BobuA.17982.a

CTP synthase (pyrG) (BB_0575)

CENTER ID: BobuA.17982.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17982.a.B2.GE39221 mature protein 25 533
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.966
RefSeq: NP_212709.1
UniProt: O51522

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKNLKILVI TGGVISGIGK GVTSASIARL FRYDFRVTPI KCDGYLNTDP GTINPVEHGE VFVLDDGGEV DMDFGHYERF LNLNAKSSWN ITMGKIYKKI LENERKGKYL GRTVQLIPHV TDEIKSTIFQ IASSENSDML IIEIGGTVGD MENILFIETV RQIRQEIGSG NISFIHLTYV PSPAGINEQK SKPTQQSVKT LNKAGIFPDL IIARSSQVLT DQIRKKVAMF CNVESTSIID NVDVSTIYEI PISFYKQGVH EILSSKLNIK VDPKIEELSK LVGVIKSNFF VPKKIINIAI CGKYAELDDS YASIRESLVH VAAHLDLLIK STLIDSNDLN ESCLKEFDGI IVPGGFGGKG YEGKIMAIKY ARENNIPFLG ICLGLQLAVI EFARNVCGIL DADTEENLAR DKPLKSPVIH LLPEQKGIKD KGATMRLGGY PVILKKNTIA FKLYGQDRII ERFRHRYEVN NDYIDLFAKN GLIVSGFSSD FKMAKLIEIP ENKFFVACQF HPELITRIEN PAKLFLGLIK ACI
NT Sequence
atgaaaaaaa acttaaagat tttagtaata acaggaggcg tgatctctgg aattggtaaa ggagttacat cggcaagtat tgcaaggttg tttagatatg attttagagt tactccaatt aaatgtgatg ggtatttaaa tactgatcct ggtactatta atcctgttga gcacggagaa gtttttgtgc ttgatgatgg aggagaggtt gatatggact ttggtcatta cgagaggttt ttaaatctta atgccaagtc tagttggaac attacaatgg gcaaaatata caaaaagata cttgaaaatg agcgaaaggg taaatatttg ggaagaacag ttcagcttat tccccatgtt actgatgaga tcaaatctac aatttttcag attgcaagtt ctgagaatag tgatatgttg ataattgaaa ttggtggaac cgtaggagat atggaaaata ttttatttat tgagacagta agacaaataa gacaggagat tgggagtggt aatatttctt ttattcattt aacatatgtg ccaagtccag ctggaattaa tgagcaaaaa tctaaaccta ctcaacaaag tgttaaaacc ttaaataaag caggtatttt ccccgattta attattgcta gaagttcaca agtattgaca gaccaaatca gaaaaaaagt ggcaatgttt tgcaatgttg agagcacttc tattattgac aatgttgatg tttctactat ttatgaaatt cctatatctt tttataagca gggtgtacat gagattttaa gctctaagtt aaatattaag gttgatccaa aaatagaaga gctttcaaag cttgtaggag ttataaaatc taattttttt gtgcctaaaa aaattattaa tattgctatt tgtggtaaat atgctgaact tgatgattct tatgcatcaa ttagagagtc tttggttcat gttgcagccc atttggattt gcttattaaa agcactttaa ttgattctaa tgatttaaat gagagctgtt taaaagagtt tgacggcatt attgttcctg gcggctttgg aggcaaagga tatgaaggta aaattatggc tattaaatat gctcgtgaga ataatattcc ctttcttgga atttgtcttg gtttgcagct tgctgtaata gaatttgctc gtaatgtttg tggaatactt gatgctgata cggaggaaaa tttagcaaga gacaagccct taaaaagtcc tgttatccat ttacttcctg agcaaaaggg aattaaagat aagggcgcta caatgaggct tggtggatat cctgtgattc ttaaaaagaa tacaatagct tttaaacttt atggccaaga tcggataatt gaaagattta gacataggta tgaagtcaat aatgattata tagatttatt tgcaaaaaat gggcttatag tatctggatt ttcaagtgat tttaaaatgg caaaattaat agaaattcct gaaaataaat ttttcgtagc ttgccagttt catccagaac ttattacaag aatagaaaat ccagccaagc tttttctagg attaattaaa gcttgtattt ga
Details for BobuA.17982.a.B2.GE39221
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA SIARLFRYDF RVTPIKCDGY LNTDPGTINP VEHGEVFVLD DGGEVDMDFG HYERFLNLNA KSSWNITMGK IYKKILENER KGKYLGRTVQ LIPHVTDEIK STIFQIASSE NSDMLIIEIG GTVGDMENIL FIETVRQIRQ EIGSGNISFI HLTYVPSPAG INEQKSKPTQ QSVKTLNKAG IFPDLIIARS SQVLTDQIRK KVAMFCNVES TSIIDNVDVS TIYEIPISFY KQGVHEILSS KLNIKVDPKI EELSKLVGVI KSNFFVPKKI INIAICGKYA ELDDSYASIR ESLVHVAAHL DLLIKSTLID SNDLNESCLK EFDGIIVPGG FGGKGYEGKI MAIKYARENN IPFLGICLGL QLAVIEFARN VCGILDADTE ENLARDKPLK SPVIHLLPEQ KGIKDKGATM RLGGYPVILK KNTIAFKLYG QDRIIERFRH RYEVNNDYID LFAKNGLIVS GFSSDFKMAK LIEIPENKFF VACQFHPELI TRIENPAKL
Validated NT Sequence
aaaaagcttg gctggatttt ctattcttgt aataagttct ggatgaaact ggcaagctac gaaaaattta ttttcaggaa tttctattaa ttttgccatt ttaaaatcac ttgaaaatcc agatactata agcccatttt ttgcaaataa atctatataa tcattattga cttcatacct atgtctaaat ctttcaatta tccgatcttg gccataaagt ttaaaagcta ttgtattctt tttaagaatc acaggatatc caccaagcct cattgtagcg cccttatctt taattccctt ttgctcagga agtaaatgga taacaggact ttttaagggc ttgtctcttg ctaaattttc ctccgtatca gcatcaagta ttccacaaac attacgagca aattctatta cagcaagctg caaaccaaga caaattccaa gaaagggaat attattctca cgagcatatt taatagccat aattttacct tcatatcctt tgcctccaaa gccgccagga acaataatgc cgtcaaactc ttttaaacag ctctcattta aatcattaga atcaattaaa gtgcttttaa taagcaaatc caaatgggct gcaacatgaa ccaaagactc tctaattgat gcataagaat catcaagttc agcatattta ccacaaatag caatattaat aattttttta ggcacaaaaa aattagattt tataactcct acaagctttg aaagctcttc tatttttgga tcaaccttaa tatttaactt agagcttaaa atctcatgta caccctgctt ataaaaagat ataggaattt cataaatagt agaaacatca acattgtcaa taatagaagt gctctcaaca ttgcaaaaca ttgccacttt ttttctgatt tggtctgtca atacttgtga acttctagca ataattaaat cggggaaaat acctgcttta tttaaggttt taacactttg ttgagtaggt ttagattttt gctcattaat tccagctgga cttggcacat atgttaaatg aataaaagaa atattaccac tcccaatctc ctgtcttatt tgtcttactg tctcaataaa taaaatattt tccatatctc ctacggttcc accaatttca attatcaaca tatcactatt ctcagaactt gcaatctgaa aaattgtaga tttgatctca tcagtaacat ggggaataag ctgaactgtt cttcccaaat atttaccctt tcgctcattt tcaagtatct ttttgtatat tttgcccatt gtaatgttcc aactagactt ggcattaaga tttaaaaacc tctcgtaatg accaaagtcc atatcaacct ctcctccatc atcaagcaca aaaacttctc cgtgctcaac aggattaata gtaccaggat cagtatttaa atacccatca catttaattg gagtaactct aaaatcatat ctaaacaacc ttgcaatact tgccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHAS IARLFRYDFR VTPIKCDGYL NTDPGTINPV EHGEVFVLDD GGEVDMDFGH YERFLNLNAK SSWNITMGKI YKKILENERK GKYLGRTVQL IPHVTDEIKS TIFQIASSEN SDMLIIEIGG TVGDMENILF IETVRQIRQE IGSGNISFIH LTYVPSPAGI NEQKSKPTQQ SVKTLNKAGI FPDLIIARSS QVLTDQIRKK VAMFCNVEST SIIDNVDVST IYEIPISFYK QGVHEILSSK LNIKVDPKIE ELSKLVGVIK SNFFVPKKII NIAICGKYAE LDDSYASIRE SLVHVAAHLD LLIKSTLIDS NDLNESCLKE FDGIIVPGGF GGKGYEGKIM AIKYARENNI PFLGICLGLQ LAVIEFARNV CGILDADTEE NLARDKPLKS PVIHLLPEQK GIKDKGATMR LGGYPVILKK NTIAFKLYGQ DRIIERFRHR YEVNNDYIDL FAKNGLIVSG FSSDFKMAKL IEIPENKFFV ACQFHPELIT RIENPAKLFL GLIKACI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcaagta ttgcaaggtt gtttagatat gattttagag ttactccaat taaatgtgat gggtatttaa atactgatcc tggtactatt aatcctgttg agcacggaga agtttttgtg cttgatgatg gaggagaggt tgatatggac tttggtcatt acgagaggtt tttaaatctt aatgccaagt ctagttggaa cattacaatg ggcaaaatat acaaaaagat acttgaaaat gagcgaaagg gtaaatattt gggaagaaca gttcagctta ttccccatgt tactgatgag atcaaatcta caatttttca gattgcaagt tctgagaata gtgatatgtt gataattgaa attggtggaa ccgtaggaga tatggaaaat attttattta ttgagacagt aagacaaata agacaggaga ttgggagtgg taatatttct tttattcatt taacatatgt gccaagtcca gctggaatta atgagcaaaa atctaaacct actcaacaaa gtgttaaaac cttaaataaa gcaggtattt tccccgattt aattattgct agaagttcac aagtattgac agaccaaatc agaaaaaaag tggcaatgtt ttgcaatgtt gagagcactt ctattattga caatgttgat gtttctacta tttatgaaat tcctatatct ttttataagc agggtgtaca tgagatttta agctctaagt taaatattaa ggttgatcca aaaatagaag agctttcaaa gcttgtagga gttataaaat ctaatttttt tgtgcctaaa aaaattatta atattgctat ttgtggtaaa tatgctgaac ttgatgattc ttatgcatca attagagagt ctttggttca tgttgcagcc catttggatt tgcttattaa aagcacttta attgattcta atgatttaaa tgagagctgt ttaaaagagt ttgacggcat tattgttcct ggcggctttg gaggcaaagg atatgaaggt aaaattatgg ctattaaata tgctcgtgag aataatattc cctttcttgg aatttgtctt ggtttgcagc ttgctgtaat agaatttgct cgtaatgttt gtggaatact tgatgctgat acggaggaaa atttagcaag agacaagccc ttaaaaagtc ctgttatcca tttacttcct gagcaaaagg gaattaaaga taagggcgct acaatgaggc ttggtggata tcctgtgatt cttaaaaaga atacaatagc ttttaaactt tatggccaag atcggataat tgaaagattt agacataggt atgaagtcaa taatgattat atagatttat ttgcaaaaaa tgggcttata gtatctggat tttcaagtga ttttaaaatg gcaaaattaa tagaaattcc tgaaaataaa tttttcgtag cttgccagtt tcatccagaa cttattacaa gaatagaaaa tccagccaag ctttttctag gattaattaa agcttgtatt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat