SSGCID: Target Page

BobuA.18057.a

Arginine deiminase (ADI) (EC 3.5.3.6) (Arginine dihydrolase) (AD) (BB_0841)

CENTER ID:	BobuA.18057.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18057.a.B1.GE39228	full length		1	410

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.1234
RefSeq:	NP_212975.1
UniProt:	O51781

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEEEYLNPIN IFSEIGRLKK VLLHRPGEEL ENLTPLIMKN FLFDDIPYLK VARQEHEVFV NILKDNSVEI EYVEDLVSEV LASSVALKNK FISQFILEAE IKTDGVINIL KDYFSNLTVD NMVSKMISGV AREELKDCEF SLDDWVNGSS LFVIDPMPNV LFTRDPFASI GNGITINKMY TKVRRRETIF AEYIFKYHSA YKENVPIWFN RWEETSLEGG DEFVLNKDLL VIGISERTEA GSVEKLAASL FKNKAPFSTI LAFKIPKNRA YMHLDTVFTQ IDYSVFTSFT SDDMYFSIYV LTYNSNSNKI NIKKEKAKLK DVLSFYLGRK IDIIKCAGGD LIHGAREQWN DGANVLAIAP GEVIAYSRNH VTNKLFEENG IKVHRIPSSE LSRGRGGPRC MSMSLVREDI

NT Sequence

atggaagaag aatatttaaa tccaataaat atattttcgg aaataggtcg tttgaaaaaa gttttgcttc ataggccagg agaagaatta gaaaatttga cacccttgat tatgaaaaat tttttatttg atgatattcc ttatcttaaa gttgcaagac aagagcatga agtttttgta aatattttaa aagataattc agttgaaatt gagtatgttg aggatcttgt tagtgaagtt cttgcttctt ctgtagcgct caaaaataaa tttatatctc aatttattct ggaagcagaa ataaaaacag atggtgtaat taatatttta aaagattatt tttctaattt aaccgttgat aatatggttt ctaaaatgat ttccggcgtt gcaagagaag agcttaaaga ttgtgaattt tcgcttgatg attgggttaa tggttcaagt ctttttgtta ttgatcctat gcccaatgtt ttatttacca gagatccttt tgccagtatt ggcaatggaa ttacaataaa taaaatgtat accaaggtta gacgtagaga gacaatattt gcagagtata tttttaaata tcattccgct tacaaagaaa atgttccaat ttggtttaat agatgggaag aaacttcttt ggaaggtggg gatgagtttg ttttaaataa agatcttttg gttattggaa tctcagaaag aacagaagcg gggtctgtag aaaaactagc tgctagtctt tttaaaaata aggctccatt tagcacaatt ttggctttta aaattccaaa aaacagagcc tatatgcact tagacacagt ttttacccaa attgattata gcgtttttac aagttttaca agtgatgata tgtatttctc aatttatgtt ttaacttaca attcaaattc taataaaatt aatattaaaa aagaaaaagc caagcttaaa gatgttttga gcttttattt aggcagaaaa attgacataa taaaatgtgc gggtggagat ttaatacatg gtgcaagaga acaatggaat gatggtgcta atgttttggc gatagctcca ggagaagtaa ttgcttattc tagaaatcat gtaactaata agctgtttga agaaaatggt attaaagttc acagaattcc gtctagcgag ctttcaaggg gtcgtggtgg gccaagatgc atgtctatgt ctttagtaag agaggatatt taa

Details for BobuA.18057.a.B1.GE39228

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/5/2015
EXPECTED MW:	48kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	78

Validated AA Sequence

MAHHHHHHME EEYLNPINIF SEIGRLKKVL LHRPGEELEN LTPLIMKNFL FDDIPYLKVA RQEHEVFVNI LKDNSVEIEY VEDLVSEVLA SSVALKNKFI SQFILEAEIK TDGVINILKD YFSNLTVDNM VSKMISGVAR EELKDCEFSL DDWVNGSSLF VIDPMPNVLF TRDPFASIGN GITINKMYTK VRRRETIFAE YIFKYHSAYK ENVPIWFNRW EETSLEGGDE FVLNKDLLVI GISERTEAGS VEKLAASLFK NKAPFSTILA FKIPKNRAYM HLDTVFTQID YSVFTSFTSD DMYFSIYVLT YNSNSNKINI KKEKASLKMF

Validated NT Sequence

atagacatgc atcttggccc accacgaccc cttgaaagct cgctagacgg aattctgtga actttaatac cattttcttc aaacagctta ttagttacat gatttctaga ataagcaatt acttctcctg gagctatcgc caaaacatta gcaccatcat tccattgttc tcttgcacca tgtattaaat ctccacccgc acattttatt atgtcaattt ttctgcctaa ataaaagctc aaaacatctt taagcttgct ttttcttttt taatattaat tttattagaa tttgaattgt aagttaaaac ataaattgag aaatacatat catcacttgt aaaacttgta aaaacgctat aatcaatttg ggtaaaaact gtgtctaagt gcatataggc tctgtttttt ggaattttaa aagccaaaat tgtgctaaat ggagccttat ttttaaaaag actagcagct agtttttcta cagaccccgc ttctgttctt tctgagattc caataaccaa aagatcttta tttaaaacaa actcatcccc accttccaaa gaagtttctt cccatctatt aaaccaaatt ggaacatttt ctttgtaagc ggaatgatat ttaaaaatat actctgcaaa tattgtctct ctacgtctaa ccttggtata cattttattt attgtaattc cattgccaat actggcaaaa ggatctctgg taaataaaac attgggcata ggatcaataa caaaaagact tgaaccatta acccaatcat caagcgaaaa ttcacaatct ttaagctctt ctcttgcaac gccggaaatc attttagaaa ccatattatc aacggttaaa ttagaaaaat aatcttttaa aatattaatt acaccatctg tttttatttc tgcttccaga ataaattgag atataaattt atttttgagc gctacagaag aagcaagaac ttcactaaca agatcctcaa catactcaat ttcaactgaa ttatctttta aaatatttac aaaaacttca tgctcttgtc ttgcaacttt aagataagga atatcatcaa ataaaaaatt tttcataatc aagggtgtca aattttctaa ttcttctcct ggcctatgaa gcaaaacttt tttcaaacga cctatttccg aaaatatatt tattggattt aaatattctt cttccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aagaatattt aaatccaata aatatatttt cggaaatagg tcgtttgaaa aaagttttgc ttcataggcc aggagaagaa ttagaaaatt tgacaccctt gattatgaaa aattttttat ttgatgatat tccttatctt aaagttgcaa gacaagagca tgaagttttt gtaaatattt taaaagataa ttcagttgaa attgagtatg ttgaggatct tgttagtgaa gttcttgctt cttctgtagc gctcaaaaat aaatttatat ctcaatttat tctggaagca gaaataaaaa cagatggtgt aattaatatt ttaaaagatt atttttctaa tttaaccgtt gataatatgg tttctaaaat gatttccggc gttgcaagag aagagcttaa agattgtgaa ttttcgcttg atgattgggt taatggttca agtctttttg ttattgatcc tatgcccaat gttttattta ccagagatcc ttttgccagt attggcaatg gaattacaat aaataaaatg tataccaagg ttagacgtag agagacaata tttgcagagt atatttttaa atatcattcc gcttacaaag aaaatgttcc aatttggttt aatagatggg aagaaacttc tttggaaggt ggggatgagt ttgttttaaa taaagatctt ttggttattg gaatctcaga aagaacagaa gcggggtctg tagaaaaact agctgctagt ctttttaaaa ataaggctcc atttagcaca attttggctt ttaaaattcc aaaaaacaga gcctatatgc acttagacac agtttttacc caaattgatt atagcgtttt tacaagtttt acaagtgatg atatgtattt ctcaatttat gttttaactt acaattcaaa ttctaataaa attaatatta aaaaagaaaa agccaagctt aaagatgttt tgagctttta tttaggcaga aaaattgaca taataaaatg tgcgggtgga gatttaatac atggtgcaag agaacaatgg aatgatggtg ctaatgtttt ggcgatagct ccaggagaag taattgctta ttctagaaat catgtaacta ataagctgtt tgaagaaaat ggtattaaag ttcacagaat tccgtctagc gagctttcaa ggggtcgtgg tgggccaaga tgcatgtcta tgtctttagt aagagaggat atttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat