BobuA.17897.a

ATP-dependent zinc metalloprotease FtsH (EC 3.4.24.-); cell division protein (BB_0789)

CENTER ID: BobuA.17897.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17897.a.B2.GE39213 soluble domain 142 639
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1181
RefSeq: NP_212923.1
UniProt: O51729

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNGNNNMNNN GKSNNKKKNK NWILGLVVVF LISAIFMSYF IRGGESYKNV PYSTFQSYLD NGLVESVVII DKNLIQFVVK GSNFAKSYFS TSIPYLDINL LSELKNKKVE LSSGKSQASL IGVLLQTLPW ILFFIFFFFI FRQTQGGGGK VFTFGKSNAQ KYEAGKNKIT FKDVAGQEEV KQELREVVEF LKNPKKFEKI GAKIPKGVLL VGSPGTGKTL LAKAVAGEAG VSFFHMSGSD FVEMFVGVGA SRVRDLFDNA RKNSPCIIFI DELDAVGRSR GAGLGGGHDE REQTLNQLLV EMDGFGTHTN VIVMAATNRP DVLDSALLRP GRFDRQVTVS LPDIKEREAI LNIHSLKTKL SKDINLQVIA RATPGASGAD LANLINEGAL IAARNNQDEI LMKDMEEARD KILMGVAKKS MTITDRQKLE TAYHEAGHAL LHYYLEHADP LHKVTIIPRG RALGVAFSLP REDRLSINKH QILDKIKICY GGYASEQINL GVTTAGVQND LMQATSLAKK MVTEWGMGEE VGPIFLVDDE APIFLPKEFS KAKAYSENTA DKVDREVKRI LEECLKEASD ILLKHKDQLV KLAKELVLKE TLTDKEVREL LGFEANKDEY DLFSSDSTTK EVKGEDVKG
NT Sequence
atgaatggca ataataatat gaataacaat ggaaaatcta acaacaaaaa aaagaataaa aattggattt taggacttgt tgttgttttt ctaatttcag caatatttat gtcatatttt ataagggggg gggaaagcta taaaaatgtt ccttatagca cttttcagag ttatttagac aatggtctag ttgagtctgt agtaataatt gataaaaatt tgattcaatt tgtcgtcaag ggttctaact ttgcaaagtc ttatttttct accagcattc cctatcttga tataaattta ctttcagaac taaaaaataa aaaagttgag cttagctcag ggaaaagtca agcttctctg attggggttt tattacaaac tttgccatgg attttgtttt ttattttctt tttctttata tttcgtcaaa ctcaaggtgg cggtgggaag gtttttacat ttgggaaaag taatgctcaa aagtatgaag ctggaaagaa taaaatcacc tttaaagatg tggctggtca agaagaggtt aagcaagagc ttcgagaagt tgtcgaattt cttaaaaatc caaaaaaatt tgaaaaaata ggtgcaaaaa tccccaaagg agtgctttta gtgggctctc cgggtactgg caagactttg cttgccaaag ccgttgccgg tgaggctggg gttagtttct ttcacatgtc aggctcagat tttgttgaaa tgtttgttgg ggttggagca agtcgtgtta gagatttatt tgataatgct agaaaaaatt ctccatgtat tatttttatt gatgagcttg atgctgttgg tcgaagtcgt ggtgcggggc ttggcggtgg tcatgatgaa agagagcaaa ctcttaatca gctgttagtt gaaatggatg gatttggaac gcataccaat gtaattgtta tggccgctac aaatcgtccc gatgttcttg actctgcttt gcttaggcct ggacgatttg acaggcaagt aacagtttct ttgcctgata ttaaggaaag agaggcaata ttaaatattc attctttaaa aacgaagctt tcaaaagata ttaatttaca agtaatagca agagctactc ctggagctag tggtgctgat cttgcaaatt taattaatga aggagcctta atagctgcaa ggaataatca agatgaaatt ttaatgaagg atatggaaga agccagagat aaaatattga tgggagttgc aaaaaaatcc atgactatta ctgataggca aaagcttgag acggcttatc atgaggcagg ccatgctttg cttcactatt atcttgaaca tgctgatcca cttcacaaag ttaccattat tccaaggggc agggcacttg gtgttgcatt ttctcttcca agggaagata gactttcaat aaacaaacac caaattcttg acaaaataaa gatatgctat ggtggttatg ctagcgagca aataaatttg ggtgttacaa cagcgggtgt tcaaaatgat ttaatgcaag ctactagttt ggctaaaaaa atggttacgg agtggggaat gggtgaagaa gttggtccaa tatttttagt agacgatgaa gcacctattt tccttccgaa agagttttca aaggctaaag cttattctga gaacactgcc gataaagttg acagagaggt aaaaagaata cttgaagaat gtttaaaaga ggcatcggat attcttttaa agcataagga tcaacttgtt aagcttgcaa aagaacttgt tttaaaagaa actttgacag acaaagaagt gagagagctt ttgggttttg aagctaataa ggatgaatac gatttatttt catcagactc tactacaaaa gaagtaaagg gggaggatgt aaaaggttag
Details for BobuA.17897.a.B2.GE39213
HARVESTED ON: data unavailable
SEQUENCED ON: 6/5/2015
EXPECTED MW: 56kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: None
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHRQ TQGGGGKVFT FGKSNAQKYE AGKNKITFKD VAGQEEVKQE LREVVEFLKN PKKFEKIGAK IPKGVLLVGS PGTGKTLLAK AVAGEAGVSF FHMSGSDFVE MFVGVGASRV RDLFDNARKN SPCIIFIDEL DAVGRSRGAG LGGGHDEREQ TLNQLLVEMD GFGTHTNVIV MAATNRPDVL DSALLRPGRF DRQVTVSLPD IKEREAILNI HSLKTKLSKD INLQVIARAT PGASGADLAN LINEGALIAA RNNQDEILMK DMEEARDKIL MGVAKKSMTI TDRQKLETAY HEAGHALLHY YLEHADPLHK VTIIPRGRAL GVAFSLPRED RLSINKHQIL DKIKICYGGY ASEQINLGVT TAGVQNDLMQ ATSLAKKMVT EWGMGEEVGP IFLVDDEAPI FLPKEFSKAK AYSENTADKV DREVKRILEE CLKEASDILL KHKDQLVKLA KELVLKETLT DKEVRELLGF EANKDEYDLF SSDSTTKEVK GEDVKG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgtcaaa ctcaaggtgg cggtgggaag gtttttacat ttgggaaaag taatgctcaa aagtatgaag ctggaaagaa taaaatcacc tttaaagatg tggctggtca agaagaggtt aagcaagagc ttcgagaagt tgtcgaattt cttaaaaatc caaaaaaatt tgaaaaaata ggtgcaaaaa tccccaaagg agtgctttta gtgggctctc cgggtactgg caagactttg cttgccaaag ccgttgccgg tgaggctggg gttagtttct ttcacatgtc aggctcagat tttgttgaaa tgtttgttgg ggttggagca agtcgtgtta gagatttatt tgataatgct agaaaaaatt ctccatgtat tatttttatt gatgagcttg atgctgttgg tcgaagtcgt ggtgcggggc ttggcggtgg tcatgatgaa agagagcaaa ctcttaatca gctgttagtt gaaatggatg gatttggaac gcataccaat gtaattgtta tggccgctac aaatcgtccc gatgttcttg actctgcttt gcttaggcct ggacgatttg acaggcaagt aacagtttct ttgcctgata ttaaggaaag agaggcaata ttaaatattc attctttaaa aacgaagctt tcaaaagata ttaatttaca agtaatagca agagctactc ctggagctag tggtgctgat cttgcaaatt taattaatga aggagcctta atagctgcaa ggaataatca agatgaaatt ttaatgaagg atatggaaga agccagagat aaaatattga tgggagttgc aaaaaaatcc atgactatta ctgataggca aaagcttgag acggcttatc atgaggcagg ccatgctttg cttcactatt atcttgaaca tgctgatcca cttcacaaag ttaccattat tccaaggggc agggcacttg gtgttgcatt ttctcttcca agggaagata gactttcaat aaacaaacac caaattcttg acaaaataaa gatatgctat ggtggttatg ctagcgagca aataaatttg ggtgttacaa cagcgggtgt tcaaaatgat ttaatgcaag ctactagttt ggctaaaaaa atggttacgg agtggggaat gggtgaagaa gttggtccaa tatttttagt agacgatgaa gcacctattt tccttccgaa agagttttca aaggctaaag cttattctga gaacactgcc gataaagttg acagagaggt aaaaagaata cttgaagaat gtttaaaaga ggcatcggat attcttttaa agcataagga tcaacttgtt aagcttgcaa aagaacttgt tttaaaagaa actttgacag acaaagaagt gagagagctt ttgggttttg aagctaataa ggatgaatac gatttatttt catcagactc tactacaaaa gaagtaaagg gggaggatgt aaaaggttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at