BobuA.17987.a
DNA polymerase III, subunit beta (dnaN) (BB_0438)
| CENTER ID: | BobuA.17987.a |
| ORGANISM: | Borrelia burgdorferi B31 |
| ASSOCIATED DISEASE: | Lyme disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.17987.a.B1.GE39223 | full length | 1 | 385 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BobuA.17987.a.B1.PW37766 | full length | 1 | 385 |
Structures
6DJ8
DEPOSITED: 5/24/2018
DETERMINATION: XRay
CLONE: BobuA.17987.a.B1.GE39223
PROTEIN: BobuA.17987.a.B1.PW37766
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|224326.49.peg.829 |
| RefSeq: | NP_212572.1 |
| UniProt: | P33761 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLHNTFFICE TNQIMNEIEK AKGIILNRNM NDIWSALLIE VKKSNLIIKS TDRNIFFEST ISIVSETDFK VLINASNFYD AVKAFNFYKK IKIVFNENNS KLEIMGELND EKEEYEDHLK EPTFSYEEIE NYNYDMVNED YTFGIEIKQK SFKKVINRIA FSAHLDESKN VLNGVYFSKD EDSKLLLVST NGHRMSICKT EVIVEEDVNF IVPVKIFNFL KHLMSGEGMV KIKFSDKKFY VEFDNYKIAC SLINGNYPDY KSIIPKEQKN KSLVSLGILK DRLARVNLYV DKSRKLVLTF SELQLKLLGE DLITGRKGEF FIKDPNYLYD GADEVMAINI SYFVEAISVF ETSKIEIQFN SGNVLKLSEP ENFNFTHLIM PMSLG
NT Sequence
atgctacaca acacattttt tatttgcgaa acaaatcaaa ttatgaatga gatagaaaaa gcaaagggaa taatattaaa cagaaatatg aatgatattt ggagtgcatt gttaatagaa gtaaaaaaat ctaatctcat aatcaaatca acagacagaa atatattttt tgaaagcaca atttcaattg tttcagaaac agattttaag gtattaatta atgcttcaaa tttttatgat gcagtaaaag cattcaattt ttataagaaa atcaaaatag tttttaatga aaataatagt aaattagaaa ttatgggaga gttaaatgat gagaaagagg aatatgaaga tcatttaaaa gagcccactt tttcatacga agaaatagaa aattacaatt atgatatggt taatgaagat tatacttttg gaattgaaat aaaacaaaaa tcttttaaaa aagtaataaa tagaatagct ttttcagcac atcttgatga atctaagaat gtattaaatg gtgtgtattt ttcaaaagat gaagattcta aattactact tgtttctact aatggtcaca gaatgtctat ttgtaaaaca gaagtcatag ttgaagaaga tgtgaatttt atagttcctg taaaaatatt taatttctta aaacatctta tgtcgggaga aggtatggtt aaaataaagt tttcggataa aaaattttat gttgaatttg ataattataa aatagcttgt agcttaatta atggtaatta tccggattat aaaagcatta tacctaaaga acagaaaaat aaatctttag tttcgctagg cattttaaaa gatagacttg caagagtcaa tttatatgtt gataaatcaa gaaagttggt tttaactttc tctgagttac aattaaaact tcttggagaa gatttgatta ctggaagaaa aggtgaattt ttcattaaag atccaaatta tctgtatgac ggagcagatg aggttatggc tattaatatt tcatattttg ttgaagctat tagtgttttt gaaacttcaa agatagaaat acaatttaat tcgggcaatg tattaaaatt gagcgaacct gaaaatttta attttacgca cttgataatg ccgatgtctt tgggttaa
Details for BobuA.17987.a.B1.GE39223
| HARVESTED ON: | 6/10/2015 |
| SEQUENCED ON: | 6/5/2015 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 91 |
Validated AA Sequence
MNEIEKAKGI ILNRNMNDIW SALLIEVKKS NLIIKSTDRN IFFESTISIV SETDFKVLIN ASNFYDAVKA FSFYKKIKIV FNENNSKLEI MGELNDEKEE YEDHLKEPTF SYEEIENYNY DMVNEDYTFG IEIKQKSFKK VINRIAFSAH LDESKNVLNG VYFSKDEDSK LLLVSTNGHR MSICKTEVIV EEDVNFIVPV KIFNFLKHLM SGEGMVKIKF SDKKFYVEFD NYKIACSLIN GNYPDYKSII PKEQKNKSLV SLGILKDRLA RVNLYVDKSR KLVLTFSELQ LKLLGEDLIT GRKGEFFIKD PNYLYDGADE VMAINISYFV EAISVFETSK IEIQFNSGNV LKLSEPENFN F
Validated NT Sequence
gtaaaattaa aattttcagg ttcgctcaat tttaatacat tgcccgaatt aaattgtatt tctatctttg aagtttcaaa aacactaata gcttcaacaa aatatgaaat attaatagcc ataacctcat ctgctccgtc atacagataa tttggatctt taatgaaaaa ttcacctttt cttccagtaa tcaaatcttc tccaagaagt tttaattgta actcagagaa agttaaaacc aactttcttg atttatcaac atataaattg actcttgcaa gtctatcttt taaaatgcct agcgaaacta aagatttatt tttctgttct ttaggtataa tgcttttata atccggataa ttaccattaa ttaagctaca agctatttta taattatcaa attcaacata aaatttttta tccgaaaact ttattttaac cataccttct cccgacataa gatgttttaa gaaattaaat atttttacag gaactataaa attcacatct tcttcaacta tgacttctgt tttacaaata gacattctgt gaccattagt agaaacaagt agtaatttag aatcttcatc ttttgaaaaa tacacaccat ttaatacatt cttagattca tcaagatgtg ctgaaaaagc tattctattt attacttttt taaaagattt ttgttttatt tcaattccaa aagtataatc ttcattaacc atatcataat tgtaattttc tatttcttcg tatgaaaaag tgggctcttt taaatgatct tcatattcct ctttctcatc atttaactct cccataattt ctaatttact attattttca ttaaaaacta ttttgatttt cttataaaaa ctgaatgctt ttactgcatc ataaaaattt gaagcattaa ttaatacctt aaaatctgtt tctgaaacaa ttgaaattgt gctttcaaaa aatatatttc tgtctgttga tttgattatg agattagatt tttttacttc tattaacaat gcactccaaa tatcattcat atttctgttt aatattattc cctttgcttt ttctatctca ttcataattt gatttgtttc gcaaataaaa aatgt
Expected Protein Sequence
MAHHHHHHML HNTFFICETN QIMNEIEKAK GIILNRNMND IWSALLIEVK KSNLIIKSTD RNIFFESTIS IVSETDFKVL INASNFYDAV KAFNFYKKIK IVFNENNSKL EIMGELNDEK EEYEDHLKEP TFSYEEIENY NYDMVNEDYT FGIEIKQKSF KKVINRIAFS AHLDESKNVL NGVYFSKDED SKLLLVSTNG HRMSICKTEV IVEEDVNFIV PVKIFNFLKH LMSGEGMVKI KFSDKKFYVE FDNYKIACSL INGNYPDYKS IIPKEQKNKS LVSLGILKDR LARVNLYVDK SRKLVLTFSE LQLKLLGEDL ITGRKGEFFI KDPNYLYDGA DEVMAINISY FVEAISVFET SKIEIQFNSG NVLKLSEPEN FNFTHLIMPM SLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctac acaacacatt ttttatttgc gaaacaaatc aaattatgaa tgagatagaa aaagcaaagg gaataatatt aaacagaaat atgaatgata tttggagtgc attgttaata gaagtaaaaa aatctaatct cataatcaaa tcaacagaca gaaatatatt ttttgaaagc acaatttcaa ttgtttcaga aacagatttt aaggtattaa ttaatgcttc aaatttttat gatgcagtaa aagcattcaa tttttataag aaaatcaaaa tagtttttaa tgaaaataat agtaaattag aaattatggg agagttaaat gatgagaaag aggaatatga agatcattta aaagagccca ctttttcata cgaagaaata gaaaattaca attatgatat ggttaatgaa gattatactt ttggaattga aataaaacaa aaatctttta aaaaagtaat aaatagaata gctttttcag cacatcttga tgaatctaag aatgtattaa atggtgtgta tttttcaaaa gatgaagatt ctaaattact acttgtttct actaatggtc acagaatgtc tatttgtaaa acagaagtca tagttgaaga agatgtgaat tttatagttc ctgtaaaaat atttaatttc ttaaaacatc ttatgtcggg agaaggtatg gttaaaataa agttttcgga taaaaaattt tatgttgaat ttgataatta taaaatagct tgtagcttaa ttaatggtaa ttatccggat tataaaagca ttatacctaa agaacagaaa aataaatctt tagtttcgct aggcatttta aaagatagac ttgcaagagt caatttatat gttgataaat caagaaagtt ggttttaact ttctctgagt tacaattaaa acttcttgga gaagatttga ttactggaag aaaaggtgaa tttttcatta aagatccaaa ttatctgtat gacggagcag atgaggttat ggctattaat atttcatatt ttgttgaagc tattagtgtt tttgaaactt caaagataga aatacaattt aattcgggca atgtattaaa attgagcgaa cctgaaaatt ttaattttac gcacttgata atgccgatgt ctttgggttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BobuA.17987.a.B1.PW37766
| PURIFICATION DATe: | 8/11/2015 |
| CONCENTRATION: | 32mg/ml |
| OBSERVED MW: | 47kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 91 |
Protocol Notes
notes unavailable
Validated AA Sequence
MNEIEKAKGI ILNRNMNDIW SALLIEVKKS NLIIKSTDRN IFFESTISIV SETDFKVLIN ASNFYDAVKA FSFYKKIKIV FNENNSKLEI MGELNDEKEE YEDHLKEPTF SYEEIENYNY DMVNEDYTFG IEIKQKSFKK VINRIAFSAH LDESKNVLNG VYFSKDEDSK LLLVSTNGHR MSICKTEVIV EEDVNFIVPV KIFNFLKHLM SGEGMVKIKF SDKKFYVEFD NYKIACSLIN GNYPDYKSII PKEQKNKSLV SLGILKDRLA RVNLYVDKSR KLVLTFSELQ LKLLGEDLIT GRKGEFFIKD PNYLYDGADE VMAINISYFV EAISVFETSK IEIQFNSGNV LKLSEPENFN F
Validated NT Sequence
gtaaaattaa aattttcagg ttcgctcaat tttaatacat tgcccgaatt aaattgtatt tctatctttg aagtttcaaa aacactaata gcttcaacaa aatatgaaat attaatagcc ataacctcat ctgctccgtc atacagataa tttggatctt taatgaaaaa ttcacctttt cttccagtaa tcaaatcttc tccaagaagt tttaattgta actcagagaa agttaaaacc aactttcttg atttatcaac atataaattg actcttgcaa gtctatcttt taaaatgcct agcgaaacta aagatttatt tttctgttct ttaggtataa tgcttttata atccggataa ttaccattaa ttaagctaca agctatttta taattatcaa attcaacata aaatttttta tccgaaaact ttattttaac cataccttct cccgacataa gatgttttaa gaaattaaat atttttacag gaactataaa attcacatct tcttcaacta tgacttctgt tttacaaata gacattctgt gaccattagt agaaacaagt agtaatttag aatcttcatc ttttgaaaaa tacacaccat ttaatacatt cttagattca tcaagatgtg ctgaaaaagc tattctattt attacttttt taaaagattt ttgttttatt tcaattccaa aagtataatc ttcattaacc atatcataat tgtaattttc tatttcttcg tatgaaaaag tgggctcttt taaatgatct tcatattcct ctttctcatc atttaactct cccataattt ctaatttact attattttca ttaaaaacta ttttgatttt cttataaaaa ctgaatgctt ttactgcatc ataaaaattt gaagcattaa ttaatacctt aaaatctgtt tctgaaacaa ttgaaattgt gctttcaaaa aatatatttc tgtctgttga tttgattatg agattagatt tttttacttc tattaacaat gcactccaaa tatcattcat atttctgttt aatattattc cctttgcttt ttctatctca ttcataattt gatttgtttc gcaaataaaa aatgt
Expressed Protein Sequence
MAHHHHHHML HNTFFICETN QIMNEIEKAK GIILNRNMND IWSALLIEVK KSNLIIKSTD RNIFFESTIS IVSETDFKVL INASNFYDAV KAFNFYKKIK IVFNENNSKL EIMGELNDEK EEYEDHLKEP TFSYEEIENY NYDMVNEDYT FGIEIKQKSF KKVINRIAFS AHLDESKNVL NGVYFSKDED SKLLLVSTNG HRMSICKTEV IVEEDVNFIV PVKIFNFLKH LMSGEGMVKI KFSDKKFYVE FDNYKIACSL INGNYPDYKS IIPKEQKNKS LVSLGILKDR LARVNLYVDK SRKLVLTFSE LQLKLLGEDL ITGRKGEFFI KDPNYLYDGA DEVMAINISY FVEAISVFET SKIEIQFNSG NVLKLSEPEN FNFTHLIMPM SLG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTAC ACAACACATT TTTTATTTGC GAAACAAATC AAATTATGAA TGAGATAGAA AAAGCAAAGG GAATAATATT AAACAGAAAT ATGAATGATA TTTGGAGTGC ATTGTTAATA GAAGTAAAAA AATCTAATCT CATAATCAAA TCAACAGACA GAAATATATT TTTTGAAAGC ACAATTTCAA TTGTTTCAGA AACAGATTTT AAGGTATTAA TTAATGCTTC AAATTTTTAT GATGCAGTAA AAGCATTCAA TTTTTATAAG AAAATCAAAA TAGTTTTTAA TGAAAATAAT AGTAAATTAG AAATTATGGG AGAGTTAAAT GATGAGAAAG AGGAATATGA AGATCATTTA AAAGAGCCCA CTTTTTCATA CGAAGAAATA GAAAATTACA ATTATGATAT GGTTAATGAA GATTATACTT TTGGAATTGA AATAAAACAA AAATCTTTTA AAAAAGTAAT AAATAGAATA GCTTTTTCAG CACATCTTGA TGAATCTAAG AATGTATTAA ATGGTGTGTA TTTTTCAAAA GATGAAGATT CTAAATTACT ACTTGTTTCT ACTAATGGTC ACAGAATGTC TATTTGTAAA ACAGAAGTCA TAGTTGAAGA AGATGTGAAT TTTATAGTTC CTGTAAAAAT ATTTAATTTC TTAAAACATC TTATGTCGGG AGAAGGTATG GTTAAAATAA AGTTTTCGGA TAAAAAATTT TATGTTGAAT TTGATAATTA TAAAATAGCT TGTAGCTTAA TTAATGGTAA TTATCCGGAT TATAAAAGCA TTATACCTAA AGAACAGAAA AATAAATCTT TAGTTTCGCT AGGCATTTTA AAAGATAGAC TTGCAAGAGT CAATTTATAT GTTGATAAAT CAAGAAAGTT GGTTTTAACT TTCTCTGAGT TACAATTAAA ACTTCTTGGA GAAGATTTGA TTACTGGAAG AAAAGGTGAA TTTTTCATTA AAGATCCAAA TTATCTGTAT GACGGAGCAG ATGAGGTTAT GGCTATTAAT ATTTCATATT TTGTTGAAGC TATTAGTGTT TTTGAAACTT CAAAGATAGA AATACAATTT AATTCGGGCA ATGTATTAAA ATTGAGCGAA CCTGAAAATT TTAATTTTAC GCACTTGATA ATGCCGATGT CTTTGGGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT