BobuA.17768.a

Chemotaxis histidine kinase (CheA-1) (BB_0567)

CENTER ID: BobuA.17768.a
ORGANISM: Borrelia burgdorferi B31
ASSOCIATED DISEASE: Lyme disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BobuA.17768.a.A1.GE32515 Full length( BobuA.17768.a ) 1 714
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224326.49.peg.958
RefSeq: NP_212701.1
UniProt: O51515

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSSDVIDKF KNSFKEESIE NISDIEQALL NLEVSSDQDI VNSIFRNLHT IKGSSGMFGF NFTASLVHEI ETVLDVVKNG KAAFNQAAID ATLMSVDFIR ELIEGDEVIS EIDFDKRKQF LVNEIRKVLD ISNDVKGAFQ EALENDFSKS DNSSVLEESV KVNSENKFDD EALQSEFKSY KILFSPAKGI LFHGHKPINL LSKLINLGSG YVRAKVDNIP DLELISPDNV YVDWEIRLDT EESRESIEDI FTFLDSQSKI DIQELDKCLE ANEGDNVESK NFNLLHLDRN SKDLTNSSFE KSAFVGKSFF NGDKNRSNIQ DDTARSKVNI ASIKVDSKKL DHLVNLVGEL VTIQSKLSKE AENRNSNILN SISAEFSLLI NELRDYTTGL RTVPIEILFV KFQRIVKDLS TSLGKSILYH ASGGDTVLDK SIIEKLNEPL VHLIRNSIDH GIESAQERES LGKDPKGIIK LSACQSGDSV IVIIEDDGRG LDKNKILKKA IERNIISDSV AKTLSEIDVY NLIFEPGFST ASSVTDISGR GVGMDVVKKQ VESLRGHVVL ESEFGKYTRI KLIFPLTLAI IEGWLVRVKD EHFIVPLSNV ESCLESNKLI SQIDGIETKS NVMNYRGSMI SYIRLREFFQ VSSEKSLSEQ VVVVNTNSGK MGIVVDEVLG QHQTVIKALG KIYSRVEGVS GATILGDGSL ALVVDIDAIT KLIK
NT Sequence
ATGGATAGTA GTGATGTTAT TGATAAATTT AAGAATTCCT TTAAGGAAGA ATCAATAGAA AATATTTCAG ATATTGAGCA AGCGCTTCTT AATCTTGAGG TAAGCTCAGA TCAAGATATT GTTAATTCTA TTTTTAGAAA TTTACATACC ATAAAGGGAA GTTCTGGTAT GTTTGGCTTT AATTTTACAG CATCGCTTGT CCACGAAATA GAAACAGTTC TTGATGTTGT AAAAAATGGT AAGGCTGCTT TTAATCAGGC GGCTATTGAT GCTACCTTAA TGTCTGTTGA TTTTATTAGA GAGCTTATTG AAGGCGATGA AGTAATTTCT GAGATCGACT TTGATAAGCG TAAACAGTTT TTGGTAAATG AAATTAGAAA GGTTCTTGAT ATTTCTAATG ATGTTAAGGG AGCTTTTCAA GAAGCTTTAG AAAATGATTT TTCAAAGTCT GATAACAGTT CGGTTTTGGA AGAGTCTGTT AAAGTAAATT CAGAGAATAA ATTTGATGAT GAGGCTTTGC AGTCTGAATT TAAGAGTTAC AAAATTCTTT TTTCTCCGGC TAAGGGTATT TTGTTTCATG GGCACAAGCC TATAAATTTA TTGAGCAAGT TGATTAATTT GGGTAGTGGT TATGTTAGGG CCAAAGTAGA TAACATTCCT GATTTAGAGC TTATCTCTCC TGATAATGTT TATGTTGATT GGGAGATAAG GTTAGATACA GAAGAGAGTA GAGAGAGCAT TGAAGATATT TTTACATTTT TAGATTCTCA ATCAAAAATT GATATTCAAG AATTGGATAA ATGTTTAGAG GCAAATGAAG GTGATAATGT AGAGTCTAAA AATTTTAATT TGTTGCACTT GGACAGAAAC AGCAAAGACT TGACTAATTC TTCATTTGAA AAATCGGCAT TTGTTGGGAA ATCCTTTTTC AATGGAGATA AAAATAGATC TAATATTCAA GATGATACTG CTAGAAGCAA GGTTAATATT GCTAGTATTA AGGTGGATTC TAAAAAGCTT GATCATTTGG TAAATCTTGT TGGAGAACTT GTTACAATAC AATCAAAACT TTCAAAAGAA GCTGAAAATA GGAATAGTAA TATTTTAAAT TCAATATCAG CAGAATTTTC TTTGCTTATT AATGAGCTTA GGGATTATAC AACAGGGCTT AGAACAGTTC CTATTGAGAT TTTGTTTGTA AAATTTCAAA GGATAGTAAA AGATCTATCT ACTAGCCTTG GTAAGTCAAT TCTTTATCAT GCTTCTGGAG GCGACACTGT TCTTGACAAA AGTATTATTG AAAAGCTAAA TGAACCTTTA GTTCATTTAA TTCGCAACTC AATAGATCAT GGAATTGAAT CGGCTCAAGA GAGAGAAAGT TTAGGTAAAG ATCCCAAAGG TATTATTAAG CTTTCAGCGT GTCAATCTGG GGATTCTGTT ATTGTTATTA TTGAGGATGA CGGAAGAGGG CTTGATAAGA ATAAAATACT TAAAAAAGCT ATAGAGCGCA ATATAATTTC TGATTCAGTT GCCAAAACCT TATCAGAGAT TGATGTTTAT AATTTGATTT TTGAGCCTGG ATTTTCAACT GCAAGTTCTG TTACTGATAT ATCAGGTCGT GGAGTTGGCA TGGATGTTGT CAAAAAACAG GTTGAATCTT TAAGGGGGCA TGTTGTACTT GAAAGTGAAT TTGGTAAATA TACTAGAATC AAGTTAATTT TTCCATTGAC TTTGGCTATT ATTGAGGGTT GGCTTGTTAG AGTAAAAGAT GAGCATTTTA TTGTTCCTCT TTCTAATGTT GAATCTTGTT TAGAATCTAA TAAGTTAATT TCTCAAATAG ATGGAATTGA GACTAAAAGC AATGTAATGA ATTATAGGGG CAGCATGATT AGTTATATTA GGCTTAGAGA GTTTTTTCAG GTTTCTAGCG AGAAGAGTTT AAGTGAGCAA GTTGTTGTTG TAAATACAAA TAGTGGGAAA ATGGGCATTG TGGTTGACGA AGTTTTGGGT CAACATCAAA CTGTCATAAA GGCTTTGGGT AAAATTTATT CTCGAGTAGA AGGGGTTTCT GGAGCTACTA TACTTGGTGA TGGAAGTTTG GCTTTGGTTG TTGACATAGA TGCAATAACT AAACTTATAA AA
Details for BobuA.17768.a.A1.GE32515
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 81kDa
OBSERVED MW: 83kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 0
PERCENT COVERAGE: 14
Validated AA Sequence
MXLXXXXGXI XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXTXXXX XXXXXXXXXX XXXX
Validated NT Sequence
cnnnnnattt tnnttncttt aagaagnnag nnataccang gnnctcatca ccatcaccat catannnnta cnnnnnnngc tcagacccnn ggtnnnnnnt cgatggatag tanngangtt attgataaac agcacgaaca agcatanang caataactaa acttataaaa taataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgannn ggctgctgcc accgctgacn nnnactnnnn nnannnnnnn nnnnnnnnng tgcgtggccn nnnnnnnnna ncnnaatgng ncttnnnnnn nnannngggn ggatannnnt nnnnnnttnt ngnnnnanaa nnnggncncn nnnnnnnnnn annannnnnt nnnnnanntn nnannnnnan annnnnnnnn nnnnnnnnnn nnngnnnngn nnnnnnncan ncngnnannn nnnannnnnn ngncnnnann ntnnnnnaan nngnnnnnnn ntnnnnnnan tnanncnnnn nnantanann nnnnnnntnn nnnnnnnnnt nnnnnnnnng acnanannnn nnnnnnnnnn ntnnnncnac nanngcanan tnnnnnnnnt antnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDSSDVIDK FKNSFKEESI ENISDIEQAL LNLEVSSDQD IVNSIFRNLH TIKGSSGMFG FNFTASLVHE IETVLDVVKN GKAAFNQAAI DATLMSVDFI RELIEGDEVI SEIDFDKRKQ FLVNEIRKVL DISNDVKGAF QEALENDFSK SDNSSVLEES VKVNSENKFD DEALQSEFKS YKILFSPAKG ILFHGHKPIN LLSKLINLGS GYVRAKVDNI PDLELISPDN VYVDWEIRLD TEESRESIED IFTFLDSQSK IDIQELDKCL EANEGDNVES KNFNLLHLDR NSKDLTNSSF EKSAFVGKSF FNGDKNRSNI QDDTARSKVN IASIKVDSKK LDHLVNLVGE LVTIQSKLSK EAENRNSNIL NSISAEFSLL INELRDYTTG LRTVPIEILF VKFQRIVKDL STSLGKSILY HASGGDTVLD KSIIEKLNEP LVHLIRNSID HGIESAQERE SLGKDPKGII KLSACQSGDS VIVIIEDDGR GLDKNKILKK AIERNIISDS VAKTLSEIDV YNLIFEPGFS TASSVTDISG RGVGMDVVKK QVESLRGHVV LESEFGKYTR IKLIFPLTLA IIEGWLVRVK DEHFIVPLSN VESCLESNKL ISQIDGIETK SNVMNYRGSM ISYIRLREFF QVSSEKSLSE QVVVVNTNSG KMGIVVDEVL GQHQTVIKAL GKIYSRVEGV SGATILGDGS LALVVDIDAI TKLIK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatagtagt gatgttattg ataaatttaa gaattccttt aaggaagaat caatagaaaa tatttcagat attgagcaag cgcttcttaa tcttgaggta agctcagatc aagatattgt taattctatt tttagaaatt tacataccat aaagggaagt tctggtatgt ttggctttaa ttttacagca tcgcttgtcc acgaaataga aacagttctt gatgttgtaa aaaatggtaa ggctgctttt aatcaggcgg ctattgatgc taccttaatg tctgttgatt ttattagaga gcttattgaa ggcgatgaag taatttctga gatcgacttt gataagcgta aacagttttt ggtaaatgaa attagaaagg ttcttgatat ttctaatgat gttaagggag cttttcaaga agctttagaa aatgattttt caaagtctga taacagttcg gttttggaag agtctgttaa agtaaattca gagaataaat ttgatgatga ggctttgcag tctgaattta agagttacaa aattcttttt tctccggcta agggtatttt gtttcatggg cacaagccta taaatttatt gagcaagttg attaatttgg gtagtggtta tgttagggcc aaagtagata acattcctga tttagagctt atctctcctg ataatgttta tgttgattgg gagataaggt tagatacaga agagagtaga gagagcattg aagatatttt tacattttta gattctcaat caaaaattga tattcaagaa ttggataaat gtttagaggc aaatgaaggt gataatgtag agtctaaaaa ttttaatttg ttgcacttgg acagaaacag caaagacttg actaattctt catttgaaaa atcggcattt gttgggaaat cctttttcaa tggagataaa aatagatcta atattcaaga tgatactgct agaagcaagg ttaatattgc tagtattaag gtggattcta aaaagcttga tcatttggta aatcttgttg gagaacttgt tacaatacaa tcaaaacttt caaaagaagc tgaaaatagg aatagtaata ttttaaattc aatatcagca gaattttctt tgcttattaa tgagcttagg gattatacaa cagggcttag aacagttcct attgagattt tgtttgtaaa atttcaaagg atagtaaaag atctatctac tagccttggt aagtcaattc tttatcatgc ttctggaggc gacactgttc ttgacaaaag tattattgaa aagctaaatg aacctttagt tcatttaatt cgcaactcaa tagatcatgg aattgaatcg gctcaagaga gagaaagttt aggtaaagat cccaaaggta ttattaagct ttcagcgtgt caatctgggg attctgttat tgttattatt gaggatgacg gaagagggct tgataagaat aaaatactta aaaaagctat agagcgcaat ataatttctg attcagttgc caaaacctta tcagagattg atgtttataa tttgattttt gagcctggat tttcaactgc aagttctgtt actgatatat caggtcgtgg agttggcatg gatgttgtca aaaaacaggt tgaatcttta agggggcatg ttgtacttga aagtgaattt ggtaaatata ctagaatcaa gttaattttt ccattgactt tggctattat tgagggttgg cttgttagag taaaagatga gcattttatt gttcctcttt ctaatgttga atcttgttta gaatctaata agttaatttc tcaaatagat ggaattgaga ctaaaagcaa tgtaatgaat tataggggca gcatgattag ttatattagg cttagagagt tttttcaggt ttctagcgag aagagtttaa gtgagcaagt tgttgttgta aatacaaata gtgggaaaat gggcattgtg gttgacgaag ttttgggtca acatcaaact gtcataaagg ctttgggtaa aatttattct cgagtagaag gggtttctgg agctactata cttggtgatg gaagtttggc tttggttgtt gacatagatg caataactaa acttataaaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa