SSGCID: Target Page

BobuA.18263.a

mannose-6-isomerase (manA) (BB_0407)

CENTER ID:	BobuA.18263.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18263.a.B1.GE39293	full length		1	372

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18263.a.B1.PW37782	full length		1	372

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.801
RefSeq:	NP_212541.1
UniProt:	O51368

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNEDNIFLM KNNIKEYDWG GINFIPNLLG DKIDGKPKAE MWLGAHKTFS SKILYKNEYV LLSDFLEDHK ELLGCNDEFP FLLKVLSANK PLSIQIHPSK DIALKGYESE NNKGIDINDP KRTYKDKNPK IELIYALSDF YALKGFLPLD EIKKIYEILE LNFDFQSHKD FVKTIFDLQM YELEKIIEKI LKNLDLIDNF RGYWFNEIYN IYGIDVGLLV FLGMNILKLK PGEVVYTNSQ EVHAYLKGDC IELMTNSDNV IRAGLTTKYI DKDEMLRVGQ FEEGKLSFLN PDFQDNFSVF RLPNTNLKLI QKKINENICI NRNSAMVLLV LNGCVSINKS LNLKKGESIF IGKKAENLFI DGDGEAFIAG FN

NT Sequence

atgaataatg aagataatat ttttttaatg aaaaataata ttaaagaata tgattggggc ggaattaatt ttattcccaa tcttttgggt gataagattg atggaaagcc caaggctgaa atgtggcttg gagcacacaa gacattttct agtaagattt tgtataaaaa tgaatatgtg cttttaagcg attttttaga agatcataaa gagcttttag gctgtaatga cgaatttcct tttttgctta aggtattgtc tgcaaataag cccctgtcta ttcaaattca tccttctaaa gatattgcct taaaagggta tgaatcagag aataataaag ggatagacat taatgatccc aaaaggacat acaaagacaa aaaccccaaa attgaactta tttatgctct tagtgatttt tatgctctta aaggcttttt acccttagat gagattaaaa aaatttatga aattctggaa ttaaatttcg actttcaatc acataaagat tttgtaaaga ctatttttga tttacaaatg tatgaacttg agaagattat tgaaaaaatt ttaaaaaatt tggatcttat tgataatttt aggggctatt ggtttaatga aatttacaat atttatggta tagatgtggg ccttttggta tttttaggta tgaatatttt aaaactaaaa ccaggagaag ttgtttatac aaatagtcag gaggtgcatg catatcttaa gggagattgc attgagctta tgaccaattc cgacaatgtt attagggctg ggcttactac aaagtatatt gataaagacg agatgttaag agttggtcaa tttgaggaag gaaagttatc atttttaaat cccgattttc aagataattt tagcgtattt agacttccaa atactaattt gaaattgatt caaaaaaaaa taaatgagaa catttgtatt aatagaaata gtgcaatggt cttgctagtt ttaaatgggt gcgtgagtat aaataaatcc ttaaatctta agaaaggtga gagcatattt ataggtaaaa aagcagaaaa cttgtttatt gatggggacg gcgaagcttt tattgctggt tttaattaa

Details for BobuA.18263.a.B1.GE39293

HARVESTED ON:	7/10/2015
SEQUENCED ON:	7/6/2015
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMN NEDNIFLMKN NIKEYDWGGI NFIPNLLGDK IDGKPKAEMW LGAHKTFSSK ILYKNEYVLL SDFLEDHKEL LGCNDEFPFL LKVLSANKPL SIQIHPSKDI ALKGYESENN KGIDINDPKR TYKDKNPKIE LIYALSDFYA LKGFLPLDEI KKIYEILELN FDFQSHKDFV KTIFDLQMYE LEKIIEKILK NLDLIDNFRG YWFNEIYNIY GIDVGLLVFL GMNILKLKPG EVVYTNSQEV HAYLKGDCIE LMTNSDNVIR AGLTTKYIDK DEMLRVGQFE EGKLSFLNPD FQDNFSVFRL PNTNLKLIQK KINENICINR NSAMVLLVLN GCVSINKSLN LKKGESIFIG KKAENLFIDG D

Validated NT Sequence

ccgtccccat caataaacaa gttttctgct tttttaccta taaatatgct ctcacctttc ttaagattta aggatttatt tatactcacg cacccattta aaactagcaa gaccattgca ctatttctat taatacaaat gttctcattt attttttttt gaatcaattt caaattagta tttggaagtc taaatacgct aaaattatct tgaaaatcgg gatttaaaaa tgataacttt ccttcctcaa attgaccaac tcttaacatc tcgtctttat caatatactt tgtagtaagc ccagccctaa taacattgtc ggaattggtc ataagctcaa tgcaatctcc cttaagatat gcatgcacct cctgactatt tgtataaaca acttctcctg gttttagttt taaaatattc atacctaaaa ataccaaaag gcccacatct ataccataaa tattgtaaat ttcattaaac caatagcccc taaaattatc aataagatcc aaatttttta aaattttttc aataatcttc tcaagttcat acatttgtaa atcaaaaata gtctttacaa aatctttatg tgattgaaag tcgaaattta attccagaat ttcataaatt tttttaatct catctaaggg taaaaagcct ttaagagcat aaaaatcact aagagcataa ataagttcaa ttttggggtt tttgtctttg tatgtccttt tgggatcatt aatgtctatc cctttattat tctctgattc ataccctttt aaggcaatat ctttagaagg atgaatttga atagacaggg gcttatttgc agacaatacc ttaagcaaaa aaggaaattc gtcattacag cctaaaagct ctttatgatc ttctaaaaaa tcgcttaaaa gcacatattc atttttatac aaaatcttac tagaaaatgt cttgtgtgct ccaagccaca tttcagcctt gggctttcca tcaatcttat cacccaaaag attgggaata aaattaattc cgccccaatc atattcttta atattatttt tcattaaaaa aatattatct tcattattca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata atgaagataa tattttttta atgaaaaata atattaaaga atatgattgg ggcggaatta attttattcc caatcttttg ggtgataaga ttgatggaaa gcccaaggct gaaatgtggc ttggagcaca caagacattt tctagtaaga ttttgtataa aaatgaatat gtgcttttaa gcgatttttt agaagatcat aaagagcttt taggctgtaa tgacgaattt ccttttttgc ttaaggtatt gtctgcaaat aagcccctgt ctattcaaat tcatccttct aaagatattg ccttaaaagg gtatgaatca gagaataata aagggataga cattaatgat cccaaaagga catacaaaga caaaaacccc aaaattgaac ttatttatgc tcttagtgat ttttatgctc ttaaaggctt tttaccctta gatgagatta aaaaaattta tgaaattctg gaattaaatt tcgactttca atcacataaa gattttgtaa agactatttt tgatttacaa atgtatgaac ttgagaagat tattgaaaaa attttaaaaa atttggatct tattgataat tttaggggct attggtttaa tgaaatttac aatatttatg gtatagatgt gggccttttg gtatttttag gtatgaatat tttaaaacta aaaccaggag aagttgttta tacaaatagt caggaggtgc atgcatatct taagggagat tgcattgagc ttatgaccaa ttccgacaat gttattaggg ctgggcttac tacaaagtat attgataaag acgagatgtt aagagttggt caatttgagg aaggaaagtt atcattttta aatcccgatt ttcaagataa ttttagcgta tttagacttc caaatactaa tttgaaattg attcaaaaaa aaataaatga gaacatttgt attaatagaa atagtgcaat ggtcttgcta gttttaaatg ggtgcgtgag tataaataaa tccttaaatc ttaagaaagg tgagagcata tttataggta aaaaagcaga aaacttgttt attgatgggg acggcgaagc ttttattgct ggttttaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BobuA.18263.a.B1.PW37782

PURIFICATION DATe:	9/8/2015
CONCENTRATION:	33mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA ATGAAGATAA TATTTTTTTA ATGAAAAATA ATATTAAAGA ATATGATTGG GGCGGAATTA ATTTTATTCC CAATCTTTTG GGTGATAAGA TTGATGGAAA GCCCAAGGCT GAAATGTGGC TTGGAGCACA CAAGACATTT TCTAGTAAGA TTTTGTATAA AAATGAATAT GTGCTTTTAA GCGATTTTTT AGAAGATCAT AAAGAGCTTT TAGGCTGTAA TGACGAATTT CCTTTTTTGC TTAAGGTATT GTCTGCAAAT AAGCCCCTGT CTATTCAAAT TCATCCTTCT AAAGATATTG CCTTAAAAGG GTATGAATCA GAGAATAATA AAGGGATAGA CATTAATGAT CCCAAAAGGA CATACAAAGA CAAAAACCCC AAAATTGAAC TTATTTATGC TCTTAGTGAT TTTTATGCTC TTAAAGGCTT TTTACCCTTA GATGAGATTA AAAAAATTTA TGAAATTCTG GAATTAAATT TCGACTTTCA ATCACATAAA GATTTTGTAA AGACTATTTT TGATTTACAA ATGTATGAAC TTGAGAAGAT TATTGAAAAA ATTTTAAAAA ATTTGGATCT TATTGATAAT TTTAGGGGCT ATTGGTTTAA TGAAATTTAC AATATTTATG GTATAGATGT GGGCCTTTTG GTATTTTTAG GTATGAATAT TTTAAAACTA AAACCAGGAG AAGTTGTTTA TACAAATAGT CAGGAGGTGC ATGCATATCT TAAGGGAGAT TGCATTGAGC TTATGACCAA TTCCGACAAT GTTATTAGGG CTGGGCTTAC TACAAAGTAT ATTGATAAAG ACGAGATGTT AAGAGTTGGT CAATTTGAGG AAGGAAAGTT ATCATTTTTA AATCCCGATT TTCAAGATAA TTTTAGCGTA TTTAGACTTC CAAATACTAA TTTGAAATTG ATTCAAAAAA AAATAAATGA GAACATTTGT ATTAATAGAA ATAGTGCAAT GGTCTTGCTA GTTTTAAATG GGTGCGTGAG TATAAATAAA TCCTTAAATC TTAAGAAAGG TGAGAGCATA TTTATAGGTA AAAAAGCAGA AAACTTGTTT ATTGATGGGG ACGGCGAAGC TTTTATTGCT GGTTTTAATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT