BobuA.18032.a

ATP-dependent Clp protease subunit A (BB_0369)

CENTER ID: BobuA.18032.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.18032.a.B1.GE39225 full length 1 739
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.764
RefSeq: NP_212503.2
RefSeq: WP_010889736.1
UniProt: O51342

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTEEHIFYG LIKSDKVKEL LNLCAIDFYK LNKQLEEFFS KLPLRGNYIP DYVSSMDYLY DDIISVLFFY KKPYKIQEKD LLWVLVKKRK NSILDALLSS GFNLTIFDKL IEAHDYLAVN TKSASGDSEL IAEYIHNNAP KRKGGFHIFD DKRDELDQNN IFLESKDSIG NFLTNVIDTL DLKYNPLIGR SQELSRLIQV ILRKHKSNPI LFGEPGVGKT VLIQGLAYKI KIENVPKDLI GYEIYSLDIG RLVSGTKYRG DLESRVNRVL DFLSSRKKVM LFIDEIHMIV GAGATSFGSM DISNLLKPIL TLGKIKFIGA TTEYEYRKFF LKDKALMRRF QSIELKEPNF EDAYNILQEI KKDYERYHNV EYTDEAIQAC ILMSQKYIKD RFLPDKAFDI LDELGSKFKL ENIKRIITKD DVCDLIKSIV GSNIFNFEEY DGELLINLEN RIKKELIIHD NLVLDLILNI KLLKFNLLAN RSTIGIFAFI GASGSGKCKL MDILSEEFKI PKFSLNMGEY NDFNSLDRLI GPVLSNEGYY ESTRFFKFLN KSSNSIIFLS DFDKCNKRVL DFFLEGFKTG KLFDGLGKKV SLSESLIVIS INAESKELNS IGFRNKMAGE NDFNFILKKR LPNEFLELID HVFVFKSIDE LDFEKIIFNE LNYFARILRD RKFDVFFEKS VVDYIREKIY GKGYSLKSVR KFIFKELGKL LIDEILFKKI ENSGKIKIYL DETIKYEFL
NT Sequence
gtgtttacag aagagcacat tttttatggg cttattaaaa gtgataaagt taaagaacta cttaatttgt gtgcaattga tttttataaa cttaataaac aactagaaga attttttagt aaacttccct taagaggcaa ttatatccca gactatgttt ctagtatgga ttatttgtat gacgatataa ttagtgttct ttttttttat aaaaaacctt ataaaataca agaaaaagat ctattgtggg tgcttgtcaa aaaaagaaaa aatagtattt tagatgcgct gcttagctcg ggttttaatt tgactatttt tgataaactt attgaagctc atgattattt agctgtaaat actaaatctg cctcaggcga cagtgaatta attgcagaat atattcataa taatgcgcca aaaaggaaag gaggctttca tatttttgat gataagcgtg atgaattgga tcaaaataat attttcttag aaagtaaaga ctctattggt aattttttaa caaacgttat tgatactttg gatttaaaat acaatccttt aattggtaga agtcaagaat tatctcggtt aatccaggtg atacttagga agcataaaag taatcctatt ttgtttggag agcctggtgt tggaaaaaca gtattaatcc aaggtcttgc atataaaata aaaatagaga atgttccaaa ggatttaata gggtatgaaa tctattctct tgatattggt aggcttgttt cgggtactaa atatagggga gatcttgaga gtagggtgaa tagggtttta gattttttaa gctcaagaaa aaaagttatg ctttttattg atgaaatcca tatgatagta ggggcagggg ctacttcatt tggcagtatg gatatttcca atttgttaaa acccattctc actttaggaa aaattaaatt tattggagct actacagaat atgaatatag aaaatttttt ttaaaagata aggccttaat gagaaggttt cagagtatag agctcaaaga gcctaatttt gaagacgctt ataatatttt gcaggagatt aaaaaagatt acgagaggta tcataatgtg gaatatacag acgaggcaat acaagcttgc attctcatgt ctcaaaaata tattaaagat agatttcttc cagacaaagc ttttgatatt ttagatgaat taggctctaa gtttaagctt gaaaatataa aaaggattat aacaaaagat gatgtttgcg atctgattaa atctattgtt ggttctaata tttttaattt tgaagagtat gatggtgaat tgctaattaa tttagaaaat agaataaaaa aagaacttat tatacatgat aacttggtac ttgatttgat attaaatatt aaattattaa aattcaattt gcttgccaat agaagtacta ttggaatatt tgcctttatt ggtgcttctg ggtcaggaaa atgcaaattg atggatattt tatcagaaga gtttaaaatt cctaaattta gtcttaacat gggtgagtat aatgatttta attctcttga tagattgatt gggcctgttt taagtaatga aggatattat gaatctacca gattttttaa atttttgaac aaatcttcta attctattat tttcctatca gattttgata aatgtaataa aagggtttta gatttttttt tagaggggtt taaaacaggt aaactttttg atggtcttgg aaaaaaggta agcttatcag aaagtttaat agtaataagt atcaatgctg agagcaaaga gcttaatagc attggtttta gaaataaaat ggcgggggaa aatgatttta actttatatt aaagaagaga ttgcccaatg agtttttaga attaatagat catgtgtttg tatttaaatc tattgatgag ttagattttg aaaaaatcat ttttaatgaa cttaattatt ttgcaaggat attaagagat agaaaatttg atgttttttt tgaaaaaagt gttgttgatt atattcgaga aaagatttat ggaaaggggt acagcttaaa aagtgttaga aaatttatat tcaaagaatt gggaaagctt ttaatagatg aaattctttt taagaaaatt gaaaattctg gtaaaataaa aatctattta gatgaaacaa taaaatatga gtttttataa
Details for BobuA.18032.a.B1.GE39225
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 87kDa
OBSERVED MW: 79kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMF TEEHIFYGLI KSDKVKELLN LCAIDFYKLN KQLEEFFSKL PLRGNYIPDY VSSMDYLYDD IISVLFFYKK PYKIQEKDLL WVLVKKRKNS ILDALLSSGF NLTIFDKLIE AHDYLAVNTK SASGDSELIA EYIHNNAPKR KGGFHIFDDK RDELDQNNIF LESKDSIGNF LTNVIDTLDL KYNPLIGRSQ ELSRLIQVIL RKHKSNPILF GEPGVGKTVL IQGLAYKIKI ENVPKDLIGY EIYSLDIGRL VSGTKYRGDL ESRVNRVLDF LSSRKKVMLF IDEIHMIVGA GATSFGSMDI SNLLKPILTL GKIKFIGATT EYEYRKFFLK DKALMRRFQS IELKEPNFED AYNILQEIKK DYERYHNVEY TDEAIQACIL MSQKYIKDRF LPDKAFDILD ELGSKFKLEN IKRIITKDDV CDLIKSIVGS NIFNFEEYDG ELLINLENRI KKELIIHDNL VLDLILNIKL LKFNLLANRS TIGIFAFIGA SGSGKCKLMD ILSEEFKIPK FSLNMGEYND FNSLDRLIGP VLSNEGYYES TRFFKFLNKS SNSIIFLSDF DKCNKRVLDF FLEGFKTGKL FDGLGKKVSL SESLIVISIN AESKELNSIG FRNKMAGEND FNFILKKRLP NEFLELIDHV FVFKSIDELD FEKIIFNELN YFARILRDRK FDVFFEKSVV DYIREKIYGK GYSLKSVRKF IFKELGKLLI DEILFKKIEN SGKIKIYLDE TIKYEFL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgttta cagaagagca cattttttat gggcttatta aaagtgataa agttaaagaa ctacttaatt tgtgtgcaat tgatttttat aaacttaata aacaactaga agaatttttt agtaaacttc ccttaagagg caattatatc ccagactatg tttctagtat ggattatttg tatgacgata taattagtgt tctttttttt tataaaaaac cttataaaat acaagaaaaa gatctattgt gggtgcttgt caaaaaaaga aaaaatagta ttttagatgc gctgcttagc tcgggtttta atttgactat ttttgataaa cttattgaag ctcatgatta tttagctgta aatactaaat ctgcctcagg cgacagtgaa ttaattgcag aatatattca taataatgcg ccaaaaagga aaggaggctt tcatattttt gatgataagc gtgatgaatt ggatcaaaat aatattttct tagaaagtaa agactctatt ggtaattttt taacaaacgt tattgatact ttggatttaa aatacaatcc tttaattggt agaagtcaag aattatctcg gttaatccag gtgatactta ggaagcataa aagtaatcct attttgtttg gagagcctgg tgttggaaaa acagtattaa tccaaggtct tgcatataaa ataaaaatag agaatgttcc aaaggattta atagggtatg aaatctattc tcttgatatt ggtaggcttg tttcgggtac taaatatagg ggagatcttg agagtagggt gaatagggtt ttagattttt taagctcaag aaaaaaagtt atgcttttta ttgatgaaat ccatatgata gtaggggcag gggctacttc atttggcagt atggatattt ccaatttgtt aaaacccatt ctcactttag gaaaaattaa atttattgga gctactacag aatatgaata tagaaaattt tttttaaaag ataaggcctt aatgagaagg tttcagagta tagagctcaa agagcctaat tttgaagacg cttataatat tttgcaggag attaaaaaag attacgagag gtatcataat gtggaatata cagacgaggc aatacaagct tgcattctca tgtctcaaaa atatattaaa gatagatttc ttccagacaa agcttttgat attttagatg aattaggctc taagtttaag cttgaaaata taaaaaggat tataacaaaa gatgatgttt gcgatctgat taaatctatt gttggttcta atatttttaa ttttgaagag tatgatggtg aattgctaat taatttagaa aatagaataa aaaaagaact tattatacat gataacttgg tacttgattt gatattaaat attaaattat taaaattcaa tttgcttgcc aatagaagta ctattggaat atttgccttt attggtgctt ctgggtcagg aaaatgcaaa ttgatggata ttttatcaga agagtttaaa attcctaaat ttagtcttaa catgggtgag tataatgatt ttaattctct tgatagattg attgggcctg ttttaagtaa tgaaggatat tatgaatcta ccagattttt taaatttttg aacaaatctt ctaattctat tattttccta tcagattttg ataaatgtaa taaaagggtt ttagattttt ttttagaggg gtttaaaaca ggtaaacttt ttgatggtct tggaaaaaag gtaagcttat cagaaagttt aatagtaata agtatcaatg ctgagagcaa agagcttaat agcattggtt ttagaaataa aatggcgggg gaaaatgatt ttaactttat attaaagaag agattgccca atgagttttt agaattaata gatcatgtgt ttgtatttaa atctattgat gagttagatt ttgaaaaaat catttttaat gaacttaatt attttgcaag gatattaaga gatagaaaat ttgatgtttt ttttgaaaaa agtgttgttg attatattcg agaaaagatt tatggaaagg ggtacagctt aaaaagtgtt agaaaattta tattcaaaga attgggaaag cttttaatag atgaaattct ttttaagaaa attgaaaatt ctggtaaaat aaaaatctat ttagatgaaa caataaaata tgagttttta tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat