BobuA.17839.a

BB_C01

CENTER ID: BobuA.17839.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17839.a.B1.GE38634 full length 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.117
RefSeq: NP_051278.1
RefSeq: WP_010883782.1
UniProt: Q9S0E5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKDFLITTKN PTCHNKHQHK LIYLTSTVDF LNKKDKKYTQ QNILYYYNKN LKRNGLAPTT LRTMQNYLYK LEKVLKVTTN YYQHMGVNCG TEIYYKLKYP KKECYQKINK YFKERKNSRF KSRVNNHFKD NVSKNSSVNS VECLSNKNNI KEERKIKEIE KYQLRNYFNN CNFKTEEALS ILYLNTDKDT KIEAINILKQ NEIALIKKFN IKKSCMKEKQ KKLKEILYNT RKKLEENGYN PKQLEINLQK VYENYKYKPH FIIENHKYND LGYIKRKLEK SIERKKENSQ QNYQNLKENI FNILIEQLKK EVNIEILKPI IKEYLNNQKK IEYNKVFGIY HLELLEIIKN EKNSLTTEEF SIKAV
NT Sequence
atgaaagatt ttttaatcac cacaaaaaat cccacttgcc acaacaaaca ccaacacaag ttaatatatc ttacatcaac agtagatttt ctaaacaaaa aagataagaa atatacacaa caaaacatac tctattacta taataaaaat ctaaaaagaa atggtttggc tcccactaca ctgagaacaa tgcaaaatta tctttataaa ttagaaaaag tattaaaagt cacaacgaat tactaccaac acatgggtgt aaattgtgga actgaaattt actataagct aaagtatcct aaaaaagaat gttaccagaa aatcaacaag tactttaaag aacgaaaaaa ctctagattt aaatctagag ttaataacca ttttaaagac aatgtttcta aaaatagtag tgtaaattca gtggagtgtt taagtaataa aaataatata aaagaagaaa gaaagattaa agaaatagaa aaatatcaac taagaaatta tttcaataat tgtaacttta aaacggaaga agctctttct attttgtatt taaatactga taaagatact aagattgagg caataaatat cttaaaacaa aatgaaattg ccctaataaa aaaattcaat ataaaaaaat cttgcatgaa agaaaaacaa aaaaaattaa aagaaattct atacaacact aggaaaaaat tagaagaaaa cgggtacaat cccaaacaat tagaaataaa tttacaaaaa gtatacgaaa attacaaata caagccccat tttattattg aaaatcataa atataatgat ttaggttaca taaaacgtaa attagaaaag tcgattgaaa gaaaaaaaga aaattctcaa caaaattatc aaaatttaaa ggaaaatatt ttcaatatcc ttattgaaca actaaaaaaa gaagtaaata ttgaaattct aaagccaatt ataaaagaat atttgaataa ccaaaagaaa atagaataca ataaagtatt tggtatatat catcttgaat tattagaaat aataaaaaat gaaaaaaatt ctttaaccac agaagaattt agcataaagg ccgtatga
Details for BobuA.17839.a.B1.GE38634
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 45kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHHM KDFLITTKNP TCHNKHQHKL IYLTSTVDFL NKKDKKYTQQ NILYYYNKNL KRNGLAPTTL RTMQNYLYKL EKVLKVTTNY YQHMGVNCGT EIYYKLKYPK KECYQKINKY FKERKNSRFK SRVNNHFKDN VSKNSSVNSV ECLSNKNNIK EERKIKEIEK YQLRNYFNNC NFKTEEALSI LYLNTDKDTK IEAINILKQN EIALIKKFNI KKSCMKEKQK KLKEILYNTR KKLEENGYNP KQLEINLQKV YENYKYKPHF IIENHKYNDL GYIKRKLEKS IERKKENSQQ NYQNLKENIF NILIEQLKKE VNIEILKPII KEYLNNQKKI EYNKVFGIYH LELLEIIKNE KNSLTT
Validated NT Sequence
tctgtggtta aagaattttt ttcatttttt attatttcta ataattcaag atgatatata ccaaatactt tattgtattc tattttcttt tggttattca aatattcttt tataattggc tttagaattt caatatttac ttcttttttt agttgttcaa taaggatatt gaaaatattt tcctttaaat tttgataatt ttgttgagaa ttttcttttt ttctttcaat cgacttttct aatttacgtt ttatgtaacc taaatcatta tatttatgat tttcaataat aaaatggggc ttgtatttgt aattttcgta tactttttgt aaatttattt ctaattgttt gggattgtac ccgttttctt ctaatttttt cctagtgttg tatagaattt cttttaattt tttttgtttt tctttcatgc aagatttttt tatattgaat ttttttatta gggcaatttc attttgtttt aagatattta ttgcctcaat cttagtatct ttatcagtat ttaaatacaa aatagaaaga gcttcttccg ttttaaagtt acaattattg aaataatttc ttagttgata tttttctatt tctttaatct ttctttcttc ttttatatta tttttattac ttaaacactc cactgaattt acactactat ttttagaaac attgtcttta aaatggttat taactctaga tttaaatcta gagttttttc gttctttaaa gtacttgttg attttctggt aacattcttt tttaggatac tttagcttat agtaaatttc agttccacaa tttacaccca tgtgttggta gtaattcgtt gtgactttta atactttttc taatttataa agataatttt gcattgttct cagtgtagtg ggagccaaac catttctttt tagattttta ttatagtaat agagtatgtt ttgttgtgta tatttcttat cttttttgtt tagaaaatct actgttgatg taagatatat taacttgtgt tggtgtttgt tgtggcaagt gggatttttt gtggtgatta aaaaatcttt catatggtgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMK DFLITTKNPT CHNKHQHKLI YLTSTVDFLN KKDKKYTQQN ILYYYNKNLK RNGLAPTTLR TMQNYLYKLE KVLKVTTNYY QHMGVNCGTE IYYKLKYPKK ECYQKINKYF KERKNSRFKS RVNNHFKDNV SKNSSVNSVE CLSNKNNIKE ERKIKEIEKY QLRNYFNNCN FKTEEALSIL YLNTDKDTKI EAINILKQNE IALIKKFNIK KSCMKEKQKK LKEILYNTRK KLEENGYNPK QLEINLQKVY ENYKYKPHFI IENHKYNDLG YIKRKLEKSI ERKKENSQQN YQNLKENIFN ILIEQLKKEV NIEILKPIIK EYLNNQKKIE YNKVFGIYHL ELLEIIKNEK NSLTTEEFSI KAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag attttttaat caccacaaaa aatcccactt gccacaacaa acaccaacac aagttaatat atcttacatc aacagtagat tttctaaaca aaaaagataa gaaatataca caacaaaaca tactctatta ctataataaa aatctaaaaa gaaatggttt ggctcccact acactgagaa caatgcaaaa ttatctttat aaattagaaa aagtattaaa agtcacaacg aattactacc aacacatggg tgtaaattgt ggaactgaaa tttactataa gctaaagtat cctaaaaaag aatgttacca gaaaatcaac aagtacttta aagaacgaaa aaactctaga tttaaatcta gagttaataa ccattttaaa gacaatgttt ctaaaaatag tagtgtaaat tcagtggagt gtttaagtaa taaaaataat ataaaagaag aaagaaagat taaagaaata gaaaaatatc aactaagaaa ttatttcaat aattgtaact ttaaaacgga agaagctctt tctattttgt atttaaatac tgataaagat actaagattg aggcaataaa tatcttaaaa caaaatgaaa ttgccctaat aaaaaaattc aatataaaaa aatcttgcat gaaagaaaaa caaaaaaaat taaaagaaat tctatacaac actaggaaaa aattagaaga aaacgggtac aatcccaaac aattagaaat aaatttacaa aaagtatacg aaaattacaa atacaagccc cattttatta ttgaaaatca taaatataat gatttaggtt acataaaacg taaattagaa aagtcgattg aaagaaaaaa agaaaattct caacaaaatt atcaaaattt aaaggaaaat attttcaata tccttattga acaactaaaa aaagaagtaa atattgaaat tctaaagcca attataaaag aatatttgaa taaccaaaag aaaatagaat acaataaagt atttggtata tatcatcttg aattattaga aataataaaa aatgaaaaaa attctttaac cacagaagaa tttagcataa aggccgtatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat