BobuA.17935.a

heat shock protein (groEL) (BB_0649)

CENTER ID: BobuA.17935.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17935.a.B1.GE39218 full length 1 545
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1040
RefSeq: NP_212783.1
UniProt: P0C923

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKDIYFNED ARKSLLSGVE KLSNAVKVTL GPKGRNVLID KKFGSPTVTK DGVSVAREIE LENPFENMGA QLLKEVAIKT NDVAGDGTTT ATVLAYAIAR EGLKNVSSGI NPIGIKKGID HAVNLAAEKI RQSAKKITTK EEIAQVASIS ANNDSYIGEK IAEAMDKVGK DGVITVEESK TFDTTISYVE GMQFDRGYLS PYFSTNKENM SVNFDDAFIL IYEKKISSIK ELLPVLEKVL GTNKPLLIIA EDIEGDALAA LVLNSVRGAL KVCAIKSPGF GDRRKAMLED IAVLTGGVLI SEELGLTLET VEIEQLGQAK TIKVDKDNTT IINTGNKEQI KERSELIKKQ IEDSTSEYDK EKLQERLAKL VGGVAVINVG AVTEVELKEK KHRVEDALSA TRAAVEEGVV PGGGSTLIEV AMYLDTIDTS KLSYEEKQGF EIVKRSLEEP MRQIISNAGF EGSIYIHQIK TEKKGLGFDA SSFKWVNMIE SGIIDPAKVT RSALQNAASI AGLLLTTECA ITDIKEEKNT SGGGGYPMDP GMGMM
NT Sequence
atggctaaag acatatattt taatgaggat gctagaaaaa gcttacttag tggcgttgaa aaattatcca atgctgtaaa agtaactctt gggccaaaag ggagaaatgt ccttattgat aaaaagttcg gctctccaac ggttacaaag gatggggtta gcgttgctcg tgagattgag cttgaaaatc cgtttgaaaa catgggggca cagcttttaa aggaagttgc tattaaaaca aatgatgttg ctggtgatgg aacaacaact gctactgttc ttgcttatgc tattgcaaga gaaggcctta agaatgtgtc ttcaggaatc aatcctattg gaataaaaaa gggaatagat cacgctgtaa atttggctgc tgagaaaatt cgtcagtctg caaaaaagat tacaacaaaa gaagagattg cacaagtagc ttcaatttct gctaataatg acagttatat aggtgaaaaa attgctgagg caatggataa agttggaaaa gatggtgtta taacagttga agagtcaaaa acctttgata ctacgatttc ttatgttgag ggtatgcaat ttgatagagg atatctttct ccttattttt ctaccaataa agaaaatatg agtgttaatt ttgacgatgc tttcatattg atatatgaga aaaagattag ttctattaaa gagcttttac cagttcttga gaaagtttta gggacaaata aacctttatt aattattgct gaggatattg agggggatgc tcttgctgct cttgttttaa acagcgttag aggagcttta aaggtatgtg caattaaatc tcctggtttt ggtgatagac gaaaagcaat gcttgaggat attgcagtgc ttaccggcgg tgttttaatc agtgaggagc taggccttac tcttgagaca gttgaaattg agcaacttgg acaggctaaa actattaagg ttgataaaga caataccact attattaata ccggcaataa agagcaaata aaggagcgtt cagagcttat taaaaagcaa attgaagatt caacatctga atatgataaa gaaaaacttc aagagcgtct tgcaaaactt gttggcggag ttgctgttat taatgttgga gctgttactg aggtagagct taaggagaaa aagcatagag ttgaggacgc tctttctgca actcgtgctg ctgttgaaga gggtgttgtg cctggcggtg gatcaactct tattgaagtt gctatgtatt tagatacaat agatacaagt aaattaagct atgaggaaaa gcaaggtttt gagattgtaa aaagaagtct tgaagagcca atgagacaga ttatttcaaa tgctggtttt gaaggatcta tttatattca tcaaattaaa acagaaaaaa aagggcttgg gtttgatgct tccagcttta agtgggtaaa tatgattgag agtggaataa ttgatcctgc taaggttaca agaagcgcgc ttcaaaatgc tgcttcaatt gctggacttt tattaacaac agaatgtgca atcacagata ttaaagaaga gaaaaatact tctggtggtg gtggttatcc tatggaccca ggaatgggaa tgatgtaa
Details for BobuA.17935.a.B1.GE39218
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 60kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA KDIYFNEDAR KSLLSGVEKL SNAVKVTLGP KGRNVLIDKK FGSPTVTKDG VSVAREIELE NPFENMGAQL LKEVAIKTND VAGDGTTTAT VLAYAIAREG LKNVSSGINP IGIKKGIDHA VNLAAEKIRQ SAKKITTKEE IAQVASISAN NDSYIGEKIA EAMDKVGKDG VITVEESKTF DTTISYVEGM QFDRGYLSPY FSTNKENMSV NFDDAFILIY EKKISSIKEL LPVLEKXLGT NKPLLIIAED IEGDALAALV LNSVRGALKV CAIKSPGFGD RRKAMLEDIA VLTGGVLISE ELGLTLETVE IEQLGQAKTI KVDKDNTTII NTGNKEQIKE RSELIKKQIE DSTSEYDKEK LQERLAKLVG GVAVINVGAV TEVELKEKKH RVEDALSATR AAVEEGVVPG GGSTLIEVAM YLDTIDTSKL SYEEKQGFGI VKRSLEEPMR QIISNAGFEG SIYIHQIKTE KKGLGFDASS FKWVNMIESG IIDPAKVTRS ALQNAASIAG LLLTTECAIT DIKEEKNTSG GGGY
Validated NT Sequence
gataaccacc accaccagaa gtatttttct cttctttaat atctgtgatt gcacattctg ttgttaataa aagtccagca attgaagcag cattttgaag cgcgcttctt gtaaccttag caggatcaat tattccactc tcaatcatat ttacccactt aaagctggaa gcatcaaacc caagcccttt tttttctgtt ttaatttgat gaatataaat agatccttca aaaccagcat ttgaaataat ctgtctcatt ggctcttcaa gacttctttt tacaatccca aaaccttgct tttcctcata gcttaattta cttgtatcta ttgtatctaa atacatagcg acttcaataa gagttgatcc accgccaggc acaacaccct cttcaacagc agcacgagtt gcagaaagag cgtcctcaac tctatgcttt ttctccttaa gctctacctc agtaacagct ccaacattaa taacagcaac tccgccaaca agttttgcaa gacgctcttg aagtttttct ttatcatatt cagatgttga atcttcaatt tgctttttaa taagctctga acgctccttt atttgctctt tattgccggt attaataata gtggtattgt ctttatcaac cttaatagtt ttagcctgtc caagttgctc aatttcaact gtctcaagag taaggcctag ctcctcactg attaaaacac cgccggtaag cactgcaata tcctcaagca ttgcttttcg tctatcacca aaaccaggag atttaattgc acataccttt aaagctcctc taacgctgtt taaaacaaga gcagcaagag catccccctc aatatcctca gcaataatta ataaaggttt atttgtccct aaanntttct caagaactgg taaaagctct ttaatagaac taatcttttt ctcatatatc aatatgaaag catcgtcaaa attaacactc atattttctt tattggtaga aaaataagga gaaagatatc ctctatcaaa ttgcataccc tcaacataag aaatcgtagt atcaaaggtt tttgactctt caactgttat aacaccatct tttccaactt tatccattgc ctcagcaatt ttttcaccta tataactgtc attattagca gaaattgaag ctacttgtgc aatctcttct tttgttgtaa tcttttttgc agactgacga attttctcag cagccaaatt tacagcgtga tctattccct tttttattcc aataggattg attcctgaag acacattctt aaggccttct cttgcaatag cataagcaag aacagtagca gttgttgttc catcaccagc aacatcattt gttttaatag caacttcctt taaaagctgt gcccccatgt tttcaaacgg attttcaagc tcaatctcac gagcaacgct aaccccatcc tttgtaaccg ttggagagcc gaacttttta tcaataagga catttctccc ttttggccca agagttactt ttacagcatt ggataatttt tcaacgccac taagtaagct ttttctagca tcctcattaa aatatatgtc tttagccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMA KDIYFNEDAR KSLLSGVEKL SNAVKVTLGP KGRNVLIDKK FGSPTVTKDG VSVAREIELE NPFENMGAQL LKEVAIKTND VAGDGTTTAT VLAYAIAREG LKNVSSGINP IGIKKGIDHA VNLAAEKIRQ SAKKITTKEE IAQVASISAN NDSYIGEKIA EAMDKVGKDG VITVEESKTF DTTISYVEGM QFDRGYLSPY FSTNKENMSV NFDDAFILIY EKKISSIKEL LPVLEKVLGT NKPLLIIAED IEGDALAALV LNSVRGALKV CAIKSPGFGD RRKAMLEDIA VLTGGVLISE ELGLTLETVE IEQLGQAKTI KVDKDNTTII NTGNKEQIKE RSELIKKQIE DSTSEYDKEK LQERLAKLVG GVAVINVGAV TEVELKEKKH RVEDALSATR AAVEEGVVPG GGSTLIEVAM YLDTIDTSKL SYEEKQGFEI VKRSLEEPMR QIISNAGFEG SIYIHQIKTE KKGLGFDASS FKWVNMIESG IIDPAKVTRS ALQNAASIAG LLLTTECAIT DIKEEKNTSG GGGYPMDPGM GMM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta aagacatata ttttaatgag gatgctagaa aaagcttact tagtggcgtt gaaaaattat ccaatgctgt aaaagtaact cttgggccaa aagggagaaa tgtccttatt gataaaaagt tcggctctcc aacggttaca aaggatgggg ttagcgttgc tcgtgagatt gagcttgaaa atccgtttga aaacatgggg gcacagcttt taaaggaagt tgctattaaa acaaatgatg ttgctggtga tggaacaaca actgctactg ttcttgctta tgctattgca agagaaggcc ttaagaatgt gtcttcagga atcaatccta ttggaataaa aaagggaata gatcacgctg taaatttggc tgctgagaaa attcgtcagt ctgcaaaaaa gattacaaca aaagaagaga ttgcacaagt agcttcaatt tctgctaata atgacagtta tataggtgaa aaaattgctg aggcaatgga taaagttgga aaagatggtg ttataacagt tgaagagtca aaaacctttg atactacgat ttcttatgtt gagggtatgc aatttgatag aggatatctt tctccttatt tttctaccaa taaagaaaat atgagtgtta attttgacga tgctttcata ttgatatatg agaaaaagat tagttctatt aaagagcttt taccagttct tgagaaagtt ttagggacaa ataaaccttt attaattatt gctgaggata ttgaggggga tgctcttgct gctcttgttt taaacagcgt tagaggagct ttaaaggtat gtgcaattaa atctcctggt tttggtgata gacgaaaagc aatgcttgag gatattgcag tgcttaccgg cggtgtttta atcagtgagg agctaggcct tactcttgag acagttgaaa ttgagcaact tggacaggct aaaactatta aggttgataa agacaatacc actattatta ataccggcaa taaagagcaa ataaaggagc gttcagagct tattaaaaag caaattgaag attcaacatc tgaatatgat aaagaaaaac ttcaagagcg tcttgcaaaa cttgttggcg gagttgctgt tattaatgtt ggagctgtta ctgaggtaga gcttaaggag aaaaagcata gagttgagga cgctctttct gcaactcgtg ctgctgttga agagggtgtt gtgcctggcg gtggatcaac tcttattgaa gttgctatgt atttagatac aatagataca agtaaattaa gctatgagga aaagcaaggt tttgagattg taaaaagaag tcttgaagag ccaatgagac agattatttc aaatgctggt tttgaaggat ctatttatat tcatcaaatt aaaacagaaa aaaaagggct tgggtttgat gcttccagct ttaagtgggt aaatatgatt gagagtggaa taattgatcc tgctaaggtt acaagaagcg cgcttcaaaa tgctgcttca attgctggac ttttattaac aacagaatgt gcaatcacag atattaaaga agagaaaaat acttctggtg gtggtggtta tcctatggac ccaggaatgg gaatgatgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat