SSGCID: Target Page

BobuA.18044.a

general stress protein (ctc) (BB_0786)

CENTER ID:	BobuA.18044.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.18044.a.B1.GE39226	full length		1	182

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.1178
RefSeq:	NP_212920.2
UniProt:	O51727

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MENSRVLSCQ YRSSFGSSNA RRIRAKSEIP AVVYGQGKDV SHLRIKSSEF NKKFAKFTDN TVLILDDGKL ERCVFVKDAA ENIASKLIYH IDFYEVDRNV ELEKYVPIKL IGASIGVKEG GILTVLKEQV KVRSLPLDLP EFIELDLTPV NKGDSVLLKD LVLPSNVRLA ENDENLEVVI IK

NT Sequence

gtggaaaata gcagggtttt aagttgccaa tatagatcaa gctttggatc ttctaatgct cgcagaataa gagctaagtc tgaaatacca gctgttgttt acgggcaagg taaggatgtt tcacacttga gaattaagag tagtgaattt aataagaaat ttgcaaaatt tacggataat actgtcttaa tattagatga tggcaagctt gaaagatgtg tttttgttaa agatgctgca gaaaatattg ccagcaagct catttatcat attgattttt acgaagtaga taggaatgtt gagcttgaaa aatatgttcc tattaagctt attggagctt ctattggggt taaagaaggt ggaattttga ctgttttaaa agagcaggtt aaggtaaggt ctttgccctt ggatttgcca gaattcatag agcttgattt aactcctgtt aataaaggag atagcgttct tcttaaagat cttgtgttgc cttctaatgt taggcttgca gaaaatgacg agaatttgga agttgttatt ataaagtga

Details for BobuA.18044.a.B1.GE39226

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/5/2015
EXPECTED MW:	21kDa
OBSERVED MW:	79kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV ENSRVLSCQY RSSFGSSNAR RIRAKSEIPA VVYGQGKDVS HLRIKSSEFN KKFAKFTDNT VLILDDGKLE RCVFVKDAAE NIASKLIYHI DFYEVDRNVE LEKYVPIKLI GASIGVKEGG ILTVLKEQVK VRSLPLDLPE SIELDLTPVN KGDSVLLKDL VLPSNVRLAE NDENLEVVII K

Validated NT Sequence

atcttactca cttactttat aataacaact tccaaattct cgtcattttc tgcaagccta acattagaag gcaacacaag atctttaaga agaacgctat ctcctttatt aacaggagtt aaatcaagct ctatggattc tggcaaatcc aagggcaaag accttacctt aacctgctct tttaaaacag tcaaaattcc accttcttta accccaatag aagctccaat tagcttaata ggaacatatt tttcaagctc aacattccta tctacttcgt aaaaatcaat atgataaatg agcttgctgg caatattttc tgcagcatct ttaacaaaaa cacatctttc aagcttgcca tcatctaata ttaagacagt attatccgta aattttgcaa atttcttatt aaattcacta ctcttaattc tcaagtgtga aacatcctta ccttgcccgt aaacaacagc tggtatttca gacttagctc ttattctgcg agcattagaa gatccaaagc ttgatctata ttggcaactt aaaaccctgc tattttccac catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHME NSRVLSCQYR SSFGSSNARR IRAKSEIPAV VYGQGKDVSH LRIKSSEFNK KFAKFTDNTV LILDDGKLER CVFVKDAAEN IASKLIYHID FYEVDRNVEL EKYVPIKLIG ASIGVKEGGI LTVLKEQVKV RSLPLDLPEF IELDLTPVNK GDSVLLKDLV LPSNVRLAEN DENLEVVIIK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggaaa atagcagggt tttaagttgc caatatagat caagctttgg atcttctaat gctcgcagaa taagagctaa gtctgaaata ccagctgttg tttacgggca aggtaaggat gtttcacact tgagaattaa gagtagtgaa tttaataaga aatttgcaaa atttacggat aatactgtct taatattaga tgatggcaag cttgaaagat gtgtttttgt taaagatgct gcagaaaata ttgccagcaa gctcatttat catattgatt tttacgaagt agataggaat gttgagcttg aaaaatatgt tcctattaag cttattggag cttctattgg ggttaaagaa ggtggaattt tgactgtttt aaaagagcag gttaaggtaa ggtctttgcc cttggatttg ccagaattca tagagcttga tttaactcct gttaataaag gagatagcgt tcttcttaaa gatcttgtgt tgccttctaa tgttaggctt gcagaaaatg acgagaattt ggaagttgtt attataaagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat