BobuA.17876.a

GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2) (GMP synthetase) (Glutamine amidotransferase) (BB_B18)

CENTER ID: BobuA.17876.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17876.a.B1.GE38699 full length 1 511
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17876.a.B1.PS02400 full length 1 511
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|224326.49.peg.1606
RefSeq: NP_047004.2
RefSeq: WP_010890581.1
UniProt: P0CL64
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTQAILVLD FGSQYSQLIA RRIREIGVYT KVIPYYTPLK EIKNMNISGI ILSGSPASVY SKEAPTLNME IFNLKIPILG ICYGMQIIVK LFGGLVSKDS KQEYGSSEIF LRDEKSLLFS ELPNKFQIIM SHGDSIEKIP DNFKQLAFTK NCIASISNET QKIYGLQFHP EVTHSEFGDQ IIKNFVFKIC QAQINWSLEG NLETIVKKIK LKVGSKKVIL GLSGGTDSLV CALLIKKAIN ENLICVFVNT GLLRKNEDKK ILELKHQYDL NIKYIDASTK FLNRLKNISD PEEKRKIIGK EFVDVFEKIT LEDQNIEYLA QGTIYSDVIE SKSKDSSSSK IKSHHNVGGL PDKMSLKLLE PLNEFFKDEI IQIGINLGIK KESLYRHPFP GPGLAIRIIG EVTQEKINIL QEADNILTEE LFINDLYYQI RQAFVVLLPV KSVGVMGDQR TYEYTAVIRC VNTQDFMTAE WTELPYSFLK KVSSRIINEV RGINRVCYDI SSKPPSTIEW E
NT Sequence
atgaatactc aggcaatact tgtattagat tttggatccc aatatagcca actaattgca agaagaatta gagaaattgg cgtttataca aaagtaatac cttactatac tcctttaaaa gaaattaaaa atatgaatat ctcaggaata atactaagtg gaagtcctgc ttctgtttat tcaaaagaag ctcctacctt gaatatggaa atttttaatt tgaaaatacc tattttgggt atatgttatg gaatgcaaat aattgttaaa ttatttgggg gcctagtatc taaagactct aagcaagaat atgggagctc tgaaatcttt ctaagagatg aaaaatctct tttattttca gaacttccaa acaaatttca aattatcatg agtcatggag acagtattga aaaaattcct gataatttca aacaattagc ttttacaaaa aattgtattg cttctatatc aaatgaaact caaaaaattt acggcctaca attccatcca gaagtaactc attctgaatt tggtgatcaa ataattaaaa attttgtttt taaaatttgc caagcccaaa ttaattggtc attagaaggc aatctagaaa ccattgtgaa aaaaattaag cttaaagtgg gaagcaaaaa ggttatttta ggactttctg gtggcacaga ctctttagtt tgcgcattgc ttataaaaaa agctataaac gaaaatttga tctgcgtttt tgtaaacact ggattgttgc gcaaaaatga agataaaaaa atactagaat taaagcatca atatgattta aatataaaat atattgatgc ttctacaaaa ttcttgaacc gtttaaaaaa tataagtgat cctgaggaaa agagaaaaat aataggaaaa gaatttgtag atgtttttga aaaaattact ctagaagatc aaaatataga atatttagct caaggaacaa tttattctga cgtaattgaa tctaaatcaa aagacagctc ttcttcaaaa atcaagtctc atcacaacgt agggggactc ccagataaga tgagtttaaa actcttagaa cctttgaatg aattttttaa agatgaaata attcaaatcg gaataaatct aggtattaaa aaagaatctc tttacagaca tccatttccc ggcccaggac tagctataag aataattgga gaagtaacac aagaaaagat aaatatctta caagaagcag acaatattct aacagaggag ctttttataa atgatttata ttatcaaata agacaagcat ttgttgtatt gcttcctgtc aaatctgtag gcgtaatggg agatcaaagg acatacgaat atacagctgt aattagatgt gtaaataccc aagacttcat gactgcagaa tggactgaac ttccttacag ttttttaaaa aaagtttctt caagaataat taatgaagtt cggggtataa atagagtttg ttatgatata tcttctaagc ctccatcaac catagaatgg gaataa
Details for BobuA.17876.a.B1.GE38699
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN TQAILVLDFG SQYSQLIARR IREIGVYTKV IPYYTPLKEI KNMNISGIIL SGSPASVYSK EAPTLNMEIF NLKIPILGIC YGMQIIVKLF GGLVSKDSKQ EYGSSEIFLR DEKSLLFSEL PNKFQIIMSH GDSIEKIPDN FKQLAFTKNC IASISNETQK IYGLQFHPEV THSEFGDQII KNFVFKICQA QINWSLEGNL ETIVKKIKLK VGSKKVILGL SGGTDSLVCA LLIKKAINEN LICVFVNTGL LRKNEDKKIL ELKHQYDLNI KYIDASTKFL NRLKNISDPE EKRKIIGKEF VDVFEKITLE DQNIEYLAQG TIYSDVIESK SKDSSSSKIK SHHNVGGLPD KMSLKLLEPL NEFFKDEIIQ IGINLGIKKE SLYRHPFPGP GLAIRIIGEV TQEKINILQE ADNILTEELF INDLYYQIRQ AFVVLLPVKS VGVMGDQRTY EYTAVIRCVN TQDFMTAEWT ELPYSFLKKV SSRIINEVRG INRVCYDISS K
Validated NT Sequence
ggcttagaag atatatcata acaaactcta tttatacccc gaacttcatt aattattctt gaagaaactt tttttaaaaa actgtaagga agttcagtcc attctgcagt catgaagtct tgggtattta cacatctaat tacagctgta tattcgtatg tcctttgatc tcccattacg cctacagatt tgacaggaag caatacaaca aatgcttgtc ttatttgata atataaatca tttataaaaa gctcctctgt tagaatattg tctgcttctt gtaagatatt tatcttttct tgtgttactt ctccaattat tcttatagct agtcctgggc cgggaaatgg atgtctgtaa agagattctt ttttaatacc tagatttatt ccgatttgaa ttatttcatc tttaaaaaat tcattcaaag gttctaagag ttttaaactc atcttatctg ggagtccccc tacgttgtga tgagacttga tttttgaaga agagctgtct tttgatttag attcaattac gtcagaataa attgttcctt gagctaaata ttctatattt tgatcttcta gagtaatttt ttcaaaaaca tctacaaatt cttttcctat tatttttctc ttttcctcag gatcacttat attttttaaa cggttcaaga attttgtaga agcatcaata tattttatat ttaaatcata ttgatgcttt aattctagta tttttttatc ttcatttttg cgcaacaatc cagtgtttac aaaaacgcag atcaaatttt cgtttatagc tttttttata agcaatgcgc aaactaaaga gtctgtgcca ccagaaagtc ctaaaataac ctttttgctt cccactttaa gcttaatttt tttcacaatg gtttctagat tgccttctaa tgaccaatta atttgggctt ggcaaatttt aaaaacaaaa tttttaatta tttgatcacc aaattcagaa tgagttactt ctggatggaa ttgtaggccg taaatttttt gagtttcatt tgatatagaa gcaatacaat tttttgtaaa agctaattgt ttgaaattat caggaatttt ttcaatactg tctccatgac tcatgataat ttgaaatttg tttggaagtt ctgaaaataa aagagatttt tcatctctta gaaagatttc agagctccca tattcttgct tagagtcttt agatactagg cccccaaata atttaacaat tatttgcatt ccataacata tacccaaaat aggtattttc aaattaaaaa tttccatatt caaggtagga gcttcttttg aataaacaga agcaggactt ccacttagta ttattcctga gatattcata tttttaattt cttttaaagg agtatagtaa ggtattactt ttgtataaac gccaatttct ctaattcttc ttgcaattag ttggctatat tgggatccaa aatctaatac aagtattgcc tgagtattca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMN TQAILVLDFG SQYSQLIARR IREIGVYTKV IPYYTPLKEI KNMNISGIIL SGSPASVYSK EAPTLNMEIF NLKIPILGIC YGMQIIVKLF GGLVSKDSKQ EYGSSEIFLR DEKSLLFSEL PNKFQIIMSH GDSIEKIPDN FKQLAFTKNC IASISNETQK IYGLQFHPEV THSEFGDQII KNFVFKICQA QINWSLEGNL ETIVKKIKLK VGSKKVILGL SGGTDSLVCA LLIKKAINEN LICVFVNTGL LRKNEDKKIL ELKHQYDLNI KYIDASTKFL NRLKNISDPE EKRKIIGKEF VDVFEKITLE DQNIEYLAQG TIYSDVIESK SKDSSSSKIK SHHNVGGLPD KMSLKLLEPL NEFFKDEIIQ IGINLGIKKE SLYRHPFPGP GLAIRIIGEV TQEKINILQE ADNILTEELF INDLYYQIRQ AFVVLLPVKS VGVMGDQRTY EYTAVIRCVN TQDFMTAEWT ELPYSFLKKV SSRIINEVRG INRVCYDISS KPPSTIEWE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata ctcaggcaat acttgtatta gattttggat cccaatatag ccaactaatt gcaagaagaa ttagagaaat tggcgtttat acaaaagtaa taccttacta tactccttta aaagaaatta aaaatatgaa tatctcagga ataatactaa gtggaagtcc tgcttctgtt tattcaaaag aagctcctac cttgaatatg gaaattttta atttgaaaat acctattttg ggtatatgtt atggaatgca aataattgtt aaattatttg ggggcctagt atctaaagac tctaagcaag aatatgggag ctctgaaatc tttctaagag atgaaaaatc tcttttattt tcagaacttc caaacaaatt tcaaattatc atgagtcatg gagacagtat tgaaaaaatt cctgataatt tcaaacaatt agcttttaca aaaaattgta ttgcttctat atcaaatgaa actcaaaaaa tttacggcct acaattccat ccagaagtaa ctcattctga atttggtgat caaataatta aaaattttgt ttttaaaatt tgccaagccc aaattaattg gtcattagaa ggcaatctag aaaccattgt gaaaaaaatt aagcttaaag tgggaagcaa aaaggttatt ttaggacttt ctggtggcac agactcttta gtttgcgcat tgcttataaa aaaagctata aacgaaaatt tgatctgcgt ttttgtaaac actggattgt tgcgcaaaaa tgaagataaa aaaatactag aattaaagca tcaatatgat ttaaatataa aatatattga tgcttctaca aaattcttga accgtttaaa aaatataagt gatcctgagg aaaagagaaa aataatagga aaagaatttg tagatgtttt tgaaaaaatt actctagaag atcaaaatat agaatattta gctcaaggaa caatttattc tgacgtaatt gaatctaaat caaaagacag ctcttcttca aaaatcaagt ctcatcacaa cgtaggggga ctcccagata agatgagttt aaaactctta gaacctttga atgaattttt taaagatgaa ataattcaaa tcggaataaa tctaggtatt aaaaaagaat ctctttacag acatccattt cccggcccag gactagctat aagaataatt ggagaagtaa cacaagaaaa gataaatatc ttacaagaag cagacaatat tctaacagag gagcttttta taaatgattt atattatcaa ataagacaag catttgttgt attgcttcct gtcaaatctg taggcgtaat gggagatcaa aggacatacg aatatacagc tgtaattaga tgtgtaaata cccaagactt catgactgca gaatggactg aacttcctta cagtttttta aaaaaagttt cttcaagaat aattaatgaa gttcggggta taaatagagt ttgttatgat atatcttcta agcctccatc aaccatagaa tgggaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BobuA.17876.a.B1.PS02400
PURIFICATION DATe: 5/14/2015
CONCENTRATION: 52.6mg/ml
OBSERVED MW: 62kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN TQAILVLDFG SQYSQLIARR IREIGVYTKV IPYYTPLKEI KNMNISGIIL SGSPASVYSK EAPTLNMEIF NLKIPILGIC YGMQIIVKLF GGLVSKDSKQ EYGSSEIFLR DEKSLLFSEL PNKFQIIMSH GDSIEKIPDN FKQLAFTKNC IASISNETQK IYGLQFHPEV THSEFGDQII KNFVFKICQA QINWSLEGNL ETIVKKIKLK VGSKKVILGL SGGTDSLVCA LLIKKAINEN LICVFVNTGL LRKNEDKKIL ELKHQYDLNI KYIDASTKFL NRLKNISDPE EKRKIIGKEF VDVFEKITLE DQNIEYLAQG TIYSDVIESK SKDSSSSKIK SHHNVGGLPD KMSLKLLEPL NEFFKDEIIQ IGINLGIKKE SLYRHPFPGP GLAIRIIGEV TQEKINILQE ADNILTEELF INDLYYQIRQ AFVVLLPVKS VGVMGDQRTY EYTAVIRCVN TQDFMTAEWT ELPYSFLKKV SSRIINEVRG INRVCYDISS K
Validated NT Sequence
ggcttagaag atatatcata acaaactcta tttatacccc gaacttcatt aattattctt gaagaaactt tttttaaaaa actgtaagga agttcagtcc attctgcagt catgaagtct tgggtattta cacatctaat tacagctgta tattcgtatg tcctttgatc tcccattacg cctacagatt tgacaggaag caatacaaca aatgcttgtc ttatttgata atataaatca tttataaaaa gctcctctgt tagaatattg tctgcttctt gtaagatatt tatcttttct tgtgttactt ctccaattat tcttatagct agtcctgggc cgggaaatgg atgtctgtaa agagattctt ttttaatacc tagatttatt ccgatttgaa ttatttcatc tttaaaaaat tcattcaaag gttctaagag ttttaaactc atcttatctg ggagtccccc tacgttgtga tgagacttga tttttgaaga agagctgtct tttgatttag attcaattac gtcagaataa attgttcctt gagctaaata ttctatattt tgatcttcta gagtaatttt ttcaaaaaca tctacaaatt cttttcctat tatttttctc ttttcctcag gatcacttat attttttaaa cggttcaaga attttgtaga agcatcaata tattttatat ttaaatcata ttgatgcttt aattctagta tttttttatc ttcatttttg cgcaacaatc cagtgtttac aaaaacgcag atcaaatttt cgtttatagc tttttttata agcaatgcgc aaactaaaga gtctgtgcca ccagaaagtc ctaaaataac ctttttgctt cccactttaa gcttaatttt tttcacaatg gtttctagat tgccttctaa tgaccaatta atttgggctt ggcaaatttt aaaaacaaaa tttttaatta tttgatcacc aaattcagaa tgagttactt ctggatggaa ttgtaggccg taaatttttt gagtttcatt tgatatagaa gcaatacaat tttttgtaaa agctaattgt ttgaaattat caggaatttt ttcaatactg tctccatgac tcatgataat ttgaaatttg tttggaagtt ctgaaaataa aagagatttt tcatctctta gaaagatttc agagctccca tattcttgct tagagtcttt agatactagg cccccaaata atttaacaat tatttgcatt ccataacata tacccaaaat aggtattttc aaattaaaaa tttccatatt caaggtagga gcttcttttg aataaacaga agcaggactt ccacttagta ttattcctga gatattcata tttttaattt cttttaaagg agtatagtaa ggtattactt ttgtataaac gccaatttct ctaattcttc ttgcaattag ttggctatat tgggatccaa aatctaatac aagtattgcc tgagtattca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMN TQAILVLDFG SQYSQLIARR IREIGVYTKV IPYYTPLKEI KNMNISGIIL SGSPASVYSK EAPTLNMEIF NLKIPILGIC YGMQIIVKLF GGLVSKDSKQ EYGSSEIFLR DEKSLLFSEL PNKFQIIMSH GDSIEKIPDN FKQLAFTKNC IASISNETQK IYGLQFHPEV THSEFGDQII KNFVFKICQA QINWSLEGNL ETIVKKIKLK VGSKKVILGL SGGTDSLVCA LLIKKAINEN LICVFVNTGL LRKNEDKKIL ELKHQYDLNI KYIDASTKFL NRLKNISDPE EKRKIIGKEF VDVFEKITLE DQNIEYLAQG TIYSDVIESK SKDSSSSKIK SHHNVGGLPD KMSLKLLEPL NEFFKDEIIQ IGINLGIKKE SLYRHPFPGP GLAIRIIGEV TQEKINILQE ADNILTEELF INDLYYQIRQ AFVVLLPVKS VGVMGDQRTY EYTAVIRCVN TQDFMTAEWT ELPYSFLKKV SSRIINEVRG INRVCYDISS KPPSTIEWE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA CTCAGGCAAT ACTTGTATTA GATTTTGGAT CCCAATATAG CCAACTAATT GCAAGAAGAA TTAGAGAAAT TGGCGTTTAT ACAAAAGTAA TACCTTACTA TACTCCTTTA AAAGAAATTA AAAATATGAA TATCTCAGGA ATAATACTAA GTGGAAGTCC TGCTTCTGTT TATTCAAAAG AAGCTCCTAC CTTGAATATG GAAATTTTTA ATTTGAAAAT ACCTATTTTG GGTATATGTT ATGGAATGCA AATAATTGTT AAATTATTTG GGGGCCTAGT ATCTAAAGAC TCTAAGCAAG AATATGGGAG CTCTGAAATC TTTCTAAGAG ATGAAAAATC TCTTTTATTT TCAGAACTTC CAAACAAATT TCAAATTATC ATGAGTCATG GAGACAGTAT TGAAAAAATT CCTGATAATT TCAAACAATT AGCTTTTACA AAAAATTGTA TTGCTTCTAT ATCAAATGAA ACTCAAAAAA TTTACGGCCT ACAATTCCAT CCAGAAGTAA CTCATTCTGA ATTTGGTGAT CAAATAATTA AAAATTTTGT TTTTAAAATT TGCCAAGCCC AAATTAATTG GTCATTAGAA GGCAATCTAG AAACCATTGT GAAAAAAATT AAGCTTAAAG TGGGAAGCAA AAAGGTTATT TTAGGACTTT CTGGTGGCAC AGACTCTTTA GTTTGCGCAT TGCTTATAAA AAAAGCTATA AACGAAAATT TGATCTGCGT TTTTGTAAAC ACTGGATTGT TGCGCAAAAA TGAAGATAAA AAAATACTAG AATTAAAGCA TCAATATGAT TTAAATATAA AATATATTGA TGCTTCTACA AAATTCTTGA ACCGTTTAAA AAATATAAGT GATCCTGAGG AAAAGAGAAA AATAATAGGA AAAGAATTTG TAGATGTTTT TGAAAAAATT ACTCTAGAAG ATCAAAATAT AGAATATTTA GCTCAAGGAA CAATTTATTC TGACGTAATT GAATCTAAAT CAAAAGACAG CTCTTCTTCA AAAATCAAGT CTCATCACAA CGTAGGGGGA CTCCCAGATA AGATGAGTTT AAAACTCTTA GAACCTTTGA ATGAATTTTT TAAAGATGAA ATAATTCAAA TCGGAATAAA TCTAGGTATT AAAAAAGAAT CTCTTTACAG ACATCCATTT CCCGGCCCAG GACTAGCTAT AAGAATAATT GGAGAAGTAA CACAAGAAAA GATAAATATC TTACAAGAAG CAGACAATAT TCTAACAGAG GAGCTTTTTA TAAATGATTT ATATTATCAA ATAAGACAAG CATTTGTTGT ATTGCTTCCT GTCAAATCTG TAGGCGTAAT GGGAGATCAA AGGACATACG AATATACAGC TGTAATTAGA TGTGTAAATA CCCAAGACTT CATGACTGCA GAATGGACTG AACTTCCTTA CAGTTTTTTA AAAAAAGTTT CTTCAAGAAT AATTAATGAA GTTCGGGGTA TAAATAGAGT TTGTTATGAT ATATCTTCTA AGCCTCCATC AACCATAGAA TGGGAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T