCTGTGCT TAATTTGATT ATTACAGATC ATCTTTATAA AACTTATCCA AATAAAAGTG AAGGAGAGCT CAGTAAGGCC AGATCTTATA TTGTTAGTGA AGATTCCCTA TCTAATATTG CTAGAGAGAT TAATCTTGGT TCTTATATTT TGCTAGGTAG AGGGGAGGAG AGTAATGATG GTCGAAATAA AAAAGGCATT CTTGCAGATG CTATTGAAGC TTTTGTAGGC GCTATTTATC TTGATAGTGG GTTTTCAAGA GCAACAGAAT TTGTGGTTGG ACTTTTTGAT ATGTATATAA GATTGATGTT TAATAGGGGG GATTTTAAAG ATTATAAGAG TTTGTTGCAA GAATATGTTC AAAAGAAATA TAAAATCTCG CCAAGTTATA AGCTAGACAA GGAAATAGGT CCAGACCATG ATAAAGTTTT TTGTGTGGAA CTTTATGTTG GAGAAAATTT TATATCAAAT GGAAAGGGCA AATCTAAAAA AGAAGCCGAA ATGAGAGCGG CTGAAGTAGC TTTAAAAGCT ATGGAAAACA TTAACCTTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT