BobuA.17750.a

4-alpha-glucanotransferase (MalQ)

CENTER ID:	BobuA.17750.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.17750.a.A1.GE32514	Full length( BobuA.17750.a )		1	506

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_133321
PATRIC ID:	fig\|224326.49.peg.563
RefSeq:	NP_212300.1
UniProt:	O51188

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKYKKTRINL NLKRKSGILL NISSLPSKYG IGDLGKGAYK FIDFLFASSQ SYWQMFAYSP IDFTRSPPYS IFSAFAGNVY YIDLEALDKF IDSDLSLLKE NETRYSDLKK ISFKDKFLKE AALNFINRAS ADEVRSFEKF KKKSSYWLLD FASFVAFKEY FFKESKNAFN VLFDRGILKR NEKDLFKLRN ILSKEIKVQE VLQYFFFSQF QALKRYANDK GIELIMNVPF FIAYDSADVW AYQKYFKLRF DASKDKIAGI SPDYFLEQEQ AWDSAAYSWN VLKKFKYEWW AKRIGVLRKY ADIIKIDHFR GFVSTWEVSA GESYAFNGLW VKSPGRDFFN FILNEIKDLK IWVEDFQNDL EDVSRLRDFF NFPGMKIMNL AFDFDSSNQN LPHNYIKNCI VYTGSGDNDT IREFINSLDD LHKKYIFDYL NTNENFVVWD MIRSAMGSVS DNVIVSMQDY INLGDKFSAN IPKSTLDNCI FRLLESDLDA TLSQNISFIT RLYGRA

NT Sequence

ATGAAATATA AAAAAACAAG GATTAATTTA AACTTAAAAA GAAAAAGTGG TATTTTGCTT AACATAAGCT CTTTACCATC TAAATATGGC ATTGGAGATT TAGGGAAAGG AGCTTATAAA TTTATAGATT TTTTGTTTGC ATCTTCTCAA AGTTATTGGC AAATGTTTGC TTATTCCCCT ATTGATTTTA CAAGATCTCC CCCTTATTCA ATTTTTTCGG CTTTTGCTGG CAATGTTTAT TATATTGATT TAGAAGCCCT TGACAAATTT ATAGATTCAG ATTTAAGTCT TTTAAAAGAA AATGAAACCA GGTATTCTGA TTTAAAAAAA ATAAGTTTTA AAGATAAATT TCTTAAAGAA GCCGCTTTAA ATTTTATTAA TAGAGCTTCG GCTGATGAGG TTAGAAGCTT TGAAAAATTT AAAAAAAAGT CTTCTTATTG GCTTTTAGAT TTTGCAAGTT TTGTTGCGTT TAAAGAATAT TTTTTTAAAG AGTCAAAGAA TGCTTTTAAT GTTCTTTTTG ATAGAGGAAT TCTTAAAAGA AATGAAAAAG ATTTATTTAA ATTGAGAAAT ATCCTTTCAA AAGAGATTAA AGTGCAAGAG GTATTGCAGT ATTTCTTTTT TTCACAGTTT CAAGCTTTAA AGCGCTATGC AAATGATAAG GGGATTGAGC TAATTATGAA CGTGCCTTTT TTTATAGCAT ATGACTCTGC TGATGTTTGG GCATACCAAA AATATTTTAA GCTAAGATTT GATGCAAGCA AAGATAAGAT TGCAGGAATT TCTCCAGATT ATTTTTTAGA ACAAGAGCAA GCTTGGGATA GTGCAGCTTA TAGCTGGAAT GTTTTAAAGA AATTTAAGTA TGAATGGTGG GCAAAAAGGA TTGGGGTTTT AAGAAAGTAT GCAGATATAA TTAAAATTGA TCATTTCAGA GGTTTTGTTT CTACTTGGGA AGTTAGTGCG GGCGAGTCTT ATGCTTTTAA TGGGTTGTGG GTAAAGTCGC CAGGACGAGA TTTTTTCAAT TTTATTTTAA ATGAAATTAA AGATTTAAAA ATTTGGGTTG AAGATTTTCA AAATGATCTT GAAGATGTTT CAAGACTAAG AGATTTTTTT AATTTTCCAG GAATGAAAAT AATGAATCTT GCCTTTGATT TTGATTCAAG CAATCAAAAT CTTCCTCATA ATTATATTAA AAATTGCATA GTTTACACAG GAAGTGGTGA TAATGATACA ATACGAGAGT TTATTAATTC TTTAGATGAT TTGCATAAAA AGTACATTTT TGATTATTTA AATACAAATG AAAATTTTGT TGTTTGGGAC ATGATAAGAA GTGCTATGGG TAGTGTTTCT GATAATGTGA TAGTGTCAAT GCAAGATTAT ATTAATTTAG GAGATAAATT TAGTGCAAAT ATCCCAAAAA GCACTTTAGA TAATTGTATT TTTAGATTAT TAGAAAGTGA TTTAGATGCT ACTTTGAGTC AAAATATAAG TTTTATTACA AGGCTTTATG GCAGGGCT

Details for BobuA.17750.a.A1.GE32514

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	61kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	46

Validated AA Sequence

Validated NT Sequence

gntnancttt nnnaaggaga nataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgaaatataa aaaaacaagg attaatttaa acttaaaaag aaaaagtggt attttgctta acataagctc tttaccatct aaatatggca ttggagattt agggaaagga gcttataaat ttatagattt tttgtttgca tcttctcaaa gttattggca aatgtttgct tattccccta ttgattttac aagatctccc ccttattcaa ttttttcggc ttttgctggc aatgtttatt atattgattt agaagccctt gacaaattta tagattcaga tttaagtctt ttaaaagaaa atgaaaccag gtattctgat ttaaaaaaaa taagttttaa agataaattt cttaaagaag ccgctttaaa ttttattaat agagcttcgg ctgatgaggt tagaagcttt gaaaaattta aaaaaaagtc ttcttattgg cttttagatt ttgcaagttt tgttgcgttt aaagaatatt tttttaaaga gtcaaagaat gcttttaatg ttctttttga tagaggaatt cttaaaagaa atgaaaaaga tttatttaaa ttgagaaata tcctttcaaa agagattaaa gtgcaagagg tattgcagta tttctttttt tcacagtttc aagctttaaa gcgctatgca aatgataann nnntgagcta attatgaacg tgcctttttt tatagcatat gactctgctg atgtttgggc ataccnaaaa tattttaagc taaganttga tgcaagcaaa gataagattg caggaatttc tccagantat tttttanaac aagagcaagc ttgggatagn gcagcttata gctggnatgn tttaaagaaa tttnagtatg aatggtgggc aaaanggatn ggggtttnan aaagtatgcn natannnnaa aantgatcat ttcnnnnntt ngntnntacn nnnnnnnann nnnggcnant ctnngntttn nngggtnnnn ggnnanntcn cngnnnannn ttttnnnttn nntttnannn annnnnantn naaanngnnn ngnnnannnn annnannntn nnnnnnnntn tttnnntnnn nnnnnnannn nnnnnnnnnn cc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKYKKTRIN LNLKRKSGIL LNISSLPSKY GIGDLGKGAY KFIDFLFASS QSYWQMFAYS PIDFTRSPPY SIFSAFAGNV YYIDLEALDK FIDSDLSLLK ENETRYSDLK KISFKDKFLK EAALNFINRA SADEVRSFEK FKKKSSYWLL DFASFVAFKE YFFKESKNAF NVLFDRGILK RNEKDLFKLR NILSKEIKVQ EVLQYFFFSQ FQALKRYAND KGIELIMNVP FFIAYDSADV WAYQKYFKLR FDASKDKIAG ISPDYFLEQE QAWDSAAYSW NVLKKFKYEW WAKRIGVLRK YADIIKIDHF RGFVSTWEVS AGESYAFNGL WVKSPGRDFF NFILNEIKDL KIWVEDFQND LEDVSRLRDF FNFPGMKIMN LAFDFDSSNQ NLPHNYIKNC IVYTGSGDND TIREFINSLD DLHKKYIFDY LNTNENFVVW DMIRSAMGSV SDNVIVSMQD YINLGDKFSA NIPKSTLDNC IFRLLESDLD ATLSQNISFI TRLYGRA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatataaa aaaacaagga ttaatttaaa cttaaaaaga aaaagtggta ttttgcttaa cataagctct ttaccatcta aatatggcat tggagattta gggaaaggag cttataaatt tatagatttt ttgtttgcat cttctcaaag ttattggcaa atgtttgctt attcccctat tgattttaca agatctcccc cttattcaat tttttcggct tttgctggca atgtttatta tattgattta gaagcccttg acaaatttat agattcagat ttaagtcttt taaaagaaaa tgaaaccagg tattctgatt taaaaaaaat aagttttaaa gataaatttc ttaaagaagc cgctttaaat tttattaata gagcttcggc tgatgaggtt agaagctttg aaaaatttaa aaaaaagtct tcttattggc ttttagattt tgcaagtttt gttgcgttta aagaatattt ttttaaagag tcaaagaatg cttttaatgt tctttttgat agaggaattc ttaaaagaaa tgaaaaagat ttatttaaat tgagaaatat cctttcaaaa gagattaaag tgcaagaggt attgcagtat ttcttttttt cacagtttca agctttaaag cgctatgcaa atgataaggg gattgagcta attatgaacg tgcctttttt tatagcatat gactctgctg atgtttgggc ataccaaaaa tattttaagc taagatttga tgcaagcaaa gataagattg caggaatttc tccagattat tttttagaac aagagcaagc ttgggatagt gcagcttata gctggaatgt tttaaagaaa tttaagtatg aatggtgggc aaaaaggatt ggggttttaa gaaagtatgc agatataatt aaaattgatc atttcagagg ttttgtttct acttgggaag ttagtgcggg cgagtcttat gcttttaatg ggttgtgggt aaagtcgcca ggacgagatt ttttcaattt tattttaaat gaaattaaag atttaaaaat ttgggttgaa gattttcaaa atgatcttga agatgtttca agactaagag atttttttaa ttttccagga atgaaaataa tgaatcttgc ctttgatttt gattcaagca atcaaaatct tcctcataat tatattaaaa attgcatagt ttacacagga agtggtgata atgatacaat acgagagttt attaattctt tagatgattt gcataaaaag tacatttttg attatttaaa tacaaatgaa aattttgttg tttgggacat gataagaagt gctatgggta gtgtttctga taatgtgata gtgtcaatgc aagattatat taatttagga gataaattta gtgcaaatat cccaaaaagc actttagata attgtatttt tagattatta gaaagtgatt tagatgctac tttgagtcaa aatataagtt ttattacaag gctttatggc agggcttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa