BobuA.17768.b

Chemotaxis protein CheA (EC 2.7.13.3) (BB_0669)

CENTER ID: BobuA.17768.b
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17768.b.B1.GE38631 full length 1 864
BobuA.17768.b.B2.GE38632 nucleotide binding domain 521 864
BobuA.17768.b.B3.GE38633 histidine protein kinase 1 141
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.1060
RefSeq: NP_212803.1
RefSeq: WP_010889794.1
UniProt: Q44737

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEILDLENEE LLGVFFEEAQ NLVDILEENI MSLEDDPNNS DTIDEIFRAA HTLKGSSASL DMMELSDFTH IVEDVFDAIR DGKVNINNDL VDLLLSSLDV IKEMLALRID GKVYLNDISD LKSKLKQFLV IDDQTFIKRF DGNSIKNNFC LSESDLEEIR EGLGIGQKVL RISVVFNSNS NSEVENSGLK IFNILKNLGS VLHTIPKYEQ IIEDKFLKRV DYYLIYSDIE GVKKSLDSLN LIESYLVDEF NVKEELKKLA DEEIKDVDLD SNFVLNDNFD FTEDEISDLL LEVENQKLFK VRLDFVKDNP MATISGLQML QALKSLGKIF KSIPDSSELL ADKFFDFVIY YLISNTSEES IAKKINLPDV VSHFEIKNVN LESLKSVRLK EDDEAPFKEN KNIKKNSPIS VNLIRIDSKK IDYILNLVSE AVISKSSYNQ INSEMITLFY NFNYFYDYQE SFQRNFLIDL KIVFKDAGLT LEDEIESHIN SLMSFKMEKA LKDISELRNS FFRLLQNFKM TSGRLSRIIT DLHESVLKTR MLPISNIFSR FTRVVRDLSK KLNKIVNLKM EGEETELDKS VIDDLVDPLM HCVRNSMDHG LETVEERVKR GKSKAGTIIL RAKNEGNVIS IEIEDDGIGI DPKVIRRKLI EKGTIKEDAI YSDFELINLI FAPGFSTAVQ VTDLSGRGVG LDVVKKSIEK LNGTILVESE IGLGTIFKIK LPLTLVIIQG LLVKSGSETY VIPLNNVLET HRITEHDIKL LENYHEVYNL RDEVISVLRL DKLFNITRDD SLIEKFLIVV NTSNMKIAIV VDSILGEEDF VVKPIKDKFS SSAGIVGATT LGNGKVVLII DVFKLFDLQK DTKE
NT Sequence
atggaaatat tagatttgga aaatgaagag cttttaggag ttttttttga agaagctcaa aatcttgtag atatccttga agagaatatt atgtcattag aggatgatcc taataattct gatactattg atgaaatatt cagggcagct catactttaa aaggaagctc tgcttctctt gatatgatgg agttatctga ttttacccat attgtcgaag atgtttttga tgctattaga gatggtaagg taaatataaa taatgatctt gttgatctgc ttttaagttc attagatgtt atcaaggaaa tgcttgcgct tcgtattgat ggcaaggttt atttaaatga cataagtgat cttaaaagca aattaaagca atttttagta attgatgatc agacttttat taagagattt gatggaaatt caattaaaaa caatttttgt ctttcagaat cagatcttga ggagataaga gaagggctag gaattggaca aaaggtttta aggatcagtg ttgtttttaa ttcaaatagc aattctgaag ttgaaaatag tgggctaaaa atatttaata ttttaaaaaa tttaggatct gtacttcata caattcctaa atatgagcaa atcatagagg ataaattttt aaaaagggtt gattattatc tgatatattc agatatagaa ggcgtgaaaa aaagtttaga ttctttaaat ttaattgaaa gctatttggt tgatgaattt aacgtaaaag aagaattaaa aaagcttgca gatgaagaga ttaaagatgt tgatttagat tccaattttg ttttaaatga taattttgat tttacagagg atgagatttc tgatttatta cttgaggttg aaaatcaaaa gttatttaaa gttagattgg attttgtaaa agacaatcct atggctacta ttagtgggct tcaaatgctt caagcattaa aaagtcttgg taaaattttc aagtctatcc cagattctag cgaattattg gcagataagt tttttgattt tgtaatatat tacttaatat caaataccag tgaagaaagt attgctaaaa agattaattt accagatgtt gttagtcatt ttgaaatcaa aaatgttaat ttagaatctt taaagagcgt aaggctaaaa gaagatgatg aagcaccttt taaggaaaat aaaaatatta agaaaaatag tccaattagt gttaatttaa ttagaataga tagcaaaaaa atagattaca tattaaatct tgtcagtgag gctgtaataa gtaaatcatc ttataatcaa ataaattcag aaatgattac attattttat aattttaatt atttttatga ttatcaagaa agttttcaga gaaacttttt aattgattta aagatagttt tcaaagatgc aggcttaaca ttagaagatg agatcgaatc acatattaat tctttgatga gttttaaaat ggaaaaggct cttaaggata tatctgaatt gagaaattct tttttcagac ttcttcaaaa ttttaaaatg acctctgggc ggctgtcaag aataattaca gatttgcatg agagtgtttt aaaaaccaga atgttaccaa tatctaatat attttcaagg tttacaagag tcgtaagaga tctttcaaag aaattaaata agattgtgaa tcttaaaatg gaaggggaag aaactgagct tgataagtct gttatagatg accttgtaga tcctttgatg cattgtgtta gaaattcaat ggatcatggc cttgaaacag ttgaagagag agttaaaagg ggaaagagca aagcaggtac tataattttg cgtgccaaga atgaaggtaa tgtaatatca attgagattg aagatgatgg gattggtata gatccaaagg tcattaggcg caaattaatt gaaaagggaa caataaaaga agatgcaatt tactctgatt ttgaacttat taacttaatt tttgctcctg ggttttcaac agcagttcaa gtgacagacc tttcaggtag aggagttggt cttgacgttg tgaaaaaaag cattgaaaag cttaatggaa ctattttggt ggaatcagaa attggtcttg gaacaatttt taaaattaaa ctaccattga cgttagtgat tatacaaggt cttttagtaa agtcggggtc tgagacttat gttattcctt taaataatgt tcttgaaact catagaataa ctgaacatga tataaaattg cttgaaaatt atcatgaagt ttataattta agagatgaag tcatttctgt tctcaggctt gataaacttt ttaacataac aagggatgat tcattaatag aaaaattttt aatagttgtt aatactagca acatgaagat agcaattgtt gtagactcta ttcttggtga ggaagatttt gtagtaaagc ctattaagga taaattttca tcaagcgcgg gtatagttgg ggctactacg cttggcaatg gtaaggttgt attgataatt gatgttttta aactttttga tttacaaaag gatactaagg agtga
Details for BobuA.17768.b.B1.GE38631
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 99kDa
OBSERVED MW: 99kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME ILDLENEELL GVFFEEAQNL VDILEENIMS LEDDPNNSDT IDEIFRAAHT LKGSSASLDM MELSDFTHIV EDVFDAIRDG KVNINNDLVD LLLSSLDVIK EMLALRIDGK VYLNDISDLK SKLKQFLVID DQTFIKRFDG NSIKNNFCLS ESDLEEIREG LGIGQKVLRI SVVFNSNSNS EVENSGLKIF NILKNLGSVL HTIPKYEQII EDKFLKRVDY YLIYSDIEGV KKSLDSLNLI ESYLVDEFNV KEELKKLADE EIKDVDLDSN FVLNDNFDFT EDEISDLLLE VENQKLFKVR LDFVKDNPMA TISGLQMLQA LKSLGKIFKS IPDSSELLAD KFFDFVIYYL ISNTSEESIA KKINLPDVVS HFEIKNVNLE SLKSVRLKED DEAPFKENKN IKKNSPISVN LIRIDSKKID YILNLVSEAV ISKSSYNQIN SEMITLFYNF NYFYDYQESF QRNFLIDLKI VFKDAGLTLE DEIESHINSL MSFKMEKALK DISELRNSFF RLLQNFKMTS GRLSRIITDL HESVLKTRML PISNIFSRFT RVVRDLSKKL NKIVNLKMEG EETELDKSVI DDLVDPLMHC VRNSMDHGLE TVEERVKRGK SKAGTIILRA KNEGNVISIE IEDDGIGIDP KVIRRKLIEK GTIKEDAIYS DFELINLIFA PGFSTAVQVT DLSGRGVGLD VVKKSIEKLN GTILVESEIG LGTIFKIKLP LTLVIIQGLL VKSGSETYVI PLNNVLETHR ITEHDIKLLE NYHEVYNLRD EVISVLRLDK LFNITRDDSL IEKFLIVVNT SNMKIAIVVD SILGEEDFVV KPIKDKFSSS AGIVGATTLG NGKVVLIIDV FKLFDLQKDT KE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa tattagattt ggaaaatgaa gagcttttag gagttttttt tgaagaagct caaaatcttg tagatatcct tgaagagaat attatgtcat tagaggatga tcctaataat tctgatacta ttgatgaaat attcagggca gctcatactt taaaaggaag ctctgcttct cttgatatga tggagttatc tgattttacc catattgtcg aagatgtttt tgatgctatt agagatggta aggtaaatat aaataatgat cttgttgatc tgcttttaag ttcattagat gttatcaagg aaatgcttgc gcttcgtatt gatggcaagg tttatttaaa tgacataagt gatcttaaaa gcaaattaaa gcaattttta gtaattgatg atcagacttt tattaagaga tttgatggaa attcaattaa aaacaatttt tgtctttcag aatcagatct tgaggagata agagaagggc taggaattgg acaaaaggtt ttaaggatca gtgttgtttt taattcaaat agcaattctg aagttgaaaa tagtgggcta aaaatattta atattttaaa aaatttagga tctgtacttc atacaattcc taaatatgag caaatcatag aggataaatt tttaaaaagg gttgattatt atctgatata ttcagatata gaaggcgtga aaaaaagttt agattcttta aatttaattg aaagctattt ggttgatgaa tttaacgtaa aagaagaatt aaaaaagctt gcagatgaag agattaaaga tgttgattta gattccaatt ttgttttaaa tgataatttt gattttacag aggatgagat ttctgattta ttacttgagg ttgaaaatca aaagttattt aaagttagat tggattttgt aaaagacaat cctatggcta ctattagtgg gcttcaaatg cttcaagcat taaaaagtct tggtaaaatt ttcaagtcta tcccagattc tagcgaatta ttggcagata agttttttga ttttgtaata tattacttaa tatcaaatac cagtgaagaa agtattgcta aaaagattaa tttaccagat gttgttagtc attttgaaat caaaaatgtt aatttagaat ctttaaagag cgtaaggcta aaagaagatg atgaagcacc ttttaaggaa aataaaaata ttaagaaaaa tagtccaatt agtgttaatt taattagaat agatagcaaa aaaatagatt acatattaaa tcttgtcagt gaggctgtaa taagtaaatc atcttataat caaataaatt cagaaatgat tacattattt tataatttta attattttta tgattatcaa gaaagttttc agagaaactt tttaattgat ttaaagatag ttttcaaaga tgcaggctta acattagaag atgagatcga atcacatatt aattctttga tgagttttaa aatggaaaag gctcttaagg atatatctga attgagaaat tcttttttca gacttcttca aaattttaaa atgacctctg ggcggctgtc aagaataatt acagatttgc atgagagtgt tttaaaaacc agaatgttac caatatctaa tatattttca aggtttacaa gagtcgtaag agatctttca aagaaattaa ataagattgt gaatcttaaa atggaagggg aagaaactga gcttgataag tctgttatag atgaccttgt agatcctttg atgcattgtg ttagaaattc aatggatcat ggccttgaaa cagttgaaga gagagttaaa aggggaaaga gcaaagcagg tactataatt ttgcgtgcca agaatgaagg taatgtaata tcaattgaga ttgaagatga tgggattggt atagatccaa aggtcattag gcgcaaatta attgaaaagg gaacaataaa agaagatgca atttactctg attttgaact tattaactta atttttgctc ctgggttttc aacagcagtt caagtgacag acctttcagg tagaggagtt ggtcttgacg ttgtgaaaaa aagcattgaa aagcttaatg gaactatttt ggtggaatca gaaattggtc ttggaacaat ttttaaaatt aaactaccat tgacgttagt gattatacaa ggtcttttag taaagtcggg gtctgagact tatgttattc ctttaaataa tgttcttgaa actcatagaa taactgaaca tgatataaaa ttgcttgaaa attatcatga agtttataat ttaagagatg aagtcatttc tgttctcagg cttgataaac tttttaacat aacaagggat gattcattaa tagaaaaatt tttaatagtt gttaatacta gcaacatgaa gatagcaatt gttgtagact ctattcttgg tgaggaagat tttgtagtaa agcctattaa ggataaattt tcatcaagcg cgggtatagt tggggctact acgcttggca atggtaaggt tgtattgata attgatgttt ttaaactttt tgatttacaa aaggatacta aggagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BobuA.17768.b.B2.GE38632
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 39kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMT SGRLSRIITD LHESVLKTRM LPISNIFSRF TRVVRDLSKK LNKIVNLKME GEETELDKSV IDDLVDPLMH CVRNSMDHGL ETVEERVKRG KSKAGTIILR AKNEGNVISI EIEDDGIGID PKVIRRKLIE KGTIKEDAIY SDFELINLIF APGFSTAVQV TDLSGRGVGL DVVKKSIEKL NGTILVESEI GLGTIFKIKL PLTLVIIQGL LVKSGSETYV IPLNNVLETH RITEHDIKLL ENYHEVYNLR DEVISVLRLD KLFNITRDDS LIEKFLIVVN TSNMKIAIVV DSILGEEDFV VKPIKDKFSS SAGIVGATTL GNGKVVLIID VFKLF
Validated NT Sequence
tcaaaaagtt taaaaacatc aattatcaat acaaccttac cattgccaag cgtagtagcc ccaactatac ccgcgcttga tgaaaattta tccttaatag gctttactac aaaatcttcc tcaccaagaa tagagtctac aacaattgct atcttcatgt tgctagtatt aacaactatt aaaaattttt ctattaatga atcatccctt gttatgttaa aaagtttatc aagcctgaga acagaaatga cttcatctct taaattataa acttcatgat aattttcaag caattttata tcatgttcag ttattctatg agtttcaaga acattattta aaggaataac ataagtctca gaccccgact ttactaaaag accttgtata atcactaacg tcaatggtag tttaatttta aaaattgttc caagaccaat ttctgattcc accaaaatag ttccattaag cttttcaatg ctttttttca caacgtcaag accaactcct ctacctgaaa ggtctgtcac ttgaactgct gttgaaaacc caggagcaaa aattaagtta ataagttcaa aatcagagta aattgcatct tcttttattg ttcccttttc aattaatttg cgcctaatga cctttggatc tataccaatc ccatcatctt caatctcaat tgatattaca ttaccttcat tcttggcacg caaaattata gtacctgctt tgctctttcc ccttttaact ctctcttcaa ctgtttcaag gccatgatcc attgaatttc taacacaatg catcaaagga tctacaaggt catctataac agacttatca agctcagttt cttccccttc cattttaaga ttcacaatct tatttaattt ctttgaaaga tctcttacga ctcttgtaaa ccttgaaaat atattagata ttggtaacat tctggttttt aaaacactct catgcaaatc tgtaattatt cttgacagcc gcccagaggt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHTS GRLSRIITDL HESVLKTRML PISNIFSRFT RVVRDLSKKL NKIVNLKMEG EETELDKSVI DDLVDPLMHC VRNSMDHGLE TVEERVKRGK SKAGTIILRA KNEGNVISIE IEDDGIGIDP KVIRRKLIEK GTIKEDAIYS DFELINLIFA PGFSTAVQVT DLSGRGVGLD VVKKSIEKLN GTILVESEIG LGTIFKIKLP LTLVIIQGLL VKSGSETYVI PLNNVLETHR ITEHDIKLLE NYHEVYNLRD EVISVLRLDK LFNITRDDSL IEKFLIVVNT SNMKIAIVVD SILGEEDFVV KPIKDKFSSS AGIVGATTLG NGKVVLIIDV FKLFDLQKDT KE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacctctg ggcggctgtc aagaataatt acagatttgc atgagagtgt tttaaaaacc agaatgttac caatatctaa tatattttca aggtttacaa gagtcgtaag agatctttca aagaaattaa ataagattgt gaatcttaaa atggaagggg aagaaactga gcttgataag tctgttatag atgaccttgt agatcctttg atgcattgtg ttagaaattc aatggatcat ggccttgaaa cagttgaaga gagagttaaa aggggaaaga gcaaagcagg tactataatt ttgcgtgcca agaatgaagg taatgtaata tcaattgaga ttgaagatga tgggattggt atagatccaa aggtcattag gcgcaaatta attgaaaagg gaacaataaa agaagatgca atttactctg attttgaact tattaactta atttttgctc ctgggttttc aacagcagtt caagtgacag acctttcagg tagaggagtt ggtcttgacg ttgtgaaaaa aagcattgaa aagcttaatg gaactatttt ggtggaatca gaaattggtc ttggaacaat ttttaaaatt aaactaccat tgacgttagt gattatacaa ggtcttttag taaagtcggg gtctgagact tatgttattc ctttaaataa tgttcttgaa actcatagaa taactgaaca tgatataaaa ttgcttgaaa attatcatga agtttataat ttaagagatg aagtcatttc tgttctcagg cttgataaac tttttaacat aacaagggat gattcattaa tagaaaaatt tttaatagtt gttaatacta gcaacatgaa gatagcaatt gttgtagact ctattcttgg tgaggaagat tttgtagtaa agcctattaa ggataaattt tcatcaagcg cgggtatagt tggggctact acgcttggca atggtaaggt tgtattgata attgatgttt ttaaactttt tgatttacaa aaggatacta aggagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BobuA.17768.b.B3.GE38633
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 17kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME ILDLENEELL GVFFEEAQNL VDILEENIMS LEDDPNNSDT IDEIFRAAHT LKGSSASLDM MELSDFTHIV EDVFDAIRDG KVNINNDLVD LLLSSLDVIK EMLALRIDGK VYLNDISDLK SKLKQFLVID DQTFIKRFD
Validated NT Sequence
atcttactca cttaatcaaa tctcttaata aaagtctgat catcaattac taaaaattgc tttaatttgc ttttaagatc acttatgtca tttaaataaa ccttgccatc aatacgaagc gcaagcattt ccttgataac atctaatgaa cttaaaagca gatcaacaag atcattattt atatttacct taccatctct aatagcatca aaaacatctt cgacaatatg ggtaaaatca gataactcca tcatatcaag agaagcagag cttcctttta aagtatgagc tgccctgaat atttcatcaa tagtatcaga attattagga tcatcctcta atgacataat attctcttca aggatatcta caagattttg agcttcttca aaaaaaactc ctaaaagctc ttcattttcc aaatctaata tttccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHME ILDLENEELL GVFFEEAQNL VDILEENIMS LEDDPNNSDT IDEIFRAAHT LKGSSASLDM MELSDFTHIV EDVFDAIRDG KVNINNDLVD LLLSSLDVIK EMLALRIDGK VYLNDISDLK SKLKQFLVID DQTFIKRFD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa tattagattt ggaaaatgaa gagcttttag gagttttttt tgaagaagct caaaatcttg tagatatcct tgaagagaat attatgtcat tagaggatga tcctaataat tctgatacta ttgatgaaat attcagggca gctcatactt taaaaggaag ctctgcttct cttgatatga tggagttatc tgattttacc catattgtcg aagatgtttt tgatgctatt agagatggta aggtaaatat aaataatgat cttgttgatc tgcttttaag ttcattagat gttatcaagg aaatgcttgc gcttcgtatt gatggcaagg tttatttaaa tgacataagt gatcttaaaa gcaaattaaa gcaattttta gtaattgatg atcagacttt tattaagaga tttgattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t