SSGCID: Target Page

BobuA.17690.a

basic membrane protein A (bmpA) (BB_0383)

CENTER ID:	BobuA.17690.a
ORGANISM:	Borrelia burgdorferi B31
ASSOCIATED DISEASE:	Lyme disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.17690.a.B2.GE39136	mature protein		20	339

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BobuA.17690.a.B2.PW37758	mature protein		20	339

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224326.49.peg.778
RefSeq:	NP_212517.1
RefSeq:	WP_002656850.1
UniProt:	Q45010

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKILLLILL ESIVFLSCSG KGSLGSEIPK VSLIIDGTFD DKSFNESALN GVKKVKEEFK IELVLKESSS NSYLSDLEGL KDAGSDLIWL IGYRFSDVAK VAALQNPDMK YAIIDPIYSN DPIPANLVGM TFRAQEGAFL TGYIAAKLSK TGKIGFLGGI EGEIVDAFRY GYEAGAKYAN KDIKISTQYI GSFADLEAGR SVATRMYSDE IDIIHHAAGL GGIGAIEVAK ELGSGHYIIG VDEDQAYLAP DNVITSTTKD VGRALNIFTS NHLKTNTFEG GKLINYGLKE GVVGFVRNPK MISFELEKEI DNLSSKIINK EIIVPSNKES YEKFLKEFI

NT Sequence

atgaataaaa tattgttgtt gattttgctt gagagtattg tttttttatc ttgtagtggt aaaggtagtc ttgggagcga aattcctaag gtatctttaa taattgatgg aacttttgat gataaatctt ttaatgagag tgctttaaat ggcgtaaaaa aagttaaaga agaatttaaa attgagcttg ttttaaaaga atcctcatca aattcttatt tatctgatct tgaagggctt aaggatgcgg gctcagattt aatttggctt attgggtata gatttagcga tgtggccaag gttgcggctc ttcaaaatcc cgatatgaaa tatgcaatta ttgatcctat ttattctaac gatcctattc ctgcaaattt ggtgggcatg acctttagag ctcaagaggg tgcattttta acgggttata ttgctgcaaa actttctaaa acaggtaaaa ttggattttt agggggaata gaaggcgaga tagtagatgc ttttaggtat gggtatgaag ctggtgctaa gtatgctaat aaagatataa agatatctac tcagtatatt ggtagttttg ctgaccttga agctggtaga agcgttgcaa ctagaatgta ttctgatgag atagacatta ttcatcatgc tgcaggcctt ggaggaattg gggctattga ggttgcaaaa gaacttggtt ctgggcatta cattattgga gttgatgaag atcaagcata tcttgctcct gacaatgtaa taacatctac aactaaagat gttggtagag ctttaaatat ttttacatct aaccatttaa aaactaatac tttcgaaggt ggcaaattaa taaattatgg ccttaaagaa ggagttgtgg ggtttgtaag aaatcctaaa atgatttcct ttgaacttga aaaagaaatt gacaatcttt ctagcaaaat aatcaacaaa gaaattattg ttccatctaa taaagaaagt tatgagaagt ttcttaaaga atttatttaa

Details for BobuA.17690.a.B2.GE39136

HARVESTED ON:	5/6/2015
SEQUENCED ON:	5/6/2015
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHXHHHMG KGSLGSEIPK VSLIIDGTFD DKSFNESALN GVKKVKEEFK IELVLKESSS NSYLSDLEGL KDAGSDLIWL IGYRFSDVAK VAALQNPDMK YAIIDPIYSN DPIPANLVGM TFRAQEGAFL TGYIAAKLSK TGKIGFLGGI EGEIVDAFRY GYEAGAKYAN KDIKISTQYI GSFADLEAGR SVATRMYSDE IDIIHHAASL GGIGAIEVAK ELGSGHYIIG VDEDQAYLAP DNVITSTTKD VGRALNIFTS NHLKTNTFEG GKLINYGLKE GVVGFVRNPK MISFELEKEI DNLSSKIINK EIIVPSNKES XXXXLKEFI

Validated NT Sequence

ttactcactt aaataaattc tttaagaann nnnnnnnnac tttctttatt agatggaaca ataatttctt tgttgattat tttgctagaa agattgtcaa tttctttttc aagttcaaag gaaatcattt taggatttct tacaaacccc acaactcctt ctttaaggcc ataatttatt aatttgccac cttcgaaagt attagttttt aaatggttag atgtaaaaat atttaaagct ctaccaacat ctttagttgt agatgttatt acattgtcag gagcaagata tgcttgatct tcatcaactc caataatgta atgcccagaa ccaagttctt ttgcaacctc aatagcccca attcctccaa ggcttgcagc atgatgaata atgtctatct catcagaata catcctagtt gcaacgcttc taccagcttc aaggtcagca aaactaccaa tatactgagt agatatcttt atatctttat tagcatactt agcaccagct tcatacccat acctaaaagc atctactatc tcgccttcta ttccccctaa aaatccaatt ttacctgttt tagaaagttt tgcagcaata taacccgtta aaaatgcacc ctcttgagct ctaaaggtca tgccgaccaa atttgcagga ataggatcgt tagaataaat aggatcaata attgcatatt tcatatcggg attttgaaga gccgcaacct tggccacatc gctaaatcta tacccaataa gccaaattaa atctgagccc gcatccttaa gcccttcaag atcagataaa taagaatttg atgaggattc ttttaaaaca agctcaattt taaattcttc tttaactttt tttacgccat ttaaagcact ctcattaaaa gatttatcat caaaagttcc atcaattatt aaagatacct taggaatttc gctcccaaga ctacctttac ccatatggtg gtggtngtgg tgagccat

Expected Protein Sequence

MAHHHHHHGK GSLGSEIPKV SLIIDGTFDD KSFNESALNG VKKVKEEFKI ELVLKESSSN SYLSDLEGLK DAGSDLIWLI GYRFSDVAKV AALQNPDMKY AIIDPIYSND PIPANLVGMT FRAQEGAFLT GYIAAKLSKT GKIGFLGGIE GEIVDAFRYG YEAGAKYANK DIKISTQYIG SFADLEAGRS VATRMYSDEI DIIHHAAGLG GIGAIEVAKE LGSGHYIIGV DEDQAYLAPD NVITSTTKDV GRALNIFTSN HLKTNTFEGG KLINYGLKEG VVGFVRNPKM ISFELEKEID NLSSKIINKE IIVPSNKESY EKFLKEFI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtaaag gtagtcttgg gagcgaaatt cctaaggtat ctttaataat tgatggaact tttgatgata aatcttttaa tgagagtgct ttaaatggcg taaaaaaagt taaagaagaa tttaaaattg agcttgtttt aaaagaatcc tcatcaaatt cttatttatc tgatcttgaa gggcttaagg atgcgggctc agatttaatt tggcttattg ggtatagatt tagcgatgtg gccaaggttg cggctcttca aaatcccgat atgaaatatg caattattga tcctatttat tctaacgatc ctattcctgc aaatttggtg ggcatgacct ttagagctca agagggtgca tttttaacgg gttatattgc tgcaaaactt tctaaaacag gtaaaattgg atttttaggg ggaatagaag gcgagatagt agatgctttt aggtatgggt atgaagctgg tgctaagtat gctaataaag atataaagat atctactcag tatattggta gttttgctga ccttgaagct ggtagaagcg ttgcaactag aatgtattct gatgagatag acattattca tcatgctgca ggccttggag gaattggggc tattgaggtt gcaaaagaac ttggttctgg gcattacatt attggagttg atgaagatca agcatatctt gctcctgaca atgtaataac atctacaact aaagatgttg gtagagcttt aaatattttt acatctaacc atttaaaaac taatactttc gaaggtggca aattaataaa ttatggcctt aaagaaggag ttgtggggtt tgtaagaaat cctaaaatga tttcctttga acttgaaaaa gaaattgaca atctttctag caaaataatc aacaaagaaa ttattgttcc atctaataaa gaaagttatg agaagtttct taaagaattt atttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BobuA.17690.a.B2.PW37758

PURIFICATION DATe:	8/3/2015
CONCENTRATION:	28mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	19
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGTAAAG GTAGTCTTGG GAGCGAAATT CCTAAGGTAT CTTTAATAAT TGATGGAACT TTTGATGATA AATCTTTTAA TGAGAGTGCT TTAAATGGCG TAAAAAAAGT TAAAGAAGAA TTTAAAATTG AGCTTGTTTT AAAAGAATCC TCATCAAATT CTTATTTATC TGATCTTGAA GGGCTTAAGG ATGCGGGCTC AGATTTAATT TGGCTTATTG GGTATAGATT TAGCGATGTG GCCAAGGTTG CGGCTCTTCA AAATCCCGAT ATGAAATATG CAATTATTGA TCCTATTTAT TCTAACGATC CTATTCCTGC AAATTTGGTG GGCATGACCT TTAGAGCTCA AGAGGGTGCA TTTTTAACGG GTTATATTGC TGCAAAACTT TCTAAAACAG GTAAAATTGG ATTTTTAGGG GGAATAGAAG GCGAGATAGT AGATGCTTTT AGGTATGGGT ATGAAGCTGG TGCTAAGTAT GCTAATAAAG ATATAAAGAT ATCTACTCAG TATATTGGTA GTTTTGCTGA CCTTGAAGCT GGTAGAAGCG TTGCAACTAG AATGTATTCT GATGAGATAG ACATTATTCA TCATGCTGCA GGCCTTGGAG GAATTGGGGC TATTGAGGTT GCAAAAGAAC TTGGTTCTGG GCATTACATT ATTGGAGTTG ATGAAGATCA AGCATATCTT GCTCCTGACA ATGTAATAAC ATCTACAACT AAAGATGTTG GTAGAGCTTT AAATATTTTT ACATCTAACC ATTTAAAAAC TAATACTTTC GAAGGTGGCA AATTAATAAA TTATGGCCTT AAAGAAGGAG TTGTGGGGTT TGTAAGAAAT CCTAAAATGA TTTCCTTTGA ACTTGAAAAA GAAATTGACA ATCTTTCTAG CAAAATAATC AACAAAGAAA TTATTGTTCC ATCTAATAAA GAAAGTTATG AGAAGTTTCT TAAAGAATTT ATTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT